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The map label for this gene is greA

Identifier: 78222984

GI number: 78222984

Start: 1992067

End: 1992546

Strand: Direct

Name: greA

Synonym: Gmet_1775

Alternate gene names: 78222984

Gene position: 1992067-1992546 (Clockwise)

Preceding gene: 78222983

Following gene: 78222985

Centisome position: 49.83

GC content: 58.33

Gene sequence:

>480_bases
ATGTCCTATTCAGTACCCATGACAAAAGAGAGTTACGATTCCCTTCAGGAAGAGCTCAAGCGTCTGATCCGCGAGGAGCG
GCCCCGCGTGATCCAGGATATTGCCGAGGCCCGCAGCCACGGCGACCTTTCGGAGAACGCCGAGTACGACGCCGCCAAGA
ACCGCCAGGCGTTCATCGAGGGACGCATTCAGGAACTCCAGGGAAAGCTTGCCCGTGCCCACGTGGTTGATCTTTCCGGC
CTGAAGCCCGACAAGGTGGTTTTCGGCGCCACCGTCACCCTCTACGACACCACCAGCGAGGAAGAGGTAACCTACAAGAT
CGTCGGCGAAGAGGAGGCCGACATCAAGCTGGGGAAGATCTCGTGCACCTCGCCGGTCGGCAAGGCCCTCATCGGCCACA
AGCTCGACGACAGCGTCAAAATCAAGGTGCCGTCGGGGTCCGTTAAGGAATACGAGATCATCGACATCAAATATATCTGA

Upstream 100 bases:

>100_bases
TGCCGAAGGAGGCTACTCTCCGGAATTCAATCTGGCGGAATGTACCAACGGGGCGGCCAAACGGCTGCCCCGCGATTTTC
ATAGAGGAGCAGTGAGTACG

Downstream 100 bases:

>100_bases
TTTTTCGTCACCAACCGTTCAACAAGGAAAGGAAATGCAATGAGCCAGCAATTCACCAAGGATATGACGTTCGCCCAAGC
CCTCCAGGCCAACCCCGAAG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSYSVPMTKESYDSLQEELKRLIREERPRVIQDIAEARSHGDLSENAEYDAAKNRQAFIEGRIQELQGKLARAHVVDLSG
LKPDKVVFGATVTLYDTTSEEEVTYKIVGEEEADIKLGKISCTSPVGKALIGHKLDDSVKIKVPSGSVKEYEIIDIKYI

Sequences:

>Translated_159_residues
MSYSVPMTKESYDSLQEELKRLIREERPRVIQDIAEARSHGDLSENAEYDAAKNRQAFIEGRIQELQGKLARAHVVDLSG
LKPDKVVFGATVTLYDTTSEEEVTYKIVGEEEADIKLGKISCTSPVGKALIGHKLDDSVKIKVPSGSVKEYEIIDIKYI
>Mature_158_residues
SYSVPMTKESYDSLQEELKRLIREERPRVIQDIAEARSHGDLSENAEYDAAKNRQAFIEGRIQELQGKLARAHVVDLSGL
KPDKVVFGATVTLYDTTSEEEVTYKIVGEEEADIKLGKISCTSPVGKALIGHKLDDSVKIKVPSGSVKEYEIIDIKYI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=48.4076433121019, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI87082261, Length=147, Percent_Identity=36.0544217687075, Blast_Score=84, Evalue=6e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17771; Mature: 17640

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSVPMTKESYDSLQEELKRLIREERPRVIQDIAEARSHGDLSENAEYDAAKNRQAFIE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
GRIQELQGKLARAHVVDLSGLKPDKVVFGATVTLYDTTSEEEVTYKIVGEEEADIKLGKI
HHHHHHHHHHHHHEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCCCCEEEEEE
SCTSPVGKALIGHKLDDSVKIKVPSGSVKEYEIIDIKYI
ECCCHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEEC
>Mature Secondary Structure 
SYSVPMTKESYDSLQEELKRLIREERPRVIQDIAEARSHGDLSENAEYDAAKNRQAFIE
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
GRIQELQGKLARAHVVDLSGLKPDKVVFGATVTLYDTTSEEEVTYKIVGEEEADIKLGKI
HHHHHHHHHHHHHEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCCCCEEEEEE
SCTSPVGKALIGHKLDDSVKIKVPSGSVKEYEIIDIKYI
ECCCHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA