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The map label for this gene is tgt [H]

Identifier: 78223601

GI number: 78223601

Start: 2709976

End: 2711082

Strand: Direct

Name: tgt [H]

Synonym: Gmet_2398

Alternate gene names: 78223601

Gene position: 2709976-2711082 (Clockwise)

Preceding gene: 78223595

Following gene: 78223602

Centisome position: 67.79

GC content: 57.0

Gene sequence:

>1107_bases
ATGAAATTTACCCTCAATAAAAAGGACAGGAAGACCGCTGCCCGGCGGGGGACATTGGTAACGCCGCACGGCACTATCGA
AACTCCTATCTTCATGCCGGTGGGCACCCACGCAGCCATGAAGGCAATGACACCGGTACAGGTGAAGGAGACCGGCGCCC
AGATCATCCTCTCCAACACCTATCACCTTCATCTCCGTCCTGGCGAGGAACTGGTGGCGAAAGCCGGCGGACTCCACAGC
TTCATGGCCTGGGACGGACCTATCCTGACCGACTCCGGCGGGTTCCAGGTCTTCTCGCTCCCCAACAAGCGGATAACCGA
GGAAGGCGCCTACTTCAAGCATGAGGTTTCCGGCGAGGAGGTCTTTCTCGACCCGGCCAGAGCCATCGCCATCCAGGAAG
ACCTGGGCGCCGACATAATCATGGCTTTCGACGAGTGCATTCCCTACCCCTGCGACCGCAAGTACGCGGCTAAATCGACC
CGAAAAACCCTCCGCTGGGCAGAGCAGTGCAAGAAAGCCCAGACACGGAAAGATCAGGCCCTGTTCGGCATCGTACAGGG
AAGCGTCTTCGAGGATCTGCGCGCCATGTGCGCCAAAGAACTCGTGCAGATGGATTTCCCCGGCTACGCCATCGGCGGCG
TCTCCGTCGGCGAGGGGCTGGATCTGCTAAAGAAAGTGGTGGAGTACACAGCGCCCTACCTGCCGGAAAACAAGCCCCGC
TACCTCATGGGCGTTGGACTCCCCGAGGATATCCTCGAAAGTGTCGAGCGAGGGATGGACATGTTCGACTGTGTTATCCC
AACCCGTTACGCACGCAGTGCCACCCTCTTTACAAACCGGGGAAAGATTCGCCTCACCAACCGCAAATACCGGCGCGACT
TCTACCCCATCGAGCCGAACTGCACCTGTTACACCTGCCGGAACTTCACCAGGGCATACATCCATCATCTCTTCAACGCC
AATGAAGTACTCTCCGCTATCCTTGCAAGCATTCACAACGTTCACTTCTATCTGAACATGATGGCGGAAATTCGCCGTGC
AATCGAAGAGGGACGTTTTACAGACTATAAGCGGGAATTTCTGGAATCATACCTGAAGGGCGCTTAG

Upstream 100 bases:

>100_bases
TCTGTTAACAATGCTGGCCAACCATGCAGATAAAAACAGGTCTCAACTGAGTAACCATTAATCTTCGGGCTTTGTAATCC
AATTATTCAGAAAAGGCAAC

Downstream 100 bases:

>100_bases
AGAAGAGGTTTACATTTCAGCAATCAACGGGGCATCCAGGCGGATGCCCCGTTACGTTTCACGGACGGGAGAGAGTCGCC
ATGAAAAACGAGGGTGCGGG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKFTLNKKDRKTAARRGTLVTPHGTIETPIFMPVGTHAAMKAMTPVQVKETGAQIILSNTYHLHLRPGEELVAKAGGLHS
FMAWDGPILTDSGGFQVFSLPNKRITEEGAYFKHEVSGEEVFLDPARAIAIQEDLGADIIMAFDECIPYPCDRKYAAKST
RKTLRWAEQCKKAQTRKDQALFGIVQGSVFEDLRAMCAKELVQMDFPGYAIGGVSVGEGLDLLKKVVEYTAPYLPENKPR
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTNRKYRRDFYPIEPNCTCYTCRNFTRAYIHHLFNA
NEVLSAILASIHNVHFYLNMMAEIRRAIEEGRFTDYKREFLESYLKGA

Sequences:

>Translated_368_residues
MKFTLNKKDRKTAARRGTLVTPHGTIETPIFMPVGTHAAMKAMTPVQVKETGAQIILSNTYHLHLRPGEELVAKAGGLHS
FMAWDGPILTDSGGFQVFSLPNKRITEEGAYFKHEVSGEEVFLDPARAIAIQEDLGADIIMAFDECIPYPCDRKYAAKST
RKTLRWAEQCKKAQTRKDQALFGIVQGSVFEDLRAMCAKELVQMDFPGYAIGGVSVGEGLDLLKKVVEYTAPYLPENKPR
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTNRKYRRDFYPIEPNCTCYTCRNFTRAYIHHLFNA
NEVLSAILASIHNVHFYLNMMAEIRRAIEEGRFTDYKREFLESYLKGA
>Mature_368_residues
MKFTLNKKDRKTAARRGTLVTPHGTIETPIFMPVGTHAAMKAMTPVQVKETGAQIILSNTYHLHLRPGEELVAKAGGLHS
FMAWDGPILTDSGGFQVFSLPNKRITEEGAYFKHEVSGEEVFLDPARAIAIQEDLGADIIMAFDECIPYPCDRKYAAKST
RKTLRWAEQCKKAQTRKDQALFGIVQGSVFEDLRAMCAKELVQMDFPGYAIGGVSVGEGLDLLKKVVEYTAPYLPENKPR
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTNRKYRRDFYPIEPNCTCYTCRNFTRAYIHHLFNA
NEVLSAILASIHNVHFYLNMMAEIRRAIEEGRFTDYKREFLESYLKGA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=353, Percent_Identity=43.342776203966, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI13375872, Length=355, Percent_Identity=24.7887323943662, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=52.7173913043478, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17544680, Length=357, Percent_Identity=43.6974789915966, Blast_Score=307, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI25146502, Length=216, Percent_Identity=26.8518518518519, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920510, Length=358, Percent_Identity=41.6201117318436, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI21355311, Length=158, Percent_Identity=34.1772151898734, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41677; Mature: 41677

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLNKKDRKTAARRGTLVTPHGTIETPIFMPVGTHAAMKAMTPVQVKETGAQIILSNT
CCCCCCCHHHHHHHHCCCEECCCCCCCCCEEEECCCHHHHHHCCCCEECCCCCEEEEECE
YHLHLRPGEELVAKAGGLHSFMAWDGPILTDSGGFQVFSLPNKRITEEGAYFKHEVSGEE
EEEEECCCHHHHHHHCCCHHHHHCCCCEEECCCCEEEEECCCCHHCCCCCCEEEECCCCE
VFLDPARAIAIQEDLGADIIMAFDECIPYPCDRKYAAKSTRKTLRWAEQCKKAQTRKDQA
EEECHHHHEEEHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGIVQGSVFEDLRAMCAKELVQMDFPGYAIGGVSVGEGLDLLKKVVEYTAPYLPENKPR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTNRKYRRDFYPIEPN
EEEECCCCHHHHHHHHHCCHHHHHHCCHHHCCCEEEEECCCEEEECCHHHHCCCCCCCCC
CTCYTCRNFTRAYIHHLFNANEVLSAILASIHNVHFYLNMMAEIRRAIEEGRFTDYKREF
CEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LESYLKGA
HHHHHCCC
>Mature Secondary Structure
MKFTLNKKDRKTAARRGTLVTPHGTIETPIFMPVGTHAAMKAMTPVQVKETGAQIILSNT
CCCCCCCHHHHHHHHCCCEECCCCCCCCCEEEECCCHHHHHHCCCCEECCCCCEEEEECE
YHLHLRPGEELVAKAGGLHSFMAWDGPILTDSGGFQVFSLPNKRITEEGAYFKHEVSGEE
EEEEECCCHHHHHHHCCCHHHHHCCCCEEECCCCEEEEECCCCHHCCCCCCEEEECCCCE
VFLDPARAIAIQEDLGADIIMAFDECIPYPCDRKYAAKSTRKTLRWAEQCKKAQTRKDQA
EEECHHHHEEEHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGIVQGSVFEDLRAMCAKELVQMDFPGYAIGGVSVGEGLDLLKKVVEYTAPYLPENKPR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTNRKYRRDFYPIEPN
EEEECCCCHHHHHHHHHCCHHHHHHCCHHHCCCEEEEECCCEEEECCHHHHCCCCCCCCC
CTCYTCRNFTRAYIHHLFNANEVLSAILASIHNVHFYLNMMAEIRRAIEEGRFTDYKREF
CEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LESYLKGA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA