Click here to switch to the map view.

The map label for this gene is gdh [H]

Identifier: 78222401

GI number: 78222401

Start: 1317800

End: 1319152

Strand: Reverse

Name: gdh [H]

Synonym: Gmet_1186

Alternate gene names: 78222401

Gene position: 1319152-1317800 (Counterclockwise)

Preceding gene: 78222405

Following gene: 78222399

Centisome position: 33.0

GC content: 57.5

Gene sequence:

>1353_bases
ATGTCACCACAACTTATCGACGAAAGACTCGCAGGCATCTATCAGGATGTGCTCAAGCGCAACCCGGGCGAGGTGGAATT
CCACCAGGCCGTGGCCGAGGTCCTGGAATCCCTCGGACCGGTCGTGACCAAGCACCCGGAATACCTGGAGCGCAAAATCA
TTGAGCGGATCGCTGAACCGGAGCGTCAGATTATCTTCCGGGTGCCCTGGCAGGACGACAAGGGGCAGGTCCACATCAAC
CGAGGCTTCCGCGTTGAATTCAACTCCGCTCTCGGCCCCTATAAGGGAGGCCTCCGCTTCCACCCCTCCGTCTATCTCGG
CATTATCAAGTTCCTCGGCTTCGAGCAGATCTTCAAGAACTCCCTCACCGGCATGCCGATCGGTGGCGGCAAAGGTGGTT
CCGACTTCGATCCGAAAGGGAAGACCGATGACGAAGTAATGCGTTTCTGCCAGAGCTTCATGACTGAACTCTATCGCCAC
ATTGGCGAACACACCGATGTTCCGGCAGGAGACATCGGGGTTGGCGGCCGCGAAATCGGCTACATGTTCGGCCAGTACAA
GCGGATCACTAACCGCTGGGAAGCCGGCGTCCTCACCGGCAAGGGACTCAAGTTCGGCGGATCCCTGGTTCGTCCGGAAG
CAACAGGCTACGGCGCCACATACTTCATCAACGAAGCCCTCAAGGTACGCAAAGATTCCTTCGACGGCAAGACCTGCCTG
GTATCAGGTTCAGGTAACGTGGCCATCTACACCATCGAGAAGATCCACCAACTGGGTGGCAAGTGCATTGCCTGCTCCGA
CAGTAACGGCGTCATCATCCACGAGAAAGGGCTTGATGTGGAACTGATCAAGCAGCTGAAGGAAGTGGAGCGTCGCCGGA
TCGCCGATTACGCCACCTACCACAAGGATGCCAAGTACATCGCCAACGGCAACATCTGGGATATCCCCTGCCAGGTAGCC
ATGCCATCAGCAACCCAGAACGAGATCAACGGCAAGGATGCCAAGACCCTGGTGAAGAACGGTTGTATTGCCGTGGGCGA
AGGGGCAAACATGCCCACCACGCCGGAAGGGATCAAGGTGTTCATCGAGGCCCAAATTGCCTACGGTCCAGGCAAGGCGG
CCAATGCCGGCGGGGTTGCCACCTCGGCCCTCGAAATGCAACAGAACGCCCAGCGCGATTCCTGGTCCTTCGAAGAGACC
GAGAAGAAGCTGGAGAAGATCATGGTCGGCATTCACAAGCTCTGCTACGAAACTGCCGAAGAGTATGGCGACAAGGGGAA
CTATGTCACCGGCGCCAACATCGCCGGTTTCATCAAGGTAGCTGACGCCATGGTCGCTCACGGTCTGGTATAA

Upstream 100 bases:

>100_bases
TGCACCCTCTGCATATATAAGTGGCGCCAGCCAAGAAGGCAATTAACCCGATTCTGAGCACATGTTGAACAGGCTCACAA
TATCATCACAAGGAGGAAGC

Downstream 100 bases:

>100_bases
GCAGCACTCGTTCATTCATAACCCCAAAAAGGCCCGGCATTGCCGGGCTTTTTTCAGGTTCATCCGGGAATTCCGGGGAA
AGCGGCTCTGATCTTGAGAA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSPQLIDERLAGIYQDVLKRNPGEVEFHQAVAEVLESLGPVVTKHPEYLERKIIERIAEPERQIIFRVPWQDDKGQVHIN
RGFRVEFNSALGPYKGGLRFHPSVYLGIIKFLGFEQIFKNSLTGMPIGGGKGGSDFDPKGKTDDEVMRFCQSFMTELYRH
IGEHTDVPAGDIGVGGREIGYMFGQYKRITNRWEAGVLTGKGLKFGGSLVRPEATGYGATYFINEALKVRKDSFDGKTCL
VSGSGNVAIYTIEKIHQLGGKCIACSDSNGVIIHEKGLDVELIKQLKEVERRRIADYATYHKDAKYIANGNIWDIPCQVA
MPSATQNEINGKDAKTLVKNGCIAVGEGANMPTTPEGIKVFIEAQIAYGPGKAANAGGVATSALEMQQNAQRDSWSFEET
EKKLEKIMVGIHKLCYETAEEYGDKGNYVTGANIAGFIKVADAMVAHGLV

Sequences:

>Translated_450_residues
MSPQLIDERLAGIYQDVLKRNPGEVEFHQAVAEVLESLGPVVTKHPEYLERKIIERIAEPERQIIFRVPWQDDKGQVHIN
RGFRVEFNSALGPYKGGLRFHPSVYLGIIKFLGFEQIFKNSLTGMPIGGGKGGSDFDPKGKTDDEVMRFCQSFMTELYRH
IGEHTDVPAGDIGVGGREIGYMFGQYKRITNRWEAGVLTGKGLKFGGSLVRPEATGYGATYFINEALKVRKDSFDGKTCL
VSGSGNVAIYTIEKIHQLGGKCIACSDSNGVIIHEKGLDVELIKQLKEVERRRIADYATYHKDAKYIANGNIWDIPCQVA
MPSATQNEINGKDAKTLVKNGCIAVGEGANMPTTPEGIKVFIEAQIAYGPGKAANAGGVATSALEMQQNAQRDSWSFEET
EKKLEKIMVGIHKLCYETAEEYGDKGNYVTGANIAGFIKVADAMVAHGLV
>Mature_449_residues
SPQLIDERLAGIYQDVLKRNPGEVEFHQAVAEVLESLGPVVTKHPEYLERKIIERIAEPERQIIFRVPWQDDKGQVHINR
GFRVEFNSALGPYKGGLRFHPSVYLGIIKFLGFEQIFKNSLTGMPIGGGKGGSDFDPKGKTDDEVMRFCQSFMTELYRHI
GEHTDVPAGDIGVGGREIGYMFGQYKRITNRWEAGVLTGKGLKFGGSLVRPEATGYGATYFINEALKVRKDSFDGKTCLV
SGSGNVAIYTIEKIHQLGGKCIACSDSNGVIIHEKGLDVELIKQLKEVERRRIADYATYHKDAKYIANGNIWDIPCQVAM
PSATQNEINGKDAKTLVKNGCIAVGEGANMPTTPEGIKVFIEAQIAYGPGKAANAGGVATSALEMQQNAQRDSWSFEETE
KKLEKIMVGIHKLCYETAEEYGDKGNYVTGANIAGFIKVADAMVAHGLV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=356, Percent_Identity=31.7415730337079, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI4885281, Length=356, Percent_Identity=31.7415730337079, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1788059, Length=443, Percent_Identity=54.4018058690745, Blast_Score=493, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17544676, Length=415, Percent_Identity=24.8192771084337, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324951, Length=454, Percent_Identity=49.5594713656388, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319256, Length=456, Percent_Identity=49.3421052631579, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553475, Length=390, Percent_Identity=28.974358974359, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45549226, Length=356, Percent_Identity=29.7752808988764, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24649283, Length=419, Percent_Identity=27.4463007159904, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24649460, Length=291, Percent_Identity=30.2405498281787, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 49432; Mature: 49301

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPQLIDERLAGIYQDVLKRNPGEVEFHQAVAEVLESLGPVVTKHPEYLERKIIERIAEP
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHCCC
ERQIIFRVPWQDDKGQVHINRGFRVEFNSALGPYKGGLRFHPSVYLGIIKFLGFEQIFKN
CEEEEEECCCCCCCCEEEECCCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHC
SLTGMPIGGGKGGSDFDPKGKTDDEVMRFCQSFMTELYRHIGEHTDVPAGDIGVGGREIG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
YMFGQYKRITNRWEAGVLTGKGLKFGGSLVRPEATGYGATYFINEALKVRKDSFDGKTCL
HHHHHHHHHHHHHHCCEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
VSGSGNVAIYTIEKIHQLGGKCIACSDSNGVIIHEKGLDVELIKQLKEVERRRIADYATY
EECCCCEEEEEHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
HKDAKYIANGNIWDIPCQVAMPSATQNEINGKDAKTLVKNGCIAVGEGANMPTTPEGIKV
HCCCCEEECCCEEECCCCEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCCEEE
FIEAQIAYGPGKAANAGGVATSALEMQQNAQRDSWSFEETEKKLEKIMVGIHKLCYETAE
EEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EYGDKGNYVTGANIAGFIKVADAMVAHGLV
HHCCCCCEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPQLIDERLAGIYQDVLKRNPGEVEFHQAVAEVLESLGPVVTKHPEYLERKIIERIAEP
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHCCC
ERQIIFRVPWQDDKGQVHINRGFRVEFNSALGPYKGGLRFHPSVYLGIIKFLGFEQIFKN
CEEEEEECCCCCCCCEEEECCCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHC
SLTGMPIGGGKGGSDFDPKGKTDDEVMRFCQSFMTELYRHIGEHTDVPAGDIGVGGREIG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
YMFGQYKRITNRWEAGVLTGKGLKFGGSLVRPEATGYGATYFINEALKVRKDSFDGKTCL
HHHHHHHHHHHHHHCCEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
VSGSGNVAIYTIEKIHQLGGKCIACSDSNGVIIHEKGLDVELIKQLKEVERRRIADYATY
EECCCCEEEEEHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
HKDAKYIANGNIWDIPCQVAMPSATQNEINGKDAKTLVKNGCIAVGEGANMPTTPEGIKV
HCCCCEEECCCEEECCCCEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCCEEE
FIEAQIAYGPGKAANAGGVATSALEMQQNAQRDSWSFEETEKKLEKIMVGIHKLCYETAE
EEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EYGDKGNYVTGANIAGFIKVADAMVAHGLV
HHCCCCCEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]