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The map label for this gene is rpfC [H]

Identifier: 78222974

GI number: 78222974

Start: 1979036

End: 1980424

Strand: Reverse

Name: rpfC [H]

Synonym: Gmet_1765

Alternate gene names: 78222974

Gene position: 1980424-1979036 (Counterclockwise)

Preceding gene: 78222988

Following gene: 78222973

Centisome position: 49.54

GC content: 53.2

Gene sequence:

>1389_bases
ATGTCAAGATTAGGCGTCATTATTGATTTGGCGATTTGCTGCCACCGGCATAAAGAGCCGGCTCATCCATTACAAGCTAA
ATACAGTGCGTCGAGAGGAACACCGACCGTGACCCAGACCGGAACGACAGCCGTAACGGATGCCGATCGAATCGTTGAAC
TCGAACATCTTCTCGTCGAATTGAAGGCGGAACTGGCCGAGAGGAAAAATATCGAACGGGATCTCAAGCGAGCCAAGGTT
TCAGCCGAAGCTGCCTGCCACTCAAAAGAAGATCTTCTCGCCCTGGTGAGTCATGAAATCCGCTCTCAAATACAAATGCT
GACCGGTGCCGTCGAATTGCTCCGCGAAACCCCCCTCGACAAGAAACAGACTGAATACCTGAATGCCTTCAATCATGCAG
GCGAATTTCTTCTTTCTCTTGTCAATGATCTTCTTGACAACTCACGGCTTGACGCAGGACGTATTCACCTTGACCAAATT
GAATTTCCGTTTCGGGAACTGCTCGAAAGAACGGCACAACTTATCGCCTGGCAGGCGGGCAGAAAAGGTCTTGAACTGGA
ATATCACCTGTCACCCGACATTCCTGCCCAACTTGTCGGTGATCCGAAGCGCCTTCAGCAGGTGATCCTCAATCTGGCCG
GTAATGCCATCAAGTTTACCGAACAGGGGAAGATCATCATCACCGTTGAACCGGAAGCATCATCGTTGGCTTCCCAGGAA
ATGCAGCTCATCTTCACCATTCAGGATACGGGGATCGGCATTCCCGGTGACAAGCTCGAAGAAATTTTCGAGCGATACGC
CCAGGCATCTCCGGCTACGTTCCGCACCTATGGAGGAACAGGGCTTGGACTAAACATCGCCAAGAGGCTTGTCGGCTTGA
TGGGGGGCACGATCAGAGTGACGAGCAAGGAGGGAAAGGGAACTTCTTTCGTGTTCAACGCCCGACTGGGAGTTACGCCC
CCCCTTCTCTCCTCCGCAGTAGAACGCAGAAAATCAAAGAGTCTGCCGGAACGGAGCCTTCGGATTCTTCTGGCAGAAGA
CGCCGCGGAAATACGAATGCTTCTCGGCGCCTTTCTCGGAACCACCTCCCATGCCGTCGATATGGCCCATAACGGAGAAG
AGGCGTTGAAGAAGTTCACTTCCAGCAACTATGACGTAATTATCATGGACATTCAGATGCCGGTCATGGATGGCCTTTCG
GCCACGAGGAAGATCCGGGAATGGGAGCGAACGCATGACCGGACGCCGGTGCCCGTCGTTGCTCTCACGGCCGGTCCTGA
ACGCAGCGATTATCTCAGGGCACGGGAAGCCGGTTGCACAACGCACCTTGCCAAACCGATCAAAAAGGAACTGCTCCTGA
ACACGATCAGCGCTGTGGTGCTGCAGTAA

Upstream 100 bases:

>100_bases
CGAAAGAGGGAGATTTTGTAAAGGGTATTCTCCGGATACATCCAGCATTAATTCTCCGCCACTTCTGCGATTGCCGAGCC
CCTGGAGCTGTGATATACAA

Downstream 100 bases:

>100_bases
CCAGAGGGCTCATGCCTTGGCCCGGGCCGGCATTAGCCGGAATCAAGGAATTAACGAGCGTACCTGCGTTGCCTTGCCGT
TTGATTCATGCTATACGTCC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSRLGVIIDLAICCHRHKEPAHPLQAKYSASRGTPTVTQTGTTAVTDADRIVELEHLLVELKAELAERKNIERDLKRAKV
SAEAACHSKEDLLALVSHEIRSQIQMLTGAVELLRETPLDKKQTEYLNAFNHAGEFLLSLVNDLLDNSRLDAGRIHLDQI
EFPFRELLERTAQLIAWQAGRKGLELEYHLSPDIPAQLVGDPKRLQQVILNLAGNAIKFTEQGKIIITVEPEASSLASQE
MQLIFTIQDTGIGIPGDKLEEIFERYAQASPATFRTYGGTGLGLNIAKRLVGLMGGTIRVTSKEGKGTSFVFNARLGVTP
PLLSSAVERRKSKSLPERSLRILLAEDAAEIRMLLGAFLGTTSHAVDMAHNGEEALKKFTSSNYDVIIMDIQMPVMDGLS
ATRKIREWERTHDRTPVPVVALTAGPERSDYLRAREAGCTTHLAKPIKKELLLNTISAVVLQ

Sequences:

>Translated_462_residues
MSRLGVIIDLAICCHRHKEPAHPLQAKYSASRGTPTVTQTGTTAVTDADRIVELEHLLVELKAELAERKNIERDLKRAKV
SAEAACHSKEDLLALVSHEIRSQIQMLTGAVELLRETPLDKKQTEYLNAFNHAGEFLLSLVNDLLDNSRLDAGRIHLDQI
EFPFRELLERTAQLIAWQAGRKGLELEYHLSPDIPAQLVGDPKRLQQVILNLAGNAIKFTEQGKIIITVEPEASSLASQE
MQLIFTIQDTGIGIPGDKLEEIFERYAQASPATFRTYGGTGLGLNIAKRLVGLMGGTIRVTSKEGKGTSFVFNARLGVTP
PLLSSAVERRKSKSLPERSLRILLAEDAAEIRMLLGAFLGTTSHAVDMAHNGEEALKKFTSSNYDVIIMDIQMPVMDGLS
ATRKIREWERTHDRTPVPVVALTAGPERSDYLRAREAGCTTHLAKPIKKELLLNTISAVVLQ
>Mature_461_residues
SRLGVIIDLAICCHRHKEPAHPLQAKYSASRGTPTVTQTGTTAVTDADRIVELEHLLVELKAELAERKNIERDLKRAKVS
AEAACHSKEDLLALVSHEIRSQIQMLTGAVELLRETPLDKKQTEYLNAFNHAGEFLLSLVNDLLDNSRLDAGRIHLDQIE
FPFRELLERTAQLIAWQAGRKGLELEYHLSPDIPAQLVGDPKRLQQVILNLAGNAIKFTEQGKIIITVEPEASSLASQEM
QLIFTIQDTGIGIPGDKLEEIFERYAQASPATFRTYGGTGLGLNIAKRLVGLMGGTIRVTSKEGKGTSFVFNARLGVTPP
LLSSAVERRKSKSLPERSLRILLAEDAAEIRMLLGAFLGTTSHAVDMAHNGEEALKKFTSSNYDVIIMDIQMPVMDGLSA
TRKIREWERTHDRTPVPVVALTAGPERSDYLRAREAGCTTHLAKPIKKELLLNTISAVVLQ

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=260, Percent_Identity=41.1538461538462, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI48994928, Length=380, Percent_Identity=31.8421052631579, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081816, Length=354, Percent_Identity=32.2033898305085, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788713, Length=392, Percent_Identity=30.3571428571429, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=33.984375, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=29.2887029288703, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=28.82096069869, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788393, Length=232, Percent_Identity=25.8620689655172, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788549, Length=256, Percent_Identity=28.125, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786912, Length=271, Percent_Identity=26.1992619926199, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787894, Length=250, Percent_Identity=27.6, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790300, Length=248, Percent_Identity=27.8225806451613, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786783, Length=313, Percent_Identity=24.2811501597444, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790861, Length=248, Percent_Identity=27.8225806451613, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790346, Length=223, Percent_Identity=27.3542600896861, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788550, Length=123, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323034, Length=185, Percent_Identity=25.9459459459459, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50994; Mature: 50863

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLGVIIDLAICCHRHKEPAHPLQAKYSASRGTPTVTQTGTTAVTDADRIVELEHLLVE
CCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEECCCEEECCHHHHHHHHHHHHH
LKAELAERKNIERDLKRAKVSAEAACHSKEDLLALVSHEIRSQIQMLTGAVELLRETPLD
HHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KKQTEYLNAFNHAGEFLLSLVNDLLDNSRLDAGRIHLDQIEFPFRELLERTAQLIAWQAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHCCCHHHHHHHHHHHHHHHHCC
RKGLELEYHLSPDIPAQLVGDPKRLQQVILNLAGNAIKFTEQGKIIITVEPEASSLASQE
CCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECCCHHHHHHCC
MQLIFTIQDTGIGIPGDKLEEIFERYAQASPATFRTYGGTGLGLNIAKRLVGLMGGTIRV
EEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEE
TSKEGKGTSFVFNARLGVTPPLLSSAVERRKSKSLPERSLRILLAEDAAEIRMLLGAFLG
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHEEEECCCHHHHHHHHHHHHC
TTSHAVDMAHNGEEALKKFTSSNYDVIIMDIQMPVMDGLSATRKIREWERTHDRTPVPVV
CCHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEE
ALTAGPERSDYLRAREAGCTTHLAKPIKKELLLNTISAVVLQ
EEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLGVIIDLAICCHRHKEPAHPLQAKYSASRGTPTVTQTGTTAVTDADRIVELEHLLVE
CCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEECCCEEECCHHHHHHHHHHHHH
LKAELAERKNIERDLKRAKVSAEAACHSKEDLLALVSHEIRSQIQMLTGAVELLRETPLD
HHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KKQTEYLNAFNHAGEFLLSLVNDLLDNSRLDAGRIHLDQIEFPFRELLERTAQLIAWQAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHCCCHHHHHHHHHHHHHHHHCC
RKGLELEYHLSPDIPAQLVGDPKRLQQVILNLAGNAIKFTEQGKIIITVEPEASSLASQE
CCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECCCHHHHHHCC
MQLIFTIQDTGIGIPGDKLEEIFERYAQASPATFRTYGGTGLGLNIAKRLVGLMGGTIRV
EEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEE
TSKEGKGTSFVFNARLGVTPPLLSSAVERRKSKSLPERSLRILLAEDAAEIRMLLGAFLG
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHEEEECCCHHHHHHHHHHHHC
TTSHAVDMAHNGEEALKKFTSSNYDVIIMDIQMPVMDGLSATRKIREWERTHDRTPVPVV
CCHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEE
ALTAGPERSDYLRAREAGCTTHLAKPIKKELLLNTISAVVLQ
EEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]