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The map label for this gene is nusA

Identifier: 78222794

GI number: 78222794

Start: 1785258

End: 1786403

Strand: Direct

Name: nusA

Synonym: Gmet_1584

Alternate gene names: 78222794

Gene position: 1785258-1786403 (Clockwise)

Preceding gene: 78222793

Following gene: 78222795

Centisome position: 44.66

GC content: 55.67

Gene sequence:

>1146_bases
GTGGAAACGACCTTCAACCTCAAGCACATTATTGATCAGATCGTGAAGGAAAAGGGGATAGACCGGGATATCGTGGTCGA
GGCCCTTGAGCAGGCGGTGCTGTCCGCCGCGAACAAAAAGTACCGCAACACACGCGATCTTGAGGCCCATTACAATCCCG
AGGTCGGTGAAGTTGAGCTTTTCGAGTTCGTCACCGTGGTTGACGAGGTTATGGACTCGTACAAGGAGATTGATCTTGAG
GAAGCCCGTGAAATCGACCCTGACGTGGAGGTGGGCGACTCCCTCGGTATGAAGATGGATGCCAGCGGTTTCACGCGGAT
TGCCGCCCAGACCGCAAAGCAGGTAATCATTCAGAAGGTGCGGGAAGCGGAGCGGGAAACCATTTTCAATGAGTTCAAGG
ATCGGATCGGCGAGTTGATCAACGGTGTGGTCCGTCGTTTCGAGAAGGGGGATCTCGTTGTTGATCTTGGTCGCGCCGAG
GCGCTTCTTTCCAACAAGGAGCAGGCACCGCGCGAGGTTTACCGTCAGGGTGACCGTATCAAGGCGCTTATCATGGATAT
CCGTATGACTCCCAAGGGTCCCCAGATCATTCTGTCCCGGACCCATCCCGGTGTGCTTGCCAAGCTCTTCGAAGCCGAAG
TTCCGGAGATCGCCGAGGGGATCGTTGAAATCAAGTCCGTCGTCCGCGAACCGGGAAGCCGGTCAAAAATTGCGGTTTAC
TCCCACGATTCTGATGTTGATCCGGTTGGTGCGTGCGTCGGAATGCGCGGATCGAGGGTTCAGAACGTTGTGTCCGAGCT
TCGTGGCGAGAAAATCGACATCATCCCGTGGTCTGAAGACCCGGCGCGGTTTGCCTGTAATGCGCTCCAGCCGGCTGCGG
TTTCCAAGGTCTACATCGACGAAGAAAATAAAGCGCTCGAAATCATTGTCGCCGACGATCAGCTTTCCCTTGCCATTGGC
AAGAAGGGGCAGAATGTTCGGCTTGCCGCAAAACTCACCGGCTGGAGAATCGACATCAAGAGTGAAACCCGTGCTGCCGA
GGCAGAACTCCTTGAGTTTTCTTCCTACGACGGTGCTACTGAGGAAGCGCCTCAGGAACCTGTTGAGGAGTCCGAGGCTC
AACCGCTGGAGCAGGGCGAAGAGTAG

Upstream 100 bases:

>100_bases
GGGGCAGATGGCTCGAATCCCCCTTGCCAAAATCGCAAAAGCCAACCTTGAATTCGAGTTCTAGATTAGAAAGATAAAGC
ACTTCCAAGGAGAGACGGAC

Downstream 100 bases:

>100_bases
GAGCCAATGGCGAGGAGCGAGCCTCGGCGAACATGTCTGGGATGTCGGGCTGAGAGGGGGAAGGACGAACTTCTTCGCTA
TGTCCTTGACCCCGAGGGAA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
METTFNLKHIIDQIVKEKGIDRDIVVEALEQAVLSAANKKYRNTRDLEAHYNPEVGEVELFEFVTVVDEVMDSYKEIDLE
EAREIDPDVEVGDSLGMKMDASGFTRIAAQTAKQVIIQKVREAERETIFNEFKDRIGELINGVVRRFEKGDLVVDLGRAE
ALLSNKEQAPREVYRQGDRIKALIMDIRMTPKGPQIILSRTHPGVLAKLFEAEVPEIAEGIVEIKSVVREPGSRSKIAVY
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDPARFACNALQPAAVSKVYIDEENKALEIIVADDQLSLAIG
KKGQNVRLAAKLTGWRIDIKSETRAAEAELLEFSSYDGATEEAPQEPVEESEAQPLEQGEE

Sequences:

>Translated_381_residues
METTFNLKHIIDQIVKEKGIDRDIVVEALEQAVLSAANKKYRNTRDLEAHYNPEVGEVELFEFVTVVDEVMDSYKEIDLE
EAREIDPDVEVGDSLGMKMDASGFTRIAAQTAKQVIIQKVREAERETIFNEFKDRIGELINGVVRRFEKGDLVVDLGRAE
ALLSNKEQAPREVYRQGDRIKALIMDIRMTPKGPQIILSRTHPGVLAKLFEAEVPEIAEGIVEIKSVVREPGSRSKIAVY
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDPARFACNALQPAAVSKVYIDEENKALEIIVADDQLSLAIG
KKGQNVRLAAKLTGWRIDIKSETRAAEAELLEFSSYDGATEEAPQEPVEESEAQPLEQGEE
>Mature_381_residues
METTFNLKHIIDQIVKEKGIDRDIVVEALEQAVLSAANKKYRNTRDLEAHYNPEVGEVELFEFVTVVDEVMDSYKEIDLE
EAREIDPDVEVGDSLGMKMDASGFTRIAAQTAKQVIIQKVREAERETIFNEFKDRIGELINGVVRRFEKGDLVVDLGRAE
ALLSNKEQAPREVYRQGDRIKALIMDIRMTPKGPQIILSRTHPGVLAKLFEAEVPEIAEGIVEIKSVVREPGSRSKIAVY
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDPARFACNALQPAAVSKVYIDEENKALEIIVADDQLSLAIG
KKGQNVRLAAKLTGWRIDIKSETRAAEAELLEFSSYDGATEEAPQEPVEESEAQPLEQGEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=355, Percent_Identity=42.2535211267606, Blast_Score=294, Evalue=5e-81,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 42457; Mature: 42457

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTFNLKHIIDQIVKEKGIDRDIVVEALEQAVLSAANKKYRNTRDLEAHYNPEVGEVEL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
FEFVTVVDEVMDSYKEIDLEEAREIDPDVEVGDSLGMKMDASGFTRIAAQTAKQVIIQKV
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHH
REAERETIFNEFKDRIGELINGVVRRFEKGDLVVDLGRAEALLSNKEQAPREVYRQGDRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCHHHHHHCCCHH
KALIMDIRMTPKGPQIILSRTHPGVLAKLFEAEVPEIAEGIVEIKSVVREPGSRSKIAVY
EEHHHHEEECCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDPARFACNALQPAAVSKVYID
ECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCHHHEEEEEC
EENKALEIIVADDQLSLAIGKKGQNVRLAAKLTGWRIDIKSETRAAEAELLEFSSYDGAT
CCCCEEEEEEECCCEEEEECCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCC
EEAPQEPVEESEAQPLEQGEE
CCCCCCCHHHHCCCCHHCCCC
>Mature Secondary Structure
METTFNLKHIIDQIVKEKGIDRDIVVEALEQAVLSAANKKYRNTRDLEAHYNPEVGEVEL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
FEFVTVVDEVMDSYKEIDLEEAREIDPDVEVGDSLGMKMDASGFTRIAAQTAKQVIIQKV
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHH
REAERETIFNEFKDRIGELINGVVRRFEKGDLVVDLGRAEALLSNKEQAPREVYRQGDRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCHHHHHHCCCHH
KALIMDIRMTPKGPQIILSRTHPGVLAKLFEAEVPEIAEGIVEIKSVVREPGSRSKIAVY
EEHHHHEEECCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDPARFACNALQPAAVSKVYID
ECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCHHHEEEEEC
EENKALEIIVADDQLSLAIGKKGQNVRLAAKLTGWRIDIKSETRAAEAELLEFSSYDGAT
CCCCEEEEEEECCCEEEEECCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCC
EEAPQEPVEESEAQPLEQGEE
CCCCCCCHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]