BacMap

Definition	Geobacter metallireducens GS-15 chromosome, complete genome.
Accession	NC_007517
Length	3,997,420

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The map label for this gene is paaF [C]

Identifier: 78222782

GI number: 78222782

Start: 1773608

End: 1774387

Strand: Direct

Name: paaF [C]

Synonym: Gmet_1572

Alternate gene names: 78222782

Gene position: 1773608-1774387 (Clockwise)

Preceding gene: 78222781

Following gene: 78222783

Centisome position: 44.37

GC content: 57.31

Gene sequence:

>780_bases
TTGAGCGCAAAACAGATTGCCGTCAGGCATGATGATGCCATTGCCGTTGTTTCTTTGGCCAGACCTGAGTCACGTAACGT
CTTGAGTCGAGATTTGGTTTTGGGTTTGCTCTCTACATTCACCTCGCTTAAGGACGATGGGCGGGTGAAGGGGATTGTGG
TTACTGGTGAGGGAAAGAGTTTCTGTGCCGGCGCTGATATCTCCGAGATGGCCCGCATGAGTCCCGCCGAGGCGTCGTCA
TTCGCCGAGCTGGGGCAACGGCTCATGTTTGCCGTCGAGAGGGTCGGAAAACCGGTGGTGGCTGCTGTGAACGGTCATGC
TTTTGGTGGCGGGCTTGAACTGGCGCTGGCTTGTGATTTCATTGTTGCTGCAGAGTCAGCAGTCTTCGCCGCTCCCGAGG
TTCTTCTCGGCGTCATGCCCGGCTTCGGCGGCACGCAGCGTCTGCCGCGGTTGATCGGCAAGTCACGGGCAAAGGAGATG
ATATTCACCGGGGAGCGGATAAATGCGGCGAAGGCCCACTCCATTGGGCTTGTCAACCGTGTTGTCTCTGATGAGAGGCT
TCTCGCTGAGACAGTCTCGTTGGTCAAGAATATCTGCAACCGTGGGCTTCTTTCCCTCCGCGTGGCGAAGGAGGTCATCG
ACGCCGGGGCGGGTATCGATCTTGCCACAGCCTGCTTGATGGAACGCGATGCCTTTGCGCTTTGCTTTTCCACTGACGAC
CAAAAAGAAGGGATGCGCGCCTTCATGGAAAAGCGGGAACCCCGCTTCACCGGACGGTAA

Upstream 100 bases:

>100_bases
AATCGTTATACTTTCCGTTGAATACACCTTGACAGCCAATCGGCGCTGTTGATATAGTCCGTCACTGCGGTTTTTATCGG
TACTAAAACAGGAGATGGTT

Downstream 100 bases:

>100_bases
CTGGGAAGTGACCATGAAACTGGAGCAAGGGTGTATTCAAATTTACACGGGTAATGGCAAGGGGAAGACCACTGCTGCCC
TCGGACTTGCTTTTCGTGCC

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSAKQIAVRHDDAIAVVSLARPESRNVLSRDLVLGLLSTFTSLKDDGRVKGIVVTGEGKSFCAGADISEMARMSPAEASS
FAELGQRLMFAVERVGKPVVAAVNGHAFGGGLELALACDFIVAAESAVFAAPEVLLGVMPGFGGTQRLPRLIGKSRAKEM
IFTGERINAAKAHSIGLVNRVVSDERLLAETVSLVKNICNRGLLSLRVAKEVIDAGAGIDLATACLMERDAFALCFSTDD
QKEGMRAFMEKREPRFTGR

Sequences:

