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The map label for this gene is macA [H]

Identifier: 78223611

GI number: 78223611

Start: 2722909

End: 2724123

Strand: Reverse

Name: macA [H]

Synonym: Gmet_2408

Alternate gene names: 78223611

Gene position: 2724123-2722909 (Counterclockwise)

Preceding gene: 78223612

Following gene: 78223610

Centisome position: 68.15

GC content: 65.43

Gene sequence:

>1215_bases
ATGGCCAACGAAGACCTGGGAAAGCTGAAGATCGACAAGGGCGCCCTTGCCCGTTCGGGAGGCCGGCGCCGGCCGCGCTT
CTGGATTGTGGCAGCTGTGGCGGTGGTTGTGCTCCTGATCCTCTTCGTGAGCGGCGTCCTTTCGCCGGCGGTAACCGTGG
AAACGGGCACCGTGACCCAGGTCTACCCTTCCCAGGCCTACACACTCCTGAACGCCAGCGGTTATGTGGTGGCCCAGCGC
AAGGCCGCCGTGGCCGCCAAGGTGACCGGTCGGCTTGACTGGCTGGGAGTGGAGGAGGGGAGCCGCGTGAAACAGGGCGA
AATCCTCGCCCGCCTGGAAAACCTCGATGCCGCGGCAGCCAGGGATCAGGCCGCCGCCACCGTGAAAACTTCCCGGGCGA
GCCTCGACCAGGCCAAGGCCGAGCTCACCGATGCTACCCTGGCCTTTGAGCGGGAGAAACAACTCCTGAAGGAGGGGATT
GTCGCCAAGGCCGAGTACGATTCCGCCGAGGCCCGCTACCGCCGGGCAAAGGCGGCAGTGACCGGGGCCAGTGCCTCCAT
CGTCGCTTCCGAGGCGGCCCTCAGGGGTGCCCAGGTGAATCTGGAATACTCCTTCATCCGTGCCCCCTTCGACGCCGTGG
TCCTTACCAAGAACGCCGATATCGGCGATATCGTCACCCCCATCGGCGCCGCGGCCAACGCCAAGGCCGCGGTGGTCACC
ATTGCCGACCTCGGCTCCCTCCAGGTGGAGGCCGATGTCTCCGAATCGAACCTGGAGACGGTAAAGGTCGGGCAACCGTG
CGAGATACAGCTGGATGCCCTTCCCGAAGTGCGCTTTAAGGGGAGGGTCCACATGGTGGTCCCCACCGCCGACCGCTCCA
AGGCGACAGTGCTCGTGAAGGTCCGCTTCGAGAAGCTCGACCCGCGGATACTGCCGGAGATGAGCGCCAAGGTGGCTTTT
CTGGAGCATCTCGTGGCGCCGGGCGAGGAAAAGCCCAAGACGGCGGTGAACCCCGCTGCGGTAGTGGAGCGGGGAGGGCG
GAAGGTGCTCTTTCTCGTGAAGGGTGGCCGGGTGGCGGAAACGCCAGTCACCGTGGGCGCGAAGATCGGCGAGATGCTGG
AGATAACCGCCGGCGCCAAGCCGGGCGACAAGATCGCCCTCAAGCCCCTGGATAAGCTCAAGGACGGGACGAAGATCAAG
ACGGCGGAGAAGTAG

Upstream 100 bases:

>100_bases
TGTGGCCTCTTCCCCTCTTCACACCATGGGTTACTAATGCTATGCTCTTACGTCATTGCCGCGTAGCAAACTTTCACATG
AATCCTCCCCGGAGAACTCT

Downstream 100 bases:

>100_bases
ATGGCAGAAACCACCCCTCCCATCGTTGCCGTGCGCAACCTCTCCAAGTCGTACCGGCGGGGCCAGCAGATCGTGCCGGT
GCTTCAGGATATCTCCTTCG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MANEDLGKLKIDKGALARSGGRRRPRFWIVAAVAVVVLLILFVSGVLSPAVTVETGTVTQVYPSQAYTLLNASGYVVAQR
KAAVAAKVTGRLDWLGVEEGSRVKQGEILARLENLDAAAARDQAAATVKTSRASLDQAKAELTDATLAFEREKQLLKEGI
VAKAEYDSAEARYRRAKAAVTGASASIVASEAALRGAQVNLEYSFIRAPFDAVVLTKNADIGDIVTPIGAAANAKAAVVT
IADLGSLQVEADVSESNLETVKVGQPCEIQLDALPEVRFKGRVHMVVPTADRSKATVLVKVRFEKLDPRILPEMSAKVAF
LEHLVAPGEEKPKTAVNPAAVVERGGRKVLFLVKGGRVAETPVTVGAKIGEMLEITAGAKPGDKIALKPLDKLKDGTKIK
TAEK

Sequences:

>Translated_404_residues
MANEDLGKLKIDKGALARSGGRRRPRFWIVAAVAVVVLLILFVSGVLSPAVTVETGTVTQVYPSQAYTLLNASGYVVAQR
KAAVAAKVTGRLDWLGVEEGSRVKQGEILARLENLDAAAARDQAAATVKTSRASLDQAKAELTDATLAFEREKQLLKEGI
VAKAEYDSAEARYRRAKAAVTGASASIVASEAALRGAQVNLEYSFIRAPFDAVVLTKNADIGDIVTPIGAAANAKAAVVT
IADLGSLQVEADVSESNLETVKVGQPCEIQLDALPEVRFKGRVHMVVPTADRSKATVLVKVRFEKLDPRILPEMSAKVAF
LEHLVAPGEEKPKTAVNPAAVVERGGRKVLFLVKGGRVAETPVTVGAKIGEMLEITAGAKPGDKIALKPLDKLKDGTKIK
TAEK
>Mature_403_residues
ANEDLGKLKIDKGALARSGGRRRPRFWIVAAVAVVVLLILFVSGVLSPAVTVETGTVTQVYPSQAYTLLNASGYVVAQRK
AAVAAKVTGRLDWLGVEEGSRVKQGEILARLENLDAAAARDQAAATVKTSRASLDQAKAELTDATLAFEREKQLLKEGIV
AKAEYDSAEARYRRAKAAVTGASASIVASEAALRGAQVNLEYSFIRAPFDAVVLTKNADIGDIVTPIGAAANAKAAVVTI
ADLGSLQVEADVSESNLETVKVGQPCEIQLDALPEVRFKGRVHMVVPTADRSKATVLVKVRFEKLDPRILPEMSAKVAFL
EHLVAPGEEKPKTAVNPAAVVERGGRKVLFLVKGGRVAETPVTVGAKIGEMLEITAGAKPGDKIALKPLDKLKDGTKIKT
AEK

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=363, Percent_Identity=27.8236914600551, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082036, Length=369, Percent_Identity=26.2872628726287, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789929, Length=366, Percent_Identity=24.5901639344262, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790519, Length=299, Percent_Identity=27.7591973244147, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42759; Mature: 42628

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEDLGKLKIDKGALARSGGRRRPRFWIVAAVAVVVLLILFVSGVLSPAVTVETGTVTQ
CCCCCCCCEEECCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEE
VYPSQAYTLLNASGYVVAQRKAAVAAKVTGRLDWLGVEEGSRVKQGEILARLENLDAAAA
ECCCCEEEEECCCCEEEEECCHHEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCHHHH
RDQAAATVKTSRASLDQAKAELTDATLAFEREKQLLKEGIVAKAEYDSAEARYRRAKAAV
CCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHC
TGASASIVASEAALRGAQVNLEYSFIRAPFDAVVLTKNADIGDIVTPIGAAANAKAAVVT
CCCCHHHHHHHHHHCCEEEEEEEEEEECCCEEEEEECCCCCCHHHCCCCCCCCCCEEEEE
IADLGSLQVEADVSESNLETVKVGQPCEIQLDALPEVRFKGRVHMVVPTADRSKATVLVK
EECCCCEEEEECCCCCCCCEEECCCCCEEEECCCCCEEECCEEEEEEECCCCCCEEEEEE
VRFEKLDPRILPEMSAKVAFLEHLVAPGEEKPKTAVNPAAVVERGGRKVLFLVKGGRVAE
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCEEEC
TPVTVGAKIGEMLEITAGAKPGDKIALKPLDKLKDGTKIKTAEK
CCEECCCHHCCEEEEECCCCCCCEEEECCHHHCCCCCEEECCCC
>Mature Secondary Structure 
ANEDLGKLKIDKGALARSGGRRRPRFWIVAAVAVVVLLILFVSGVLSPAVTVETGTVTQ
CCCCCCCEEECCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEE
VYPSQAYTLLNASGYVVAQRKAAVAAKVTGRLDWLGVEEGSRVKQGEILARLENLDAAAA
ECCCCEEEEECCCCEEEEECCHHEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCHHHH
RDQAAATVKTSRASLDQAKAELTDATLAFEREKQLLKEGIVAKAEYDSAEARYRRAKAAV
CCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHC
TGASASIVASEAALRGAQVNLEYSFIRAPFDAVVLTKNADIGDIVTPIGAAANAKAAVVT
CCCCHHHHHHHHHHCCEEEEEEEEEEECCCEEEEEECCCCCCHHHCCCCCCCCCCEEEEE
IADLGSLQVEADVSESNLETVKVGQPCEIQLDALPEVRFKGRVHMVVPTADRSKATVLVK
EECCCCEEEEECCCCCCCCEEECCCCCEEEECCCCCEEECCEEEEEEECCCCCCEEEEEE
VRFEKLDPRILPEMSAKVAFLEHLVAPGEEKPKTAVNPAAVVERGGRKVLFLVKGGRVAE
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCEEEC
TPVTVGAKIGEMLEITAGAKPGDKIALKPLDKLKDGTKIKTAEK
CCEECCCHHCCEEEEECCCCCCCEEEECCHHHCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]