Click here to switch to the map view.

The map label for this gene is kefA [H]

Identifier: 78223778

GI number: 78223778

Start: 2932552

End: 2933448

Strand: Reverse

Name: kefA [H]

Synonym: Gmet_2581

Alternate gene names: 78223778

Gene position: 2933448-2932552 (Counterclockwise)

Preceding gene: 78223779

Following gene: 78223777

Centisome position: 73.38

GC content: 58.97

Gene sequence:

>897_bases
ATGGAAACCGTTCGCGACATCCTGATTCAGATCAAGCAATTCCTCGAAGTCCCCCTCGTGAAGCTGGGCAGTGCGCCGGT
GACCCTCTGGACCATTATCCAGCTCGTGGTGCTGGTGGGGCTCCTCTTCTACCTCTCGGGAAAACTCCGGGGCTGGATCG
TGGATCAGTTCCTCACCCGGACGAGGATGGAGCTGGGGGCTCGCCAGGCCACCGGCTCCATTATCCGTTACACCATCATT
GCCATCGGTTTCATCATCATTCTCCAGACCGCCGGCATCGACCTCACCACCCTTAACGTCTTGGCCGGCGCCGTGGGAAT
CGGTGTCGGTTTCGGCCTCCAGAACATCGTCAACAATCTCGTTTGTGGCATCCTCATCCTCTTTGAGCGCCCCATCAAGG
TGGGGGATCGGATCGTGGTGGGCAACGTGGAGGGTGACGTGGTGCATATCGGCGGCAGAAGCACCACGGTGGTGACCAAC
GACAACATTACGATCATTGTCCCCAACTCGAAGTTCATCACCGAGAATGTGGTGAACTGGAGCCACAACGAGCGGAAGGT
CCGATTCCGGATACCGGTCAGCGTCGCCTACGAAAGCGATGTCCAGCAGGTGGAGCGGCTGCTGCTGGATGTTGCGGCTG
CAAACCCGGATGTGCTGGAAAAGCCGGCTCCCGGGGTTCGGCTCATGGAGTTCGGCGACAATGGCCTCAACTTCGAGCTC
AGGGTCTGGAGCACCACCCTCATTCACCGGCGAGGTCTCCTGACGAGCAACCTCAACTATGCCGTTTACCGCGCCTTTGT
CGAACATGGAATCGAGATACCCTTTCCTCGCCGTGATGTCCGTATCCTGCGTGAGGCGCGTCAGGCGGAACCGTTTGACG
GCAACGGCAGTTCGTGA

Upstream 100 bases:

>100_bases
GCTCGACCGGGGTTAGTGTCGCACTTGTCTGGGTGAATCTCCGTGGGCGTTGACAACGTTCTTAATCCCCCGGTTCTGGC
CTTTATAGCAAGGAGGCTTG

Downstream 100 bases:

>100_bases
GGGCGCCTGCTGTTACGTGAGGACGCTGAGCGTGGCCGATAACGTGGCAGGTGCACGTTTTGCCTGCTATACTCTCTCCC
CATGAGCCACGAATCGATTT

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
METVRDILIQIKQFLEVPLVKLGSAPVTLWTIIQLVVLVGLLFYLSGKLRGWIVDQFLTRTRMELGARQATGSIIRYTII
AIGFIIILQTAGIDLTTLNVLAGAVGIGVGFGLQNIVNNLVCGILILFERPIKVGDRIVVGNVEGDVVHIGGRSTTVVTN
DNITIIVPNSKFITENVVNWSHNERKVRFRIPVSVAYESDVQQVERLLLDVAAANPDVLEKPAPGVRLMEFGDNGLNFEL
RVWSTTLIHRRGLLTSNLNYAVYRAFVEHGIEIPFPRRDVRILREARQAEPFDGNGSS

Sequences:

>Translated_298_residues
METVRDILIQIKQFLEVPLVKLGSAPVTLWTIIQLVVLVGLLFYLSGKLRGWIVDQFLTRTRMELGARQATGSIIRYTII
AIGFIIILQTAGIDLTTLNVLAGAVGIGVGFGLQNIVNNLVCGILILFERPIKVGDRIVVGNVEGDVVHIGGRSTTVVTN
DNITIIVPNSKFITENVVNWSHNERKVRFRIPVSVAYESDVQQVERLLLDVAAANPDVLEKPAPGVRLMEFGDNGLNFEL
RVWSTTLIHRRGLLTSNLNYAVYRAFVEHGIEIPFPRRDVRILREARQAEPFDGNGSS
>Mature_298_residues
METVRDILIQIKQFLEVPLVKLGSAPVTLWTIIQLVVLVGLLFYLSGKLRGWIVDQFLTRTRMELGARQATGSIIRYTII
AIGFIIILQTAGIDLTTLNVLAGAVGIGVGFGLQNIVNNLVCGILILFERPIKVGDRIVVGNVEGDVVHIGGRSTTVVTN
DNITIIVPNSKFITENVVNWSHNERKVRFRIPVSVAYESDVQQVERLLLDVAAANPDVLEKPAPGVRLMEFGDNGLNFEL
RVWSTTLIHRRGLLTSNLNYAVYRAFVEHGIEIPFPRRDVRILREARQAEPFDGNGSS

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=277, Percent_Identity=33.2129963898917, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI2367355, Length=217, Percent_Identity=34.1013824884793, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789291, Length=252, Percent_Identity=29.7619047619048, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787591, Length=210, Percent_Identity=27.6190476190476, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 33060; Mature: 33060

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVRDILIQIKQFLEVPLVKLGSAPVTLWTIIQLVVLVGLLFYLSGKLRGWIVDQFLTR
CCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TRMELGARQATGSIIRYTIIAIGFIIILQTAGIDLTTLNVLAGAVGIGVGFGLQNIVNNL
HHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VCGILILFERPIKVGDRIVVGNVEGDVVHIGGRSTTVVTNDNITIIVPNSKFITENVVNW
HHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCEEEEECCCCCHHHHHHCC
SHNERKVRFRIPVSVAYESDVQQVERLLLDVAAANPDVLEKPAPGVRLMEFGDNGLNFEL
CCCCCEEEEEECEEEECHHHHHHHHHHHHHHHCCCCCHHCCCCCCCEEEEECCCCCEEEE
RVWSTTLIHRRGLLTSNLNYAVYRAFVEHGIEIPFPRRDVRILREARQAEPFDGNGSS
EEEHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
METVRDILIQIKQFLEVPLVKLGSAPVTLWTIIQLVVLVGLLFYLSGKLRGWIVDQFLTR
CCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TRMELGARQATGSIIRYTIIAIGFIIILQTAGIDLTTLNVLAGAVGIGVGFGLQNIVNNL
HHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VCGILILFERPIKVGDRIVVGNVEGDVVHIGGRSTTVVTNDNITIIVPNSKFITENVVNW
HHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCEEEEECCCCCHHHHHHCC
SHNERKVRFRIPVSVAYESDVQQVERLLLDVAAANPDVLEKPAPGVRLMEFGDNGLNFEL
CCCCCEEEEEECEEEECHHHHHHHHHHHHHHHCCCCCHHCCCCCCCEEEEECCCCCEEEE
RVWSTTLIHRRGLLTSNLNYAVYRAFVEHGIEIPFPRRDVRILREARQAEPFDGNGSS
EEEHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]