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The map label for this gene is mdeA [H]

Identifier: 78223593

GI number: 78223593

Start: 2699868

End: 2701154

Strand: Reverse

Name: mdeA [H]

Synonym: Gmet_2390

Alternate gene names: 78223593

Gene position: 2701154-2699868 (Counterclockwise)

Preceding gene: 78223594

Following gene: 78223592

Centisome position: 67.57

GC content: 60.92

Gene sequence:

>1287_bases
ATGACCGATACGAAAATAAGCTTCGACACCCTCGCCCTGCACGCCGGCCAGGAACCCGACCCCACAACCCTTTCCCGGGC
GGTTCCCATCTACCAGACCGCGTCTTACGCCTTCAAGAGTTCAGAGCATGCAGCCAACCTCTTCGGGCTCAAGGAGTTCG
GCAACATCTATACTCGGCTCATGAATCCCACCAGTGACGTGCTGGAAAAGCGCCTTGCCGAACTGGACGGTGGGGTTGGA
GCACTTGCCGTCGCCTCGGGCCAGGCGGCCATCACCTACGCCGTGCTCAATATTGCCCAGGCGGGGCAGAACATCGTCTC
CACCAGCTATCTCTACGGGGGGACCTACAACCTCTTCCATTACACCCTCCCCAAACTGGGGATTACCGTGAAGTTCGTCG
ATACCTCCGACCCGGAGAACGTCCGGCGGGCCATCGATGACAAGACCCGCCTCGTCTACACCGAGTCGGTGGGGAACCCC
AAGAACAACGTGGACGACTTCGAGGCCATTGCCCGCATCGCCCACGAAGCCGGCATCCCCTTCGTGGTCGACAATACCGT
GACCACGTCATACCTGTTCAAGCCCCTGGAGCACGGTGCCGACATCGTTGTCTACTCCCTCACCAAGTTCATTGGCGGCC
ACGGCACGAGCATCGGCGGCGCAGTGGTGGACGGCGGGAAGTTCCCCTGGAATAACGGCCGGTTCCCCGAAATGACTGAG
CCTGATCCCTCCTACCACGGCCTCTGCTACTGGGAAGCCCTCGGGAATCTCTCCTACATCCTCAAGATGAGGATCACGCT
CCTGCGCGATATGGGAGCCTGCATCTCTCCGTTCAACTCTTTTCTCTTCCTGCAGGGGCTGGAAACCCTCCCGGTACGGA
TGGCCCGCCACGTGGAGAACGCCCGGGCGGTTGCCCAATGGCTCGAAAAGCATCCCCTTGTCTCCTGGGTGAATTATCCC
GGCCTTGCGAGCCACAAAGACCACGGCAACGCCAGGAAGTACCTGCCGAAGGGGGAAGGCGCCATTATCGGCTTCGGCAT
CAACGGGGGGCTCGAGGCGGGAAGACGGTTCATCGACAGCGCGAAGCTGCTCTCCCACCTGGCGAACATCGGTGACGCCA
AGTCGTTGGTGATCCACCCCGCCTCCACCACCCACCAGCAACTCTCCGAGGAGGAACAGCTGGCGAGCGGCGTCACCCAG
GATTATATCCGCCTCTCCATCGGCATTGAGGAGGTGGCTGATATCATTGCCGACATCGACCAGGCATTGGCGGCGTCCCA
AGGGTAA

Upstream 100 bases:

>100_bases
ATGTTCTTGACAGACGCGGTAGGTATTAACTACATTTTAAGCCACTGTATATAACAATAAAGGTGGGTTATATTTCTTAA
CCCCGGGAGAAGATGCATCA

Downstream 100 bases:

>100_bases
CTCCATCCACCCTTTGGGGTGTTGAATTGGCCACCGGGAAAAGGGCGATCCTCAAAGATCGCCTTTTTTCATTTGCATCA
ACCGGGGGGCAGCGGTACAC

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTDTKISFDTLALHAGQEPDPTTLSRAVPIYQTASYAFKSSEHAANLFGLKEFGNIYTRLMNPTSDVLEKRLAELDGGVG
ALAVASGQAAITYAVLNIAQAGQNIVSTSYLYGGTYNLFHYTLPKLGITVKFVDTSDPENVRRAIDDKTRLVYTESVGNP
KNNVDDFEAIARIAHEAGIPFVVDNTVTTSYLFKPLEHGADIVVYSLTKFIGGHGTSIGGAVVDGGKFPWNNGRFPEMTE
PDPSYHGLCYWEALGNLSYILKMRITLLRDMGACISPFNSFLFLQGLETLPVRMARHVENARAVAQWLEKHPLVSWVNYP
GLASHKDHGNARKYLPKGEGAIIGFGINGGLEAGRRFIDSAKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVTQ
DYIRLSIGIEEVADIIADIDQALAASQG

Sequences:

>Translated_428_residues
MTDTKISFDTLALHAGQEPDPTTLSRAVPIYQTASYAFKSSEHAANLFGLKEFGNIYTRLMNPTSDVLEKRLAELDGGVG
ALAVASGQAAITYAVLNIAQAGQNIVSTSYLYGGTYNLFHYTLPKLGITVKFVDTSDPENVRRAIDDKTRLVYTESVGNP
KNNVDDFEAIARIAHEAGIPFVVDNTVTTSYLFKPLEHGADIVVYSLTKFIGGHGTSIGGAVVDGGKFPWNNGRFPEMTE
PDPSYHGLCYWEALGNLSYILKMRITLLRDMGACISPFNSFLFLQGLETLPVRMARHVENARAVAQWLEKHPLVSWVNYP
GLASHKDHGNARKYLPKGEGAIIGFGINGGLEAGRRFIDSAKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVTQ
DYIRLSIGIEEVADIIADIDQALAASQG
>Mature_427_residues
TDTKISFDTLALHAGQEPDPTTLSRAVPIYQTASYAFKSSEHAANLFGLKEFGNIYTRLMNPTSDVLEKRLAELDGGVGA
LAVASGQAAITYAVLNIAQAGQNIVSTSYLYGGTYNLFHYTLPKLGITVKFVDTSDPENVRRAIDDKTRLVYTESVGNPK
NNVDDFEAIARIAHEAGIPFVVDNTVTTSYLFKPLEHGADIVVYSLTKFIGGHGTSIGGAVVDGGKFPWNNGRFPEMTEP
DPSYHGLCYWEALGNLSYILKMRITLLRDMGACISPFNSFLFLQGLETLPVRMARHVENARAVAQWLEKHPLVSWVNYPG
LASHKDHGNARKYLPKGEGAIIGFGINGGLEAGRRFIDSAKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVTQD
YIRLSIGIEEVADIIADIDQALAASQG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=421, Percent_Identity=35.1543942992874, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=32.0665083135392, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI25453487, Length=420, Percent_Identity=30.7142857142857, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1790375, Length=418, Percent_Identity=31.3397129186603, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=27.2093023255814, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538055, Length=419, Percent_Identity=34.6062052505967, Blast_Score=207, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI17560056, Length=419, Percent_Identity=35.799522673031, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71980708, Length=406, Percent_Identity=31.0344827586207, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=54.292343387471, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319307, Length=424, Percent_Identity=33.7264150943396, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=441, Percent_Identity=26.3038548752834, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=199, Percent_Identity=30.1507537688442, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6321904, Length=433, Percent_Identity=23.7875288683603, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=33.0166270783848, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46485; Mature: 46354

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTKISFDTLALHAGQEPDPTTLSRAVPIYQTASYAFKSSEHAANLFGLKEFGNIYTRL
CCCCEEEHHHEEEECCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MNPTSDVLEKRLAELDGGVGALAVASGQAAITYAVLNIAQAGQNIVSTSYLYGGTYNLFH
CCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHEEECCEEHHHH
YTLPKLGITVKFVDTSDPENVRRAIDDKTRLVYTESVGNPKNNVDDFEAIARIAHEAGIP
EECCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
FVVDNTVTTSYLFKPLEHGADIVVYSLTKFIGGHGTSIGGAVVDGGKFPWNNGRFPEMTE
EEECCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCC
PDPSYHGLCYWEALGNLSYILKMRITLLRDMGACISPFNSFLFLQGLETLPVRMARHVEN
CCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
ARAVAQWLEKHPLVSWVNYPGLASHKDHGNARKYLPKGEGAIIGFGINGGLEAGRRFIDS
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHH
AKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVTQDYIRLSIGIEEVADIIADID
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHEECCHHHHHHHHHHHH
QALAASQG
HHHHCCCC
>Mature Secondary Structure 
TDTKISFDTLALHAGQEPDPTTLSRAVPIYQTASYAFKSSEHAANLFGLKEFGNIYTRL
CCCEEEHHHEEEECCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MNPTSDVLEKRLAELDGGVGALAVASGQAAITYAVLNIAQAGQNIVSTSYLYGGTYNLFH
CCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHEEECCEEHHHH
YTLPKLGITVKFVDTSDPENVRRAIDDKTRLVYTESVGNPKNNVDDFEAIARIAHEAGIP
EECCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
FVVDNTVTTSYLFKPLEHGADIVVYSLTKFIGGHGTSIGGAVVDGGKFPWNNGRFPEMTE
EEECCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCC
PDPSYHGLCYWEALGNLSYILKMRITLLRDMGACISPFNSFLFLQGLETLPVRMARHVEN
CCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
ARAVAQWLEKHPLVSWVNYPGLASHKDHGNARKYLPKGEGAIIGFGINGGLEAGRRFIDS
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHH
AKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVTQDYIRLSIGIEEVADIIADID
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHEECCHHHHHHHHHHHH
QALAASQG
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]