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The map label for this gene is fadF [H]

Identifier: 78223292

GI number: 78223292

Start: 2345703

End: 2346866

Strand: Reverse

Name: fadF [H]

Synonym: Gmet_2085

Alternate gene names: 78223292

Gene position: 2346866-2345703 (Counterclockwise)

Preceding gene: 78223293

Following gene: 78223291

Centisome position: 58.71

GC content: 61.34

Gene sequence:

>1164_bases
ATGGGTGCCATCGTGGAAACCGTTACTCCATACAAAGAGATCATCGACGTCATCAAAGAGAAGGGGGGCGACTCGCTCAA
GTACTGCTACCAGTGCGGTCTGTGCGATTCGGTCTGCCCCTGGAACCGGGTACGGCAGTTCAGCATGCGCAAGATCGTCC
GTCAGGGGACGTTCGGCCTGACCGAGATCGAACAGGAAGACATCTGGCGCTGCAGCACCTGCGGAACCTGCCCCAGCCGC
TGCCCCAGGGGGGTGAACCAGATTGAGGCCGGGGTCGCCATGCGCCGGATCGGCGCAGAATACGATGTCTATCCCGGCCA
CGTGGGGACTATCCGCAACGTGGTGGCGAGCCTCACCTCCGAGGGTAACTCCCTGGGGGGCGATCGGACCCAGCGGGGCG
ATTGGGCCAAGGACCTGCCGGTCAAGCCCTATGCCGAGGGGATGGAGCTTCTCTACTTCACCGGCTGTTACCTGAGCTAC
GACCCGAGGATGAGAAAGGTGGCCGCTGCTACCGCCGCCATTCTCAACAAGGCCGGGGTTGATTTCGGCATCCTCGGTTC
CAAGGAGAGCTGCTGCGGGGAGAGTATCCGCAAGACCGGTAACGAAGAGCTGTTCAAGCGGCTGGCCAAGGAGAACATCA
AGCAGTTCATCGATAACGGCGTCACGAAGATTCTCGTTTCCTCACCCCACTGCTACCACACCTTCGTGAACGAGTATCCC
GAGTTCAAGGTGAACTTCGAGGTGGTCTTCATCTCCCAGTACATCGGCCAGCTCATCAACGAGGGGCGGCTCCAGATCAC
CGGCGAGTTCGCGAAGAAGGTGACCTATCACGACCCGTGCTACCTGGGGCGCCACAACGGCATCTACGACGAGCCCCGGC
AAGTGCTGCAGCAGGTCCCGGGCCTCGAACTGCTGGAGATGGCGGACAACCGGGAAAGCAGCCTCTGCTGCGGCGGCGGC
GGCGGCCGGATCTGGATGGAAACGCCGAAGGAAGAGCGGTTCGCCGACCTGAGAATCAGGCAGGCGGTAGACGTGGGGGC
TACGGTGCTGGCCACCTCCTGCCCCTACTGCATCACGAACTTCACGGACAGCAGCCTGGATCTGGCGGATCACGAGAAGG
TCGAGGTGAAGGACCTGGCGGAAATTATCCTGGAAGTGATCTAG

Upstream 100 bases:

>100_bases
TGGAAATAGGTCTGGAATCCCTCATCAGCAGATTTGGCGCAGACGTGGTCGCCGATACGGTTGAGCAGCTCACCAAAAAA
CGCTAACTCGACAGAACAGG

Downstream 100 bases:

>100_bases
GAACAACGGAAGCAGAGCATTGAACAGCATTCCAGTCGAAGGGCGAACTCTATGAATACCTCAGACAGAAATATTGGTGA
CGTACTTATTGTCGGCGGAG

Product: hypothetical protein

Products: FADH2; dihydroxy-acetone-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MGAIVETVTPYKEIIDVIKEKGGDSLKYCYQCGLCDSVCPWNRVRQFSMRKIVRQGTFGLTEIEQEDIWRCSTCGTCPSR
CPRGVNQIEAGVAMRRIGAEYDVYPGHVGTIRNVVASLTSEGNSLGGDRTQRGDWAKDLPVKPYAEGMELLYFTGCYLSY
DPRMRKVAAATAAILNKAGVDFGILGSKESCCGESIRKTGNEELFKRLAKENIKQFIDNGVTKILVSSPHCYHTFVNEYP
EFKVNFEVVFISQYIGQLINEGRLQITGEFAKKVTYHDPCYLGRHNGIYDEPRQVLQQVPGLELLEMADNRESSLCCGGG
GGRIWMETPKEERFADLRIRQAVDVGATVLATSCPYCITNFTDSSLDLADHEKVEVKDLAEIILEVI

Sequences:

>Translated_387_residues
MGAIVETVTPYKEIIDVIKEKGGDSLKYCYQCGLCDSVCPWNRVRQFSMRKIVRQGTFGLTEIEQEDIWRCSTCGTCPSR
CPRGVNQIEAGVAMRRIGAEYDVYPGHVGTIRNVVASLTSEGNSLGGDRTQRGDWAKDLPVKPYAEGMELLYFTGCYLSY
DPRMRKVAAATAAILNKAGVDFGILGSKESCCGESIRKTGNEELFKRLAKENIKQFIDNGVTKILVSSPHCYHTFVNEYP
EFKVNFEVVFISQYIGQLINEGRLQITGEFAKKVTYHDPCYLGRHNGIYDEPRQVLQQVPGLELLEMADNRESSLCCGGG
GGRIWMETPKEERFADLRIRQAVDVGATVLATSCPYCITNFTDSSLDLADHEKVEVKDLAEIILEVI
>Mature_386_residues
GAIVETVTPYKEIIDVIKEKGGDSLKYCYQCGLCDSVCPWNRVRQFSMRKIVRQGTFGLTEIEQEDIWRCSTCGTCPSRC
PRGVNQIEAGVAMRRIGAEYDVYPGHVGTIRNVVASLTSEGNSLGGDRTQRGDWAKDLPVKPYAEGMELLYFTGCYLSYD
PRMRKVAAATAAILNKAGVDFGILGSKESCCGESIRKTGNEELFKRLAKENIKQFIDNGVTKILVSSPHCYHTFVNEYPE
FKVNFEVVFISQYIGQLINEGRLQITGEFAKKVTYHDPCYLGRHNGIYDEPRQVLQQVPGLELLEMADNRESSLCCGGGG
GRIWMETPKEERFADLRIRQAVDVGATVLATSCPYCITNFTDSSLDLADHEKVEVKDLAEIILEVI

Specific function: Electron Transfer Protein; May Also Function As The Membrane Anchor For The Glpab Dimer. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788576, Length=388, Percent_Identity=22.1649484536082, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR009051 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 43184; Mature: 43053

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAIVETVTPYKEIIDVIKEKGGDSLKYCYQCGLCDSVCPWNRVRQFSMRKIVRQGTFGL
CCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
TEIEQEDIWRCSTCGTCPSRCPRGVNQIEAGVAMRRIGAEYDVYPGHVGTIRNVVASLTS
CCCCHHHCEECCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EGNSLGGDRTQRGDWAKDLPVKPYAEGMELLYFTGCYLSYDPRMRKVAAATAAILNKAGV
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEECEEEECCHHHHHHHHHHHHHHHHCCC
DFGILGSKESCCGESIRKTGNEELFKRLAKENIKQFIDNGVTKILVSSPHCYHTFVNEYP
CEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCC
EFKVNFEVVFISQYIGQLINEGRLQITGEFAKKVTYHDPCYLGRHNGIYDEPRQVLQQVP
CEEEEEEEEEHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCEECCCCCCCHHHHHHHHHCC
GLELLEMADNRESSLCCGGGGGRIWMETPKEERFADLRIRQAVDVGATVLATSCPYCITN
CHHHHHHHCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHCCCHHHHC
FTDSSLDLADHEKVEVKDLAEIILEVI
CCCCCCCCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
GAIVETVTPYKEIIDVIKEKGGDSLKYCYQCGLCDSVCPWNRVRQFSMRKIVRQGTFGL
CCHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
TEIEQEDIWRCSTCGTCPSRCPRGVNQIEAGVAMRRIGAEYDVYPGHVGTIRNVVASLTS
CCCCHHHCEECCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EGNSLGGDRTQRGDWAKDLPVKPYAEGMELLYFTGCYLSYDPRMRKVAAATAAILNKAGV
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEECEEEECCHHHHHHHHHHHHHHHHCCC
DFGILGSKESCCGESIRKTGNEELFKRLAKENIKQFIDNGVTKILVSSPHCYHTFVNEYP
CEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCC
EFKVNFEVVFISQYIGQLINEGRLQITGEFAKKVTYHDPCYLGRHNGIYDEPRQVLQQVP
CEEEEEEEEEHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCEECCCCCCCHHHHHHHHHCC
GLELLEMADNRESSLCCGGGGGRIWMETPKEERFADLRIRQAVDVGATVLATSCPYCITN
CHHHHHHHCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHCCCHHHHC
FTDSSLDLADHEKVEVKDLAEIILEVI
CCCCCCCCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: FAD; glycerol-3-phosphate [C]

Specific reaction: FAD + glycerol-3-phosphate = FADH2 + dihydroxy-acetone-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]