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The map label for this gene is acsA [H]

Identifier: 78223543

GI number: 78223543

Start: 2646808

End: 2648427

Strand: Reverse

Name: acsA [H]

Synonym: Gmet_2340

Alternate gene names: 78223543

Gene position: 2648427-2646808 (Counterclockwise)

Preceding gene: 78223544

Following gene: 78223542

Centisome position: 66.25

GC content: 59.75

Gene sequence:

>1620_bases
ATGCTGATAGAAAACGGCCAGTACAACATCGGGGATATCTGTACCTCCCGCCAATGCCGACTGGGGAGGGGAGATCGCGT
TGCCATGCGGTGGCTCACGCCCGGCATGGAGCGTCGTGATTACACCTTCAACGAGCTTGACGGCCTTTCGGGCAGATTCG
CCAATGTTCTCGCCTCCCTGGGGGTCGGGGCTGGCGAGGTGTTCTTCACGTACCTGCCGAAGCAGCCGGAACAGTTCGTT
GCCTTTCTTGGCGCATTGAAACTTACCGCGGTTGCAGGCACCCTTTTCTCCAACTTCGGCGAAGAGGCTCTCCTGGACCG
TCTCGCCGACGCCCGTGCGTGCGGCATCATCACCAAGAAGAGCCTTCTCCGGAAAATTATCCGAATCCGCCCGCAACTGC
CGGATCTTCGCTTCATCATCCTGGTTGACAGCGACGATCACCAGGCCGACGGCATTTTCAGTTACAGCCGGCTCATGGCG
GAGGCATCCCCCGCGTTCGTCACTCCCCTGACACCTGCGGATGCGCCGTCGGTTCTCCATTACACCTCTGGGTCCACGGG
AAAGCCGAAGGGGGTGCTCCATCGCCACGGCGCGGTGACTATGATCAACTCGACTGCCCAGCTGGTCCTCGGGCTTCGTG
AGGATGATGTGTACTGGTGCACCGCTGATCAGGGATGGGTAACCGGAACCTCCTACGGCATTATCGGCCCCTGGAGCATG
GGCGTGACCCAGGTTCACTTCGGCGGCGGCTATGCAGCAGAGCAGTGGTTCGAGATCCTGGAGCGGGAAGAGGTTTCGGT
CTGGTACACTGCGCCGACTGCCCTGCGAATGCTCATGAGGGAGGAACCGCTCCTTTTCGCCCGCTTTGACCTCGGCATGC
TCCGGCATATTTGCAGTGTTGGCGAGCCGCTCAACCCGGAGGTGATCCACTGGGCCCGCCGCGTTCTGGCAAAAGAGGTT
TATGATACCTGGTTCCAGACCGAAACCGGCGCCATTATGATTGCCAACCGTCCGGGAATCCCGGTGAAACCGGGTTCCAT
GGGGAAGCCGGTGGATGGCATCGAGGCGGTTGTCATATCCACCGAGGGGGAACTCCTCGGCTCCGGCCAACAGGGGGACC
TGTGCCTCAAGCCGGGATGGGCTTCCATGTTCTCCTCTTACCTGAACAACGCCGGTGCCTATGCATCAAAGTTCAGGAAC
GGCTTCTACTGCACGGGTGATGTCGCCTATCGCGATACCGACGGATATTTCTGGTTCGTCGGCAGGAGCGACGATGTTAT
CAATACGGCTGGGCATCTCATCAGCCCCTTCGAGATCGAGAGCGCGCTCATCGAGGTCCACGAGGTGGCTGAGGCAGGGG
TGATCGGTGCCCCCGACGAACTGCTCTACGAAAAGGTCGTGGCCTTTGTATCCCTCCACCTGGGGTATGCCTTGACCGAC
GAACTCCAGTTGAAGATTCGGCTTCACCTGGCCAACAAGCTTTCTTCGGTTGCAACCCCCCACGAGATCCACTGCCTCGA
ACGGATTCCCAAGAACAAGAGCGGCAAGATCATGCGCCGCTACCTGAAGGCAACCTACCTGGGGCAGGACGCCGGGGACA
TCTCTACCATGGAGGAATGA

