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The map label for this gene is ndoA [H]

Identifier: 78223703

GI number: 78223703

Start: 2844030

End: 2844359

Strand: Direct

Name: ndoA [H]

Synonym: Gmet_2503

Alternate gene names: 78223703

Gene position: 2844030-2844359 (Clockwise)

Preceding gene: 78223702

Following gene: 78223704

Centisome position: 71.15

GC content: 53.94

Gene sequence:

>330_bases
ATGGGAATGGTAGTCAAACGCTTCGACGTCTATCTGGTCAACCTGGACCCCACCGTCGGCAGGGAGATTCGCAAGGCAAG
GCCATGTCTCATTATCTCACCCGATGAAATGAACCGGTACATCGGCACCGTTATCGTTGCCCCCATGACCACCAAAGGGA
GGGATTATCCCACGCGGGTGCAGTGCTCCTTCAAGGGCAAGGAGGGACAGGTTGTCCTTGACCAGATCAGAACCGTGGAC
AAGTCCCGTCTTTTCCAGAAGCTCGGAAGAATCGACAAAGAAACGCAGGAAGAGGTGTTGTCCATTCTTGCGGAGATGTT
TGCGCCGTAA

Upstream 100 bases:

>100_bases
GCCAGGGGTGGGGGGAGAAATTCCAGCTCATGGCTGAGAGCGGCGATGACAGAATGCTTAACGGGGATGCGGGCGAGCAG
ACCGAATGGGACAAGGATGA

Downstream 100 bases:

>100_bases
GTAGGAGAGCCGGTGCGTCGAAAATGAACCACATGGGGAGTCGGCAACGGCTCCCCTTTTCCGTTGCTTGGATTGGACGC
CTTGACAGCGCGTCGCACCG

Product: transcriptional modulator of MazE/toxin, MazF

Products: NA

Alternate protein names: Endoribonuclease EndoA; Toxin EndoA [H]

Number of amino acids: Translated: 109; Mature: 108

Protein sequence:

>109_residues
MGMVVKRFDVYLVNLDPTVGREIRKARPCLIISPDEMNRYIGTVIVAPMTTKGRDYPTRVQCSFKGKEGQVVLDQIRTVD
KSRLFQKLGRIDKETQEEVLSILAEMFAP

Sequences:

>Translated_109_residues
MGMVVKRFDVYLVNLDPTVGREIRKARPCLIISPDEMNRYIGTVIVAPMTTKGRDYPTRVQCSFKGKEGQVVLDQIRTVD
KSRLFQKLGRIDKETQEEVLSILAEMFAP
>Mature_108_residues
GMVVKRFDVYLVNLDPTVGREIRKARPCLIISPDEMNRYIGTVIVAPMTTKGRDYPTRVQCSFKGKEGQVVLDQIRTVDK
SRLFQKLGRIDKETQEEVLSILAEMFAP

Specific function: Part of a toxin-antitoxin (TA) module. Has specificity for UAC sequences, yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 12447; Mature: 12316

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMVVKRFDVYLVNLDPTVGREIRKARPCLIISPDEMNRYIGTVIVAPMTTKGRDYPTRV
CCCEEEEEEEEEEECCCHHHHHHHHCCCEEEECHHHHHHHHHEEEEECCCCCCCCCCCEE
QCSFKGKEGQVVLDQIRTVDKSRLFQKLGRIDKETQEEVLSILAEMFAP
EEEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GMVVKRFDVYLVNLDPTVGREIRKARPCLIISPDEMNRYIGTVIVAPMTTKGRDYPTRV
CCEEEEEEEEEEECCCHHHHHHHHCCCEEEECHHHHHHHHHEEEEECCCCCCCCCCCEE
QCSFKGKEGQVVLDQIRTVDKSRLFQKLGRIDKETQEEVLSILAEMFAP
EEEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]