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The map label for this gene is miaA

Identifier: 78223259

GI number: 78223259

Start: 2303517

End: 2304446

Strand: Reverse

Name: miaA

Synonym: Gmet_2052

Alternate gene names: 78223259

Gene position: 2304446-2303517 (Counterclockwise)

Preceding gene: 78223260

Following gene: 78223258

Centisome position: 57.65

GC content: 61.08

Gene sequence:

>930_bases
GTGAAGGAAGAAGAAAAGACCAGGCTCGTCATCGTCCAGGGACCCACGGCATCGGGAAAGTCGGCGCTGGCCCTGGAGCT
TGCCGAGCGGATCGGCGGCGAGATCGTCAACGCCGATTCCATGCAGGTCTATCGGGGCATGGACATAGGCACCGCAAAGC
CTTCCCAAGAGGAGCGCCGGCGGGTTCCCCACCACCTCTACGACATTGTCGATCCCAAGGTGAATTTTACCGCCGCCGAC
TTCCGCGAGCACGCATCCCGGGCGATTGCAGATATTGAGCGTCGGGGAAAGCGGGTCATTCTGGTAGGGGGCACCGGTCT
CTACATCAGGATCCTGACCCAGGGGCTCGTGGCTTCTCCCGGCGGGGACGACAATATCCGCCGCGAACTGGAAGATCAGG
CCCACGGCGAAGGCTTGGAGTCCCTGCACCGGCGCCTCGCCGCGGTCGATCCCGTTGCTGCAGCCCGGCTTCACCCCAAT
GACGGCGTCCGGATCGTGCGGGCACTGGAGGTCTTTCTCCTGACGGGGCGTCCCCTGTCGGCCTTTCAGGAAGCGCACCG
CTTTGCCGACGAGCCCTACCGCTGCCTCAAGCTCGGCATTACCGTCGAGCGGGAGCTTCTCTACCGGCGGGTGGAGGAAC
GGGTGGACCGGATGATCGCCGAGGGGCTTGTTGAGGAGGTGAGGGGACTCCTGTCAGCAGGGTATCCCGCAACCCTCAAG
GCCATGGGCTCCATCGGCTACCGGGAAATCTGCGCTCATCTTGCCGGTGAATTCTCCCTCGATGAGGCCATGCGGCTCAT
CAAACAGAACACGCGGCAGTATGCAAAACGCCAGATGACGTGGTTTCGCCGGGATTCGGAAATTATCTGGGTTGAATATC
CGGGGAAGTTTGATAGTATCTTGAGTACTGTCATGGGATTTTATCATTAA

Upstream 100 bases:

>100_bases
CCACCGACTACTCCACCAACTGCCCCCACGGCCGCCCCGTCCTGCAGCGGCTCACCCTTGCCGAGGTGGAGCGGATGTTC
AAACGGTAAGGAGTCATGGC

Downstream 100 bases:

>100_bases
AGGAGCGGCATATGGCAAAAGCACCGTTCAACATCCAGGATCAATACCTCAACCAGTCGCGGAAAGAGCGGATCAAGGTT
GCTGTTCGGCTCATGTCGGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKEEEKTRLVIVQGPTASGKSALALELAERIGGEIVNADSMQVYRGMDIGTAKPSQEERRRVPHHLYDIVDPKVNFTAAD
FREHASRAIADIERRGKRVILVGGTGLYIRILTQGLVASPGGDDNIRRELEDQAHGEGLESLHRRLAAVDPVAAARLHPN
DGVRIVRALEVFLLTGRPLSAFQEAHRFADEPYRCLKLGITVERELLYRRVEERVDRMIAEGLVEEVRGLLSAGYPATLK
AMGSIGYREICAHLAGEFSLDEAMRLIKQNTRQYAKRQMTWFRRDSEIIWVEYPGKFDSILSTVMGFYH

Sequences:

>Translated_309_residues
MKEEEKTRLVIVQGPTASGKSALALELAERIGGEIVNADSMQVYRGMDIGTAKPSQEERRRVPHHLYDIVDPKVNFTAAD
FREHASRAIADIERRGKRVILVGGTGLYIRILTQGLVASPGGDDNIRRELEDQAHGEGLESLHRRLAAVDPVAAARLHPN
DGVRIVRALEVFLLTGRPLSAFQEAHRFADEPYRCLKLGITVERELLYRRVEERVDRMIAEGLVEEVRGLLSAGYPATLK
AMGSIGYREICAHLAGEFSLDEAMRLIKQNTRQYAKRQMTWFRRDSEIIWVEYPGKFDSILSTVMGFYH
>Mature_309_residues
MKEEEKTRLVIVQGPTASGKSALALELAERIGGEIVNADSMQVYRGMDIGTAKPSQEERRRVPHHLYDIVDPKVNFTAAD
FREHASRAIADIERRGKRVILVGGTGLYIRILTQGLVASPGGDDNIRRELEDQAHGEGLESLHRRLAAVDPVAAARLHPN
DGVRIVRALEVFLLTGRPLSAFQEAHRFADEPYRCLKLGITVERELLYRRVEERVDRMIAEGLVEEVRGLLSAGYPATLK
AMGSIGYREICAHLAGEFSLDEAMRLIKQNTRQYAKRQMTWFRRDSEIIWVEYPGKFDSILSTVMGFYH

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=36.9426751592357, Blast_Score=165, Evalue=5e-41,
Organism=Escherichia coli, GI1790613, Length=287, Percent_Identity=44.9477351916376, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=29.9003322259136, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=290, Percent_Identity=31.7241379310345, Blast_Score=159, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24649830, Length=273, Percent_Identity=36.2637362637363, Blast_Score=154, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_GEOMG (Q39TZ3)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385006.1
- ProteinModelPortal:   Q39TZ3
- SMR:   Q39TZ3
- STRING:   Q39TZ3
- GeneID:   3738646
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2052
- NMPDR:   fig|269799.3.peg.1665
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q39TZ3
- ProtClustDB:   PRK00091
- BioCyc:   GMET269799:GMET_2052-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34822; Mature: 34822

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEEKTRLVIVQGPTASGKSALALELAERIGGEIVNADSMQVYRGMDIGTAKPSQEERR
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCHHHHCCCCCCCCCCCHHHHH
RVPHHLYDIVDPKVNFTAADFREHASRAIADIERRGKRVILVGGTGLYIRILTQGLVASP
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCC
GGDDNIRRELEDQAHGEGLESLHRRLAAVDPVAAARLHPNDGVRIVRALEVFLLTGRPLS
CCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHHHCCCCHH
AFQEAHRFADEPYRCLKLGITVERELLYRRVEERVDRMIAEGLVEEVRGLLSAGYPATLK
HHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AMGSIGYREICAHLAGEFSLDEAMRLIKQNTRQYAKRQMTWFRRDSEIIWVEYPGKFDSI
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
LSTVMGFYH
HHHHHHHCC
>Mature Secondary Structure
MKEEEKTRLVIVQGPTASGKSALALELAERIGGEIVNADSMQVYRGMDIGTAKPSQEERR
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCHHHHCCCCCCCCCCCHHHHH
RVPHHLYDIVDPKVNFTAADFREHASRAIADIERRGKRVILVGGTGLYIRILTQGLVASP
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCC
GGDDNIRRELEDQAHGEGLESLHRRLAAVDPVAAARLHPNDGVRIVRALEVFLLTGRPLS
CCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHHHCCCCHH
AFQEAHRFADEPYRCLKLGITVERELLYRRVEERVDRMIAEGLVEEVRGLLSAGYPATLK
HHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AMGSIGYREICAHLAGEFSLDEAMRLIKQNTRQYAKRQMTWFRRDSEIIWVEYPGKFDSI
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
LSTVMGFYH
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA