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The map label for this gene is bioB

Identifier: 78222792

GI number: 78222792

Start: 1783529

End: 1784518

Strand: Reverse

Name: bioB

Synonym: Gmet_1582

Alternate gene names: 78222792

Gene position: 1784518-1783529 (Counterclockwise)

Preceding gene: 78222816

Following gene: 78222791

Centisome position: 44.64

GC content: 58.38

Gene sequence:

>990_bases
ATGAACAAATCCCTCGAAACACTGGCTGGCAGGATTATTGCCGGCGAACAACTCTCAGTGGAAGAGTCCATCGCTCTCTC
GGCCTCTAGCGGAACTGATGCCTTTGCCCTCTTTCTGGCCGCAAGCCGGGTCAAGGAACACTTCCTCGGCAACGGCGTCG
ATCTCTGTTCCATCATTAACGCCAAATCGGGACGCTGCCCGGAAAATTGCGCCTTCTGCGCCCAGTCGGCCCACCACACC
ACTAACGCGCCCATCTACCCCCTTGTAGACGAAGACAAGTTAGTGGCCTGCGCCAAGGAGGCTGAAGCAGCCGGTTCCCG
CTGCTATGGGATAATCACGAGCGGCACAACGATCAAAAAGGGGGAAGAACTTGACCGTATCTGCCGGGCTGTGCGCCGCA
TAAGAGAAACTACTTCCATTGCTCCCTCCTGCTCGCTGGGCATCATCGACCATGAAACGGCAGTGACTCTCAGGGAGGCG
GGCGTCGAGACCTATCACCACAATCTGGAAACGTCCCGGAGCTTTTTCCCACATATCTGCACCACCCACGACTACGAAGA
GGATGTTGAGACGGTACGGGTAGCGAAGCAGGCTGGTCTTACCATATGCTGCGGTGGTATCTTCGGCCTTGGGGAGACGG
CAGCGCAACGGGTTGAGCTGGCCATGACCCTGCGGGAGCTTGACGTGGACTCGGTACCCCTCAATTTCCTTAACCCCATT
GAGGGGACCCGGCTTGCGGGCTCAAAGCTCATCTCACCCCTTGAGTGCCTGAAAACCATCGCGCTTTTCCGGCTCATCCT
TCCGACAAAGAAAATTGCCGTCTGCGGCGGCCGCGAGCAGAACCTGCGGGATCTGCAGTCGTGGATATTCTTCGCGGGCG
CGAGCGGAACCATGATCGGCAACTACCTGACCACCACCGGCCGCCCCGCCGAGCAGGACTGGCAGATGCTCAGGGACCTG
GAACTGGCTGTCGGAGGTTGTTGTGAGTAA

Upstream 100 bases:

>100_bases
GGTTTTCGGCAATTTCCGCCGCCCCTTGTTAACCAAATACCAAAGCAAAGTTGACAACTAGCAAATCCCTATGGTACTTC
AGGGCTCGGAGGTATCATCA

Downstream 100 bases:

>100_bases
CGCAATCTTCATCACGGGGACTGACACCGGAGTCGGGAAAACGATAGTAACGGCGACTCTGGCGTGCCTTTTGCGGAAAC
AAGGGATCAACGTGGGTGTC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNKSLETLAGRIIAGEQLSVEESIALSASSGTDAFALFLAASRVKEHFLGNGVDLCSIINAKSGRCPENCAFCAQSAHHT
TNAPIYPLVDEDKLVACAKEAEAAGSRCYGIITSGTTIKKGEELDRICRAVRRIRETTSIAPSCSLGIIDHETAVTLREA
GVETYHHNLETSRSFFPHICTTHDYEEDVETVRVAKQAGLTICCGGIFGLGETAAQRVELAMTLRELDVDSVPLNFLNPI
EGTRLAGSKLISPLECLKTIALFRLILPTKKIAVCGGREQNLRDLQSWIFFAGASGTMIGNYLTTTGRPAEQDWQMLRDL
ELAVGGCCE

Sequences:

>Translated_329_residues
MNKSLETLAGRIIAGEQLSVEESIALSASSGTDAFALFLAASRVKEHFLGNGVDLCSIINAKSGRCPENCAFCAQSAHHT
TNAPIYPLVDEDKLVACAKEAEAAGSRCYGIITSGTTIKKGEELDRICRAVRRIRETTSIAPSCSLGIIDHETAVTLREA
GVETYHHNLETSRSFFPHICTTHDYEEDVETVRVAKQAGLTICCGGIFGLGETAAQRVELAMTLRELDVDSVPLNFLNPI
EGTRLAGSKLISPLECLKTIALFRLILPTKKIAVCGGREQNLRDLQSWIFFAGASGTMIGNYLTTTGRPAEQDWQMLRDL
ELAVGGCCE
>Mature_329_residues
MNKSLETLAGRIIAGEQLSVEESIALSASSGTDAFALFLAASRVKEHFLGNGVDLCSIINAKSGRCPENCAFCAQSAHHT
TNAPIYPLVDEDKLVACAKEAEAAGSRCYGIITSGTTIKKGEELDRICRAVRRIRETTSIAPSCSLGIIDHETAVTLREA
GVETYHHNLETSRSFFPHICTTHDYEEDVETVRVAKQAGLTICCGGIFGLGETAAQRVELAMTLRELDVDSVPLNFLNPI
EGTRLAGSKLISPLECLKTIALFRLILPTKKIAVCGGREQNLRDLQSWIFFAGASGTMIGNYLTTTGRPAEQDWQMLRDL
ELAVGGCCE

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=291, Percent_Identity=32.9896907216495, Blast_Score=166, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321725, Length=264, Percent_Identity=30.3030303030303, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_GEOMG (Q39VB0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384539.1
- ProteinModelPortal:   Q39VB0
- SMR:   Q39VB0
- STRING:   Q39VB0
- GeneID:   3740563
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1582
- NMPDR:   fig|269799.3.peg.1421
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q39VB0
- ProtClustDB:   CLSK828421
- BioCyc:   GMET269799:GMET_1582-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35556; Mature: 35556

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSLETLAGRIIAGEQLSVEESIALSASSGTDAFALFLAASRVKEHFLGNGVDLCSIIN
CCCCHHHHHHHHCCCCCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHC
AKSGRCPENCAFCAQSAHHTTNAPIYPLVDEDKLVACAKEAEAAGSRCYGIITSGTTIKK
CCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
GEELDRICRAVRRIRETTSIAPSCSLGIIDHETAVTLREAGVETYHHNLETSRSFFPHIC
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTHDYEEDVETVRVAKQAGLTICCGGIFGLGETAAQRVELAMTLRELDVDSVPLNFLNPI
CCCCCHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
EGTRLAGSKLISPLECLKTIALFRLILPTKKIAVCGGREQNLRDLQSWIFFAGASGTMIG
CCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEC
NYLTTTGRPAEQDWQMLRDLELAVGGCCE
CEEECCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKSLETLAGRIIAGEQLSVEESIALSASSGTDAFALFLAASRVKEHFLGNGVDLCSIIN
CCCCHHHHHHHHCCCCCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHC
AKSGRCPENCAFCAQSAHHTTNAPIYPLVDEDKLVACAKEAEAAGSRCYGIITSGTTIKK
CCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
GEELDRICRAVRRIRETTSIAPSCSLGIIDHETAVTLREAGVETYHHNLETSRSFFPHIC
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTHDYEEDVETVRVAKQAGLTICCGGIFGLGETAAQRVELAMTLRELDVDSVPLNFLNPI
CCCCCHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
EGTRLAGSKLISPLECLKTIALFRLILPTKKIAVCGGREQNLRDLQSWIFFAGASGTMIG
CCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEC
NYLTTTGRPAEQDWQMLRDLELAVGGCCE
CEEECCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA