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The map label for this gene is tapB [H]

Identifier: 78223071

GI number: 78223071

Start: 2076422

End: 2078143

Strand: Reverse

Name: tapB [H]

Synonym: Gmet_1864

Alternate gene names: 78223071

Gene position: 2078143-2076422 (Counterclockwise)

Preceding gene: 78223072

Following gene: 78223070

Centisome position: 51.99

GC content: 55.05

Gene sequence:

>1722_bases
ATGAAGCATGTCTTTAAAAGGAAGAATATCGGCGAAATTCTTATCGAACGCGGGTCATTGAGAACCGATGACCTTCCCTT
CGTCGTCGAAAGACTGAAGGCGACCGCAAAGCGTTTCGGGGAGATATGTGTCGAGGAAGGCTTCATTACCGAAGAGTCCC
TGGCGGAGTCCCTGGCGGAGCAATTCGGCTTGGAGTACGTCGATGCCCGGGGACTGCGCCCCAACGAGGCAATTCTCAAC
GCGCTTCCTCCCGATGCCATTTACCGGTTCCGATTTGTTCCCTTGGAGGAGCAGGACAACAGCCTTGTCATCCTCATCTC
TGATCCCACCGATGTCATAAAACTTGACGAGCTTGAACTGCTCCTCGATCGGCCTCTTATCATTAAATTGGCCACTGAAT
CGGCGATTTCCGCCATCCTGAAAAAAGGAGAGGCCACGAGCCGGGTCCTCAAGGAAGTTTCCGAGGACTTCATGCTCCAA
CTCGTGCGGGAGAACGAGACGGGTGAGGAGATCCTCTCCATGGAGAAGATCTCCGCCGATACGAGCCCGATCATTAAGCT
GGTCAACTCTACCGTCATGGATGCCCTGACGCGAAGGGCAAGCGATATCCATATCGAAACGGCGTTGGAAGGGGTTGTCA
TCAAATACCGGATCGATGGGGTTCTCTACCGAGCAACGGAGACACTCGACGTCCATTTCCAGGCACCGATCATCTCACGC
CTCAAGGTCATGAGCGAGCTCGACATTTCAGAGCGGCGCATTCCCCAGGATGGACGTTTCAAGGTCCGGATTGCCGACAA
GGCCATCGATTTCCGCGTTTCTATCATGCCGAGCGCCTTCGGAGAAGACGCTGTTATCCGTATCCTCGACAAGGAGAGCA
TCGCCTCCGATCTCAAGGGGCTGACCCTTGAAACCCTTGGCATGAACCCGCGGGAGATGAAAAGACTGCGGAAAAAGGTC
CGCGAGCCCTACGGGATGGTGCTAGTAACCGGACCAACCGGGTCAGGCAAGACCACGACCCTGTATGCTGCTCTTACCGA
GATTCATACCGGTGAGGAAAAGATCATCACCATCGAGGATCCTGTTGAGTATGCCTTGCGGGGGATTGTCCAGATTCCGG
TCAACGAGAAGAAGGGGCTGACCTTTGCCCGGGGATTGCGCTCAATCCTTCGGCACGACCCTGACAAGATCATGGTGGGG
GAGATCCGCGATCCTGAAACTGCTCAGATCGCCGTGCAATCCGCACTGACGGGACACTTGGTATTCACCACGGTACACGC
CAATAACGTGTTTGACGTTCTCGGTCGTTTTATTCACATGGGTATCGACCCCTACAACTTCGTTTCCTGCCTGAACTGCG
TCATGGCCCAGCGATTGGTGCGCAAGCTTTGCTCCCACTGCAAGTTGCCGACTGTGCATCCTGATTCGGTACTCATTGAA
TCAGGACTTGACCCGGAGCAGTGTCGCAGCGTAACCTTTTTCGATGCGCGCGGGTGCGACGAGTGTAACGGCACTGGTTA
TCGCGGTCGCTCCGCCATAATTGAGCTGCTTGATCTCAACGACCACATCCGTGAACTGATCATGTCTAAGACGCCGGCTG
CCCAACTCAAGGTGGCCGCCCGGGAAGCGGGAACGGTTTTCCTTCGCGAGTCGGCTGTGGAAAAGGTCTTGGCCGGAGAG
ACGTCTCTGCGGGAAATCAATCGCGTCACCTTTGTTGAATAG

Upstream 100 bases:

>100_bases
ATTACCATGTATCTTCCGGTATTCAAGATCGCTGGGACAGTTGGATAATCAAGGGTCGGAGACTAATTGCGGGCACAGGG
CCTGATCGGATCAAACCATT

Downstream 100 bases:

>100_bases
CGCTTGGTCCACTTTATCGATGAAAGGCTTTCATGTTTTTTTCCCGTAATGCAGTGGGGATGGAGATAACCCAGCATGGG
GTCAGGATGGCAGCGGTTGG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKHVFKRKNIGEILIERGSLRTDDLPFVVERLKATAKRFGEICVEEGFITEESLAESLAEQFGLEYVDARGLRPNEAILN
ALPPDAIYRFRFVPLEEQDNSLVILISDPTDVIKLDELELLLDRPLIIKLATESAISAILKKGEATSRVLKEVSEDFMLQ
LVRENETGEEILSMEKISADTSPIIKLVNSTVMDALTRRASDIHIETALEGVVIKYRIDGVLYRATETLDVHFQAPIISR
LKVMSELDISERRIPQDGRFKVRIADKAIDFRVSIMPSAFGEDAVIRILDKESIASDLKGLTLETLGMNPREMKRLRKKV
REPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIEDPVEYALRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMVG
EIRDPETAQIAVQSALTGHLVFTTVHANNVFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKLCSHCKLPTVHPDSVLIE
SGLDPEQCRSVTFFDARGCDECNGTGYRGRSAIIELLDLNDHIRELIMSKTPAAQLKVAAREAGTVFLRESAVEKVLAGE
TSLREINRVTFVE

Sequences:

>Translated_573_residues
MKHVFKRKNIGEILIERGSLRTDDLPFVVERLKATAKRFGEICVEEGFITEESLAESLAEQFGLEYVDARGLRPNEAILN
ALPPDAIYRFRFVPLEEQDNSLVILISDPTDVIKLDELELLLDRPLIIKLATESAISAILKKGEATSRVLKEVSEDFMLQ
LVRENETGEEILSMEKISADTSPIIKLVNSTVMDALTRRASDIHIETALEGVVIKYRIDGVLYRATETLDVHFQAPIISR
LKVMSELDISERRIPQDGRFKVRIADKAIDFRVSIMPSAFGEDAVIRILDKESIASDLKGLTLETLGMNPREMKRLRKKV
REPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIEDPVEYALRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMVG
EIRDPETAQIAVQSALTGHLVFTTVHANNVFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKLCSHCKLPTVHPDSVLIE
SGLDPEQCRSVTFFDARGCDECNGTGYRGRSAIIELLDLNDHIRELIMSKTPAAQLKVAAREAGTVFLRESAVEKVLAGE
TSLREINRVTFVE
>Mature_573_residues
MKHVFKRKNIGEILIERGSLRTDDLPFVVERLKATAKRFGEICVEEGFITEESLAESLAEQFGLEYVDARGLRPNEAILN
ALPPDAIYRFRFVPLEEQDNSLVILISDPTDVIKLDELELLLDRPLIIKLATESAISAILKKGEATSRVLKEVSEDFMLQ
LVRENETGEEILSMEKISADTSPIIKLVNSTVMDALTRRASDIHIETALEGVVIKYRIDGVLYRATETLDVHFQAPIISR
LKVMSELDISERRIPQDGRFKVRIADKAIDFRVSIMPSAFGEDAVIRILDKESIASDLKGLTLETLGMNPREMKRLRKKV
REPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIEDPVEYALRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMVG
EIRDPETAQIAVQSALTGHLVFTTVHANNVFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKLCSHCKLPTVHPDSVLIE
SGLDPEQCRSVTFFDARGCDECNGTGYRGRSAIIELLDLNDHIRELIMSKTPAAQLKVAAREAGTVFLRESAVEKVLAGE
TSLREINRVTFVE

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=442, Percent_Identity=41.1764705882353, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1786296, Length=390, Percent_Identity=42.3076923076923, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI87082188, Length=272, Percent_Identity=32.7205882352941, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 64047; Mature: 64047

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHVFKRKNIGEILIERGSLRTDDLPFVVERLKATAKRFGEICVEEGFITEESLAESLAE
CCCCHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
QFGLEYVDARGLRPNEAILNALPPDAIYRFRFVPLEEQDNSLVILISDPTDVIKLDELEL
HHCCEEECCCCCCCCHHHHHCCCCCHHHEEEEEECCCCCCEEEEEECCCCCEEEHHHHHH
LLDRPLIIKLATESAISAILKKGEATSRVLKEVSEDFMLQLVRENETGEEILSMEKISAD
HHCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
TSPIIKLVNSTVMDALTRRASDIHIETALEGVVIKYRIDGVLYRATETLDVHFQAPIISR
HHHHHHHHHHHHHHHHHHHHCCEEEEEHHCCEEEEEEECEEEEEEEEEEEEEECCHHHHH
LKVMSELDISERRIPQDGRFKVRIADKAIDFRVSIMPSAFGEDAVIRILDKESIASDLKG
HHHHHHCCCHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEEECHHHHHHHHCC
LTLETLGMNPREMKRLRKKVREPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIED
CEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHEEEHHHHCCCCCEEEEECC
PVEYALRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDPETAQIAVQSALTGHL
HHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCEE
VFTTVHANNVFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKLCSHCKLPTVHPDSVLIE
EEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
SGLDPEQCRSVTFFDARGCDECNGTGYRGRSAIIELLDLNDHIRELIMSKTPAAQLKVAA
CCCCHHHHCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHEEHH
REAGTVFLRESAVEKVLAGETSLREINRVTFVE
HHCCEEEEHHHHHHHHHCCCHHHHHHCHHCCCC
>Mature Secondary Structure
MKHVFKRKNIGEILIERGSLRTDDLPFVVERLKATAKRFGEICVEEGFITEESLAESLAE
CCCCHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
QFGLEYVDARGLRPNEAILNALPPDAIYRFRFVPLEEQDNSLVILISDPTDVIKLDELEL
HHCCEEECCCCCCCCHHHHHCCCCCHHHEEEEEECCCCCCEEEEEECCCCCEEEHHHHHH
LLDRPLIIKLATESAISAILKKGEATSRVLKEVSEDFMLQLVRENETGEEILSMEKISAD
HHCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
TSPIIKLVNSTVMDALTRRASDIHIETALEGVVIKYRIDGVLYRATETLDVHFQAPIISR
HHHHHHHHHHHHHHHHHHHHCCEEEEEHHCCEEEEEEECEEEEEEEEEEEEEECCHHHHH
LKVMSELDISERRIPQDGRFKVRIADKAIDFRVSIMPSAFGEDAVIRILDKESIASDLKG
HHHHHHCCCHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEEECHHHHHHHHCC
LTLETLGMNPREMKRLRKKVREPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIED
CEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHEEEHHHHCCCCCEEEEECC
PVEYALRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDPETAQIAVQSALTGHL
HHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCEE
VFTTVHANNVFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKLCSHCKLPTVHPDSVLIE
EEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
SGLDPEQCRSVTFFDARGCDECNGTGYRGRSAIIELLDLNDHIRELIMSKTPAAQLKVAA
CCCCHHHHCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHEEHH
REAGTVFLRESAVEKVLAGETSLREINRVTFVE
HHCCEEEEHHHHHHHHHCCCHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]