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The map label for this gene is panB

Identifier: 78222851

GI number: 78222851

Start: 1843294

End: 1844097

Strand: Direct

Name: panB

Synonym: Gmet_1642

Alternate gene names: 78222851

Gene position: 1843294-1844097 (Clockwise)

Preceding gene: 78222850

Following gene: 78222852

Centisome position: 46.11

GC content: 57.84

Gene sequence:

>804_bases
GTGAACCGCAAGAAGACAATTATTGACATCCAGAAGATGAAGGCAGCCGGCGAGAAGATAACCGTCCTCACCAGCTATGA
TTACCCCTTTACCCGCGTAATGGATGAATGCGGCATTGACATGATCCTCATCGGCGATTCCGTGGGCGTTGTCTTCAGTG
GGTATGACAACACCCTGCCGGTCACGATGGACGAGATGATCTATCACACCCGGGCGGTGGTGCGGGCCCGGCCCAAGGCC
CTGGTTGTGGCCGACATGCCGTTCCTCTCCTACCAGACCGACCTGCGGGACGCCCGTCTCAATGCCGGCCGTCTCGTGAA
GGATGGGGGGGCGGAAGCCGTCAAGCTGGAAGGTGGGAGCCATGTGGCCGATACCATCCGCGCCATTGTCGACATGGATA
TTCCGGTTATGGCCCATATCGGTCTCACCCCCCAGTCGATTCACCGGATGGGGGGGTACAAGGTCCAGGGGAAAAAGGAA
GAGCAGTCCCAGCGGCTTCTGGATGATGCCAAGGCCATTGAAGAAGCCGGCGCCTTTGCCGTGGTTCTGGAGGGGATCCC
GCTGAAACTGGCGGAAAAGATCACCGAAGAGCTTTCCATTCCCACAATCGGCATTGGCGCGGGGCCCCACTGCGACGGTC
AGGTGCTTGTGATCCATGACATCCTGGGACTGTGCGAGAAATACTCTCCAAAGTTCGTGAAACGTTACGGGGACGCCAAT
GCGCTGATAACCAGCGCGGTTTCCTCATACATCGCCGAAGTGAAGAAAGGAGAATTCCCCGACGAGGGGCATTCATTCAG
CTGA

Upstream 100 bases:

>100_bases
ATGGCAATAGCCGCAACGACAAGGAAATAGCTTTTTCTCTTGGTCTTGGTACATGCGCCTCCGGAACCCCGGTGGCGCAT
TTTTGTTGTGTGGAGCAACC

Downstream 100 bases:

>100_bases
GATGAGAATCATTGAAACCGTAGCCGAGATGCAGGCCTTTTCGCGGGAGGCACGAAGGGAGGGGAAAAGCATTTCCCTGG
TCCCGACCATGGGGTTCCTC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNRKKTIIDIQKMKAAGEKITVLTSYDYPFTRVMDECGIDMILIGDSVGVVFSGYDNTLPVTMDEMIYHTRAVVRARPKA
LVVADMPFLSYQTDLRDARLNAGRLVKDGGAEAVKLEGGSHVADTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKE
EQSQRLLDDAKAIEEAGAFAVVLEGIPLKLAEKITEELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAN
ALITSAVSSYIAEVKKGEFPDEGHSFS

Sequences:

>Translated_267_residues
MNRKKTIIDIQKMKAAGEKITVLTSYDYPFTRVMDECGIDMILIGDSVGVVFSGYDNTLPVTMDEMIYHTRAVVRARPKA
LVVADMPFLSYQTDLRDARLNAGRLVKDGGAEAVKLEGGSHVADTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKE
EQSQRLLDDAKAIEEAGAFAVVLEGIPLKLAEKITEELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAN
ALITSAVSSYIAEVKKGEFPDEGHSFS
>Mature_267_residues
MNRKKTIIDIQKMKAAGEKITVLTSYDYPFTRVMDECGIDMILIGDSVGVVFSGYDNTLPVTMDEMIYHTRAVVRARPKA
LVVADMPFLSYQTDLRDARLNAGRLVKDGGAEAVKLEGGSHVADTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKE
EQSQRLLDDAKAIEEAGAFAVVLEGIPLKLAEKITEELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAN
ALITSAVSSYIAEVKKGEFPDEGHSFS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=44.3181818181818, Blast_Score=236, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6319653, Length=272, Percent_Identity=39.3382352941176, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_GEOMG (Q39V51)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384598.1
- ProteinModelPortal:   Q39V51
- SMR:   Q39V51
- STRING:   Q39V51
- GeneID:   3740411
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1642
- NMPDR:   fig|269799.3.peg.1959
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   RANSHAF
- PhylomeDB:   Q39V51
- ProtClustDB:   PRK00311
- BioCyc:   GMET269799:GMET_1642-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29081; Mature: 29081

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: ACT_SITE 184-184 BINDING 85-85 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKKTIIDIQKMKAAGEKITVLTSYDYPFTRVMDECGIDMILIGDSVGVVFSGYDNTLP
CCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCEEEEECCCCEEEEECCCCCCC
VTMDEMIYHTRAVVRARPKALVVADMPFLSYQTDLRDARLNAGRLVKDGGAEAVKLEGGS
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEECCCCCEEEECCCC
HVADTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKEEQSQRLLDDAKAIEEAGAFA
HHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHCCCEE
VVLEGIPLKLAEKITEELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAN
EEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHCCCH
ALITSAVSSYIAEVKKGEFPDEGHSFS
HHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNRKKTIIDIQKMKAAGEKITVLTSYDYPFTRVMDECGIDMILIGDSVGVVFSGYDNTLP
CCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCEEEEECCCCEEEEECCCCCCC
VTMDEMIYHTRAVVRARPKALVVADMPFLSYQTDLRDARLNAGRLVKDGGAEAVKLEGGS
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEECCCCCEEEECCCC
HVADTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKEEQSQRLLDDAKAIEEAGAFA
HHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHCCCEE
VVLEGIPLKLAEKITEELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAN
EEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHCCCH
ALITSAVSSYIAEVKKGEFPDEGHSFS
HHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA