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The map label for this gene is bioD [C]

Identifier: 78222825

GI number: 78222825

Start: 1815801

End: 1816892

Strand: Reverse

Name: bioD [C]

Synonym: Gmet_1616

Alternate gene names: 78222825

Gene position: 1816892-1815801 (Counterclockwise)

Preceding gene: 78222828

Following gene: 78222824

Centisome position: 45.45

GC content: 58.06

Gene sequence:

>1092_bases
ATGGCGAAGAGAGTCTTCATCGGCGCGACGGGACAGAACTGCGGCAAGACCACCATGAGCGTATCGCTCATGCACCTGGC
ACGCCAGAAATACGACCGAGTGGGCTTCATCAAACCCATCGGCCCCAAGATCGAGGGATACAACGGCCTCACCGTCGACA
TGGACGCCATTCTCATGGCCAGAACCTTCGGGCTGGAGGAGGACCTTCCCCTCATGAACCCGGTTTCGCTCCACAAGAAC
TTCACCCGCGATTTCCTCAGCGGCAAGCTCGATTGCAATGTCCTCAAGACGAAGATTCTCGAAGCCTTTGAAATTCTCGA
CAAGAAATATGATTTCCTCATCATCGAAGGGGCCGGGCACGGCGGCGTCGGATCGGTCATCGGCCTCAGCAACGCCTGCG
TTGCCCATACCCTCAACGCTCCGGTCATCATCGTGACCGACAGCGGCATCGGAAGCTCCATCGACGCGGTTCACCTGAAC
TTGGCGCTCTACGAGAAAGAGGACGCCGATGTCCGGATGTTACTCGTCAACAAGCTGAGGGCCGACAAGCGGGAGTCGAT
TCTCAGCTTCCTCGGCAAAGGTTTCCCGGGACGCCCCCTCAAGATCAGCGGCGGCTTCAACTACTCCCCCATCCTGGCAA
ACCCTACCCTCTCCCACATCGGCAAACTGCTCAATCTTCCGGTCCACGGCGACCCCGACGGGCAAAGCCGCATCATCCAC
CACATTCACTTGGGGGCAGCCTCTGCCCAGCGGGTGGTGGACGCCCTGCAGGAATCGACCCTCATCGTCATAACCAGCTC
CCGCGACGAGCTCATCGTCACCCTCTCCTCCCTCTATCACATCCCAGCTTTCCGGGAAAAGATCGTGGGACTTGTCATCG
CCGGCCACATACCCGCTTCGGAAATAAGCCAGCAAATTTTGGACGACAGCAATATCCCCTATATCAGGGTCCACGATTCC
ACGGCCAATGTCTTCACCACCCTCATGGAGGACGTGGCAAAGATCACCGCCGAGGACCAGGAGAAACTGAACTGGATCAG
GGCCAACGCTGAGAACGAGATCGACTTCGAAGCGATCGACGCCCTTCTCTGA

Upstream 100 bases:

>100_bases
ATCTATAGTGTATACTCATCTTCACTTCGACAACGCTCGTTGTCGGGCAATAGGTACGTCTTGAATATCTGATGGAATTC
AATCACCGCGGGGGAAACGG

Downstream 100 bases:

>100_bases
AAGCATTTGAAACAGCCCCATGCCCTACCGTTATCTCCCCGACATCGCCACTGCGGACACCGCCTTCGAGGCAACCGGTG
CCACCCGGGAGGAGATGTTC

Product: cobyrinic acid a,c-diamide synthase family protein

Products: ADP; Dethiobiotin; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAKRVFIGATGQNCGKTTMSVSLMHLARQKYDRVGFIKPIGPKIEGYNGLTVDMDAILMARTFGLEEDLPLMNPVSLHKN
FTRDFLSGKLDCNVLKTKILEAFEILDKKYDFLIIEGAGHGGVGSVIGLSNACVAHTLNAPVIIVTDSGIGSSIDAVHLN
LALYEKEDADVRMLLVNKLRADKRESILSFLGKGFPGRPLKISGGFNYSPILANPTLSHIGKLLNLPVHGDPDGQSRIIH
HIHLGAASAQRVVDALQESTLIVITSSRDELIVTLSSLYHIPAFREKIVGLVIAGHIPASEISQQILDDSNIPYIRVHDS
TANVFTTLMEDVAKITAEDQEKLNWIRANAENEIDFEAIDALL

Sequences:

>Translated_363_residues
MAKRVFIGATGQNCGKTTMSVSLMHLARQKYDRVGFIKPIGPKIEGYNGLTVDMDAILMARTFGLEEDLPLMNPVSLHKN
FTRDFLSGKLDCNVLKTKILEAFEILDKKYDFLIIEGAGHGGVGSVIGLSNACVAHTLNAPVIIVTDSGIGSSIDAVHLN
LALYEKEDADVRMLLVNKLRADKRESILSFLGKGFPGRPLKISGGFNYSPILANPTLSHIGKLLNLPVHGDPDGQSRIIH
HIHLGAASAQRVVDALQESTLIVITSSRDELIVTLSSLYHIPAFREKIVGLVIAGHIPASEISQQILDDSNIPYIRVHDS
TANVFTTLMEDVAKITAEDQEKLNWIRANAENEIDFEAIDALL
>Mature_362_residues
AKRVFIGATGQNCGKTTMSVSLMHLARQKYDRVGFIKPIGPKIEGYNGLTVDMDAILMARTFGLEEDLPLMNPVSLHKNF
TRDFLSGKLDCNVLKTKILEAFEILDKKYDFLIIEGAGHGGVGSVIGLSNACVAHTLNAPVIIVTDSGIGSSIDAVHLNL
ALYEKEDADVRMLLVNKLRADKRESILSFLGKGFPGRPLKISGGFNYSPILANPTLSHIGKLLNLPVHGDPDGQSRIIHH
IHLGAASAQRVVDALQESTLIVITSSRDELIVTLSSLYHIPAFREKIVGLVIAGHIPASEISQQILDDSNIPYIRVHDST
ANVFTTLMEDVAKITAEDQEKLNWIRANAENEIDFEAIDALL

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0857

COG function: function code R; BioD-like N-terminal domain of phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To Synechocystis PCC 6803 slr0039 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010766 [H]

Pfam domain/function: PF07085 DRTGG [H]

EC number: 6.3.3.3 [C]

Molecular weight: Translated: 39608; Mature: 39477

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRVFIGATGQNCGKTTMSVSLMHLARQKYDRVGFIKPIGPKIEGYNGLTVDMDAILMA
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHH
RTFGLEEDLPLMNPVSLHKNFTRDFLSGKLDCNVLKTKILEAFEILDKKYDFLIIEGAGH
HHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCC
GGVGSVIGLSNACVAHTLNAPVIIVTDSGIGSSIDAVHLNLALYEKEDADVRMLLVNKLR
CCCCHHHCCCCCHHHEECCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCEEEEHHHHHC
ADKRESILSFLGKGFPGRPLKISGGFNYSPILANPTLSHIGKLLNLPVHGDPDGQSRIIH
CCHHHHHHHHHHCCCCCCCEEEECCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCEEE
HIHLGAASAQRVVDALQESTLIVITSSRDELIVTLSSLYHIPAFREKIVGLVIAGHIPAS
EEECCCHHHHHHHHHHHCCEEEEEECCCCCEEEEEHHHHCCHHHHHHHEEEEEECCCCHH
EISQQILDDSNIPYIRVHDSTANVFTTLMEDVAKITAEDQEKLNWIRANAENEIDFEAID
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHEECCCCCCCCHHHHH
ALL
HCC
>Mature Secondary Structure 
AKRVFIGATGQNCGKTTMSVSLMHLARQKYDRVGFIKPIGPKIEGYNGLTVDMDAILMA
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHH
RTFGLEEDLPLMNPVSLHKNFTRDFLSGKLDCNVLKTKILEAFEILDKKYDFLIIEGAGH
HHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCC
GGVGSVIGLSNACVAHTLNAPVIIVTDSGIGSSIDAVHLNLALYEKEDADVRMLLVNKLR
CCCCHHHCCCCCHHHEECCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCEEEEHHHHHC
ADKRESILSFLGKGFPGRPLKISGGFNYSPILANPTLSHIGKLLNLPVHGDPDGQSRIIH
CCHHHHHHHHHHCCCCCCCEEEECCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCEEE
HIHLGAASAQRVVDALQESTLIVITSSRDELIVTLSSLYHIPAFREKIVGLVIAGHIPAS
EEECCCHHHHHHHHHHHCCEEEEEECCCCCEEEEEHHHHCCHHHHHHHEEEEEECCCCHH
EISQQILDDSNIPYIRVHDSTANVFTTLMEDVAKITAEDQEKLNWIRANAENEIDFEAID
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHEECCCCCCCCHHHHH
ALL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {MgCl2}} 0.42 {ATP}} 10 {HCO3-}} 0.045 {7,8-diaminononanoate}} [C]

Substrates: ATP; CO2; 7,8-Diaminononanoate [C]

Specific reaction: ATP + CO2 + 7,8-Diaminononanoate <==> ADP + Dethiobiotin + (3) H+ + Phosphate [C]

General reaction: Carboxylation; Heteroatomic ring closure [C]

Inhibitor: Ag+; Cd2+; Co2+; Cu2+; Hg2+; Ni2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]