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The map label for this gene is moaA [H]

Identifier: 78223302

GI number: 78223302

Start: 2358719

End: 2359699

Strand: Reverse

Name: moaA [H]

Synonym: Gmet_2095

Alternate gene names: 78223302

Gene position: 2359699-2358719 (Counterclockwise)

Preceding gene: 78223305

Following gene: 78223301

Centisome position: 59.03

GC content: 56.68

Gene sequence:

>981_bases
ATGTCCCCGTCAGATATCTATGGGAGAAAGATAAATTACCTGCGGTTGTCGGTAACGGATCGCTGCAATCTCCGGTGCAC
GTACTGTATGCCGGCCTCTGGCATCAAGAAGCGCGCACACTCTGACATTCTGAGCTACGAGCAGCTGCATCGGATTGCGG
AGGCGGCAGTTGCCATCGGTGTCGAGAAGATCCGGATAACCGGTGGTGAACCGCTGGTCAGAAAGGGAATGCCCGGCTTT
CTTGCCGGCCTGAAGCGGATTCCCGGCCTGAAGAAACTCGTCCTGACCACAAACGGCGTGTTTCTGGGGAGTATGGCCGG
TGAATTGAGAGACGCCGGTGTTGAAAGTGTCAACATAAGCCTGGATTCGCTGCGTCCCGAAGTGTTTTCCCGGATTACCC
GGCGCGGCGATTTGTCCAAGGTGTTGGAAGGAATCGCCGCTGCCGAGCATGCCGGGTTCGAGCATATCAAGCTGAACATG
GTGGTGATGCGCGGGGTCAACGACGCCGAGGTGCTGGATTTCGCCGCGCTGACCCTCGATAGGCCATTGAAGGTGCGATT
CATCGAGTATATGCCGACGCTGAAGGAACGGAACTGGCAATCCTTCATGGTTCCCGGGGACGAATTGCTGTCCCGGCTTT
CAAAACGGTATCCACTGCGGAAAGTGACCAATGACGCACTTGACGGGCCGGCCGTCTACTACCGGATTGACGGAGCCGCG
GGAACGCTCGGTTTCATAACGCCGATGTCCAGCCATTTTTGCCAGAACTGCAACCGGATCAGGGTCACCTCTTCGGGAAC
GGTAAAAAGCTGTCTGTTTGACGATGGGAAAACGAGCCTCAAACCGTATCTTGAAAATGGCGACGACGCCGGACTGCGGG
AAACACTGCGCCAGGTTGCCGGCACGAAACCCCACAGGCACATGTTCATGTCCCGGGAAGCCGATAACCACTCGTTCACC
ATGTCGCAGATTGGCGGGTAA

Upstream 100 bases:

>100_bases
TCTAAAATATTAAAATGGTACCAAAATACTATAATAACTCGCCCCGGTGGCCAGGTAGACGGCACGTTCTTGAACAGATC
GTTTGATATGTGAGGTGTCT

Downstream 100 bases:

>100_bases
CAGACGTGAAGACCAAAGGAGAATCCGATGGGTAAAGTCATGGCAGTCTGCCTGAGCAAGAACAAAGGCGAACGGAAGAC
CCCGGTGGACAGTGTCCGCT

Product: GTP cyclohydrolase subunit MoaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSPSDIYGRKINYLRLSVTDRCNLRCTYCMPASGIKKRAHSDILSYEQLHRIAEAAVAIGVEKIRITGGEPLVRKGMPGF
LAGLKRIPGLKKLVLTTNGVFLGSMAGELRDAGVESVNISLDSLRPEVFSRITRRGDLSKVLEGIAAAEHAGFEHIKLNM
VVMRGVNDAEVLDFAALTLDRPLKVRFIEYMPTLKERNWQSFMVPGDELLSRLSKRYPLRKVTNDALDGPAVYYRIDGAA
GTLGFITPMSSHFCQNCNRIRVTSSGTVKSCLFDDGKTSLKPYLENGDDAGLRETLRQVAGTKPHRHMFMSREADNHSFT
MSQIGG

Sequences:

>Translated_326_residues
MSPSDIYGRKINYLRLSVTDRCNLRCTYCMPASGIKKRAHSDILSYEQLHRIAEAAVAIGVEKIRITGGEPLVRKGMPGF
LAGLKRIPGLKKLVLTTNGVFLGSMAGELRDAGVESVNISLDSLRPEVFSRITRRGDLSKVLEGIAAAEHAGFEHIKLNM
VVMRGVNDAEVLDFAALTLDRPLKVRFIEYMPTLKERNWQSFMVPGDELLSRLSKRYPLRKVTNDALDGPAVYYRIDGAA
GTLGFITPMSSHFCQNCNRIRVTSSGTVKSCLFDDGKTSLKPYLENGDDAGLRETLRQVAGTKPHRHMFMSREADNHSFT
MSQIGG
>Mature_325_residues
SPSDIYGRKINYLRLSVTDRCNLRCTYCMPASGIKKRAHSDILSYEQLHRIAEAAVAIGVEKIRITGGEPLVRKGMPGFL
AGLKRIPGLKKLVLTTNGVFLGSMAGELRDAGVESVNISLDSLRPEVFSRITRRGDLSKVLEGIAAAEHAGFEHIKLNMV
VMRGVNDAEVLDFAALTLDRPLKVRFIEYMPTLKERNWQSFMVPGDELLSRLSKRYPLRKVTNDALDGPAVYYRIDGAAG
TLGFITPMSSHFCQNCNRIRVTSSGTVKSCLFDDGKTSLKPYLENGDDAGLRETLRQVAGTKPHRHMFMSREADNHSFTM
SQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=327, Percent_Identity=40.9785932721713, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI22027536, Length=327, Percent_Identity=40.9785932721713, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI1786999, Length=332, Percent_Identity=35.5421686746988, Blast_Score=201, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI32566329, Length=328, Percent_Identity=38.719512195122, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI32566331, Length=309, Percent_Identity=39.4822006472492, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17561210, Length=332, Percent_Identity=38.2530120481928, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI28574490, Length=331, Percent_Identity=35.9516616314199, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI28574488, Length=327, Percent_Identity=37.6146788990826, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 36110; Mature: 35979

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSDIYGRKINYLRLSVTDRCNLRCTYCMPASGIKKRAHSDILSYEQLHRIAEAAVAIG
CCCCCCCCCEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VEKIRITGGEPLVRKGMPGFLAGLKRIPGLKKLVLTTNGVFLGSMAGELRDAGVESVNIS
CEEEEEECCCCHHHCCCCHHHHHHHHCCCCEEEEEEECCEEEEHHHHHHHHCCCCEEEEE
LDSLRPEVFSRITRRGDLSKVLEGIAAAEHAGFEHIKLNMVVMRGVNDAEVLDFAALTLD
HHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCC
RPLKVRFIEYMPTLKERNWQSFMVPGDELLSRLSKRYPLRKVTNDALDGPAVYYRIDGAA
CCHHHHHHHHCCHHHCCCCCEEECCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEECCCC
GTLGFITPMSSHFCQNCNRIRVTSSGTVKSCLFDDGKTSLKPYLENGDDAGLRETLRQVA
CCHHHHCCCHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHH
GTKPHRHMFMSREADNHSFTMSQIGG
CCCCHHHHEECCCCCCCCEEHHHCCC
>Mature Secondary Structure 
SPSDIYGRKINYLRLSVTDRCNLRCTYCMPASGIKKRAHSDILSYEQLHRIAEAAVAIG
CCCCCCCCEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VEKIRITGGEPLVRKGMPGFLAGLKRIPGLKKLVLTTNGVFLGSMAGELRDAGVESVNIS
CEEEEEECCCCHHHCCCCHHHHHHHHCCCCEEEEEEECCEEEEHHHHHHHHCCCCEEEEE
LDSLRPEVFSRITRRGDLSKVLEGIAAAEHAGFEHIKLNMVVMRGVNDAEVLDFAALTLD
HHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCC
RPLKVRFIEYMPTLKERNWQSFMVPGDELLSRLSKRYPLRKVTNDALDGPAVYYRIDGAA
CCHHHHHHHHCCHHHCCCCCEEECCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEECCCC
GTLGFITPMSSHFCQNCNRIRVTSSGTVKSCLFDDGKTSLKPYLENGDDAGLRETLRQVA
CCHHHHCCCHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHH
GTKPHRHMFMSREADNHSFTMSQIGG
CCCCHHHHEECCCCCCCCEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA