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The map label for this gene is paaK [H]

Identifier: 78223032

GI number: 78223032

Start: 2038526

End: 2039833

Strand: Reverse

Name: paaK [H]

Synonym: Gmet_1825

Alternate gene names: 78223032

Gene position: 2039833-2038526 (Counterclockwise)

Preceding gene: 78223033

Following gene: 78223031

Centisome position: 51.03

GC content: 59.4

Gene sequence:

>1308_bases
ATGAGCTATTACAATGAAGAGTTCGAGACGCTTCCCCGCCAAGCCCTGGAGGCCCTGCAGCTCAAACGGCTTCAGGCCAC
TGTAGCCCGGGTTTGCACATCGGTTCCCTTCTACCGGCAGAGCTTTGACCGGGCCGGCATCACCCCCGACAGTGTCAAGT
CCCTGGCCGATCTGCAACGGCTCCCCTTCACCCTAAAGCAGAACATGCGCGACAGCTACCCCTATGGCCTCTTTGCGGCG
CCCATGGAGGAGATTGTCCGCATCCATGCTTCCTCCGGCACCACCGGTAAGCCGACCGTGGTCGGCTACACCCGCAAGGA
CATCGATACCTGGTCCGAGCTCATGGCCCGCTCCTACGCCGCCGCCGGGGTCCACCGGGGGGACATCATCCATAACTCCT
ACGGCTACGGCCTCTTCACCGGCGGCCTTGGCGCCCACTATGGCGCGGAGAAGCTCGGCGCTTCAGTCATCCCCATGTCG
GGGGGGAACACCAAGAAGCAGATTTTAATCATGAAGGATTTCGGTTCCACGGTCCTCACCTGCACCCCGTCATATTCGCT
CTTCATGTCCGAGGCCGCACGGGAGGAAGGAGTGGATTTCCGCGAGTTGAAGCTCCGCGTCGGTATCTTCGGGGCAGAAC
CGTGGTCAGAGGCGATGCGGAGCGAGATTGAGGAGAAGCTGAACCTCTGCGCCATCGACATCTACGGCCTGTCGGAAATA
ATGGGGCCGGGTGTGGCCATCGAATGCTGCGAGGCGAAGAAGGGGCTCCACATCTGGGAAGACCACTTCATCCCCGAAAT
CATCAATCCCGAGACCGGTGAGGTTCTTCCGGAAGGGGAGCGAGGCGAACTGGTCATCACCACTATCACCAAGGAGGGGA
TCCCCCTCATCCGGTACCGTACCCGCGACATCACAAGCCTCACGTACGAGCCGTGTGCCTGCGGCAGGACCCACGCCCGC
CTTACCCGTATGACCGGCAGAAGCGATGACATGCTCATTATCCGCGGAGTCAACGTGTTCCCGTCGCAGATTGAGTCAAT
CCTCATGGGAATCGAGGGGGTCGAACCCCACTATGTTCTCATTGTCGACCGCAAGGATAACCTGGATTCCCTTGAGGTTC
AGGTGGAAGTGGACGAACGGCTCTTCTCCGATGAGATCAAGGTGCTCCAGTCCCTGTCCCAACGGGTCGAAAAGGAGATA
AAGGACATTCTTGGCATATCATGCCGGGTCCGTCTTGTGGAACCCCAGACCATAACCCGGAGTGAAGGCAAGGCGAAGCG
GGTCATCGACAATCGGAAAAGCAGCTGA

Upstream 100 bases:

>100_bases
GATCAAGGCCCTGGAAAAGATGATTCCACCGAAGGCCCTCAAAGTTAACCATGTTGCGTTCCAACTGGGCCGGAGTCTTT
GATCGGGTAAGGAGTTCACG

Downstream 100 bases:

>100_bases
TGCCCGCCGGGGCACACTCGTGCCGCGTCCAAAACAAGGAGAGCGAGCAGGCCTGACCCTGCGAGGAGGATGGAATGAAC
GTTGAGCAGATCTCAATTTT

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSYYNEEFETLPRQALEALQLKRLQATVARVCTSVPFYRQSFDRAGITPDSVKSLADLQRLPFTLKQNMRDSYPYGLFAA
PMEEIVRIHASSGTTGKPTVVGYTRKDIDTWSELMARSYAAAGVHRGDIIHNSYGYGLFTGGLGAHYGAEKLGASVIPMS
GGNTKKQILIMKDFGSTVLTCTPSYSLFMSEAAREEGVDFRELKLRVGIFGAEPWSEAMRSEIEEKLNLCAIDIYGLSEI
MGPGVAIECCEAKKGLHIWEDHFIPEIINPETGEVLPEGERGELVITTITKEGIPLIRYRTRDITSLTYEPCACGRTHAR
LTRMTGRSDDMLIIRGVNVFPSQIESILMGIEGVEPHYVLIVDRKDNLDSLEVQVEVDERLFSDEIKVLQSLSQRVEKEI
KDILGISCRVRLVEPQTITRSEGKAKRVIDNRKSS

Sequences:

>Translated_435_residues
MSYYNEEFETLPRQALEALQLKRLQATVARVCTSVPFYRQSFDRAGITPDSVKSLADLQRLPFTLKQNMRDSYPYGLFAA
PMEEIVRIHASSGTTGKPTVVGYTRKDIDTWSELMARSYAAAGVHRGDIIHNSYGYGLFTGGLGAHYGAEKLGASVIPMS
GGNTKKQILIMKDFGSTVLTCTPSYSLFMSEAAREEGVDFRELKLRVGIFGAEPWSEAMRSEIEEKLNLCAIDIYGLSEI
MGPGVAIECCEAKKGLHIWEDHFIPEIINPETGEVLPEGERGELVITTITKEGIPLIRYRTRDITSLTYEPCACGRTHAR
LTRMTGRSDDMLIIRGVNVFPSQIESILMGIEGVEPHYVLIVDRKDNLDSLEVQVEVDERLFSDEIKVLQSLSQRVEKEI
KDILGISCRVRLVEPQTITRSEGKAKRVIDNRKSS
>Mature_434_residues
SYYNEEFETLPRQALEALQLKRLQATVARVCTSVPFYRQSFDRAGITPDSVKSLADLQRLPFTLKQNMRDSYPYGLFAAP
MEEIVRIHASSGTTGKPTVVGYTRKDIDTWSELMARSYAAAGVHRGDIIHNSYGYGLFTGGLGAHYGAEKLGASVIPMSG
GNTKKQILIMKDFGSTVLTCTPSYSLFMSEAAREEGVDFRELKLRVGIFGAEPWSEAMRSEIEEKLNLCAIDIYGLSEIM
GPGVAIECCEAKKGLHIWEDHFIPEIINPETGEVLPEGERGELVITTITKEGIPLIRYRTRDITSLTYEPCACGRTHARL
TRMTGRSDDMLIIRGVNVFPSQIESILMGIEGVEPHYVLIVDRKDNLDSLEVQVEVDERLFSDEIKVLQSLSQRVEKEIK
DILGISCRVRLVEPQTITRSEGKAKRVIDNRKSS

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=429, Percent_Identity=54.0792540792541, Blast_Score=467, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48626; Mature: 48495

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYYNEEFETLPRQALEALQLKRLQATVARVCTSVPFYRQSFDRAGITPDSVKSLADLQR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
LPFTLKQNMRDSYPYGLFAAPMEEIVRIHASSGTTGKPTVVGYTRKDIDTWSELMARSYA
CCHHHHHCCCCCCCCCEEHHHHHHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
AAGVHRGDIIHNSYGYGLFTGGLGAHYGAEKLGASVIPMSGGNTKKQILIMKDFGSTVLT
HCCCCCCCEEECCCCCEEEECCCCCCCCHHHHCCEEEECCCCCCCEEEEEEECCCCEEEE
CTPSYSLFMSEAAREEGVDFRELKLRVGIFGAEPWSEAMRSEIEEKLNLCAIDIYGLSEI
ECCCHHHHHHHHHHHHCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCHHHH
MGPGVAIECCEAKKGLHIWEDHFIPEIINPETGEVLPEGERGELVITTITKEGIPLIRYR
CCCCCEEEEECCCCCCEEHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEE
TRDITSLTYEPCACGRTHARLTRMTGRSDDMLIIRGVNVFPSQIESILMGIEGVEPHYVL
CCCCCEECCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEE
IVDRKDNLDSLEVQVEVDERLFSDEIKVLQSLSQRVEKEIKDILGISCRVRLVEPQTITR
EEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
SEGKAKRVIDNRKSS
CCCCHHHHHHCCCCC
>Mature Secondary Structure 
SYYNEEFETLPRQALEALQLKRLQATVARVCTSVPFYRQSFDRAGITPDSVKSLADLQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
LPFTLKQNMRDSYPYGLFAAPMEEIVRIHASSGTTGKPTVVGYTRKDIDTWSELMARSYA
CCHHHHHCCCCCCCCCEEHHHHHHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
AAGVHRGDIIHNSYGYGLFTGGLGAHYGAEKLGASVIPMSGGNTKKQILIMKDFGSTVLT
HCCCCCCCEEECCCCCEEEECCCCCCCCHHHHCCEEEECCCCCCCEEEEEEECCCCEEEE
CTPSYSLFMSEAAREEGVDFRELKLRVGIFGAEPWSEAMRSEIEEKLNLCAIDIYGLSEI
ECCCHHHHHHHHHHHHCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCHHHH
MGPGVAIECCEAKKGLHIWEDHFIPEIINPETGEVLPEGERGELVITTITKEGIPLIRYR
CCCCCEEEEECCCCCCEEHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEE
TRDITSLTYEPCACGRTHARLTRMTGRSDDMLIIRGVNVFPSQIESILMGIEGVEPHYVL
CCCCCEECCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEE
IVDRKDNLDSLEVQVEVDERLFSDEIKVLQSLSQRVEKEIKDILGISCRVRLVEPQTITR
EEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
SEGKAKRVIDNRKSS
CCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]