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The map label for this gene is nuoG [H]

Identifier: 78223286

GI number: 78223286

Start: 2338563

End: 2339219

Strand: Reverse

Name: nuoG [H]

Synonym: Gmet_2079

Alternate gene names: 78223286

Gene position: 2339219-2338563 (Counterclockwise)

Preceding gene: 78223287

Following gene: 78223285

Centisome position: 58.52

GC content: 59.97

Gene sequence:

>657_bases
ATGAGCGAAATCAACTTGCAGATTGACGGGAAAGAGGTTGTCGCCACCGAAGGGATGACCATTCTGGACGCGGCGAAGTC
CGTGGGGATTTCCATCCCCACGCTGTGCCACCACGAGAAGCTTGAGCCTTACGGCGGGTGCCGCATATGTACCGTGGAAG
TGGAGGTCCGCGGGTGGCCGAAGCTGGTCGCCGGCTGCATCTACCCGGTGGAGAAGGGGCTGGTGGTACGAACCAGAAAC
GAAAAGATTGACAAGATCCGCAAGGTGCTGCTGGAGGAGATGCTGGCGCACGCGCCCGACTCCGAGGAGCTGAAGGCGCT
GGCCCAGGAGTACGGGGCGGACAGGGATCGCTTTGAAAAACATCCCTCCTTCTGCATCCACTGCGGCCTCTGTGTCAGGT
ACTGTGCCGAGATAAAAAAGAAGAATGCCGTAGGATTTGTTGACAGGGGGTCAAACCGTGAGATAAGCTTCATTCCGGAA
ATAGCCGCCAAGGAATGCTGGGACTGCAAGGAGTGTTTCCCGCTCTGTCCCACTTCGGCGCTGCAGGCGGCATACGTGCT
GGCCGGGGCGCTGATGACACCTCCCGAGGAGGCGCATTCAGGCGGCTGCGGGTGCTCATGTTCATGCGGCTCCGCGCCGG
AAGAACAGGCCGGATAG

Upstream 100 bases:

>100_bases
TCTGCCCGTCACGCTTCGGCGCGGTGCGGAAGATTTCCGGCGAACCGGTTCCGGCCCCTGTCCCTGAAGACAAACGAGCT
ATTGCCTGAGAGAGAAAACC

Downstream 100 bases:

>100_bases
AATCTTACCTCAAGGAGGAATCACGTGAACCCATTGGCTGTTGAATTGAATGATCTGCTTGCCCAGCACAATCCCCACGT
TCTGGAGATGCTGTCCGATC

Product: 4Fe-4S ferredoxin, iron-sulfur binding protein

Products: NA

Alternate protein names: NADH dehydrogenase I subunit G; NDH-1 subunit G [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSEINLQIDGKEVVATEGMTILDAAKSVGISIPTLCHHEKLEPYGGCRICTVEVEVRGWPKLVAGCIYPVEKGLVVRTRN
EKIDKIRKVLLEEMLAHAPDSEELKALAQEYGADRDRFEKHPSFCIHCGLCVRYCAEIKKKNAVGFVDRGSNREISFIPE
IAAKECWDCKECFPLCPTSALQAAYVLAGALMTPPEEAHSGGCGCSCSCGSAPEEQAG

Sequences:

>Translated_218_residues
MSEINLQIDGKEVVATEGMTILDAAKSVGISIPTLCHHEKLEPYGGCRICTVEVEVRGWPKLVAGCIYPVEKGLVVRTRN
EKIDKIRKVLLEEMLAHAPDSEELKALAQEYGADRDRFEKHPSFCIHCGLCVRYCAEIKKKNAVGFVDRGSNREISFIPE
IAAKECWDCKECFPLCPTSALQAAYVLAGALMTPPEEAHSGGCGCSCSCGSAPEEQAG
>Mature_217_residues
SEINLQIDGKEVVATEGMTILDAAKSVGISIPTLCHHEKLEPYGGCRICTVEVEVRGWPKLVAGCIYPVEKGLVVRTRNE
KIDKIRKVLLEEMLAHAPDSEELKALAQEYGADRDRFEKHPSFCIHCGLCVRYCAEIKKKNAVGFVDRGSNREISFIPEI
AAKECWDCKECFPLCPTSALQAAYVLAGALMTPPEEAHSGGCGCSCSCGSAPEEQAG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG3383

COG function: function code R; Uncharacterized anaerobic dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 2Fe-2S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI33519475, Length=206, Percent_Identity=31.0679611650485, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32566231, Length=210, Percent_Identity=30.4761904761905, Blast_Score=93, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17565758, Length=210, Percent_Identity=30.4761904761905, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI193209088, Length=210, Percent_Identity=30.4761904761905, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640559, Length=209, Percent_Identity=31.1004784688995, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640557, Length=209, Percent_Identity=31.1004784688995, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR006656
- InterPro:   IPR000283
- InterPro:   IPR010228
- InterPro:   IPR019574
- InterPro:   IPR015405 [H]

Pfam domain/function: PF09326 DUF1982; PF00111 Fer2; PF00384 Molybdopterin; PF10588 NADH-G_4Fe-4S_3 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 23733; Mature: 23601

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
7.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEINLQIDGKEVVATEGMTILDAAKSVGISIPTLCHHEKLEPYGGCRICTVEVEVRGWP
CCCEEEEECCCEEEEECCCHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEEEEEEECCCH
KLVAGCIYPVEKGLVVRTRNEKIDKIRKVLLEEMLAHAPDSEELKALAQEYGADRDRFEK
HHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHC
HPSFCIHCGLCVRYCAEIKKKNAVGFVDRGSNREISFIPEIAAKECWDCKECFPLCPTSA
CCCCEEHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCHHHHHHCCCHHHHCCCCCHHH
LQAAYVLAGALMTPPEEAHSGGCGCSCSCGSAPEEQAG
HHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEINLQIDGKEVVATEGMTILDAAKSVGISIPTLCHHEKLEPYGGCRICTVEVEVRGWP
CCEEEEECCCEEEEECCCHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEEEEEEECCCH
KLVAGCIYPVEKGLVVRTRNEKIDKIRKVLLEEMLAHAPDSEELKALAQEYGADRDRFEK
HHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHC
HPSFCIHCGLCVRYCAEIKKKNAVGFVDRGSNREISFIPEIAAKECWDCKECFPLCPTSA
CCCCEEHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCHHHHHHCCCHHHHCCCCCHHH
LQAAYVLAGALMTPPEEAHSGGCGCSCSCGSAPEEQAG
HHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]