Upstream 100 bases:

>100_bases
ATTGCCTGCATGGACGCCATCACCCCTGAAGAGGTGATGACCGAACTGGCTGGATTGCTGGAAACGGGAGGTCGACCATG
AGCAGTTTTGCGGGGAAAGC

Downstream 100 bases:

>100_bases
ACTATGCCGACCCTGGACGCACTCACCCCGATCTTCCGCCAGGTTTTCGACGACGACAGCATCGTCCTGACCCGCGAGAC
TTCGGCCAATGACATCGATG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acetyl-CoA synthase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLIENGQYNIGDICTSRQCRLGRGDRVAMRWLTPGMERRDYTFNELDGLSGRFANVLASLGVGAGEVFFTYLPKQPEQFV
AFLGALKLTAVAGTLFSNFGEEALLDRLADARACGIITKKSLLRKIIRIRPQLPDLRFIILVDSDDHQADGIFSYSRLMA
EASPAFVTPLTPADAPSVLHYTSGSTGKPKGVLHRHGAVTMINSTAQLVLGLREDDVYWCTADQGWVTGTSYGIIGPWSM
GVTQVHFGGGYAAEQWFEILEREEVSVWYTAPTALRMLMREEPLLFARFDLGMLRHICSVGEPLNPEVIHWARRVLAKEV
YDTWFQTETGAIMIANRPGIPVKPGSMGKPVDGIEAVVISTEGELLGSGQQGDLCLKPGWASMFSSYLNNAGAYASKFRN
GFYCTGDVAYRDTDGYFWFVGRSDDVINTAGHLISPFEIESALIEVHEVAEAGVIGAPDELLYEKVVAFVSLHLGYALTD
ELQLKIRLHLANKLSSVATPHEIHCLERIPKNKSGKIMRRYLKATYLGQDAGDISTMEE

Sequences:

>Translated_539_residues
MLIENGQYNIGDICTSRQCRLGRGDRVAMRWLTPGMERRDYTFNELDGLSGRFANVLASLGVGAGEVFFTYLPKQPEQFV
AFLGALKLTAVAGTLFSNFGEEALLDRLADARACGIITKKSLLRKIIRIRPQLPDLRFIILVDSDDHQADGIFSYSRLMA
EASPAFVTPLTPADAPSVLHYTSGSTGKPKGVLHRHGAVTMINSTAQLVLGLREDDVYWCTADQGWVTGTSYGIIGPWSM
GVTQVHFGGGYAAEQWFEILEREEVSVWYTAPTALRMLMREEPLLFARFDLGMLRHICSVGEPLNPEVIHWARRVLAKEV
YDTWFQTETGAIMIANRPGIPVKPGSMGKPVDGIEAVVISTEGELLGSGQQGDLCLKPGWASMFSSYLNNAGAYASKFRN
GFYCTGDVAYRDTDGYFWFVGRSDDVINTAGHLISPFEIESALIEVHEVAEAGVIGAPDELLYEKVVAFVSLHLGYALTD
ELQLKIRLHLANKLSSVATPHEIHCLERIPKNKSGKIMRRYLKATYLGQDAGDISTMEE
>Mature_539_residues
MLIENGQYNIGDICTSRQCRLGRGDRVAMRWLTPGMERRDYTFNELDGLSGRFANVLASLGVGAGEVFFTYLPKQPEQFV
AFLGALKLTAVAGTLFSNFGEEALLDRLADARACGIITKKSLLRKIIRIRPQLPDLRFIILVDSDDHQADGIFSYSRLMA
EASPAFVTPLTPADAPSVLHYTSGSTGKPKGVLHRHGAVTMINSTAQLVLGLREDDVYWCTADQGWVTGTSYGIIGPWSM
GVTQVHFGGGYAAEQWFEILEREEVSVWYTAPTALRMLMREEPLLFARFDLGMLRHICSVGEPLNPEVIHWARRVLAKEV
YDTWFQTETGAIMIANRPGIPVKPGSMGKPVDGIEAVVISTEGELLGSGQQGDLCLKPGWASMFSSYLNNAGAYASKFRN
GFYCTGDVAYRDTDGYFWFVGRSDDVINTAGHLISPFEIESALIEVHEVAEAGVIGAPDELLYEKVVAFVSLHLGYALTD
ELQLKIRLHLANKLSSVATPHEIHCLERIPKNKSGKIMRRYLKATYLGQDAGDISTMEE

