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The map label for this gene is pdhC [H]

Identifier: 78223711

GI number: 78223711

Start: 2851495

End: 2852658

Strand: Direct

Name: pdhC [H]

Synonym: Gmet_2511

Alternate gene names: 78223711

Gene position: 2851495-2852658 (Clockwise)

Preceding gene: 78223710

Following gene: 78223712

Centisome position: 71.33

GC content: 66.24

Gene sequence:

>1164_bases
ATGCCCTTTGACTTCAAACTCCCCGATCTCGGTGAAGGGATCACCGAAGCCGAGCTGAGAAAGTGGCTCGTGAAGGAAGG
CGACACCGTTCGCGAGCACCAGCCCGTGGCCGAGGTGGAAACCGACAAGGCGGTGGTGGAGGTTCCCTCTCCCCGGGGCG
GGCGGGTCGGCCGGCTGGCACGGCGCGAGGGGGAGACGGTGGCGGTGGGCGCGACGCTCTTCACCATCGAGGAGGAGGGT
GAAGCCCCGCCGGAGCGACCCAAATCAGTAGGAATCGTGGGGGAGCTCCCCGAGGCGGAGGAGGCGCGCGAGGTCATCGC
CACCCCCCTGGTAAGGAAACTGGCCCGGGAGCGGGGTATCGACCTGGCCACGGTCAGGGGAAGCGGCCCCCGGGGAAGCA
TCACCCCCGATGACCTGGAGAAAGTCTCAGCTCCGGTTGCCCAGCCCGCGGAGAGTTTCGGCCCCGTGGAACTGGTCCCC
CTGCGGGGGGTGCGCCGCGCCGTGGCCCGCAACGTCATGGCCTCCCAGCGGAACACCGCCTTCGTCACCGGCATGGAGGA
AGCTGACATCACCGAGCTCTGGGAGCTGCGCCGCCGCGAACTGGGGGCCGTTGAAACCCGGGGCGCCCACCTCACCTTCC
TCCCCTTCTTCATCAAGGCGGTCCAGCACGCACTTCGGGAGCACCCCTACCTGAACGCAGCCATCGACGACACGGCCGAG
ACCATCATACTCAAGCGCCACTACCACTTCGGCATCGCCGTGGAAACCCCCGACGGCCTCATGGTGCCGGTCATCCGCGA
CGTGGACCGGAAAAGCATCATCGAACTGGCGGCCGAAATCCAGGGACTGGGCTCCAAGGCCCGGAAACGGACCATCTCCC
TCGACGAGTTGAAGGGAAGCACCTTCACCCTCACCAACTACGGCCACTTCGGCGGCGTCTTTGCAACCCCCATCATCAAC
TGGCCCGACGTGGCCATCCTCGGCTTCGGTCGCATCGGCGAACGCCCCTGGATTCACAAGGGGCAGATCGCCATCCGCAG
AATCCTCCCCCTTTCCCTCACCTTCGACCACCGGGTCACGGACGGGGCCGACGCGGCCCAGTTCCTCCTCAAGGTGGTCG
CCTACCTGGAAGACCCGGCACTTCTTTTCATCGAAAGCACCTGA

Upstream 100 bases:

>100_bases
ACGCCGGTGCCCCTGGCGAAGCTGATCGACCACTACCTTCCGTCGCCGGAGCGGATTCGGGCGGCGCTCGATGACGTGCT
GCAGTACTGAGGAGCGACCC

Downstream 100 bases:

>100_bases
TGGTTTTTTCGCGTTAAAGTACTCGACAATACTGTCGAAACTTTTTACACAATTTTCCATTTCTCGATGACTGTGCCAAC
GAACCGTTGTCATATCGGGA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPFDFKLPDLGEGITEAELRKWLVKEGDTVREHQPVAEVETDKAVVEVPSPRGGRVGRLARREGETVAVGATLFTIEEEG
EAPPERPKSVGIVGELPEAEEAREVIATPLVRKLARERGIDLATVRGSGPRGSITPDDLEKVSAPVAQPAESFGPVELVP
LRGVRRAVARNVMASQRNTAFVTGMEEADITELWELRRRELGAVETRGAHLTFLPFFIKAVQHALREHPYLNAAIDDTAE
TIILKRHYHFGIAVETPDGLMVPVIRDVDRKSIIELAAEIQGLGSKARKRTISLDELKGSTFTLTNYGHFGGVFATPIIN
WPDVAILGFGRIGERPWIHKGQIAIRRILPLSLTFDHRVTDGADAAQFLLKVVAYLEDPALLFIEST

Sequences:

>Translated_387_residues
MPFDFKLPDLGEGITEAELRKWLVKEGDTVREHQPVAEVETDKAVVEVPSPRGGRVGRLARREGETVAVGATLFTIEEEG
EAPPERPKSVGIVGELPEAEEAREVIATPLVRKLARERGIDLATVRGSGPRGSITPDDLEKVSAPVAQPAESFGPVELVP
LRGVRRAVARNVMASQRNTAFVTGMEEADITELWELRRRELGAVETRGAHLTFLPFFIKAVQHALREHPYLNAAIDDTAE
TIILKRHYHFGIAVETPDGLMVPVIRDVDRKSIIELAAEIQGLGSKARKRTISLDELKGSTFTLTNYGHFGGVFATPIIN
WPDVAILGFGRIGERPWIHKGQIAIRRILPLSLTFDHRVTDGADAAQFLLKVVAYLEDPALLFIEST
>Mature_386_residues
PFDFKLPDLGEGITEAELRKWLVKEGDTVREHQPVAEVETDKAVVEVPSPRGGRVGRLARREGETVAVGATLFTIEEEGE
APPERPKSVGIVGELPEAEEAREVIATPLVRKLARERGIDLATVRGSGPRGSITPDDLEKVSAPVAQPAESFGPVELVPL
RGVRRAVARNVMASQRNTAFVTGMEEADITELWELRRRELGAVETRGAHLTFLPFFIKAVQHALREHPYLNAAIDDTAET
IILKRHYHFGIAVETPDGLMVPVIRDVDRKSIIELAAEIQGLGSKARKRTISLDELKGSTFTLTNYGHFGGVFATPIINW
PDVAILGFGRIGERPWIHKGQIAIRRILPLSLTFDHRVTDGADAAQFLLKVVAYLEDPALLFIEST

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=420, Percent_Identity=34.0476190476191, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI19923748, Length=223, Percent_Identity=38.1165919282511, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=27.1461716937355, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=24.7240618101545, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=24.5033112582781, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI260898739, Length=135, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786946, Length=402, Percent_Identity=32.089552238806, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1786305, Length=419, Percent_Identity=33.1742243436754, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17537937, Length=422, Percent_Identity=33.6492890995261, Blast_Score=215, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25146366, Length=409, Percent_Identity=31.2958435207824, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=28.3720930232558, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=228, Percent_Identity=29.3859649122807, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=400, Percent_Identity=32, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=24.4444444444444, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859875, Length=423, Percent_Identity=31.2056737588652, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=37.3333333333333, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24582497, Length=294, Percent_Identity=28.2312925170068, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI20129315, Length=294, Percent_Identity=28.2312925170068, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 42581; Mature: 42450

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFDFKLPDLGEGITEAELRKWLVKEGDTVREHQPVAEVETDKAVVEVPSPRGGRVGRLA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCHHCCCCCEEEECCCCCCCCCHHHH
RREGETVAVGATLFTIEEEGEAPPERPKSVGIVGELPEAEEAREVIATPLVRKLARERGI
HCCCCEEEEEEEEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
DLATVRGSGPRGSITPDDLEKVSAPVAQPAESFGPVELVPLRGVRRAVARNVMASQRNTA
EEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCE
FVTGMEEADITELWELRRRELGAVETRGAHLTFLPFFIKAVQHALREHPYLNAAIDDTAE
EEECCCCCCHHHHHHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHCCCCCCHHCCCHH
TIILKRHYHFGIAVETPDGLMVPVIRDVDRKSIIELAAEIQGLGSKARKRTISLDELKGS
EEEEEEEEEEEEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCC
TFTLTNYGHFGGVFATPIINWPDVAILGFGRIGERPWIHKGQIAIRRILPLSLTFDHRVT
EEEEECCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEHEEEEEEECCCCC
DGADAAQFLLKVVAYLEDPALLFIEST
CCCHHHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
PFDFKLPDLGEGITEAELRKWLVKEGDTVREHQPVAEVETDKAVVEVPSPRGGRVGRLA
CCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCHHCCCCCEEEECCCCCCCCCHHHH
RREGETVAVGATLFTIEEEGEAPPERPKSVGIVGELPEAEEAREVIATPLVRKLARERGI
HCCCCEEEEEEEEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
DLATVRGSGPRGSITPDDLEKVSAPVAQPAESFGPVELVPLRGVRRAVARNVMASQRNTA
EEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCE
FVTGMEEADITELWELRRRELGAVETRGAHLTFLPFFIKAVQHALREHPYLNAAIDDTAE
EEECCCCCCHHHHHHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHCCCCCCHHCCCHH
TIILKRHYHFGIAVETPDGLMVPVIRDVDRKSIIELAAEIQGLGSKARKRTISLDELKGS
EEEEEEEEEEEEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCC
TFTLTNYGHFGGVFATPIINWPDVAILGFGRIGERPWIHKGQIAIRRILPLSLTFDHRVT
EEEEECCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEHEEEEEEECCCCC
DGADAAQFLLKVVAYLEDPALLFIEST
CCCHHHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]