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The map label for this gene is cca [H]

Identifier: 78223488

GI number: 78223488

Start: 2590079

End: 2591374

Strand: Reverse

Name: cca [H]

Synonym: Gmet_2285

Alternate gene names: 78223488

Gene position: 2591374-2590079 (Counterclockwise)

Preceding gene: 78223489

Following gene: 78223486

Centisome position: 64.83

GC content: 57.87

Gene sequence:

>1296_bases
ATGGACGCTTCCATCGCATCTTTCCTGCAAAATCCGTTTCTGAGAACGCTGCGGCAGGCGTCAGCAAGCCTTGATCTACA
ATGCTATGTTGTTGGAGGATGCCTGCGCGACCTGCTGATGGGGCGCGGGGTGAACGACGTGGATATTGCCGTCGGCGGCG
GGCCTGAGGCTCTTTCCCGCCGCTTTGCCAAAGAGTGTGGCGGCACCTTCTTCTGGCTCGACCAGGAGCGGGGGCATTCC
CGCGTCGTCATCAAGGGAGGGGCCGGGATCGACACTTTCGACTTTGCTCCCCTGAGAGGTGAGGATATCTGTGCTGATCT
CGCCCTTCGCGACTTCACCATCAACTCTCTTGCCGTGCCTCTCCAGGGTGGGGTGGAGCTTTTCGATCCCTTAAAAGGCG
AAACCGATATCCGTGCTCAACTGGTAAGGCGTTGCGCCGAATCGGTTTTTCGTGATGACCCCCTGCGCCTTGTACGCGCA
TTCCGTTTTGCCGCCACCCTCGGCTTTCGCATCGAGACTGAAACTCTCGCGGCAATTACGACCCATGCACCTCTTATTGC
AAACAGCGCTGGGGAGCGGATTCGAGACGAACTTTTCCAGGTTCTGTGCATGCCCGGAACCGGGTCTGTTTTCCGAACTA
TGGGTGATGCGGGACTTCTCTGTGTGCTCTTCGGACTCTCTCCCTCGCAGGTTTATCCGGCAGCGGAGGCCATGGACAGG
GTCGAAGCTGTTGTGCAATCCCTTTCACAGCTGGGCGACGAAACTGCGGAAAAGGGATGCTTACGGCTTCAGGAGCAAAT
CCAGGGAGGTATGACGGTTCTGTCCCTCACGAAGCTGGCTGCTTTTCTTACTGTAACAGAAGCGGATCTCCCTGCGGCGG
CAGATCGGCTTAAGCTTGGAAAGACGGCAGGGCAACTCGTGGAGAAGCTGTGTCGTGCCGATACTCTTGCCCTTGCAGAG
CGGTTGGACACGGTCTCCGCGTACACGCTTTTCAACGCATGCGATCCCGCAGGGCTCGAACTGCCGTTGCTGCTTCTTGC
CCGAGGACACATCACCGAATCGCGCTGTCGCGAACTTGCCCGTTACTACCTGCACTGCCATATCCCCCGGGGAGGGAACC
TTCTCCTCTCTGGAGCTGAAATCATGGAGTTGCTTTCCGTTCCTCCCTGCAAGATGGTTGGGGACGCCCATGAGCTTCTC
CGGGAGGCCCAGTGCACCGGCCAAGTGAGAACTGGCGCTGAAGCAAGCGTCTTCCTCAGGAAAAAACTGTTGACAACCAA
CGAGCCCATGGGCTAA

Upstream 100 bases:

>100_bases
GGGGTCGCTGTCTGACCGCCTATTGGAGATATGTCTTGAAAAGCCCGCCCTGTGCGGGCTTTTTTCTTGCCTCGTCTCCG
CGGGGTGGTAATCTCATGCC

Downstream 100 bases:

>100_bases
AAAGTTGTACTCAATCAAGCGGGAATAACTCAGTGGTAGAGTGTCAGCTTCCCAAGCTGAAGGTCGCGGGTTCAAATCCC
GTTTCCCGCTCCATAAAGAA

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MDASIASFLQNPFLRTLRQASASLDLQCYVVGGCLRDLLMGRGVNDVDIAVGGGPEALSRRFAKECGGTFFWLDQERGHS
RVVIKGGAGIDTFDFAPLRGEDICADLALRDFTINSLAVPLQGGVELFDPLKGETDIRAQLVRRCAESVFRDDPLRLVRA
FRFAATLGFRIETETLAAITTHAPLIANSAGERIRDELFQVLCMPGTGSVFRTMGDAGLLCVLFGLSPSQVYPAAEAMDR
VEAVVQSLSQLGDETAEKGCLRLQEQIQGGMTVLSLTKLAAFLTVTEADLPAAADRLKLGKTAGQLVEKLCRADTLALAE
RLDTVSAYTLFNACDPAGLELPLLLLARGHITESRCRELARYYLHCHIPRGGNLLLSGAEIMELLSVPPCKMVGDAHELL
REAQCTGQVRTGAEASVFLRKKLLTTNEPMG

Sequences:

>Translated_431_residues
MDASIASFLQNPFLRTLRQASASLDLQCYVVGGCLRDLLMGRGVNDVDIAVGGGPEALSRRFAKECGGTFFWLDQERGHS
RVVIKGGAGIDTFDFAPLRGEDICADLALRDFTINSLAVPLQGGVELFDPLKGETDIRAQLVRRCAESVFRDDPLRLVRA
FRFAATLGFRIETETLAAITTHAPLIANSAGERIRDELFQVLCMPGTGSVFRTMGDAGLLCVLFGLSPSQVYPAAEAMDR
VEAVVQSLSQLGDETAEKGCLRLQEQIQGGMTVLSLTKLAAFLTVTEADLPAAADRLKLGKTAGQLVEKLCRADTLALAE
RLDTVSAYTLFNACDPAGLELPLLLLARGHITESRCRELARYYLHCHIPRGGNLLLSGAEIMELLSVPPCKMVGDAHELL
REAQCTGQVRTGAEASVFLRKKLLTTNEPMG
>Mature_431_residues
MDASIASFLQNPFLRTLRQASASLDLQCYVVGGCLRDLLMGRGVNDVDIAVGGGPEALSRRFAKECGGTFFWLDQERGHS
RVVIKGGAGIDTFDFAPLRGEDICADLALRDFTINSLAVPLQGGVELFDPLKGETDIRAQLVRRCAESVFRDDPLRLVRA
FRFAATLGFRIETETLAAITTHAPLIANSAGERIRDELFQVLCMPGTGSVFRTMGDAGLLCVLFGLSPSQVYPAAEAMDR
VEAVVQSLSQLGDETAEKGCLRLQEQIQGGMTVLSLTKLAAFLTVTEADLPAAADRLKLGKTAGQLVEKLCRADTLALAE
RLDTVSAYTLFNACDPAGLELPLLLLARGHITESRCRELARYYLHCHIPRGGNLLLSGAEIMELLSVPPCKMVGDAHELL
REAQCTGQVRTGAEASVFLRKKLLTTNEPMG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=210, Percent_Identity=34.2857142857143, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46600; Mature: 46600

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASIASFLQNPFLRTLRQASASLDLQCYVVGGCLRDLLMGRGVNDVDIAVGGGPEALSR
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
RFAKECGGTFFWLDQERGHSRVVIKGGAGIDTFDFAPLRGEDICADLALRDFTINSLAVP
HHHHHHCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHEECCCEEEC
LQGGVELFDPLKGETDIRAQLVRRCAESVFRDDPLRLVRAFRFAATLGFRIETETLAAIT
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEHHHHHHHH
THAPLIANSAGERIRDELFQVLCMPGTGSVFRTMGDAGLLCVLFGLSPSQVYPAAEAMDR
HCCCHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHEECCCHHHCCCHHHHHHH
VEAVVQSLSQLGDETAEKGCLRLQEQIQGGMTVLSLTKLAAFLTVTEADLPAAADRLKLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KTAGQLVEKLCRADTLALAERLDTVSAYTLFNACDPAGLELPLLLLARGHITESRCRELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHH
RYYLHCHIPRGGNLLLSGAEIMELLSVPPCKMVGDAHELLREAQCTGQVRTGAEASVFLR
HHHHEEECCCCCCEEECHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHH
KKLLTTNEPMG
HHHHCCCCCCC
>Mature Secondary Structure
MDASIASFLQNPFLRTLRQASASLDLQCYVVGGCLRDLLMGRGVNDVDIAVGGGPEALSR
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
RFAKECGGTFFWLDQERGHSRVVIKGGAGIDTFDFAPLRGEDICADLALRDFTINSLAVP
HHHHHHCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHEECCCEEEC
LQGGVELFDPLKGETDIRAQLVRRCAESVFRDDPLRLVRAFRFAATLGFRIETETLAAIT
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEHHHHHHHH
THAPLIANSAGERIRDELFQVLCMPGTGSVFRTMGDAGLLCVLFGLSPSQVYPAAEAMDR
HCCCHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHEECCCHHHCCCHHHHHHH
VEAVVQSLSQLGDETAEKGCLRLQEQIQGGMTVLSLTKLAAFLTVTEADLPAAADRLKLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KTAGQLVEKLCRADTLALAERLDTVSAYTLFNACDPAGLELPLLLLARGHITESRCRELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHH
RYYLHCHIPRGGNLLLSGAEIMELLSVPPCKMVGDAHELLREAQCTGQVRTGAEASVFLR
HHHHEEECCCCCCEEECHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHH
KKLLTTNEPMG
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA