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The map label for this gene is fabI [H]

Identifier: 78223755

GI number: 78223755

Start: 2904679

End: 2905449

Strand: Reverse

Name: fabI [H]

Synonym: Gmet_2558

Alternate gene names: 78223755

Gene position: 2905449-2904679 (Counterclockwise)

Preceding gene: 78223757

Following gene: 78223754

Centisome position: 72.68

GC content: 61.61

Gene sequence:

>771_bases
ATGGGGCTTTTGGATGGCAAGAAGGCGGTCATTTTCGGTATCGCCAACGAGAAGAGCATCGCATGGGCCATTGCCCAGGC
GTTCCGTCGCGAAGGGGCCGAACTGGCCGTAACCTATGCCAACGAGACGGTGGCGAAGCGGGTGATCCCCCTGGCGGAGA
GCGTCGGGGCGTCACTCGTGCTCCCCTGTGACGTGCGCAACGACGCGGAGATACAGAGTGTCTTTGAAGAGATAGGAAAG
GCGTGGGGGGGGATCGATATCCTCGTCCATTCCATCGCCTTCGCCGGCAAGGAGGAGCTCAAGGGATCGTTCCTCAATAC
GACCCGCGAGGGGTTTGCCCTTGCCCTCGATATCAGTGCCTACTCCCTCATTGCCCTCATGAAGGCGGCCCACCCCTTCA
TGGCGGGAAGGGAGGGGAGTGTTCTCGCGCTTACCTACTATGGCGGCCAGAAGGTTTTCCCGAACTACAACGTGATGGGG
GTTGCCAAGGCCGCTTTGGAGATGAGCGTCAAATACCTGGCCGAGGCCGTGGGTTCCGACGGGATCCGGGTGAACGCCAT
TTCTGCCGGCCCCCTGAAGACCCTGGCTTCATCCGGGGTGGGGGGATTCAATCAGATCGCCGGCCACGTGGCGGAAAAGG
CGCCCCTTCGCCGCAATATCAGCCAGGACGAGGTGGCAGGGGCGGCCGTCTACCTGGCCAGCTCCCTTGCGAGCGGCGTT
TCCGGCGAGATTCACTTCGTGGACAGCGGCTATAACATCATCGGGCTGTAA

Upstream 100 bases:

>100_bases
CGGGCGTCGAACTCCCTTGTCGCCGAGAAGATTATCAACATTGTCAGGAACGAAATGGATGGCGCCGGAAAGCGCCGTCT
CTGTTTCAGGAGGAATCTGA

Downstream 100 bases:

>100_bases
TACCGGGAACCGGTAAAGGCGGAGAACGAATATCAAACAGCTGCACGGCAACCTCATCGGCCTGAAGCCGAATCAGATCG
CATCCCTGGAGCGGCTCTAC

Product: enoyl-[acyl-carrier-protein] reductase [NADH]

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MGLLDGKKAVIFGIANEKSIAWAIAQAFRREGAELAVTYANETVAKRVIPLAESVGASLVLPCDVRNDAEIQSVFEEIGK
AWGGIDILVHSIAFAGKEELKGSFLNTTREGFALALDISAYSLIALMKAAHPFMAGREGSVLALTYYGGQKVFPNYNVMG
VAKAALEMSVKYLAEAVGSDGIRVNAISAGPLKTLASSGVGGFNQIAGHVAEKAPLRRNISQDEVAGAAVYLASSLASGV
SGEIHFVDSGYNIIGL

Sequences:

>Translated_256_residues
MGLLDGKKAVIFGIANEKSIAWAIAQAFRREGAELAVTYANETVAKRVIPLAESVGASLVLPCDVRNDAEIQSVFEEIGK
AWGGIDILVHSIAFAGKEELKGSFLNTTREGFALALDISAYSLIALMKAAHPFMAGREGSVLALTYYGGQKVFPNYNVMG
VAKAALEMSVKYLAEAVGSDGIRVNAISAGPLKTLASSGVGGFNQIAGHVAEKAPLRRNISQDEVAGAAVYLASSLASGV
SGEIHFVDSGYNIIGL
>Mature_255_residues
GLLDGKKAVIFGIANEKSIAWAIAQAFRREGAELAVTYANETVAKRVIPLAESVGASLVLPCDVRNDAEIQSVFEEIGKA
WGGIDILVHSIAFAGKEELKGSFLNTTREGFALALDISAYSLIALMKAAHPFMAGREGSVLALTYYGGQKVFPNYNVMGV
AKAALEMSVKYLAEAVGSDGIRVNAISAGPLKTLASSGVGGFNQIAGHVAEKAPLRRNISQDEVAGAAVYLASSLASGVS
GEIHFVDSGYNIIGL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031737, Length=243, Percent_Identity=27.1604938271605, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI10190704, Length=263, Percent_Identity=27.3764258555133, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=47.244094488189, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=29.0196078431373, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=28.735632183908, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=28.1124497991968, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367365, Length=267, Percent_Identity=28.0898876404494, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI23397609, Length=238, Percent_Identity=29.8319327731092, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 26750; Mature: 26619

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLDGKKAVIFGIANEKSIAWAIAQAFRREGAELAVTYANETVAKRVIPLAESVGASLV
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCEEE
LPCDVRNDAEIQSVFEEIGKAWGGIDILVHSIAFAGKEELKGSFLNTTREGFALALDISA
EEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCHHHCCCCCCEEEEEEHHH
YSLIALMKAAHPFMAGREGSVLALTYYGGQKVFPNYNVMGVAKAALEMSVKYLAEAVGSD
HHHHHHHHHHCHHHCCCCCCEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
GIRVNAISAGPLKTLASSGVGGFNQIAGHVAEKAPLRRNISQDEVAGAAVYLASSLASGV
CEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHCCC
SGEIHFVDSGYNIIGL
CCEEEEEECCCEEECC
>Mature Secondary Structure 
GLLDGKKAVIFGIANEKSIAWAIAQAFRREGAELAVTYANETVAKRVIPLAESVGASLV
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCEEE
LPCDVRNDAEIQSVFEEIGKAWGGIDILVHSIAFAGKEELKGSFLNTTREGFALALDISA
EEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCHHHCCCCCCEEEEEEHHH
YSLIALMKAAHPFMAGREGSVLALTYYGGQKVFPNYNVMGVAKAALEMSVKYLAEAVGSD
HHHHHHHHHHCHHHCCCCCCEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
GIRVNAISAGPLKTLASSGVGGFNQIAGHVAEKAPLRRNISQDEVAGAAVYLASSLASGV
CEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHCCC
SGEIHFVDSGYNIIGL
CCEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]