Click here to switch to the map view.

The map label for this gene is cmoB

Identifier: 78223730

GI number: 78223730

Start: 2873755

End: 2874726

Strand: Reverse

Name: cmoB

Synonym: Gmet_2531

Alternate gene names: 78223730

Gene position: 2874726-2873755 (Counterclockwise)

Preceding gene: 78223731

Following gene: 78223729

Centisome position: 71.91

GC content: 64.71

Gene sequence:

>972_bases
ATGAGCTGGTACGACGCTCTCTTTCCGCACCTTGCCGCCATGGGGCAGGAGCGCTGGGCCGAGCAGTTGCAGGTGACCCT
GTCCGAGAGCCAGCTCCTCCAACGGTACGGCGATATGCCGGGCTGGCTGAGCGCGCTGCAGGCGCTGCCGGAGCTGCACC
CATCCCGGATTGCACTGCAGGACACTGTCACCATCGGTTCCGGCGCCGATCTCTGCTCAGTCACTCGCGAAGATCTCATC
CTCGGGCTGCAGGCCTTTCACCCCTGGCGCAAGGGCCCCTACAGTGTCTTCGGCATCGAGATCGATACCGAGTGGCGCTC
CGACTGGAAGTGGGAGCGCCTGCTGCCGCACATTCAGCCGCTCGCCGGGCGCAAAGTCCTGGATGTCGGCTGCGGCAACG
GCTATCATGGCTGGCGTATGCGCGGCGCCGGCGCCGACTTCGTCCTCGGCATCGAGCCCTTTCTGGTTTCGGTGATGCAG
TTCCAGGTGATGCAGCGCTACCTGCGGGATCCCCGGCATCATGTCATCCCCATCGGCATTGAGGATGTCCCGCCGAATCC
GGCCTGCTTCGACAGTGTCTTCTCCATGGGGGTCCTCTACCATCGGCGTTCTCCCCTGGATCATCTTCTGGAGTTGAAGG
GGTGCCTGCGCCCGGGAGGGGAGCTGATTCTGGAGACGCTGATTGTCGAGGGGGGGCAGGAGACGATCTTCATGCCGCCG
GGACGCTACGCCAAGATGCGCAATGTCTGGTTCCTCCCGTCGATTGCGGCGCTGACCCTTTGGCTGCAGCGCTGCGGCTT
CACGGAGATTGCCTGTGTCGACGCCACCCGCACCACCTGCGAGGAGCAGCGCGCGACGGAGTGGATGCGCTTCGAGTCGC
TGGCGGATTTCCTTGATCCGGATGACGGCGGGAAGACGATGGAAGGGCACCCGGCGCCGCTGCGGGCAATCTTTACCGCG
ACGAAGCCCTAG

Upstream 100 bases:

>100_bases
AGACCCTTGCCTGCCATCATGAGCGCCTGCAGGCGGCGGGCTTTTCGTTCTCCGAGCTCTGGTTCCAGTGTTTCAACTTT
GCCTCTCTGGTGGCGATGAA

Downstream 100 bases:

>100_bases
GGGTGCGGGGGGGCCCCCTTCGCGATACAATGGAAACCATCGCCCGTCAGTACGGCTGCGAGGACTTGGTACGGGTATCC
GGCAACGACGTGCCGGTGCG

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSWYDALFPHLAAMGQERWAEQLQVTLSESQLLQRYGDMPGWLSALQALPELHPSRIALQDTVTIGSGADLCSVTREDLI
LGLQAFHPWRKGPYSVFGIEIDTEWRSDWKWERLLPHIQPLAGRKVLDVGCGNGYHGWRMRGAGADFVLGIEPFLVSVMQ
FQVMQRYLRDPRHHVIPIGIEDVPPNPACFDSVFSMGVLYHRRSPLDHLLELKGCLRPGGELILETLIVEGGQETIFMPP
GRYAKMRNVWFLPSIAALTLWLQRCGFTEIACVDATRTTCEEQRATEWMRFESLADFLDPDDGGKTMEGHPAPLRAIFTA
TKP

Sequences:

>Translated_323_residues
MSWYDALFPHLAAMGQERWAEQLQVTLSESQLLQRYGDMPGWLSALQALPELHPSRIALQDTVTIGSGADLCSVTREDLI
LGLQAFHPWRKGPYSVFGIEIDTEWRSDWKWERLLPHIQPLAGRKVLDVGCGNGYHGWRMRGAGADFVLGIEPFLVSVMQ
FQVMQRYLRDPRHHVIPIGIEDVPPNPACFDSVFSMGVLYHRRSPLDHLLELKGCLRPGGELILETLIVEGGQETIFMPP
GRYAKMRNVWFLPSIAALTLWLQRCGFTEIACVDATRTTCEEQRATEWMRFESLADFLDPDDGGKTMEGHPAPLRAIFTA
TKP
>Mature_322_residues
SWYDALFPHLAAMGQERWAEQLQVTLSESQLLQRYGDMPGWLSALQALPELHPSRIALQDTVTIGSGADLCSVTREDLIL
GLQAFHPWRKGPYSVFGIEIDTEWRSDWKWERLLPHIQPLAGRKVLDVGCGNGYHGWRMRGAGADFVLGIEPFLVSVMQF
QVMQRYLRDPRHHVIPIGIEDVPPNPACFDSVFSMGVLYHRRSPLDHLLELKGCLRPGGELILETLIVEGGQETIFMPPG
RYAKMRNVWFLPSIAALTLWLQRCGFTEIACVDATRTTCEEQRATEWMRFESLADFLDPDDGGKTMEGHPAPLRAIFTAT
KP

Specific function: Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoB family

Homologues:

Organism=Escherichia coli, GI1788178, Length=321, Percent_Identity=49.5327102803738, Blast_Score=324, Evalue=5e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CMOB_GEOMG (Q39SM2)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385477.1
- ProteinModelPortal:   Q39SM2
- STRING:   Q39SM2
- GeneID:   3738582
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2531
- NMPDR:   fig|269799.3.peg.2695
- eggNOG:   COG0500
- HOGENOM:   HBG296882
- OMA:   DVIFCLG
- PhylomeDB:   Q39SM2
- ProtClustDB:   PRK15068
- BioCyc:   GMET269799:GMET_2531-MONOMER
- HAMAP:   MF_01590
- InterPro:   IPR010017
- TIGRFAMs:   TIGR00452

Pfam domain/function: PF08003 Methyltransf_9

EC number: NA

Molecular weight: Translated: 36529; Mature: 36397

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWYDALFPHLAAMGQERWAEQLQVTLSESQLLQRYGDMPGWLSALQALPELHPSRIALQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEE
DTVTIGSGADLCSVTREDLILGLQAFHPWRKGPYSVFGIEIDTEWRSDWKWERLLPHIQP
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCC
LAGRKVLDVGCGNGYHGWRMRGAGADFVLGIEPFLVSVMQFQVMQRYLRDPRHHVIPIGI
CCCCEEEEECCCCCCCCCEEECCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
EDVPPNPACFDSVFSMGVLYHRRSPLDHLLELKGCLRPGGELILETLIVEGGQETIFMPP
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECC
GRYAKMRNVWFLPSIAALTLWLQRCGFTEIACVDATRTTCEEQRATEWMRFESLADFLDP
CCCHHHCCEEECHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
DDGGKTMEGHPAPLRAIFTATKP
CCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
SWYDALFPHLAAMGQERWAEQLQVTLSESQLLQRYGDMPGWLSALQALPELHPSRIALQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEE
DTVTIGSGADLCSVTREDLILGLQAFHPWRKGPYSVFGIEIDTEWRSDWKWERLLPHIQP
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCC
LAGRKVLDVGCGNGYHGWRMRGAGADFVLGIEPFLVSVMQFQVMQRYLRDPRHHVIPIGI
CCCCEEEEECCCCCCCCCEEECCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
EDVPPNPACFDSVFSMGVLYHRRSPLDHLLELKGCLRPGGELILETLIVEGGQETIFMPP
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECC
GRYAKMRNVWFLPSIAALTLWLQRCGFTEIACVDATRTTCEEQRATEWMRFESLADFLDP
CCCHHHCCEEECHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
DDGGKTMEGHPAPLRAIFTATKP
CCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA