Click here to switch to the map view.

The map label for this gene is mltD [H]

Identifier: 78223753

GI number: 78223753

Start: 2901389

End: 2902888

Strand: Reverse

Name: mltD [H]

Synonym: Gmet_2556

Alternate gene names: 78223753

Gene position: 2902888-2901389 (Counterclockwise)

Preceding gene: 78223754

Following gene: 78223752

Centisome position: 72.62

GC content: 60.0

Gene sequence:

>1500_bases
ATGAAAAAAGCTCTTCTCCTCCTCGTTCTATGTCTCTTTCCGGTTCTTCCCGCCCTGGCATCGGAACCCCGTAACTCACC
CCTCAAGGAACTGGCCGCGCTGGGTAATTCCTCCTCGCTTCCCGACCTTTCGGGAGCCGTTCCCTCCGGGTTCGACTCTC
GCCCCGGCCGCAGGTCCGGTTCCCTTGAGCCCTCCATGGGAGACCTGGTCGTCATCGAAGACCAGGGAGCCGCCGAAAAC
GACTTTGAACTGAAGATTCCCGAGGGTCCGCTCCCCGACTCGGATATTCCGCTTACCCTCAACGGCCAGGTGGAGTACTT
CATCCGCTACTTCCAGTCATCCGGCCGCAAGTCTTTTACCCGCTGGCTTTCCCGCTCCGAACGCTATATTCCGATGATGA
AGGAAGTCCTCAAGAAAAACGGCCTTCCCGAGGATCTGGTCTACCTCGCCATGATCGAGAGCGGCTTCACCCCCCACGCG
GTGTCGGTGGCTAGCGCCGTGGGGCCGTGGCAGTTCATCTCCGGCACCGGGAAACGCTATGATCTGCGGATAGACCAGTG
GATCGACGAGCGGCGGGATCCCCTCAAGTCGACGGTGGCGGCGGCCCTGTACCTGAAAGAGCTCTACGCCATGTTCAACA
ACGACTGGTACCTGGCGGCAGCAGGGTACAACGCGGGGGAGAACAAAATCCTGCGGGCCATTGATCGGTACAATACCCGC
GACTTCTGGGAGATCTCCAAGGGTTCATATCTGAAGCGGGAAACCAAGGAGTACGTGCCGAAACTCCTGGCCGCCGCCAT
TATCGCCAAGGAACCGGCCCGTTATGGCTTTGCCGACGTTGCCTACCTTCCTCCCATCGAGTTCGACACGGTGACGATTC
CCTCCCGCACCGATCTGGAGGTGGCGGCGAAGCTCTGCAATGTGGACTATCAGGTCGTCAAGGAGCTGAATCCTGAACTG
CGCCGCTGGTGCACTCCCCCGGACTACCCCGGCTACGAGCTTAAGATTCCCGTTGGCAAGAAGAGGGCCTTTGAGCAGGC
CTATGCGCAACTTCCCGAAGGTCAGCGCTACGCCGAGCGGGTTGTTTACACCCGCTACCGGGCCAAGAAAAGGGATACCC
TCGCGTCAATCGCCAGCCGTTACGGCACCACCCCCCAGGCCCTGGCCGAGGTGAACAAGCTGAAGCTCTCCGCCAAGGTG
CGGGGGCGGACCCTCCTTGTGCCGGTGGTTGCCGACAGGGAGCCGGCGGTCCAGACCGCGAAGGCCGATGTTTCGCGCAA
GGAGAGCGCCAAGGGGTTCAACAAGTACTACACCGTGAAAAAGGGCGACACGATTGCGTCGCTTTCAAAGAAATTCAATA
TCTCGTCCCGCATTCTCACCGCCTGGAACAGCCTGAAGGGAAAACTTGCCCTTCGTCCCGGCAAGCGGATCATCGTTGCC
AAGTACGTGGAGAAGAAGGGTTCCATGGTTCCGGTGTCCGGCGGCGACGACAACGGATAA

Upstream 100 bases:

>100_bases
CCCCAATCTTGACACCAATGCCCCGATTCCCTATAATGCGGGTTCCGTCCGGCACTACTTCCCACATCCGGTCGTTATGG
CCATGCGTGGAAGGTTTCTC

Downstream 100 bases:

>100_bases
ACAGGCCAAGCTCTACGCAGCATGAAAGGACATGAATTTTTATGTATAACTTCCTGATCTCCGGCGGCATCGCCCTCATT
GCAATCATCATCATCTTCAT

Product: peptidoglycan-binding LysM:lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKKALLLLVLCLFPVLPALASEPRNSPLKELAALGNSSSLPDLSGAVPSGFDSRPGRRSGSLEPSMGDLVVIEDQGAAEN
DFELKIPEGPLPDSDIPLTLNGQVEYFIRYFQSSGRKSFTRWLSRSERYIPMMKEVLKKNGLPEDLVYLAMIESGFTPHA
VSVASAVGPWQFISGTGKRYDLRIDQWIDERRDPLKSTVAAALYLKELYAMFNNDWYLAAAGYNAGENKILRAIDRYNTR
DFWEISKGSYLKRETKEYVPKLLAAAIIAKEPARYGFADVAYLPPIEFDTVTIPSRTDLEVAAKLCNVDYQVVKELNPEL
RRWCTPPDYPGYELKIPVGKKRAFEQAYAQLPEGQRYAERVVYTRYRAKKRDTLASIASRYGTTPQALAEVNKLKLSAKV
RGRTLLVPVVADREPAVQTAKADVSRKESAKGFNKYYTVKKGDTIASLSKKFNISSRILTAWNSLKGKLALRPGKRIIVA
KYVEKKGSMVPVSGGDDNG

Sequences:

>Translated_499_residues
MKKALLLLVLCLFPVLPALASEPRNSPLKELAALGNSSSLPDLSGAVPSGFDSRPGRRSGSLEPSMGDLVVIEDQGAAEN
DFELKIPEGPLPDSDIPLTLNGQVEYFIRYFQSSGRKSFTRWLSRSERYIPMMKEVLKKNGLPEDLVYLAMIESGFTPHA
VSVASAVGPWQFISGTGKRYDLRIDQWIDERRDPLKSTVAAALYLKELYAMFNNDWYLAAAGYNAGENKILRAIDRYNTR
DFWEISKGSYLKRETKEYVPKLLAAAIIAKEPARYGFADVAYLPPIEFDTVTIPSRTDLEVAAKLCNVDYQVVKELNPEL
RRWCTPPDYPGYELKIPVGKKRAFEQAYAQLPEGQRYAERVVYTRYRAKKRDTLASIASRYGTTPQALAEVNKLKLSAKV
RGRTLLVPVVADREPAVQTAKADVSRKESAKGFNKYYTVKKGDTIASLSKKFNISSRILTAWNSLKGKLALRPGKRIIVA
KYVEKKGSMVPVSGGDDNG
>Mature_499_residues
MKKALLLLVLCLFPVLPALASEPRNSPLKELAALGNSSSLPDLSGAVPSGFDSRPGRRSGSLEPSMGDLVVIEDQGAAEN
DFELKIPEGPLPDSDIPLTLNGQVEYFIRYFQSSGRKSFTRWLSRSERYIPMMKEVLKKNGLPEDLVYLAMIESGFTPHA
VSVASAVGPWQFISGTGKRYDLRIDQWIDERRDPLKSTVAAALYLKELYAMFNNDWYLAAAGYNAGENKILRAIDRYNTR
DFWEISKGSYLKRETKEYVPKLLAAAIIAKEPARYGFADVAYLPPIEFDTVTIPSRTDLEVAAKLCNVDYQVVKELNPEL
RRWCTPPDYPGYELKIPVGKKRAFEQAYAQLPEGQRYAERVVYTRYRAKKRDTLASIASRYGTTPQALAEVNKLKLSAKV
RGRTLLVPVVADREPAVQTAKADVSRKESAKGFNKYYTVKKGDTIASLSKKFNISSRILTAWNSLKGKLALRPGKRIIVA
KYVEKKGSMVPVSGGDDNG

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=372, Percent_Identity=31.1827956989247, Blast_Score=156, Evalue=3e-39,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 55655; Mature: 55655

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALLLLVLCLFPVLPALASEPRNSPLKELAALGNSSSLPDLSGAVPSGFDSRPGRRSG
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SLEPSMGDLVVIEDQGAAENDFELKIPEGPLPDSDIPLTLNGQVEYFIRYFQSSGRKSFT
CCCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCEEECCHHHHHHHHHHHCCHHHHH
RWLSRSERYIPMMKEVLKKNGLPEDLVYLAMIESGFTPHAVSVASAVGPWQFISGTGKRY
HHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHCCCCCEE
DLRIDQWIDERRDPLKSTVAAALYLKELYAMFNNDWYLAAAGYNAGENKILRAIDRYNTR
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCC
DFWEISKGSYLKRETKEYVPKLLAAAIIAKEPARYGFADVAYLPPIEFDTVTIPSRTDLE
CCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEECCCCCCHH
VAAKLCNVDYQVVKELNPELRRWCTPPDYPGYELKIPVGKKRAFEQAYAQLPEGQRYAER
HHHHHHCCHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHH
VVYTRYRAKKRDTLASIASRYGTTPQALAEVNKLKLSAKVRGRTLLVPVVADREPAVQTA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCEEEEEEECCCCCHHHHH
KADVSRKESAKGFNKYYTVKKGDTIASLSKKFNISSRILTAWNSLKGKLALRPGKRIIVA
HHHHHHHHHCCCCHHEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEE
KYVEKKGSMVPVSGGDDNG
EEHHHCCCEEEECCCCCCC
>Mature Secondary Structure
MKKALLLLVLCLFPVLPALASEPRNSPLKELAALGNSSSLPDLSGAVPSGFDSRPGRRSG
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SLEPSMGDLVVIEDQGAAENDFELKIPEGPLPDSDIPLTLNGQVEYFIRYFQSSGRKSFT
CCCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCEEECCHHHHHHHHHHHCCHHHHH
RWLSRSERYIPMMKEVLKKNGLPEDLVYLAMIESGFTPHAVSVASAVGPWQFISGTGKRY
HHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHCCCCCEE
DLRIDQWIDERRDPLKSTVAAALYLKELYAMFNNDWYLAAAGYNAGENKILRAIDRYNTR
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCC
DFWEISKGSYLKRETKEYVPKLLAAAIIAKEPARYGFADVAYLPPIEFDTVTIPSRTDLE
CCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEECCCCCCHH
VAAKLCNVDYQVVKELNPELRRWCTPPDYPGYELKIPVGKKRAFEQAYAQLPEGQRYAER
HHHHHHCCHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHH
VVYTRYRAKKRDTLASIASRYGTTPQALAEVNKLKLSAKVRGRTLLVPVVADREPAVQTA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCEEEEEEECCCCCHHHHH
KADVSRKESAKGFNKYYTVKKGDTIASLSKKFNISSRILTAWNSLKGKLALRPGKRIIVA
HHHHHHHHHCCCCHHEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEE
KYVEKKGSMVPVSGGDDNG
EEHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]