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The map label for this gene is cca [H]

Identifier: 78222788

GI number: 78222788

Start: 1778079

End: 1780721

Strand: Direct

Name: cca [H]

Synonym: Gmet_1578

Alternate gene names: 78222788

Gene position: 1778079-1780721 (Clockwise)

Preceding gene: 78222787

Following gene: 78222793

Centisome position: 44.48

GC content: 56.64

Gene sequence:

>2643_bases
ATGGACGTTATCGCCACCCACGTGAACGCCGATTTTGACTGCCTTGGCGCCATGGTAGCAGCGAAGAAGCTCTATCCCGA
AGCCCTCATGGTCTTTCCCGGATCCCAGGAAAAGAGCATGAGGGACTTCTTTCTCAAGACCACCGGTTATATGCCGGCCT
TGACCCGCCTGAAGGACATTGATCCTGCCAAGATATCACGGCTTATCCTCGTTGATTGCCGTCACACATCGCGCATCGGC
CGCCTGGCCGAGGTCGCGAAACGGCCTGACGTCGAAGTTCATATCTACGACCATCATCCAGACTCCGCGGGAGACATTAC
CAAGAGCGGCGGCGAGATCCGTGAGTGCGGCTCATCCACGACTATTCTGACCAAGATACTCATGAAAAAGGGGATCGAGG
TGACGCCGGTTGAAGCGACCCTCATGATGCTCGGCATTTACGAGGATACCGGAAACCTTACCTTTCCATCCACGACGGTT
GATGACTATGGCGCAGCTTCGTGGCTTCTTGCCCATGGAGCAAACCTGAATGTTGTTGCCGAATTCGTTTCCCAGGAACT
AACCCCGGAGCAGGTGTCGCTATTGAACGACCTTCTCCATTCGCTGCAGACGGCGAGCGTTAATGGTGTCGATATTTCGC
TCGCTCACGCGTCCATTGACCATTATGTGGGAGATGTTGCCGTGCTGGCCCACATGATGCGCGATATGGAAAACCTTGAA
GCGCTCTTCATCGTGGTCGGCATGGGGGAGCGGGTTTACATCGTAGCTCGAAGCCGCATCCCTGAAGTGGACGTGGGGGC
GATCCTGCGCGAGTTGGGCGGTGGAGGCCATGCAACCGCCGCCTCCGCAACGGTTCGCGATCATACTGTCATCCAGGTCT
TGGCACGACTCAAACAACTTTTCCCCGAATGGATCAACCCTAAGCGTACGGCCGGCGACCTCATGTCGTCGCCGGTGAAG
ACGCTGCCGGTGGAGACCACCATTGCCGAAGCCCGCGAACTCCTGACCCGCTACAATGTGAACTCCATGCCGGTTATCGA
GGGAGGGGGGATGGTCGGGATCATCTCTCTCCGGATCGTGGAGAAGGCCCTCTATCACGGGCTCGGCTCCCTGCCGGTCA
GTGAATATATGCATACGGAGTTTATGCGTGCCGAACCCGACACCCCCATCGCCGTCATCCAGAACTACATCGTCGGGCAG
CATCGGCGGCTGGTCCCTGTCTTTTCCGGCGAGGATCTTGTGGGGGTCATAACGCGGACCGATCTCCTCCGCTATATGTA
CTCCGGCATGCAGCACAGCGCCGAGGCGGTCTACGATCTCGGCAGGGAAAACCCGCCGGTACGCCGTCGCGAGGTGGTCC
ACCTCATGAACAAGAACCTTCCGCGCAGGGTGGTGGATACGCTTCGCGAGCTGGGGAAGGTAGGGGATGGGCTTGAACTG
CCTGTATTCGCCATCGGCGGATTTGCCAGGGATTTGCTGCTGGGCGGGAAGAACGACGATATCGACATTACGGTGGAGGG
GGATGGCATCCTCTTTGCCGAAACCTTTGCGGCGACCCATGAGTGCCGCGTCAAGAGCCACGCCAAGTTCGGTACCGCTG
TCATTGTGCTTCCCGATGGTTTCAAGATCGATGTGGCAAGTACCCGCCTTGAGTTCTACGAAACTCCCGGCGCCCTTCCA
ACCGTGGAGCGTTCCTCCCTTAAGATGGATCTGTACCGGCGTGACTTCACCATCAACACCCTTGCGATCCAGCTCAACCC
AGCCAACTTCGGGCTTCTCATCGATTATTTCGGTGCCTACCGCGATTTGCGGGACAAGGTCATCCGGGTCCTCCACAATC
TCTCGTTCGTGGAGGATCCGACGCGGGTCTTCCGGGCTGTCCGCTTTGAGCAACGACTCGGGTTCCAGATTGCACGGCAT
ACGGATAATCTCATCAGGAATGCCGTAAAGATGGGCTTTCTCGACAAACTCGGCGGCAGGCGCCTTCTGAACGAGTTGGT
CATCATCCTCCGGGAGCGGGAGCCGGTCCGAGCAATCCTGCGTATGGAAAGTCTGGGGTTGTTGGGTTTCATTCATCCTG
ACCTGACGCTTGAAACTGAAAATCTGAGGGTACTGGACGAGGTCAAGAAGGTTGTTACCTGGTTCGAACTGCTCTACCAG
AGAGACCAGGTGGAGACATGGGTGGTCTATTTTCTTGCCCTCACCTCACTCCTTGCCGATGCTGCATTCTTTGAGATGTG
CACACGGCTATCCGTTTCGGAACATTACCGGGAGAAGCTCATTGATATGAGGGTGCATGGCGAGCAGATGCTGGCTGTGA
TGGAGCGAAAAGTTGCCGGGAAGAAGCCGGTCCTGCGGAGCAACATCTACTTCTGGCTTCGTGGTCTCTCCCCTGAGGTG
CTTCTGTACATAATGGCCAAGACTTGTCGCGATGAGGTGCGGAAGTTCGTCTCCCTCTACGTCACACAACTGCGTGAAGT
TACCTGTATGCTTACCGGAGATGATCTCAAGGCTATCGGACTTCCACCGGGACCTCGTTACCGAGAGATCCTCGATGCGG
TACTTTCGGCGCGACTGAATGGAGAAGTGTCAACGAGGGAGGAGGAATCCCGGTTCGTCAAACAGATGCTCAACTTTGTG
TAG

Upstream 100 bases:

>100_bases
GGAGAAAATCCCGTCTATCAACCGTTCCCCCTTAAGCCGATGGCGGCGTCCCGCTGATCTTTTCCTTTACTTTTGCCGCT
GATACGGCACACTCTCTTCC

Downstream 100 bases:

>100_bases
CGATTACGCCACGGTCCGTCAAACCTTTAGCCTACTGACTAAACTGACCATTTTGAGCACGTTCGGAAATTTTTGACAGG
GCCGCCGGCCTCTTGCTGTA

Product: PolyA polymerase family protein

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 880; Mature: 880

Protein sequence:

>880_residues
MDVIATHVNADFDCLGAMVAAKKLYPEALMVFPGSQEKSMRDFFLKTTGYMPALTRLKDIDPAKISRLILVDCRHTSRIG
RLAEVAKRPDVEVHIYDHHPDSAGDITKSGGEIRECGSSTTILTKILMKKGIEVTPVEATLMMLGIYEDTGNLTFPSTTV
DDYGAASWLLAHGANLNVVAEFVSQELTPEQVSLLNDLLHSLQTASVNGVDISLAHASIDHYVGDVAVLAHMMRDMENLE
ALFIVVGMGERVYIVARSRIPEVDVGAILRELGGGGHATAASATVRDHTVIQVLARLKQLFPEWINPKRTAGDLMSSPVK
TLPVETTIAEARELLTRYNVNSMPVIEGGGMVGIISLRIVEKALYHGLGSLPVSEYMHTEFMRAEPDTPIAVIQNYIVGQ
HRRLVPVFSGEDLVGVITRTDLLRYMYSGMQHSAEAVYDLGRENPPVRRREVVHLMNKNLPRRVVDTLRELGKVGDGLEL
PVFAIGGFARDLLLGGKNDDIDITVEGDGILFAETFAATHECRVKSHAKFGTAVIVLPDGFKIDVASTRLEFYETPGALP
TVERSSLKMDLYRRDFTINTLAIQLNPANFGLLIDYFGAYRDLRDKVIRVLHNLSFVEDPTRVFRAVRFEQRLGFQIARH
TDNLIRNAVKMGFLDKLGGRRLLNELVIILREREPVRAILRMESLGLLGFIHPDLTLETENLRVLDEVKKVVTWFELLYQ
RDQVETWVVYFLALTSLLADAAFFEMCTRLSVSEHYREKLIDMRVHGEQMLAVMERKVAGKKPVLRSNIYFWLRGLSPEV
LLYIMAKTCRDEVRKFVSLYVTQLREVTCMLTGDDLKAIGLPPGPRYREILDAVLSARLNGEVSTREEESRFVKQMLNFV

Sequences:

>Translated_880_residues
MDVIATHVNADFDCLGAMVAAKKLYPEALMVFPGSQEKSMRDFFLKTTGYMPALTRLKDIDPAKISRLILVDCRHTSRIG
RLAEVAKRPDVEVHIYDHHPDSAGDITKSGGEIRECGSSTTILTKILMKKGIEVTPVEATLMMLGIYEDTGNLTFPSTTV
DDYGAASWLLAHGANLNVVAEFVSQELTPEQVSLLNDLLHSLQTASVNGVDISLAHASIDHYVGDVAVLAHMMRDMENLE
ALFIVVGMGERVYIVARSRIPEVDVGAILRELGGGGHATAASATVRDHTVIQVLARLKQLFPEWINPKRTAGDLMSSPVK
TLPVETTIAEARELLTRYNVNSMPVIEGGGMVGIISLRIVEKALYHGLGSLPVSEYMHTEFMRAEPDTPIAVIQNYIVGQ
HRRLVPVFSGEDLVGVITRTDLLRYMYSGMQHSAEAVYDLGRENPPVRRREVVHLMNKNLPRRVVDTLRELGKVGDGLEL
PVFAIGGFARDLLLGGKNDDIDITVEGDGILFAETFAATHECRVKSHAKFGTAVIVLPDGFKIDVASTRLEFYETPGALP
TVERSSLKMDLYRRDFTINTLAIQLNPANFGLLIDYFGAYRDLRDKVIRVLHNLSFVEDPTRVFRAVRFEQRLGFQIARH
TDNLIRNAVKMGFLDKLGGRRLLNELVIILREREPVRAILRMESLGLLGFIHPDLTLETENLRVLDEVKKVVTWFELLYQ
RDQVETWVVYFLALTSLLADAAFFEMCTRLSVSEHYREKLIDMRVHGEQMLAVMERKVAGKKPVLRSNIYFWLRGLSPEV
LLYIMAKTCRDEVRKFVSLYVTQLREVTCMLTGDDLKAIGLPPGPRYREILDAVLSARLNGEVSTREEESRFVKQMLNFV
>Mature_880_residues
MDVIATHVNADFDCLGAMVAAKKLYPEALMVFPGSQEKSMRDFFLKTTGYMPALTRLKDIDPAKISRLILVDCRHTSRIG
RLAEVAKRPDVEVHIYDHHPDSAGDITKSGGEIRECGSSTTILTKILMKKGIEVTPVEATLMMLGIYEDTGNLTFPSTTV
DDYGAASWLLAHGANLNVVAEFVSQELTPEQVSLLNDLLHSLQTASVNGVDISLAHASIDHYVGDVAVLAHMMRDMENLE
ALFIVVGMGERVYIVARSRIPEVDVGAILRELGGGGHATAASATVRDHTVIQVLARLKQLFPEWINPKRTAGDLMSSPVK
TLPVETTIAEARELLTRYNVNSMPVIEGGGMVGIISLRIVEKALYHGLGSLPVSEYMHTEFMRAEPDTPIAVIQNYIVGQ
HRRLVPVFSGEDLVGVITRTDLLRYMYSGMQHSAEAVYDLGRENPPVRRREVVHLMNKNLPRRVVDTLRELGKVGDGLEL
PVFAIGGFARDLLLGGKNDDIDITVEGDGILFAETFAATHECRVKSHAKFGTAVIVLPDGFKIDVASTRLEFYETPGALP
TVERSSLKMDLYRRDFTINTLAIQLNPANFGLLIDYFGAYRDLRDKVIRVLHNLSFVEDPTRVFRAVRFEQRLGFQIARH
TDNLIRNAVKMGFLDKLGGRRLLNELVIILREREPVRAILRMESLGLLGFIHPDLTLETENLRVLDEVKKVVTWFELLYQ
RDQVETWVVYFLALTSLLADAAFFEMCTRLSVSEHYREKLIDMRVHGEQMLAVMERKVAGKKPVLRSNIYFWLRGLSPEV
LLYIMAKTCRDEVRKFVSLYVTQLREVTCMLTGDDLKAIGLPPGPRYREILDAVLSARLNGEVSTREEESRFVKQMLNFV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=231, Percent_Identity=29.8701298701299, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 98647; Mature: 98647

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVIATHVNADFDCLGAMVAAKKLYPEALMVFPGSQEKSMRDFFLKTTGYMPALTRLKDI
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHCCC
DPAKISRLILVDCRHTSRIGRLAEVAKRPDVEVHIYDHHPDSAGDITKSGGEIRECGSST
CHHHHCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCH
TILTKILMKKGIEVTPVEATLMMLGIYEDTGNLTFPSTTVDDYGAASWLLAHGANLNVVA
HHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCEECCCCCCCCCCCHHHHHHCCCCHHHHH
EFVSQELTPEQVSLLNDLLHSLQTASVNGVDISLAHASIDHYVGDVAVLAHMMRDMENLE
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCE
ALFIVVGMGERVYIVARSRIPEVDVGAILRELGGGGHATAASATVRDHTVIQVLARLKQL
EEEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FPEWINPKRTAGDLMSSPVKTLPVETTIAEARELLTRYNVNSMPVIEGGGMVGIISLRIV
HHHHCCCCHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCEEEHHHHHHH
EKALYHGLGSLPVSEYMHTEFMRAEPDTPIAVIQNYIVGQHRRLVPVFSGEDLVGVITRT
HHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHH
DLLRYMYSGMQHSAEAVYDLGRENPPVRRREVVHLMNKNLPRRVVDTLRELGKVGDGLEL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
PVFAIGGFARDLLLGGKNDDIDITVEGDGILFAETFAATHECRVKSHAKFGTAVIVLPDG
CHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCCCCEEEEEECCC
FKIDVASTRLEFYETPGALPTVERSSLKMDLYRRDFTINTLAIQLNPANFGLLIDYFGAY
CEEEEEHHHHHHEECCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHH
RDLRDKVIRVLHNLSFVEDPTRVFRAVRFEQRLGFQIARHTDNLIRNAVKMGFLDKLGGR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCHH
RLLNELVIILREREPVRAILRMESLGLLGFIHPDLTLETENLRVLDEVKKVVTWFELLYQ
HHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHH
RDQVETWVVYFLALTSLLADAAFFEMCTRLSVSEHYREKLIDMRVHGEQMLAVMERKVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKPVLRSNIYFWLRGLSPEVLLYIMAKTCRDEVRKFVSLYVTQLREVTCMLTGDDLKAIG
CCCCHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEC
LPPGPRYREILDAVLSARLNGEVSTREEESRFVKQMLNFV
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDVIATHVNADFDCLGAMVAAKKLYPEALMVFPGSQEKSMRDFFLKTTGYMPALTRLKDI
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHCCC
DPAKISRLILVDCRHTSRIGRLAEVAKRPDVEVHIYDHHPDSAGDITKSGGEIRECGSST
CHHHHCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCH
TILTKILMKKGIEVTPVEATLMMLGIYEDTGNLTFPSTTVDDYGAASWLLAHGANLNVVA
HHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCEECCCCCCCCCCCHHHHHHCCCCHHHHH
EFVSQELTPEQVSLLNDLLHSLQTASVNGVDISLAHASIDHYVGDVAVLAHMMRDMENLE
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCE
ALFIVVGMGERVYIVARSRIPEVDVGAILRELGGGGHATAASATVRDHTVIQVLARLKQL
EEEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FPEWINPKRTAGDLMSSPVKTLPVETTIAEARELLTRYNVNSMPVIEGGGMVGIISLRIV
HHHHCCCCHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCEEEHHHHHHH
EKALYHGLGSLPVSEYMHTEFMRAEPDTPIAVIQNYIVGQHRRLVPVFSGEDLVGVITRT
HHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHH
DLLRYMYSGMQHSAEAVYDLGRENPPVRRREVVHLMNKNLPRRVVDTLRELGKVGDGLEL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
PVFAIGGFARDLLLGGKNDDIDITVEGDGILFAETFAATHECRVKSHAKFGTAVIVLPDG
CHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCCCCEEEEEECCC
FKIDVASTRLEFYETPGALPTVERSSLKMDLYRRDFTINTLAIQLNPANFGLLIDYFGAY
CEEEEEHHHHHHEECCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHH
RDLRDKVIRVLHNLSFVEDPTRVFRAVRFEQRLGFQIARHTDNLIRNAVKMGFLDKLGGR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCHH
RLLNELVIILREREPVRAILRMESLGLLGFIHPDLTLETENLRVLDEVKKVVTWFELLYQ
HHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHH
RDQVETWVVYFLALTSLLADAAFFEMCTRLSVSEHYREKLIDMRVHGEQMLAVMERKVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKPVLRSNIYFWLRGLSPEVLLYIMAKTCRDEVRKFVSLYVTQLREVTCMLTGDDLKAIG
CCCCHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEC
LPPGPRYREILDAVLSARLNGEVSTREEESRFVKQMLNFV
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA