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The map label for this gene is fadH [C]

Identifier: 78223398

GI number: 78223398

Start: 2479886

End: 2481823

Strand: Reverse

Name: fadH [C]

Synonym: Gmet_2193

Alternate gene names: 78223398

Gene position: 2481823-2479886 (Counterclockwise)

Preceding gene: 78223399

Following gene: 78223396

Centisome position: 62.09

GC content: 55.21

Gene sequence:

>1938_bases
ATGAACACAAAACCAGTCCCGGCAGAAGCGCCAGATCTCTTTTCTCCAATTACCATCAGCGGGCTGACCATAAAGAACCG
GATCATCATGCCTGCGTTCATCCTCAACTATCCCATAGACGGATACGACATCAGTGATGAATGGCTCCGGTTCTATCGGC
GGCGAGCCAAAGGTGGGGCCGGCCTGATCATCGTCGGCGCCTGCCATGTGGATCCGGCCGGAAGGCAGGATGAGCATCAG
ATCGGGGCGGACCGGGATGAGTGGTTGCCGTCTCTGGAAAAAGTCGCCAGGGCGATCAAAGAAGGCGGCGCCGTTCCGGC
CCTGCAACTGAACCATGCCGGGCGTTATGCAAAAAAGGCGATCAACGGCGGCTTGGATCCGGTCGCTCCTTCGGCTTTGC
CCTCCCGCTACACGAAGGAGCTGCCGCACGAGTTGACCACTGCAGAGGTAGAAGGGATCATCGAATCTTTTGCCGCAGCT
GCAGGTCGGGCAAAGCGCGCGGGATTCGAGGCCGTTGAATTTCTGGGGGCAACTGGATACCTGATCAGTCAGTTTCTCTC
TCCCCTGACAAATCAGCGCACTGACAGGTTCGGCGGGGATGAAGAGAAGCGCCGGACCTTTATAAGAGAGGTCATCGCAG
CGGTGAAGCGTGAGGTCGGGCATGATTTTCCACTTATCTTCCGGATGTCTTCAACGGACAATATGCCCGGGGGGATGGAT
GATGCGGATCACCGAAACTTTGCGATCGAACTGGAAAAATGGGGGATTCATCTCCTTAACGTAACTGCTGGGTGGCACGA
TGCGCCGGTCCATCAGATAGGTCCCTCCGTTCCCCAAGGCCATTTCATCCCCTATGCGACAAAAATCAAAGAGGTCGTCT
CTATACCAGTTTCGTGCGCCGTCCGTATTACTGAACCCCAAATGGCGCGTCAGGTCATTGCCGAAGGAAAGCTGGACATG
GTAACGCTAGGGAGAGCCCTGATCGTGGATCCAGACTGGCCCAACAAAACTCAGGCGGGAAAGGACGAATCTATCCGCCC
CTGCATTTCATGCTGTAACTGTTTTGACCGCGCCTTTGCCAGGGACAGGATCGAATGCTCGCTGAATGCCGACCTGACCG
ATGATGGTTCGTTGCCGCCTGCCAAACCCAAAAAACGCATACTCGTGGTAGGAGGCGGCCCTGCAGGCATGGAGGCTGCA
CGCGTCCTGGCTACGCGCGGTCACAAGGTGACCCTTCTGGAAAAGAACCGGCAAACAGGCGGAAGGCTTGGCACTGCTGC
AGCCCCCCCACACAAATCCGAGATTGCCGGACTGATCCGCTATCTTACCCATGAACTGGCGGGACTGAAGGTGCCAGTCG
TTGCGGAAACCGACTTTTCACGACTTTCTGAAAATTTCGACGCAGTCATTTTGGCTACCGGCGCCAAGGAGAGAACCATT
CTCATTAATGGAATGGAACAGGTTCCCTGTTACACGAGCAGCGAGATTCTGGATGGCACGGTCTCACCCGACAATCCGGT
GGTCATCATCGGTGCCGGTCTGGTGGGGGGGGAAACCGCCGATTATCTCAGTTCAAAAGGATTTGACGTCTCCCTGGTGG
AGATAAGACCAAAACCGCTTGCTGACATGGGCGCAACCCTGAGGTGGGTTCTTTTGGACCATCTGAACAAGGGGGGGGTG
AATATCCATACCTCATCCTCTGTTCAGGAAATAAAGGATGGAAAAGCGGTCATCCAAACGCTCGAGGGGGTGATTACCGT
CCCGCTTGGATGTCTGGTCATATCTGTCGGCTTTGAGTCAGACGATCTTCTCGTCGGCGATCTTGACCGTGCCGGATTAC
CATATTGCATCATAGGTGACAAGAAATCCCCCCGTAGAATCAAGGATGCGATTCATGAGGGGTTCTTGGCGGCTACTCAC
TGGGTGGACAGTATTTAA

Upstream 100 bases:

>100_bases
ATGGATGGCGGCTGGTCGGCTTTCTAGCAACTCGAGGTCGGAATTTCCGGCTGACCCCGTCAAGAAATACATCGCGGGAA
ACTGCGCAAGGGTTATCACA

Downstream 100 bases:

>100_bases
ATAGTGTAGAAATTGAGAACATTCATGGATTGGCGGGAGAATGGGGGGAAGGTCAAAGTGACTACCGCTTGCGCTTTGTC
CAGTCGTAATTGAACTGGAG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MNTKPVPAEAPDLFSPITISGLTIKNRIIMPAFILNYPIDGYDISDEWLRFYRRRAKGGAGLIIVGACHVDPAGRQDEHQ
IGADRDEWLPSLEKVARAIKEGGAVPALQLNHAGRYAKKAINGGLDPVAPSALPSRYTKELPHELTTAEVEGIIESFAAA
AGRAKRAGFEAVEFLGATGYLISQFLSPLTNQRTDRFGGDEEKRRTFIREVIAAVKREVGHDFPLIFRMSSTDNMPGGMD
DADHRNFAIELEKWGIHLLNVTAGWHDAPVHQIGPSVPQGHFIPYATKIKEVVSIPVSCAVRITEPQMARQVIAEGKLDM
VTLGRALIVDPDWPNKTQAGKDESIRPCISCCNCFDRAFARDRIECSLNADLTDDGSLPPAKPKKRILVVGGGPAGMEAA
RVLATRGHKVTLLEKNRQTGGRLGTAAAPPHKSEIAGLIRYLTHELAGLKVPVVAETDFSRLSENFDAVILATGAKERTI
LINGMEQVPCYTSSEILDGTVSPDNPVVIIGAGLVGGETADYLSSKGFDVSLVEIRPKPLADMGATLRWVLLDHLNKGGV
NIHTSSSVQEIKDGKAVIQTLEGVITVPLGCLVISVGFESDDLLVGDLDRAGLPYCIIGDKKSPRRIKDAIHEGFLAATH
WVDSI

Sequences:

>Translated_645_residues
MNTKPVPAEAPDLFSPITISGLTIKNRIIMPAFILNYPIDGYDISDEWLRFYRRRAKGGAGLIIVGACHVDPAGRQDEHQ
IGADRDEWLPSLEKVARAIKEGGAVPALQLNHAGRYAKKAINGGLDPVAPSALPSRYTKELPHELTTAEVEGIIESFAAA
AGRAKRAGFEAVEFLGATGYLISQFLSPLTNQRTDRFGGDEEKRRTFIREVIAAVKREVGHDFPLIFRMSSTDNMPGGMD
DADHRNFAIELEKWGIHLLNVTAGWHDAPVHQIGPSVPQGHFIPYATKIKEVVSIPVSCAVRITEPQMARQVIAEGKLDM
VTLGRALIVDPDWPNKTQAGKDESIRPCISCCNCFDRAFARDRIECSLNADLTDDGSLPPAKPKKRILVVGGGPAGMEAA
RVLATRGHKVTLLEKNRQTGGRLGTAAAPPHKSEIAGLIRYLTHELAGLKVPVVAETDFSRLSENFDAVILATGAKERTI
LINGMEQVPCYTSSEILDGTVSPDNPVVIIGAGLVGGETADYLSSKGFDVSLVEIRPKPLADMGATLRWVLLDHLNKGGV
NIHTSSSVQEIKDGKAVIQTLEGVITVPLGCLVISVGFESDDLLVGDLDRAGLPYCIIGDKKSPRRIKDAIHEGFLAATH
WVDSI
>Mature_645_residues
MNTKPVPAEAPDLFSPITISGLTIKNRIIMPAFILNYPIDGYDISDEWLRFYRRRAKGGAGLIIVGACHVDPAGRQDEHQ
IGADRDEWLPSLEKVARAIKEGGAVPALQLNHAGRYAKKAINGGLDPVAPSALPSRYTKELPHELTTAEVEGIIESFAAA
AGRAKRAGFEAVEFLGATGYLISQFLSPLTNQRTDRFGGDEEKRRTFIREVIAAVKREVGHDFPLIFRMSSTDNMPGGMD
DADHRNFAIELEKWGIHLLNVTAGWHDAPVHQIGPSVPQGHFIPYATKIKEVVSIPVSCAVRITEPQMARQVIAEGKLDM
VTLGRALIVDPDWPNKTQAGKDESIRPCISCCNCFDRAFARDRIECSLNADLTDDGSLPPAKPKKRILVVGGGPAGMEAA
RVLATRGHKVTLLEKNRQTGGRLGTAAAPPHKSEIAGLIRYLTHELAGLKVPVVAETDFSRLSENFDAVILATGAKERTI
LINGMEQVPCYTSSEILDGTVSPDNPVVIIGAGLVGGETADYLSSKGFDVSLVEIRPKPLADMGATLRWVLLDHLNKGGV
NIHTSSSVQEIKDGKAVIQTLEGVITVPLGCLVISVGFESDDLLVGDLDRAGLPYCIIGDKKSPRRIKDAIHEGFLAATH
WVDSI

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=529, Percent_Identity=34.2155009451796, Blast_Score=291, Evalue=8e-80,
Organism=Escherichia coli, GI1787939, Length=351, Percent_Identity=31.0541310541311, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17540738, Length=347, Percent_Identity=28.2420749279539, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=346, Percent_Identity=26.3005780346821, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=322, Percent_Identity=27.3291925465839, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=322, Percent_Identity=25.4658385093168, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=320, Percent_Identity=25, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=316, Percent_Identity=25.9493670886076, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17566914, Length=318, Percent_Identity=27.6729559748428, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=370, Percent_Identity=25.9459459459459, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=370, Percent_Identity=25.6756756756757, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 69752; Mature: 69752

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKPVPAEAPDLFSPITISGLTIKNRIIMPAFILNYPIDGYDISDEWLRFYRRRAKGGA
CCCCCCCCCCCCCCCCEEEECEEECCCEEHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
GLIIVGACHVDPAGRQDEHQIGADRDEWLPSLEKVARAIKEGGAVPALQLNHAGRYAKKA
CEEEEEEEECCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
INGGLDPVAPSALPSRYTKELPHELTTAEVEGIIESFAAAAGRAKRAGFEAVEFLGATGY
HCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHCCHHH
LISQFLSPLTNQRTDRFGGDEEKRRTFIREVIAAVKREVGHDFPLIFRMSSTDNMPGGMD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
DADHRNFAIELEKWGIHLLNVTAGWHDAPVHQIGPSVPQGHFIPYATKIKEVVSIPVSCA
CCCCCCEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEE
VRITEPQMARQVIAEGKLDMVTLGRALIVDPDWPNKTQAGKDESIRPCISCCNCFDRAFA
EEECCHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RDRIECSLNADLTDDGSLPPAKPKKRILVVGGGPAGMEAARVLATRGHKVTLLEKNRQTG
CCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCC
GRLGTAAAPPHKSEIAGLIRYLTHELAGLKVPVVAETDFSRLSENFDAVILATGAKERTI
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCEE
LINGMEQVPCYTSSEILDGTVSPDNPVVIIGAGLVGGETADYLSSKGFDVSLVEIRPKPL
EEECHHHCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCC
ADMGATLRWVLLDHLNKGGVNIHTSSSVQEIKDGKAVIQTLEGVITVPLGCLVISVGFES
HHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
DDLLVGDLDRAGLPYCIIGDKKSPRRIKDAIHEGFLAATHWVDSI
CCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTKPVPAEAPDLFSPITISGLTIKNRIIMPAFILNYPIDGYDISDEWLRFYRRRAKGGA
CCCCCCCCCCCCCCCCEEEECEEECCCEEHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
GLIIVGACHVDPAGRQDEHQIGADRDEWLPSLEKVARAIKEGGAVPALQLNHAGRYAKKA
CEEEEEEEECCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
INGGLDPVAPSALPSRYTKELPHELTTAEVEGIIESFAAAAGRAKRAGFEAVEFLGATGY
HCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHCCHHH
LISQFLSPLTNQRTDRFGGDEEKRRTFIREVIAAVKREVGHDFPLIFRMSSTDNMPGGMD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
DADHRNFAIELEKWGIHLLNVTAGWHDAPVHQIGPSVPQGHFIPYATKIKEVVSIPVSCA
CCCCCCEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEE
VRITEPQMARQVIAEGKLDMVTLGRALIVDPDWPNKTQAGKDESIRPCISCCNCFDRAFA
EEECCHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RDRIECSLNADLTDDGSLPPAKPKKRILVVGGGPAGMEAARVLATRGHKVTLLEKNRQTG
CCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCC
GRLGTAAAPPHKSEIAGLIRYLTHELAGLKVPVVAETDFSRLSENFDAVILATGAKERTI
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCEE
LINGMEQVPCYTSSEILDGTVSPDNPVVIIGAGLVGGETADYLSSKGFDVSLVEIRPKPL
EEECHHHCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCC
ADMGATLRWVLLDHLNKGGVNIHTSSSVQEIKDGKAVIQTLEGVITVPLGCLVISVGFES
HHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
DDLLVGDLDRAGLPYCIIGDKKSPRRIKDAIHEGFLAATHWVDSI
CCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]