>Translated_259_residues
MSAKQIAVRHDDAIAVVSLARPESRNVLSRDLVLGLLSTFTSLKDDGRVKGIVVTGEGKSFCAGADISEMARMSPAEASS
FAELGQRLMFAVERVGKPVVAAVNGHAFGGGLELALACDFIVAAESAVFAAPEVLLGVMPGFGGTQRLPRLIGKSRAKEM
IFTGERINAAKAHSIGLVNRVVSDERLLAETVSLVKNICNRGLLSLRVAKEVIDAGAGIDLATACLMERDAFALCFSTDD
QKEGMRAFMEKREPRFTGR
>Mature_258_residues
SAKQIAVRHDDAIAVVSLARPESRNVLSRDLVLGLLSTFTSLKDDGRVKGIVVTGEGKSFCAGADISEMARMSPAEASSF
AELGQRLMFAVERVGKPVVAAVNGHAFGGGLELALACDFIVAAESAVFAAPEVLLGVMPGFGGTQRLPRLIGKSRAKEMI
FTGERINAAKAHSIGLVNRVVSDERLLAETVSLVKNICNRGLLSLRVAKEVIDAGAGIDLATACLMERDAFALCFSTDDQ
KEGMRAFMEKREPRFTGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=41.9354838709677, Blast_Score=191, Evalue=4e-49,
Organism=Homo sapiens, GI4502327, Length=263, Percent_Identity=37.6425855513308, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=33.4645669291339, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI20127408, Length=174, Percent_Identity=36.2068965517241, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=30, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI68989263, Length=234, Percent_Identity=32.4786324786325, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI70995211, Length=184, Percent_Identity=34.2391304347826, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=28.2051282051282, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI157694516, Length=234, Percent_Identity=28.2051282051282, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI37594471, Length=250, Percent_Identity=28.8, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI37594469, Length=250, Percent_Identity=28.8, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI45643119, Length=213, Percent_Identity=26.2910798122066, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260275230, Length=213, Percent_Identity=26.2910798122066, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260274832, Length=213, Percent_Identity=26.2910798122066, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=35.5555555555556, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4757968, Length=262, Percent_Identity=22.5190839694656, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI49355787, Length=262, Percent_Identity=22.5190839694656, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI221136756, Length=259, Percent_Identity=23.5521235521236, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI221136753, Length=259, Percent_Identity=23.5521235521236, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI25453481, Length=261, Percent_Identity=22.2222222222222, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI51538573, Length=261, Percent_Identity=22.2222222222222, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI221307494, Length=255, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI157694520, Length=211, Percent_Identity=28.436018957346, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI116235487, Length=158, Percent_Identity=30.379746835443, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI62530384, Length=245, Percent_Identity=24.4897959183673, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4757966, Length=229, Percent_Identity=22.707423580786, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI51538581, Length=229, Percent_Identity=22.707423580786, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI295842266, Length=243, Percent_Identity=23.8683127572016, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=37.2549019607843, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=33.969465648855, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=41.2935323383085, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=32, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87082183, Length=271, Percent_Identity=28.4132841328413, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=30.2788844621514, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1790281, Length=201, Percent_Identity=35.3233830845771, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=39.2, Blast_Score=188, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=39.8373983739837, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=36.4444444444444, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17558304, Length=175, Percent_Identity=41.7142857142857, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=261, Percent_Identity=30.2681992337165, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25144276, Length=175, Percent_Identity=41.7142857142857, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17508953, Length=175, Percent_Identity=41.7142857142857, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17508951, Length=175, Percent_Identity=41.7142857142857, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=27.7056277056277, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=29.3577981651376, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=199, Percent_Identity=30.1507537688442, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=27.5720164609054, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=23.3201581027668, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=200, Percent_Identity=27, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=219, Percent_Identity=28.7671232876712, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=219, Percent_Identity=28.7671232876712, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=187, Percent_Identity=27.807486631016, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=41.3934426229508, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=41.3934426229508, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=39.2, Blast_Score=174, Evalue=7e-44,
Organism=Drosophila melanogaster, GI21357171, Length=183, Percent_Identity=37.7049180327869, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920382, Length=209, Percent_Identity=32.0574162679426, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=25.0996015936255, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=34.1463414634146, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921000, Length=134, Percent_Identity=40.2985074626866, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583077, Length=134, Percent_Identity=40.2985074626866, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583079, Length=134, Percent_Identity=40.2985074626866, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28571730, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571729, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27568; Mature: 27437

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKQIAVRHDDAIAVVSLARPESRNVLSRDLVLGLLSTFTSLKDDGRVKGIVVTGEGKS
CCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FCAGADISEMARMSPAEASSFAELGQRLMFAVERVGKPVVAAVNGHAFGGGLELALACDF
EECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
IVAAESAVFAAPEVLLGVMPGFGGTQRLPRLIGKSRAKEMIFTGERINAAKAHSIGLVNR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VVSDERLLAETVSLVKNICNRGLLSLRVAKEVIDAGAGIDLATACLMERDAFALCFSTDD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCC
QKEGMRAFMEKREPRFTGR
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAKQIAVRHDDAIAVVSLARPESRNVLSRDLVLGLLSTFTSLKDDGRVKGIVVTGEGKS
CCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FCAGADISEMARMSPAEASSFAELGQRLMFAVERVGKPVVAAVNGHAFGGGLELALACDF
EECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
IVAAESAVFAAPEVLLGVMPGFGGTQRLPRLIGKSRAKEMIFTGERINAAKAHSIGLVNR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VVSDERLLAETVSLVKNICNRGLLSLRVAKEVIDAGAGIDLATACLMERDAFALCFSTDD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCC
QKEGMRAFMEKREPRFTGR
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA