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The map label for this gene is thiF [C]

Identifier: 78223253

GI number: 78223253

Start: 2298011

End: 2298883

Strand: Reverse

Name: thiF [C]

Synonym: Gmet_2046

Alternate gene names: 78223253

Gene position: 2298883-2298011 (Counterclockwise)

Preceding gene: 78223254

Following gene: 78223252

Centisome position: 57.51

GC content: 51.89

Gene sequence:

>873_bases
ATGGACAGTGATAGCCTCATAAGAAATGAGTTTGCGCGGAACATAGGTCTTCTTACTGTTGAGGAACAAAAACGGCTACT
GGCCTCACGAGTTGCGGTGGCTGGCGCGGGTGGGGTAGGGGGTATTCATGTACTTACTCTGGCTCGGCTTGGCGTTGGCT
GTTTCACTATAGCCGACCATGATTCGTTCGACGTGGTAAACATAAGCAGGCAATTTGGGGCATTCCACAGCACCCTTAAC
CGCAATAAGGCGCAGGTGCTGGCGGAGATGGTGAAGGATATCAACCCGAATGCCGAAGTTCGCGTCATGGAAGAGGGTGT
TACGGAAGACAACATTAATGCATTCCTTGATGGCGTCGATGTCTATGTGGATAGCATAGACTTTTTTGAGATAGATATGC
GTCGCCTCATCTTCAACAGTTGCCGTGCAAAAGGCATCTATGCGCTGACTGCTGCTCCCCTCGGTTTCGGCGCCACGTTG
CAGGTTTTTGACCCTGAAGGGATGAGTTTCGACGACTACTTCGGCATTGATGATCAGACGCCTCCGCTGGAGAAAATTGC
AGCTTTTGCCGCCGGCTTGACTCCGAATCCCTATCATCTTTCCTACATGGATGCCAGCCGGGTGAGCTTCAAGCGGCGTA
CCGGACCGGCGGTATCTCCAGCATGCACCCTGGCCGCTTCACTTGTGGCGACCGAAATCGTGAAGATCCTGACCGGTAAG
GGAGAGCTTCGCCCCATCCCCTGTTATCTTCAATTCGACATGCTTCTTAATAAGGTGAAAATGGGGAAAATAGCATTGGG
GGCGAAGAGTCCATCCCAGAAGAAAAAGCGTCGCCTCATCATGGAAAACCTGCTCATGAAGTCGGAGGATTAG

Upstream 100 bases:

>100_bases
AACTGTGTGATATTTTTTCGATTTGATGGTTGCTGCTACAAGCAGGGCCGGCAAATGTTTTGTTGTTTTATGAATTATTT
CCCACGACGGAGGTTTATAA

Downstream 100 bases:

>100_bases
TCATGAAGTATCGAGAAATACTGGAGGCCGCTGTCCGGGCACCGTCGGGTGAAAACTGCCAGCCATGGCGTTTCGTCATG
GAGGGTGACGTGATACGCAT

Product: hypothetical protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDSDSLIRNEFARNIGLLTVEEQKRLLASRVAVAGAGGVGGIHVLTLARLGVGCFTIADHDSFDVVNISRQFGAFHSTLN
RNKAQVLAEMVKDINPNAEVRVMEEGVTEDNINAFLDGVDVYVDSIDFFEIDMRRLIFNSCRAKGIYALTAAPLGFGATL
QVFDPEGMSFDDYFGIDDQTPPLEKIAAFAAGLTPNPYHLSYMDASRVSFKRRTGPAVSPACTLAASLVATEIVKILTGK
GELRPIPCYLQFDMLLNKVKMGKIALGAKSPSQKKKRRLIMENLLMKSED

Sequences:

>Translated_290_residues
MDSDSLIRNEFARNIGLLTVEEQKRLLASRVAVAGAGGVGGIHVLTLARLGVGCFTIADHDSFDVVNISRQFGAFHSTLN
RNKAQVLAEMVKDINPNAEVRVMEEGVTEDNINAFLDGVDVYVDSIDFFEIDMRRLIFNSCRAKGIYALTAAPLGFGATL
QVFDPEGMSFDDYFGIDDQTPPLEKIAAFAAGLTPNPYHLSYMDASRVSFKRRTGPAVSPACTLAASLVATEIVKILTGK
GELRPIPCYLQFDMLLNKVKMGKIALGAKSPSQKKKRRLIMENLLMKSED
>Mature_290_residues
MDSDSLIRNEFARNIGLLTVEEQKRLLASRVAVAGAGGVGGIHVLTLARLGVGCFTIADHDSFDVVNISRQFGAFHSTLN
RNKAQVLAEMVKDINPNAEVRVMEEGVTEDNINAFLDGVDVYVDSIDFFEIDMRRLIFNSCRAKGIYALTAAPLGFGATL
QVFDPEGMSFDDYFGIDDQTPPLEKIAAFAAGLTPNPYHLSYMDASRVSFKRRTGPAVSPACTLAASLVATEIVKILTGK
GELRPIPCYLQFDMLLNKVKMGKIALGAKSPSQKKKRRLIMENLLMKSED

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Escherichia coli, GI87082356, Length=240, Percent_Identity=27.5, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789177, Length=154, Percent_Identity=32.4675324675325, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 31726; Mature: 31726

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSDSLIRNEFARNIGLLTVEEQKRLLASRVAVAGAGGVGGIHVLTLARLGVGCFTIADH
CCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECC
DSFDVVNISRQFGAFHSTLNRNKAQVLAEMVKDINPNAEVRVMEEGVTEDNINAFLDGVD
CCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHH
VYVDSIDFFEIDMRRLIFNSCRAKGIYALTAAPLGFGATLQVFDPEGMSFDDYFGIDDQT
HEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCC
PPLEKIAAFAAGLTPNPYHLSYMDASRVSFKRRTGPAVSPACTLAASLVATEIVKILTGK
CCHHHHHHHHCCCCCCCEEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GELRPIPCYLQFDMLLNKVKMGKIALGAKSPSQKKKRRLIMENLLMKSED
CCCEECCCEEEHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDSDSLIRNEFARNIGLLTVEEQKRLLASRVAVAGAGGVGGIHVLTLARLGVGCFTIADH
CCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECC
DSFDVVNISRQFGAFHSTLNRNKAQVLAEMVKDINPNAEVRVMEEGVTEDNINAFLDGVD
CCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHH
VYVDSIDFFEIDMRRLIFNSCRAKGIYALTAAPLGFGATLQVFDPEGMSFDDYFGIDDQT
HEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCC
PPLEKIAAFAAGLTPNPYHLSYMDASRVSFKRRTGPAVSPACTLAASLVATEIVKILTGK
CCHHHHHHHHCCCCCCCEEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GELRPIPCYLQFDMLLNKVKMGKIALGAKSPSQKKKRRLIMENLLMKSED
CCCEECCCEEEHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]