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The map label for this gene is asnB [H]

Identifier: 78223379

GI number: 78223379

Start: 2452716

End: 2454713

Strand: Reverse

Name: asnB [H]

Synonym: Gmet_2172

Alternate gene names: 78223379

Gene position: 2454713-2452716 (Counterclockwise)

Preceding gene: 78223380

Following gene: 78223378

Centisome position: 61.41

GC content: 63.11

Gene sequence:

>1998_bases
ATGTGCGGCATAACCGGTTTCATGGATTTCTCACTGCAATGCGGACCTGACGCCATGCGAGCCAACGCACGGCGCATGGC
GGATGCCCTCATGCACCGCGGCCCCGATGACAGTGGCGCGTGGTGCGACCAGGGGGCGGGTGTCGGCCTGGCGCACCGCC
GGCTCTCCATCCTCGATCTTACCCCTGCCGGCCACCAGCCCATGGAATCGCGTTCCGGGCGGTTCACGATCGTCTTCAAC
GGCGAGATTTACAATCATCAGGCACTGCGGAAGGAGTTGGCGGCGTTCGAGCCCTGGCGGGGGCATTCGGACACGGAGAC
CCTCCTGGCCGGATTCGAGGCATGGGGCATCGAGGCCACGCTCAAGAGGACGGTCGGCATGTTCGCCCTGGCAGTGTGGG
ACAATGCGGAGAGGACGCTGACCCTTGCCCGCGACCGCATGGGTGAGAAGCCCCTGTACTACGGCTGGAACCAGGGCGTT
TTCCTCTTTGCCTCGGAGCTGAAGGCGCTGCGGGCCTATCCGGGTTTCGACGCGGAAATCAACCGCGACGCCATTGCCCT
CCTCATGCGCCACAGTTGCATTCCCACGCCCCACTCGATCTATCGCGGCATCTACAAACTCTGGCCGGGAACCATACTGA
CTCTGCGAAACACGGGCCGCGGAATCGCCCCCTGGGATGCCGCCGATCCCCCCTTCAAGCTGTTTCAGGGAGAGGGGGTT
TCCCTTCGCCCGTACTGGTCATTGCTGGAGGCGGCGGAGCAGGGGCATGCCCGCCCCTTTGAGGGGACGGAAAGCGAGGC
GATAGGGGAACTTGAACGGGTTCTGACGGAAGCCGTGCTGGCGCAGCAGATCTCCGATGTGCCGTTGGGGGCTTTCCTGT
CAGGGGGGGTGGATTCGACCGCCATCGTGGCCCTGATGCAGGCCCACTCGTCCCGGCCGGTGAAGACCTTTACCATCGGT
TTCCACGAGGATGACTGCAACGAGGCGAAATATGCAGGGGAAATCGCCTCCCGCCTGGGGACCGACCATACCGAGCTGTA
CGTGTCGCCCGGTGATTCGCTGGCAGTGATTCCTCGCCTGCCGCAGCTTTATGACGAGCCGTTTTCGGACCCTTCGCACA
TCCCCACGTTTCTGGTGTCCCTGCTGGCACGGGAGCACGTTACGGTTTCCCTGTCCGGAGACGGCGGGGACGAGCTGTTC
GGGGGTTATGTCCGGCACACCTTGCTCCACAGGATCTGGAATAACATCGGCTGGTGCCCGTTGCCGATGCGAAAAGCCCT
GTCCGACAAGATCAGGTTTCTTTCGCCGGGCCAGCTCCAGGCCGTTCTGCGGAATCTTCCCTTCATCGACAGGGTCCACG
CCCCCGTCTACAAGGCCTACCGGCTTGCCGAGCTGATCCGGGCGGATTCCCCCGCATCCATGTACGAGATATGCATCTCC
CACTGGCAGCAGGACGACCCGGTTGTCGTGGGGGGCCAATCCCCCTTCCATGCATCATCCGGCCGGATGCCCCGCCAGCT
CGATTCGTTCAGCCTGCCGGAAAAGATCATGCTCATGGACAGCGTCACCTATCTGCCCGACGATATTCTCGTGAAAATCG
ACCGGGCAGCCATGGGGGTAAGCCTGGAAACGCGGGTGCCGTTCCTCGATCATCGGGTGGTGGAGTTTGCCTGGCGGCTG
CCGCTCGGCCTGAAGATCCGCGGGGGCCAAAGCAAGTGGATTTTGCGCCAGGTGCTGAACAGGCATATCCCGAAAGAGCT
TGTGGAGCGCCCGAAGCATGGCTTCGGCATCCCGCTGGGGACGTGGTTGCGGGGGCCGCTGCGCGAATGGGCCGAGGAGC
TTCTCAATGAGTCCCGGCTGCTGCGCGAAGGGTTTCTGGACCCCAGGCCGGTGCGGCAAAAATGGCTGGAGCACGTTTCC
GGGCGCCGGGATTGGGAATTCCACCTGTGGGACGTGCTGATGTTCCAGTCGTGGCTCGAGCAGGAAAGAAGACCATGA

Upstream 100 bases:

>100_bases
CTCGGGAAAGTATAGCGGGCCGGCTTTCCTCAACTCGACTCCCAAGAGCGTGGCCGGCAACTCTTTCTGAGGGAGCGCGG
CGGCAGAGGGCATTGAACCA

Downstream 100 bases:

>100_bases
AAGTGCTCATGATCTGCAATACCGACGGCGCGCTCTATGTGTTCCGCAAACCGATCATCAACAGGCTGGTCGCCTGCGGC
CACGAGGTTGATTCCATCAG

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MCGITGFMDFSLQCGPDAMRANARRMADALMHRGPDDSGAWCDQGAGVGLAHRRLSILDLTPAGHQPMESRSGRFTIVFN
GEIYNHQALRKELAAFEPWRGHSDTETLLAGFEAWGIEATLKRTVGMFALAVWDNAERTLTLARDRMGEKPLYYGWNQGV
FLFASELKALRAYPGFDAEINRDAIALLMRHSCIPTPHSIYRGIYKLWPGTILTLRNTGRGIAPWDAADPPFKLFQGEGV
SLRPYWSLLEAAEQGHARPFEGTESEAIGELERVLTEAVLAQQISDVPLGAFLSGGVDSTAIVALMQAHSSRPVKTFTIG
FHEDDCNEAKYAGEIASRLGTDHTELYVSPGDSLAVIPRLPQLYDEPFSDPSHIPTFLVSLLAREHVTVSLSGDGGDELF
GGYVRHTLLHRIWNNIGWCPLPMRKALSDKIRFLSPGQLQAVLRNLPFIDRVHAPVYKAYRLAELIRADSPASMYEICIS
HWQQDDPVVVGGQSPFHASSGRMPRQLDSFSLPEKIMLMDSVTYLPDDILVKIDRAAMGVSLETRVPFLDHRVVEFAWRL
PLGLKIRGGQSKWILRQVLNRHIPKELVERPKHGFGIPLGTWLRGPLREWAEELLNESRLLREGFLDPRPVRQKWLEHVS
GRRDWEFHLWDVLMFQSWLEQERRP

Sequences:

>Translated_665_residues
MCGITGFMDFSLQCGPDAMRANARRMADALMHRGPDDSGAWCDQGAGVGLAHRRLSILDLTPAGHQPMESRSGRFTIVFN
GEIYNHQALRKELAAFEPWRGHSDTETLLAGFEAWGIEATLKRTVGMFALAVWDNAERTLTLARDRMGEKPLYYGWNQGV
FLFASELKALRAYPGFDAEINRDAIALLMRHSCIPTPHSIYRGIYKLWPGTILTLRNTGRGIAPWDAADPPFKLFQGEGV
SLRPYWSLLEAAEQGHARPFEGTESEAIGELERVLTEAVLAQQISDVPLGAFLSGGVDSTAIVALMQAHSSRPVKTFTIG
FHEDDCNEAKYAGEIASRLGTDHTELYVSPGDSLAVIPRLPQLYDEPFSDPSHIPTFLVSLLAREHVTVSLSGDGGDELF
GGYVRHTLLHRIWNNIGWCPLPMRKALSDKIRFLSPGQLQAVLRNLPFIDRVHAPVYKAYRLAELIRADSPASMYEICIS
HWQQDDPVVVGGQSPFHASSGRMPRQLDSFSLPEKIMLMDSVTYLPDDILVKIDRAAMGVSLETRVPFLDHRVVEFAWRL
PLGLKIRGGQSKWILRQVLNRHIPKELVERPKHGFGIPLGTWLRGPLREWAEELLNESRLLREGFLDPRPVRQKWLEHVS
GRRDWEFHLWDVLMFQSWLEQERRP
>Mature_665_residues
MCGITGFMDFSLQCGPDAMRANARRMADALMHRGPDDSGAWCDQGAGVGLAHRRLSILDLTPAGHQPMESRSGRFTIVFN
GEIYNHQALRKELAAFEPWRGHSDTETLLAGFEAWGIEATLKRTVGMFALAVWDNAERTLTLARDRMGEKPLYYGWNQGV
FLFASELKALRAYPGFDAEINRDAIALLMRHSCIPTPHSIYRGIYKLWPGTILTLRNTGRGIAPWDAADPPFKLFQGEGV
SLRPYWSLLEAAEQGHARPFEGTESEAIGELERVLTEAVLAQQISDVPLGAFLSGGVDSTAIVALMQAHSSRPVKTFTIG
FHEDDCNEAKYAGEIASRLGTDHTELYVSPGDSLAVIPRLPQLYDEPFSDPSHIPTFLVSLLAREHVTVSLSGDGGDELF
GGYVRHTLLHRIWNNIGWCPLPMRKALSDKIRFLSPGQLQAVLRNLPFIDRVHAPVYKAYRLAELIRADSPASMYEICIS
HWQQDDPVVVGGQSPFHASSGRMPRQLDSFSLPEKIMLMDSVTYLPDDILVKIDRAAMGVSLETRVPFLDHRVVEFAWRL
PLGLKIRGGQSKWILRQVLNRHIPKELVERPKHGFGIPLGTWLRGPLREWAEELLNESRLLREGFLDPRPVRQKWLEHVS
GRRDWEFHLWDVLMFQSWLEQERRP

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=420, Percent_Identity=26.1904761904762, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI168229252, Length=420, Percent_Identity=26.1904761904762, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI168229250, Length=420, Percent_Identity=26.1904761904762, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI296010848, Length=391, Percent_Identity=26.3427109974425, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI296010852, Length=320, Percent_Identity=25.625, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI296010850, Length=320, Percent_Identity=25.625, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1786889, Length=171, Percent_Identity=39.1812865497076, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25147557, Length=176, Percent_Identity=31.25, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71993933, Length=176, Percent_Identity=31.25, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25147560, Length=176, Percent_Identity=31.25, Blast_Score=93, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321563, Length=417, Percent_Identity=26.1390887290168, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325403, Length=419, Percent_Identity=26.491646778043, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45553209, Length=182, Percent_Identity=34.6153846153846, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 74957; Mature: 74957

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGFMDFSLQCGPDAMRANARRMADALMHRGPDDSGAWCDQGAGVGLAHRRLSILDL
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCEEEEEEE
TPAGHQPMESRSGRFTIVFNGEIYNHQALRKELAAFEPWRGHSDTETLLAGFEAWGIEAT
CCCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHH
LKRTVGMFALAVWDNAERTLTLARDRMGEKPLYYGWNQGVFLFASELKALRAYPGFDAEI
HHHHHHHHEEEEECCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCC
NRDAIALLMRHSCIPTPHSIYRGIYKLWPGTILTLRNTGRGIAPWDAADPPFKLFQGEGV
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHCCCCCC
SLRPYWSLLEAAEQGHARPFEGTESEAIGELERVLTEAVLAQQISDVPLGAFLSGGVDST
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHH
AIVALMQAHSSRPVKTFTIGFHEDDCNEAKYAGEIASRLGTDHTELYVSPGDSLAVIPRL
HHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCC
PQLYDEPFSDPSHIPTFLVSLLAREHVTVSLSGDGGDELFGGYVRHTLLHRIWNNIGWCP
HHHHCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
LPMRKALSDKIRFLSPGQLQAVLRNLPFIDRVHAPVYKAYRLAELIRADSPASMYEICIS
CHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HWQQDDPVVVGGQSPFHASSGRMPRQLDSFSLPEKIMLMDSVTYLPDDILVKIDRAAMGV
HCCCCCCEEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCHHEEEEEHHHHCC
SLETRVPFLDHRVVEFAWRLPLGLKIRGGQSKWILRQVLNRHIPKELVERPKHGFGIPLG
CHHCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCHH
TWLRGPLREWAEELLNESRLLREGFLDPRPVRQKWLEHVSGRRDWEFHLWDVLMFQSWLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
QERRP
HHCCC
>Mature Secondary Structure
MCGITGFMDFSLQCGPDAMRANARRMADALMHRGPDDSGAWCDQGAGVGLAHRRLSILDL
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCEEEEEEE
TPAGHQPMESRSGRFTIVFNGEIYNHQALRKELAAFEPWRGHSDTETLLAGFEAWGIEAT
CCCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHH
LKRTVGMFALAVWDNAERTLTLARDRMGEKPLYYGWNQGVFLFASELKALRAYPGFDAEI
HHHHHHHHEEEEECCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCC
NRDAIALLMRHSCIPTPHSIYRGIYKLWPGTILTLRNTGRGIAPWDAADPPFKLFQGEGV
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHCCCCCC
SLRPYWSLLEAAEQGHARPFEGTESEAIGELERVLTEAVLAQQISDVPLGAFLSGGVDST
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHH
AIVALMQAHSSRPVKTFTIGFHEDDCNEAKYAGEIASRLGTDHTELYVSPGDSLAVIPRL
HHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCC
PQLYDEPFSDPSHIPTFLVSLLAREHVTVSLSGDGGDELFGGYVRHTLLHRIWNNIGWCP
HHHHCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
LPMRKALSDKIRFLSPGQLQAVLRNLPFIDRVHAPVYKAYRLAELIRADSPASMYEICIS
CHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HWQQDDPVVVGGQSPFHASSGRMPRQLDSFSLPEKIMLMDSVTYLPDDILVKIDRAAMGV
HCCCCCCEEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCHHEEEEEHHHHCC
SLETRVPFLDHRVVEFAWRLPLGLKIRGGQSKWILRQVLNRHIPKELVERPKHGFGIPLG
CHHCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCHH
TWLRGPLREWAEELLNESRLLREGFLDPRPVRQKWLEHVSGRRDWEFHLWDVLMFQSWLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
QERRP
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]