Specific function: Has a role in growth and sporulation on acetate [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI8923896, Length=573, Percent_Identity=34.5549738219895, Blast_Score=279, Evalue=5e-75,
Organism=Homo sapiens, GI28416953, Length=564, Percent_Identity=32.4468085106383, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI253970489, Length=585, Percent_Identity=33.5042735042735, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI42544132, Length=527, Percent_Identity=30.1707779886148, Blast_Score=256, Evalue=5e-68,
Organism=Homo sapiens, GI122937307, Length=506, Percent_Identity=31.4229249011858, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI38505220, Length=533, Percent_Identity=30.0187617260788, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=29.0944123314065, Blast_Score=222, Evalue=7e-58,
Organism=Homo sapiens, GI58082049, Length=507, Percent_Identity=28.4023668639053, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI13375727, Length=578, Percent_Identity=28.2006920415225, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI157311624, Length=507, Percent_Identity=27.810650887574, Blast_Score=206, Evalue=6e-53,
Organism=Homo sapiens, GI157311622, Length=507, Percent_Identity=27.810650887574, Blast_Score=206, Evalue=6e-53,
Organism=Homo sapiens, GI42544134, Length=337, Percent_Identity=26.7062314540059, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI195947378, Length=419, Percent_Identity=25.0596658711217, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI187761345, Length=379, Percent_Identity=27.4406332453826, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI187761343, Length=379, Percent_Identity=27.4406332453826, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI31982927, Length=551, Percent_Identity=22.5045372050817, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI156151445, Length=415, Percent_Identity=24.8192771084337, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1790505, Length=569, Percent_Identity=34.2706502636204, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1786529, Length=536, Percent_Identity=29.4776119402985, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI221142682, Length=506, Percent_Identity=25.6916996047431, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI145693145, Length=507, Percent_Identity=23.2741617357002, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=23.1968810916179, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1788107, Length=525, Percent_Identity=24.5714285714286, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71983001, Length=579, Percent_Identity=32.2970639032815, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI71982997, Length=579, Percent_Identity=32.2970639032815, Blast_Score=268, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI17569507, Length=557, Percent_Identity=23.5188509874327, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=355, Percent_Identity=26.7605633802817, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=517, Percent_Identity=24.5647969052224, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=353, Percent_Identity=27.1954674220963, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=541, Percent_Identity=23.2902033271719, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=22.8915662650602, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=353, Percent_Identity=24.6458923512748, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=353, Percent_Identity=24.6458923512748, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=353, Percent_Identity=24.6458923512748, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=351, Percent_Identity=24.7863247863248, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=22.0670391061453, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323182, Length=582, Percent_Identity=32.6460481099656, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6319264, Length=573, Percent_Identity=33.3333333333333, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6319699, Length=366, Percent_Identity=27.0491803278689, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24667955, Length=585, Percent_Identity=33.5042735042735, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI62472339, Length=554, Percent_Identity=33.3935018050542, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24667959, Length=516, Percent_Identity=33.3333333333333, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI21355225, Length=558, Percent_Identity=28.494623655914, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24581924, Length=417, Percent_Identity=27.0983213429257, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355181, Length=522, Percent_Identity=23.5632183908046, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356441, Length=352, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648260, Length=375, Percent_Identity=26.1333333333333, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21356947, Length=388, Percent_Identity=23.7113402061856, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI18859661, Length=518, Percent_Identity=23.9382239382239, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20130357, Length=363, Percent_Identity=25.068870523416, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648257, Length=354, Percent_Identity=25.9887005649718, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648253, Length=302, Percent_Identity=26.8211920529801, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648255, Length=302, Percent_Identity=26.8211920529801, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24656500, Length=408, Percent_Identity=24.2647058823529, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21358303, Length=308, Percent_Identity=25.974025974026, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653035, Length=352, Percent_Identity=23.0113636363636, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI161076582, Length=357, Percent_Identity=22.96918767507, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922652, Length=505, Percent_Identity=20.7920792079208, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 59490; Mature: 59490

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIENGQYNIGDICTSRQCRLGRGDRVAMRWLTPGMERRDYTFNELDGLSGRFANVLASL
CEECCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHH
GVGAGEVFFTYLPKQPEQFVAFLGALKLTAVAGTLFSNFGEEALLDRLADARACGIITKK
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SLLRKIIRIRPQLPDLRFIILVDSDDHQADGIFSYSRLMAEASPAFVTPLTPADAPSVLH
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEE
YTSGSTGKPKGVLHRHGAVTMINSTAQLVLGLREDDVYWCTADQGWVTGTSYGIIGPWSM
ECCCCCCCCCCCEECCCCEEEECCCEEEEEEECCCCEEEEECCCCEEECCCCCEECCCCC
GVTQVHFGGGYAAEQWFEILEREEVSVWYTAPTALRMLMREEPLLFARFDLGMLRHICSV
CEEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCEEEEHHHHHHHHHHHC
GEPLNPEVIHWARRVLAKEVYDTWFQTETGAIMIANRPGIPVKPGSMGKPVDGIEAVVIS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEE
TEGELLGSGQQGDLCLKPGWASMFSSYLNNAGAYASKFRNGFYCTGDVAYRDTDGYFWFV
CCCCEECCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHCCCEEEECCEEEEECCCEEEEE
GRSDDVINTAGHLISPFEIESALIEVHEVAEAGVIGAPDELLYEKVVAFVSLHLGYALTD
ECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCEEECC
ELQLKIRLHLANKLSSVATPHEIHCLERIPKNKSGKIMRRYLKATYLGQDAGDISTMEE
CEEEEEEEEEHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLIENGQYNIGDICTSRQCRLGRGDRVAMRWLTPGMERRDYTFNELDGLSGRFANVLASL
CEECCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHH
GVGAGEVFFTYLPKQPEQFVAFLGALKLTAVAGTLFSNFGEEALLDRLADARACGIITKK
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SLLRKIIRIRPQLPDLRFIILVDSDDHQADGIFSYSRLMAEASPAFVTPLTPADAPSVLH
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEE
YTSGSTGKPKGVLHRHGAVTMINSTAQLVLGLREDDVYWCTADQGWVTGTSYGIIGPWSM
ECCCCCCCCCCCEECCCCEEEECCCEEEEEEECCCCEEEEECCCCEEECCCCCEECCCCC
GVTQVHFGGGYAAEQWFEILEREEVSVWYTAPTALRMLMREEPLLFARFDLGMLRHICSV
CEEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCEEEEHHHHHHHHHHHC
GEPLNPEVIHWARRVLAKEVYDTWFQTETGAIMIANRPGIPVKPGSMGKPVDGIEAVVIS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEE
TEGELLGSGQQGDLCLKPGWASMFSSYLNNAGAYASKFRNGFYCTGDVAYRDTDGYFWFV
CCCCEECCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHCCCEEEECCEEEEECCCEEEEE
GRSDDVINTAGHLISPFEIESALIEVHEVAEAGVIGAPDELLYEKVVAFVSLHLGYALTD
ECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCEEECC
ELQLKIRLHLANKLSSVATPHEIHCLERIPKNKSGKIMRRYLKATYLGQDAGDISTMEE
CEEEEEEEEEHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934817; 9387221; 9384377 [H]