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The map label for this gene is bioK [H]

Identifier: 78222790

GI number: 78222790

Start: 1781457

End: 1782824

Strand: Reverse

Name: bioK [H]

Synonym: Gmet_1580

Alternate gene names: 78222790

Gene position: 1782824-1781457 (Counterclockwise)

Preceding gene: 78222791

Following gene: 78222789

Centisome position: 44.6

GC content: 57.09

Gene sequence:

>1368_bases
TTGAATACATCTGACACCAAAACACTTCGTGAGTACGACCGCGCCCATGTCTGGCACCCCTTCACCCAGATGAAGGAGTG
GGAGGAGAGTGACCCTGTCGTCATTGTCAGGGGCGAGGGTACCTGGATCGTTGATTCTGAGGGAAACCGCTACCTGGACG
GCGTCGCGGCCATCTGGACCAACGTCCACGGGCACTGCAAGCGTGAAATCAACGAAGCAATCAAGGCCCAGGTGGACACC
CTTGAGCACTCCACCCTCCTGGGACTCGCCAACGACAAAGCGGTGCTGCTTGCGCGGCGCCTTGTGGAGATTGCCCCGCC
AGGGCTCTGCAAGGTTTTTTACTCCGACAACGGTTCAACTGCCGTTGAGATCAGCGTCAAGATGGCCTTCCAGTACTGGC
AGCATCAAGGGAAACCGGAGAAAACAAAATTTATTTCGTTCAAAAATGCCTACCACGGCGACACCATCGGTGCCGTAAGC
GTCGGTGGTATCGACCTCTATCACGCTGTTTTCCGCCCCCTCCTCTTCCCAACAATCCAAGCCCCCGCCCCTTACTGTTA
CCGCTGCGAGTTCGGCGAGAGGGACCGCGCCAACTGCGGCATGCGCTGCCTCAAGGAACTGGAACGGCTCATGGAAATCC
ATGCCCATGAAGTTGCCGGCCTGGTGATCGAACCATTAGTCCAGGGCGCCGGTGGCATGATCGTACAGCCCGACGGTTTT
GTCCGGCGGGTCAGGGAACTCTGCGACCGCTACGACATCTTGATGATCGCCGATGAGGTGGCGGTAGGCTTCGGCCGCAC
CGGAGCCATGTTCGCCTGTGAGCGGGAGGGGATCACCCCCGACATCATGGCCCTTTCCAAGGGGATTACGGCAGGTTATC
TTCCCCTCGCCGCCACCATGACGACCCAGAAGATCTATGATGCCTTTATTGGCGAATACCGGGAGATGAAGACCTTCTTC
CACGGCCATACCTTCACCGGCAACCCCATTGCCTGCGCTGCGGCATTGGCGAGCCTCGACCTGTTCGAAAACGAACGACT
CCTCGATAGCCTCACCAACAAAATTGGCTACTTGGCAGAAAGACTTCAAGCATTAAATGAAGTCAGGCACGTGGGAGAAG
TACGCCAATGCGGCATGATCGCCGGCATCGAACTGGTGAAAGATGCAGAAACACGGGAACCATTCCCCTGGGAGGAGCGG
GTCGGCATTCAGGTATGTCTCGAAGCCCGTAACCGTGGTATTTTCCTCAGGCCCCTCGGCAACATCATAGTTGTATTCCC
CCCCCTCTCCATCAGCCTCGACGAACTCGCTTTCCTCATGGACGGTATTGAAGCTTCAATCAGGGCCATCACCGAATTGC
GAACCTGA

Upstream 100 bases:

>100_bases
CCCAAGGTGGAAGCGGCCGACCAGCGGACCATTGTCGAAACCCTGACCAACCACCTGTCTGGCCAAGGCACCACAAAAAT
TCTATTGCGGGAGATTGGCA

Downstream 100 bases:

>100_bases
CGTCTGCTTATTGTAAATAAAGAAGGGGGCGGCCACCATTTCGTGACCACCCCCTTTTTCTTCGATCCAAAAACCTGTTG
TCAGAATCTGCCGCGAAGAA

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MNTSDTKTLREYDRAHVWHPFTQMKEWEESDPVVIVRGEGTWIVDSEGNRYLDGVAAIWTNVHGHCKREINEAIKAQVDT
LEHSTLLGLANDKAVLLARRLVEIAPPGLCKVFYSDNGSTAVEISVKMAFQYWQHQGKPEKTKFISFKNAYHGDTIGAVS
VGGIDLYHAVFRPLLFPTIQAPAPYCYRCEFGERDRANCGMRCLKELERLMEIHAHEVAGLVIEPLVQGAGGMIVQPDGF
VRRVRELCDRYDILMIADEVAVGFGRTGAMFACEREGITPDIMALSKGITAGYLPLAATMTTQKIYDAFIGEYREMKTFF
HGHTFTGNPIACAAALASLDLFENERLLDSLTNKIGYLAERLQALNEVRHVGEVRQCGMIAGIELVKDAETREPFPWEER
VGIQVCLEARNRGIFLRPLGNIIVVFPPLSISLDELAFLMDGIEASIRAITELRT

Sequences:

>Translated_455_residues
MNTSDTKTLREYDRAHVWHPFTQMKEWEESDPVVIVRGEGTWIVDSEGNRYLDGVAAIWTNVHGHCKREINEAIKAQVDT
LEHSTLLGLANDKAVLLARRLVEIAPPGLCKVFYSDNGSTAVEISVKMAFQYWQHQGKPEKTKFISFKNAYHGDTIGAVS
VGGIDLYHAVFRPLLFPTIQAPAPYCYRCEFGERDRANCGMRCLKELERLMEIHAHEVAGLVIEPLVQGAGGMIVQPDGF
VRRVRELCDRYDILMIADEVAVGFGRTGAMFACEREGITPDIMALSKGITAGYLPLAATMTTQKIYDAFIGEYREMKTFF
HGHTFTGNPIACAAALASLDLFENERLLDSLTNKIGYLAERLQALNEVRHVGEVRQCGMIAGIELVKDAETREPFPWEER
VGIQVCLEARNRGIFLRPLGNIIVVFPPLSISLDELAFLMDGIEASIRAITELRT
>Mature_455_residues
MNTSDTKTLREYDRAHVWHPFTQMKEWEESDPVVIVRGEGTWIVDSEGNRYLDGVAAIWTNVHGHCKREINEAIKAQVDT
LEHSTLLGLANDKAVLLARRLVEIAPPGLCKVFYSDNGSTAVEISVKMAFQYWQHQGKPEKTKFISFKNAYHGDTIGAVS
VGGIDLYHAVFRPLLFPTIQAPAPYCYRCEFGERDRANCGMRCLKELERLMEIHAHEVAGLVIEPLVQGAGGMIVQPDGF
VRRVRELCDRYDILMIADEVAVGFGRTGAMFACEREGITPDIMALSKGITAGYLPLAATMTTQKIYDAFIGEYREMKTFF
HGHTFTGNPIACAAALASLDLFENERLLDSLTNKIGYLAERLQALNEVRHVGEVRQCGMIAGIELVKDAETREPFPWEER
VGIQVCLEARNRGIFLRPLGNIIVVFPPLSISLDELAFLMDGIEASIRAITELRT

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily [H]

Homologues:

Organism=Homo sapiens, GI37574042, Length=436, Percent_Identity=29.1284403669725, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI226442705, Length=436, Percent_Identity=28.6697247706422, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI24119277, Length=448, Percent_Identity=29.0178571428571, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI13994255, Length=445, Percent_Identity=27.8651685393258, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4557809, Length=454, Percent_Identity=30.6167400881057, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI226442709, Length=401, Percent_Identity=28.6783042394015, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI284507298, Length=247, Percent_Identity=33.6032388663968, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI188536080, Length=443, Percent_Identity=23.4762979683973, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI38679950, Length=443, Percent_Identity=23.4762979683973, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI38679946, Length=443, Percent_Identity=23.4762979683973, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786991, Length=450, Percent_Identity=37.3333333333333, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1788044, Length=417, Percent_Identity=29.2565947242206, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI145693181, Length=424, Percent_Identity=29.4811320754717, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789759, Length=360, Percent_Identity=31.9444444444444, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789016, Length=428, Percent_Identity=31.3084112149533, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787560, Length=405, Percent_Identity=31.1111111111111, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1786349, Length=359, Percent_Identity=25.9052924791086, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI32564660, Length=446, Percent_Identity=28.2511210762332, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI25144271, Length=433, Percent_Identity=26.7898383371824, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71992977, Length=446, Percent_Identity=27.5784753363229, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25144274, Length=242, Percent_Identity=28.9256198347107, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17541228, Length=383, Percent_Identity=23.2375979112272, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324386, Length=467, Percent_Identity=29.9785867237687, Blast_Score=209, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6323470, Length=450, Percent_Identity=26.4444444444444, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324432, Length=440, Percent_Identity=26.5909090909091, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6321456, Length=361, Percent_Identity=25.207756232687, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI28574759, Length=446, Percent_Identity=29.5964125560538, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI161085790, Length=447, Percent_Identity=29.082774049217, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21357415, Length=445, Percent_Identity=28.0898876404494, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21356575, Length=437, Percent_Identity=29.0617848970252, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI281366494, Length=282, Percent_Identity=27.3049645390071, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667139, Length=282, Percent_Identity=27.3049645390071, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667143, Length=282, Percent_Identity=27.3049645390071, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62

Molecular weight: Translated: 50868; Mature: 50868

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSDTKTLREYDRAHVWHPFTQMKEWEESDPVVIVRGEGTWIVDSEGNRYLDGVAAIWT
CCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHH
NVHGHCKREINEAIKAQVDTLEHSTLLGLANDKAVLLARRLVEIAPPGLCKVFYSDNGST
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCE
AVEISVKMAFQYWQHQGKPEKTKFISFKNAYHGDTIGAVSVGGIDLYHAVFRPLLFPTIQ
EEEEEHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCC
APAPYCYRCEFGERDRANCGMRCLKELERLMEIHAHEVAGLVIEPLVQGAGGMIVQPDGF
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHH
VRRVRELCDRYDILMIADEVAVGFGRTGAMFACEREGITPDIMALSKGITAGYLPLAATM
HHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHHH
TTQKIYDAFIGEYREMKTFFHGHTFTGNPIACAAALASLDLFENERLLDSLTNKIGYLAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RLQALNEVRHVGEVRQCGMIAGIELVKDAETREPFPWEERVGIQVCLEARNRGIFLRPLG
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHCCHHEEEEHHCCCEEEEECC
NIIVVFPPLSISLDELAFLMDGIEASIRAITELRT
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTSDTKTLREYDRAHVWHPFTQMKEWEESDPVVIVRGEGTWIVDSEGNRYLDGVAAIWT
CCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHH
NVHGHCKREINEAIKAQVDTLEHSTLLGLANDKAVLLARRLVEIAPPGLCKVFYSDNGST
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCE
AVEISVKMAFQYWQHQGKPEKTKFISFKNAYHGDTIGAVSVGGIDLYHAVFRPLLFPTIQ
EEEEEHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCC
APAPYCYRCEFGERDRANCGMRCLKELERLMEIHAHEVAGLVIEPLVQGAGGMIVQPDGF
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHH
VRRVRELCDRYDILMIADEVAVGFGRTGAMFACEREGITPDIMALSKGITAGYLPLAATM
HHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHHH
TTQKIYDAFIGEYREMKTFFHGHTFTGNPIACAAALASLDLFENERLLDSLTNKIGYLAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RLQALNEVRHVGEVRQCGMIAGIELVKDAETREPFPWEERVGIQVCLEARNRGIFLRPLG
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHCCHHEEEEHHCCCEEEEECC
NIIVVFPPLSISLDELAFLMDGIEASIRAITELRT
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]