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The map label for this gene is purN [H]

Identifier: 78223052

GI number: 78223052

Start: 2057786

End: 2058406

Strand: Direct

Name: purN [H]

Synonym: Gmet_1845

Alternate gene names: 78223052

Gene position: 2057786-2058406 (Clockwise)

Preceding gene: 78223051

Following gene: 78223053

Centisome position: 51.48

GC content: 57.81

Gene sequence:

>621_bases
ATGACTGATCTTCTTACTATCGGCGTTCTTGTCTCAGGAAACGGCTCTAACCTCCAGGCCATCATTGACCGGATCGAGGA
CGGTAGCCTCCCAGCCCGAATTGCCTGCGTCATCAGCAACAAGGCCGATGCCTATGCTTTGGACCGGGCGAAGTGCCACG
GTATCACCGTCCACGTCCTCGACCACAGGATCCATGCGGGCCGTGAATCGTACGACGCAGCCCTGGTTGAACTGCTTCGC
TCCCACGGGGTGCGCCTCGTGGTGCTGGCCGGCTTCATGCGGATCGTTACCCCGGTCCTTATTGGAGCATTTCCCCATGC
AATCATGAACATCCATCCGGCGTTACTTCCGGCATTCCCTGGCCTTCACGCCCAACGTCAGGCTTTGCAGTACGGTGTCA
AAGTCTCTGGGTGCACGGTGCATTTCGTTGATGAAGGAACCGACACTGGACCGATTATTATTCAAGCCGTCGTACCGGTT
CTCGACGATGACACCGAGGATACGCTATCGGCCCGCATCCAGAAGGAAGAGCACCACATCTACCCTGAAGCGGTCAATCT
CTTTGCCCAGGGCCGCCTCACCGTCGACGACCGGAAGGTTATCATCGCGCCGGCATCCTGA

Upstream 100 bases:

>100_bases
CGCCTCTCGGGACTCCAAGAGAAGGCTTTCGTCATCGGCGAAGTGGCCAAGTGTGAGGCCGGCACCGAGTCGGTGGAAAT
GATCTAAACGGTTAATTCCC

Downstream 100 bases:

>100_bases
ACACGGTGCACGCATCAGTCGCACGTCCATTCACAGTATCCGGCTTAATGCAGGAATTCATGATACAGCCAAAGGACTCT
CGTCATGAAAAGGACCGTAC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTDLLTIGVLVSGNGSNLQAIIDRIEDGSLPARIACVISNKADAYALDRAKCHGITVHVLDHRIHAGRESYDAALVELLR
SHGVRLVVLAGFMRIVTPVLIGAFPHAIMNIHPALLPAFPGLHAQRQALQYGVKVSGCTVHFVDEGTDTGPIIIQAVVPV
LDDDTEDTLSARIQKEEHHIYPEAVNLFAQGRLTVDDRKVIIAPAS

Sequences:

>Translated_206_residues
MTDLLTIGVLVSGNGSNLQAIIDRIEDGSLPARIACVISNKADAYALDRAKCHGITVHVLDHRIHAGRESYDAALVELLR
SHGVRLVVLAGFMRIVTPVLIGAFPHAIMNIHPALLPAFPGLHAQRQALQYGVKVSGCTVHFVDEGTDTGPIIIQAVVPV
LDDDTEDTLSARIQKEEHHIYPEAVNLFAQGRLTVDDRKVIIAPAS
>Mature_205_residues
TDLLTIGVLVSGNGSNLQAIIDRIEDGSLPARIACVISNKADAYALDRAKCHGITVHVLDHRIHAGRESYDAALVELLRS
HGVRLVVLAGFMRIVTPVLIGAFPHAIMNIHPALLPAFPGLHAQRQALQYGVKVSGCTVHFVDEGTDTGPIIIQAVVPVL
DDDTEDTLSARIQKEEHHIYPEAVNLFAQGRLTVDDRKVIIAPAS

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=195, Percent_Identity=44.1025641025641, Blast_Score=168, Evalue=3e-42,
Organism=Homo sapiens, GI209869995, Length=195, Percent_Identity=44.1025641025641, Blast_Score=168, Evalue=3e-42,
Organism=Homo sapiens, GI209869993, Length=195, Percent_Identity=44.1025641025641, Blast_Score=168, Evalue=3e-42,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=47.1204188481675, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787483, Length=197, Percent_Identity=30.9644670050761, Blast_Score=88, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=43.5754189944134, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320616, Length=195, Percent_Identity=30.2564102564103, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=49.7326203208556, Blast_Score=183, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22225; Mature: 22094

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLLTIGVLVSGNGSNLQAIIDRIEDGSLPARIACVISNKADAYALDRAKCHGITVHVL
CCCEEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEEECCHHHCCEEEEEE
DHRIHAGRESYDAALVELLRSHGVRLVVLAGFMRIVTPVLIGAFPHAIMNIHPALLPAFP
HHHHHCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
GLHAQRQALQYGVKVSGCTVHFVDEGTDTGPIIIQAVVPVLDDDTEDTLSARIQKEEHHI
CCHHHHHHHHCCCEECCEEEEEEECCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHCCC
YPEAVNLFAQGRLTVDDRKVIIAPAS
CHHHHHHHHCCEEEECCCEEEEEECC
>Mature Secondary Structure 
TDLLTIGVLVSGNGSNLQAIIDRIEDGSLPARIACVISNKADAYALDRAKCHGITVHVL
CCEEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEEECCHHHCCEEEEEE
DHRIHAGRESYDAALVELLRSHGVRLVVLAGFMRIVTPVLIGAFPHAIMNIHPALLPAFP
HHHHHCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
GLHAQRQALQYGVKVSGCTVHFVDEGTDTGPIIIQAVVPVLDDDTEDTLSARIQKEEHHI
CCHHHHHHHHCCCEECCEEEEEEECCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHCCC
YPEAVNLFAQGRLTVDDRKVIIAPAS
CHHHHHHHHCCEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]