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The map label for this gene is paaK [H]

Identifier: 78223025

GI number: 78223025

Start: 2032014

End: 2033315

Strand: Reverse

Name: paaK [H]

Synonym: Gmet_1818

Alternate gene names: 78223025

Gene position: 2033315-2032014 (Counterclockwise)

Preceding gene: 78223026

Following gene: 78223024

Centisome position: 50.87

GC content: 58.6

Gene sequence:

>1302_bases
ATGCAGATCTGGGAACCAACATACGAATGCATGCCGCGCGAGGAGCTGCAGCAGCTTCAGCTGGAACGGCTCCAGGCAAC
CATCAACCGCGTATACAAGAATGTCACCTGCTACCGGAACAAGTTCAACGAGCTTGGTATCGTCCCTGAGGACATCCAGT
CCCTCGACGACCTCAGGAAACTCCCTTTCACTACCAAGGAGGACTTACGCCTGAACTATCCCTACGGGATGTTCGCCGTG
CCGCTCCGGGAGGTCGTGCGGATCCACTCCTCGTCGGGAACCACCGGCAAGCCCACGGTGGTCGGTTACACCAAGCACGA
CATCAAGGTCTGGTCGAATCTGGTGGCCCGCTTCATGACCGCCGCCGGCGTCACCCTCGACGACGTGGTTCAGGTCGCCT
TTGGTTACGGTCTCTTTACGGGCGCTTTTGGCCTCCATTACGGGACTGAAACCATCGGGGCGTCGGTGATTCCCATGAGC
TCCGGGAACACCGAAAAGCAGATCATGATCATGCAGGACTACAAGACCACGGCGCTGGTCAGCACCCCCAGCTATGCCAT
CACCATCGCGGACCGGATGGAAAAGATGGGAATCGATCCCAAGACCCTGTCGCTCAAGGTGGGGCTCTTCGGCGGTGAGC
CATGGTCAGAAGCGATGCGGAAGGAAATCGAAAGCAGACTTTTCATCAGTGCTACCGACAATTACGGTCTCTCGGAAGTT
ATTGGCCCCGGCATTGCCGGCGACTGTGAACACAAGTGCGGCATGCACATCTTCGAGGATGCCTTCATCCCCGAGATCAT
CGATCCGGATACCGGCGAGGTGTTGCCGCCGGGGAGCGTCGGTGAACTGGTGCTGACCACCCTCACCAAGGAGGCGTTCC
CCATGATCCGTTACCGGACCCGGGACATCACGTGCCTCGATTACTCCCCCTGCGCCTGTGGCAGGACCATGGTCCGCATG
AAGAAGACCATGGGGCGCAGCGATGATATGCTCATCATCAAGGGGGTCAACGTCTTCCCGTCCCAGATCGAGGAGGTTCT
CTTTGCCATCGAAGGGTGCCAACCCCACTACCAGATCATCGTTGACCGCAAGGGGACCCTCGATACCCTGGAGATCCAGA
TCGAGGTCATCGAAAACATCTTCTTCGACGAGATGAAGCGCCAGCGTGCGTTCCTCGAAATGGTCGAGAAGCGGATCGAT
TCGAGGCTCGGCGTCGGCGCCACCGTCAAGCTTGTGGAGCCGAACAGTATTCCCCGCCATGAAGGGAAAGCCCAGCGGGT
CATTGACCGGCGCAAGCTCTGA

Upstream 100 bases:

>100_bases
GAAGGAATTGCCTCTGAGTTGTTGGAAAACAAGGAGGTTCAGAGGGCTTACCTCGGGAAAGACAAGAAGGAAATCTGGGA
AAGATAGGCGAGGTCACCGT

Downstream 100 bases:

>100_bases
ACAATCCTATCCACCATTCAGCAAACATTCAAGGAGTTCCCATGCCAACAGCAACCGCCCGTCACATCCTCGTGTCCACC
GAAGCCGAATGCCTGCAACT

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MQIWEPTYECMPREELQQLQLERLQATINRVYKNVTCYRNKFNELGIVPEDIQSLDDLRKLPFTTKEDLRLNYPYGMFAV
PLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMTAAGVTLDDVVQVAFGYGLFTGAFGLHYGTETIGASVIPMS
SGNTEKQIMIMQDYKTTALVSTPSYAITIADRMEKMGIDPKTLSLKVGLFGGEPWSEAMRKEIESRLFISATDNYGLSEV
IGPGIAGDCEHKCGMHIFEDAFIPEIIDPDTGEVLPPGSVGELVLTTLTKEAFPMIRYRTRDITCLDYSPCACGRTMVRM
KKTMGRSDDMLIIKGVNVFPSQIEEVLFAIEGCQPHYQIIVDRKGTLDTLEIQIEVIENIFFDEMKRQRAFLEMVEKRID
SRLGVGATVKLVEPNSIPRHEGKAQRVIDRRKL

Sequences:

>Translated_433_residues
MQIWEPTYECMPREELQQLQLERLQATINRVYKNVTCYRNKFNELGIVPEDIQSLDDLRKLPFTTKEDLRLNYPYGMFAV
PLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMTAAGVTLDDVVQVAFGYGLFTGAFGLHYGTETIGASVIPMS
SGNTEKQIMIMQDYKTTALVSTPSYAITIADRMEKMGIDPKTLSLKVGLFGGEPWSEAMRKEIESRLFISATDNYGLSEV
IGPGIAGDCEHKCGMHIFEDAFIPEIIDPDTGEVLPPGSVGELVLTTLTKEAFPMIRYRTRDITCLDYSPCACGRTMVRM
KKTMGRSDDMLIIKGVNVFPSQIEEVLFAIEGCQPHYQIIVDRKGTLDTLEIQIEVIENIFFDEMKRQRAFLEMVEKRID
SRLGVGATVKLVEPNSIPRHEGKAQRVIDRRKL
>Mature_433_residues
MQIWEPTYECMPREELQQLQLERLQATINRVYKNVTCYRNKFNELGIVPEDIQSLDDLRKLPFTTKEDLRLNYPYGMFAV
PLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMTAAGVTLDDVVQVAFGYGLFTGAFGLHYGTETIGASVIPMS
SGNTEKQIMIMQDYKTTALVSTPSYAITIADRMEKMGIDPKTLSLKVGLFGGEPWSEAMRKEIESRLFISATDNYGLSEV
IGPGIAGDCEHKCGMHIFEDAFIPEIIDPDTGEVLPPGSVGELVLTTLTKEAFPMIRYRTRDITCLDYSPCACGRTMVRM
KKTMGRSDDMLIIKGVNVFPSQIEEVLFAIEGCQPHYQIIVDRKGTLDTLEIQIEVIENIFFDEMKRQRAFLEMVEKRID
SRLGVGATVKLVEPNSIPRHEGKAQRVIDRRKL

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=422, Percent_Identity=52.3696682464455, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48802; Mature: 48802

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIWEPTYECMPREELQQLQLERLQATINRVYKNVTCYRNKFNELGIVPEDIQSLDDLRK
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LPFTTKEDLRLNYPYGMFAVPLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMT
CCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
AAGVTLDDVVQVAFGYGLFTGAFGLHYGTETIGASVIPMSSGNTEKQIMIMQDYKTTALV
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCEEEEEECCCCEEEE
STPSYAITIADRMEKMGIDPKTLSLKVGLFGGEPWSEAMRKEIESRLFISATDNYGLSEV
ECCCEEEEEHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHCEEEEECCCCCHHHH
IGPGIAGDCEHKCGMHIFEDAFIPEIIDPDTGEVLPPGSVGELVLTTLTKEAFPMIRYRT
HCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEC
RDITCLDYSPCACGRTMVRMKKTMGRSDDMLIIKGVNVFPSQIEEVLFAIEGCQPHYQII
CEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
VDRKGTLDTLEIQIEVIENIFFDEMKRQRAFLEMVEKRIDSRLGVGATVKLVEPNSIPRH
EECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
EGKAQRVIDRRKL
CCHHHHHHHHCCC
>Mature Secondary Structure
MQIWEPTYECMPREELQQLQLERLQATINRVYKNVTCYRNKFNELGIVPEDIQSLDDLRK
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LPFTTKEDLRLNYPYGMFAVPLREVVRIHSSSGTTGKPTVVGYTKHDIKVWSNLVARFMT
CCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
AAGVTLDDVVQVAFGYGLFTGAFGLHYGTETIGASVIPMSSGNTEKQIMIMQDYKTTALV
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCEEEEEECCCCEEEE
STPSYAITIADRMEKMGIDPKTLSLKVGLFGGEPWSEAMRKEIESRLFISATDNYGLSEV
ECCCEEEEEHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHCEEEEECCCCCHHHH
IGPGIAGDCEHKCGMHIFEDAFIPEIIDPDTGEVLPPGSVGELVLTTLTKEAFPMIRYRT
HCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEC
RDITCLDYSPCACGRTMVRMKKTMGRSDDMLIIKGVNVFPSQIEEVLFAIEGCQPHYQII
CEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
VDRKGTLDTLEIQIEVIENIFFDEMKRQRAFLEMVEKRIDSRLGVGATVKLVEPNSIPRH
EECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
EGKAQRVIDRRKL
CCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]