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The map label for this gene is dus1 [H]

Identifier: 78223758

GI number: 78223758

Start: 2907726

End: 2908700

Strand: Direct

Name: dus1 [H]

Synonym: Gmet_2561

Alternate gene names: 78223758

Gene position: 2907726-2908700 (Clockwise)

Preceding gene: 78223756

Following gene: 78223759

Centisome position: 72.74

GC content: 68.21

Gene sequence:

>975_bases
ATGCTCAACCCCCTGGCCATCGGCCCCCTGACCCTGGCAAACAATCTGATCCTCGCCCCCATGGCGGGAATTACGAACCT
CCCCTTCCGGCTTCTGGCCCGGGAGGAGGGGGCGAGCCTCTGCTTTACCGAGATGGTGAGCGTGAACGGCCTCGTGCGGG
AGGGGCGCAAGACCTTCGAGCTTCTGCGAAGCGTGCCGGAGGACCGTCCCCTGGGGATCCAGCTCTTCGGCGACGACCCG
CAGCTTCTGGCCGAAGGGGCACGGATGGTGGAGGGGCACGGCGACCTCATCGACATCAACATGGGATGCCCGGTCAAGAA
GGTGGTCGGCACCGGGGCCGGCAGCGCCCTGTTGCGGGAGCCGGCAAAGGTCCGGGCCATCATCCGTGAGACCCGCCGCG
CCACACGCCTTCCCCTGACCGTCAAGATCCGCAGCGGCTGGGCCTGCGGCGAGGCCAACTTCCTTGATATCGCCCAGATT
GCCGAGGAGGAGGGGTGCGACGCCATCACCCTCCACCCCCGGAGCCGGTCCCAGATGTTCGAGGGGCACGCCGACTGGCA
GCAGATCGCCGAGATGAAGCAGAGCGTGAAAATCCCCGTCATCGGCAGCGGCGATCTCTTCACCGCCGCCGACGTCACCG
CCATGCTGGCCGAAACCGGCTGCGACGGAGTCATGGTGGCCCGGGGCGCCCTGGGTAACCCGTGGATCTTCCGCCAGGCC
CTGGCCCTCCTGGACGGCAGGGAACCGGACGCACCCTCCACCGGGGAGCGGCTCCGCGCCGCGCTCCGACACCTGGAGCT
CTTCACGGAGCTCGCCGGCGAGCGGGTGGCCCTGCGGGAGATGCGCAAGCACTTCAGCTGGTATTCCCACGGTCTCTCCG
GCGCGGCCCAGTTCCGCAAGGCCGTCAACATCATCGAGGGAAAGGAAGCCCTTCTCGAAGCGCTGCACCGCTTCTTCGCG
CCGGAGGCGCCATGA

Upstream 100 bases:

>100_bases
CCCCCTGTCATAGCCGGGAAATATTCCTTGCCTTTTTTGCCCCCCGTGCTACCATGCTTAAATTTTAAGCACATCATCGC
CACACGAATAGAAATAACCA

Downstream 100 bases:

>100_bases
GCCCGGAAAGCCCCAACGAAGCGTTCCTTGCCACCATCATCGACAGCGTCGGCGACGGGGTCATCGTCATCGACCTGGGG
GGGCGGATCACCCTCATGAA

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MLNPLAIGPLTLANNLILAPMAGITNLPFRLLAREEGASLCFTEMVSVNGLVREGRKTFELLRSVPEDRPLGIQLFGDDP
QLLAEGARMVEGHGDLIDINMGCPVKKVVGTGAGSALLREPAKVRAIIRETRRATRLPLTVKIRSGWACGEANFLDIAQI
AEEEGCDAITLHPRSRSQMFEGHADWQQIAEMKQSVKIPVIGSGDLFTAADVTAMLAETGCDGVMVARGALGNPWIFRQA
LALLDGREPDAPSTGERLRAALRHLELFTELAGERVALREMRKHFSWYSHGLSGAAQFRKAVNIIEGKEALLEALHRFFA
PEAP

Sequences:

>Translated_324_residues
MLNPLAIGPLTLANNLILAPMAGITNLPFRLLAREEGASLCFTEMVSVNGLVREGRKTFELLRSVPEDRPLGIQLFGDDP
QLLAEGARMVEGHGDLIDINMGCPVKKVVGTGAGSALLREPAKVRAIIRETRRATRLPLTVKIRSGWACGEANFLDIAQI
AEEEGCDAITLHPRSRSQMFEGHADWQQIAEMKQSVKIPVIGSGDLFTAADVTAMLAETGCDGVMVARGALGNPWIFRQA
LALLDGREPDAPSTGERLRAALRHLELFTELAGERVALREMRKHFSWYSHGLSGAAQFRKAVNIIEGKEALLEALHRFFA
PEAP
>Mature_324_residues
MLNPLAIGPLTLANNLILAPMAGITNLPFRLLAREEGASLCFTEMVSVNGLVREGRKTFELLRSVPEDRPLGIQLFGDDP
QLLAEGARMVEGHGDLIDINMGCPVKKVVGTGAGSALLREPAKVRAIIRETRRATRLPLTVKIRSGWACGEANFLDIAQI
AEEEGCDAITLHPRSRSQMFEGHADWQQIAEMKQSVKIPVIGSGDLFTAADVTAMLAETGCDGVMVARGALGNPWIFRQA
LALLDGREPDAPSTGERLRAALRHLELFTELAGERVALREMRKHFSWYSHGLSGAAQFRKAVNIIEGKEALLEALHRFFA
PEAP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=307, Percent_Identity=30.9446254071661, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI239788483, Length=254, Percent_Identity=34.251968503937, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=34.4398340248963, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=31.5315315315315, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI8923374, Length=288, Percent_Identity=30.2083333333333, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=43.125, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1788462, Length=248, Percent_Identity=34.6774193548387, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693211, Length=313, Percent_Identity=26.8370607028754, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=264, Percent_Identity=31.0606060606061, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17507177, Length=242, Percent_Identity=31.8181818181818, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=30.4878048780488, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=276, Percent_Identity=26.4492753623188, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323433, Length=261, Percent_Identity=29.8850574712644, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=234, Percent_Identity=29.4871794871795, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585320, Length=249, Percent_Identity=34.136546184739, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=35.1111111111111, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=35.1111111111111, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921524, Length=308, Percent_Identity=27.5974025974026, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45549423, Length=248, Percent_Identity=31.8548387096774, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35290; Mature: 35290

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPLAIGPLTLANNLILAPMAGITNLPFRLLAREEGASLCFTEMVSVNGLVREGRKTFE
CCCCCCCCHHHHHCCCCEECCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LLRSVPEDRPLGIQLFGDDPQLLAEGARMVEGHGDLIDINMGCPVKKVVGTGAGSALLRE
HHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCHHHHHC
PAKVRAIIRETRRATRLPLTVKIRSGWACGEANFLDIAQIAEEEGCDAITLHPRSRSQMF
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHH
EGHADWQQIAEMKQSVKIPVIGSGDLFTAADVTAMLAETGCDGVMVARGALGNPWIFRQA
CCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
LALLDGREPDAPSTGERLRAALRHLELFTELAGERVALREMRKHFSWYSHGLSGAAQFRK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
AVNIIEGKEALLEALHRFFAPEAP
HHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNPLAIGPLTLANNLILAPMAGITNLPFRLLAREEGASLCFTEMVSVNGLVREGRKTFE
CCCCCCCCHHHHHCCCCEECCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LLRSVPEDRPLGIQLFGDDPQLLAEGARMVEGHGDLIDINMGCPVKKVVGTGAGSALLRE
HHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCHHHHHC
PAKVRAIIRETRRATRLPLTVKIRSGWACGEANFLDIAQIAEEEGCDAITLHPRSRSQMF
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHH
EGHADWQQIAEMKQSVKIPVIGSGDLFTAADVTAMLAETGCDGVMVARGALGNPWIFRQA
CCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
LALLDGREPDAPSTGERLRAALRHLELFTELAGERVALREMRKHFSWYSHGLSGAAQFRK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
AVNIIEGKEALLEALHRFFAPEAP
HHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]