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The map label for this gene is zraS [H]

Identifier: 78223242

GI number: 78223242

Start: 2278713

End: 2279747

Strand: Reverse

Name: zraS [H]

Synonym: Gmet_2035

Alternate gene names: 78223242

Gene position: 2279747-2278713 (Counterclockwise)

Preceding gene: 78223245

Following gene: 78223241

Centisome position: 57.03

GC content: 50.92

Gene sequence:

>1035_bases
ATGGTTTTTTATTCATTCGACGTAGCACCGAATCTAACTCTTTCCTCCTGTGACGAGGCTGCGGAACAAATAATCGGAAC
TCCTGCAGCCCTTCTCGCCGGAAAGCCTTATTATGAAGTGTTCCCGCGAGTATTGTATCAGGACCGCGATGCGGTGGCGG
AGGTCGTCCAAAGCGGCATTCCTCTATTGCTGCGAGATTATTCGGTTATTTGTATTTTGGGAAGGACTTTGGCTGATATA
CGTTTAGAGCCCGTCCTGAATCAGGAGAGCGCTGTGGGGGCGGTTCGGGTTTCCGTGTGCGCTTCCGAAACCTGTTCAAG
AGCCTTGGAGTTGAAAGGGGCCAGTACGCTTATCGATATTGGCAAGGTGTCCGCCACTCTTGCCCATGGCGTTCGAAATC
CGCTCAACGCGATAAAGGGGGCAGTAGTATATCTCAAGCACCGCTATGGCTCCGATGAAACATTTGTGGAATTTGCGGAC
ATCATCGACGAGGAGATTGCAAAGCTCGATGGATTCATAACCAAGTTCCTCAGTACGTCGCTCCTGGAGATGGAAAAATC
GCGCAGCGACCTCCATGTGCTTCTTGAAAAAGTATTGAAGATTACATCGCTTCAGGCACAGGAACGCGATATCCGGTTTG
TCTGCGATTACGGGGATGTTCCGTTCCTCGAGTTTGATGCTTTCCATCTGGAGAGTGCAATACTCAACATTGTCAACAAT
GCCATTGAGGCTATGGGGAGCGGCGGCACCATTACGTTCAGAACCCGTTTGGACACTATGGCGGACAGGAGACAGGTGAC
ACTCGATATCTCAGATTCCGGGCCGGGGATACCTGAGGGGGCTTTCCACAATCTGCTTTTACCACCACGTGGCAACACTC
GGGACAAGGGGCGGGGGTTCGGTCTTTTTATCTCTCGGGAGATCATTCAGTATCACGGTGGAAGTCTTGAAATAATAAAC
TCCAAGGGAAACGGTGCGACCGTGCGTCTCAGCCTTCCTGTAGAGAGTTCTCATGGAGAAACTCATGCAGGGTAA

Upstream 100 bases:

>100_bases
TAACCGCTGCTCCTAGTAAAGTCAAATAGTGTCTTTTCAAGGCGAGGCAGAGAGTGAATACTGAACAAATTCATTCTCTG
TTAATCACCTAGGTAATCCA

Downstream 100 bases:

>100_bases
AGCGCACGGGAAGGTGCTCATTGTCGATGATGAGCCGAATGCCCTGAAGGTGCTCGCTGCAATCCTGCGGGAGGAAGGTT
ATTCTGTATTCAAGGCCGGC

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MVFYSFDVAPNLTLSSCDEAAEQIIGTPAALLAGKPYYEVFPRVLYQDRDAVAEVVQSGIPLLLRDYSVICILGRTLADI
RLEPVLNQESAVGAVRVSVCASETCSRALELKGASTLIDIGKVSATLAHGVRNPLNAIKGAVVYLKHRYGSDETFVEFAD
IIDEEIAKLDGFITKFLSTSLLEMEKSRSDLHVLLEKVLKITSLQAQERDIRFVCDYGDVPFLEFDAFHLESAILNIVNN
AIEAMGSGGTITFRTRLDTMADRRQVTLDISDSGPGIPEGAFHNLLLPPRGNTRDKGRGFGLFISREIIQYHGGSLEIIN
SKGNGATVRLSLPVESSHGETHAG

Sequences:

>Translated_344_residues
MVFYSFDVAPNLTLSSCDEAAEQIIGTPAALLAGKPYYEVFPRVLYQDRDAVAEVVQSGIPLLLRDYSVICILGRTLADI
RLEPVLNQESAVGAVRVSVCASETCSRALELKGASTLIDIGKVSATLAHGVRNPLNAIKGAVVYLKHRYGSDETFVEFAD
IIDEEIAKLDGFITKFLSTSLLEMEKSRSDLHVLLEKVLKITSLQAQERDIRFVCDYGDVPFLEFDAFHLESAILNIVNN
AIEAMGSGGTITFRTRLDTMADRRQVTLDISDSGPGIPEGAFHNLLLPPRGNTRDKGRGFGLFISREIIQYHGGSLEIIN
SKGNGATVRLSLPVESSHGETHAG
>Mature_344_residues
MVFYSFDVAPNLTLSSCDEAAEQIIGTPAALLAGKPYYEVFPRVLYQDRDAVAEVVQSGIPLLLRDYSVICILGRTLADI
RLEPVLNQESAVGAVRVSVCASETCSRALELKGASTLIDIGKVSATLAHGVRNPLNAIKGAVVYLKHRYGSDETFVEFAD
IIDEEIAKLDGFITKFLSTSLLEMEKSRSDLHVLLEKVLKITSLQAQERDIRFVCDYGDVPFLEFDAFHLESAILNIVNN
AIEAMGSGGTITFRTRLDTMADRRQVTLDISDSGPGIPEGAFHNLLLPPRGNTRDKGRGFGLFISREIIQYHGGSLEIIN
SKGNGATVRLSLPVESSHGETHAG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=29.0748898678414, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790300, Length=206, Percent_Identity=31.5533980582524, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=28.3261802575107, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790861, Length=214, Percent_Identity=28.0373831775701, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 37471; Mature: 37471

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFYSFDVAPNLTLSSCDEAAEQIIGTPAALLAGKPYYEVFPRVLYQDRDAVAEVVQSGI
CEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCC
PLLLRDYSVICILGRTLADIRLEPVLNQESAVGAVRVSVCASETCSRALELKGASTLIDI
CEEECCCEEEEEECHHHHHEEECCCCCCCCCHHHEEHHHHHHHHHHHHHHCCCCHHEEEH
GKVSATLAHGVRNPLNAIKGAVVYLKHRYGSDETFVEFADIIDEEIAKLDGFITKFLSTS
HHHHHHHHHHHCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEMEKSRSDLHVLLEKVLKITSLQAQERDIRFVCDYGDVPFLEFDAFHLESAILNIVNN
HHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHH
AIEAMGSGGTITFRTRLDTMADRRQVTLDISDSGPGIPEGAFHNLLLPPRGNTRDKGRGF
HHHHCCCCCEEEEEEEHHHHHCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCE
GLFISREIIQYHGGSLEIINSKGNGATVRLSLPVESSHGETHAG
EEHHHHHHHHHCCCEEEEEECCCCCEEEEEEECEECCCCCCCCC
>Mature Secondary Structure
MVFYSFDVAPNLTLSSCDEAAEQIIGTPAALLAGKPYYEVFPRVLYQDRDAVAEVVQSGI
CEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCC
PLLLRDYSVICILGRTLADIRLEPVLNQESAVGAVRVSVCASETCSRALELKGASTLIDI
CEEECCCEEEEEECHHHHHEEECCCCCCCCCHHHEEHHHHHHHHHHHHHHCCCCHHEEEH
GKVSATLAHGVRNPLNAIKGAVVYLKHRYGSDETFVEFADIIDEEIAKLDGFITKFLSTS
HHHHHHHHHHHCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEMEKSRSDLHVLLEKVLKITSLQAQERDIRFVCDYGDVPFLEFDAFHLESAILNIVNN
HHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHH
AIEAMGSGGTITFRTRLDTMADRRQVTLDISDSGPGIPEGAFHNLLLPPRGNTRDKGRGF
HHHHCCCCCEEEEEEEHHHHHCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCE
GLFISREIIQYHGGSLEIINSKGNGATVRLSLPVESSHGETHAG
EEHHHHHHHHHCCCEEEEEECCCCCEEEEEEECEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]