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The map label for this gene is folD2

Identifier: 78222378

GI number: 78222378

Start: 1287679

End: 1288536

Strand: Reverse

Name: folD2

Synonym: Gmet_1162

Alternate gene names: 78222378

Gene position: 1288536-1287679 (Counterclockwise)

Preceding gene: 78222379

Following gene: 78222377

Centisome position: 32.23

GC content: 60.61

Gene sequence:

>858_bases
ATGTCGACAATCATTGACGGAAAAGCCATCGCTGCCAAGCTGAGGGCGGAAATTGCCACCGAAGTCAAGATCCTCAAGGA
AAAGGGGATTATCCCCGGCCTTGCCACGGTCCTTGTGGGGGAAGACCCCGCCAGCGAGGTCTATGTACGGATGAAAGGCA
ATGCCTGCCAGGAGTTGGGGATGCACTCCGTTAAACACACCCTGCCGGCGACTACCACTGAAGCAGAACTCCTCGCTCTC
GTGGCCCGGCTCAACAGCGACCCGACCATCCACGGAATCCTCGTTCAACTCCCCCTCCCGAAACAGATCAATTCCGACAC
GATTCTCGAGGCCATCTCGCCGGTGAAGGACGTAGACGGCTTCCACCCCTACAACGTCGGCCGGCTTATGGTCGGCAAGC
CCACCTTCCAGCCCTGCACCCCCTATGGCGTCATGGTCATGCTGCGCGAAGCGGGCGTTGACCTGGCCGGCAAGGAAGTG
GTCGTCGTGGGGCGCTCAAATATCGTCGGCAAGCCGGTGGCGCTCATGTGCCTCCAGCAGCATGCCACGGTCACCATCTG
CCACTCGAAAACCCGTGATTTGCCATCGAAGGTCAAAGAGGCCGACGTGGTCATCGCCGCCGTGGGGGTTCCCGAGATGA
TCAAGGGGGAGTGGATCAAGGAAGGAGCAGTGGTCATCGATGTGGGAGTCAACCGGGTCGGCGAGAAGAAGCTTGTGGGG
GATGTGGAGTTCGCCGCAGCATCCCAGCGGGCATCGGCCATCACCCCCGTTCCCGGCGGCGTCGGCCCCATGACCATCAC
CATGCTCCTCTACAACACCCTTGAGGCGGCAAAGAAGACAGGGGAAGGAAACCGGTAA

Upstream 100 bases:

>100_bases
AAATCTTTACATCACAGCGACGCTTCCGTACTATTTTGGGATTGTTACCAATCCATTGACGGTGCCGGTTTCGGACCGTT
CCAGGCAAAGGAGCCGTAGC

Downstream 100 bases:

>100_bases
GCGGGGAGCAGCAACGCCCTATGTTTTGCGAGTCCTTGATCCCCGGTCCCGGATTCTTTTATGATTGTCAGCAAACAGAA
ACCCTTTACCGAAATCCTCG

Product: methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSTIIDGKAIAAKLRAEIATEVKILKEKGIIPGLATVLVGEDPASEVYVRMKGNACQELGMHSVKHTLPATTTEAELLAL
VARLNSDPTIHGILVQLPLPKQINSDTILEAISPVKDVDGFHPYNVGRLMVGKPTFQPCTPYGVMVMLREAGVDLAGKEV
VVVGRSNIVGKPVALMCLQQHATVTICHSKTRDLPSKVKEADVVIAAVGVPEMIKGEWIKEGAVVIDVGVNRVGEKKLVG
DVEFAAASQRASAITPVPGGVGPMTITMLLYNTLEAAKKTGEGNR

Sequences:

>Translated_285_residues
MSTIIDGKAIAAKLRAEIATEVKILKEKGIIPGLATVLVGEDPASEVYVRMKGNACQELGMHSVKHTLPATTTEAELLAL
VARLNSDPTIHGILVQLPLPKQINSDTILEAISPVKDVDGFHPYNVGRLMVGKPTFQPCTPYGVMVMLREAGVDLAGKEV
VVVGRSNIVGKPVALMCLQQHATVTICHSKTRDLPSKVKEADVVIAAVGVPEMIKGEWIKEGAVVIDVGVNRVGEKKLVG
DVEFAAASQRASAITPVPGGVGPMTITMLLYNTLEAAKKTGEGNR
>Mature_284_residues
STIIDGKAIAAKLRAEIATEVKILKEKGIIPGLATVLVGEDPASEVYVRMKGNACQELGMHSVKHTLPATTTEAELLALV
ARLNSDPTIHGILVQLPLPKQINSDTILEAISPVKDVDGFHPYNVGRLMVGKPTFQPCTPYGVMVMLREAGVDLAGKEVV
VVGRSNIVGKPVALMCLQQHATVTICHSKTRDLPSKVKEADVVIAAVGVPEMIKGEWIKEGAVVIDVGVNRVGEKKLVGD
VEFAAASQRASAITPVPGGVGPMTITMLLYNTLEAAKKTGEGNR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=47.2222222222222, Blast_Score=261, Evalue=5e-70,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=44.8979591836735, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=44.5205479452055, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI36796743, Length=212, Percent_Identity=32.0754716981132, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1786741, Length=276, Percent_Identity=53.6231884057971, Blast_Score=290, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17568735, Length=263, Percent_Identity=45.2471482889734, Blast_Score=214, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=48.951048951049, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=47.1380471380471, Blast_Score=253, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=47.0588235294118, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=47.0588235294118, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=47.0588235294118, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=47.0588235294118, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI17136816, Length=301, Percent_Identity=46.843853820598, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17136818, Length=301, Percent_Identity=46.843853820598, Blast_Score=251, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD2_GEOMG (Q39WH4)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384125.1
- ProteinModelPortal:   Q39WH4
- SMR:   Q39WH4
- STRING:   Q39WH4
- GeneID:   3740389
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1162
- NMPDR:   fig|269799.3.peg.1730
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- PhylomeDB:   Q39WH4
- ProtClustDB:   CLSK828063
- BioCyc:   GMET269799:GMET_1162-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30180; Mature: 30049

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIIDGKAIAAKLRAEIATEVKILKEKGIIPGLATVLVGEDPASEVYVRMKGNACQELG
CCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHH
MHSVKHTLPATTTEAELLALVARLNSDPTIHGILVQLPLPKQINSDTILEAISPVKDVDG
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHCCHHHCCC
FHPYNVGRLMVGKPTFQPCTPYGVMVMLREAGVDLAGKEVVVVGRSNIVGKPVALMCLQQ
CCCCCCCEEEECCCCCCCCCCHHHHHEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHC
HATVTICHSKTRDLPSKVKEADVVIAAVGVPEMIKGEWIKEGAVVIDVGVNRVGEKKLVG
CCEEEEECCCCCCHHHHHHHCCEEEEECCCCHHHCCCCCCCCCEEEEECCCCCCCHHHCC
DVEFAAASQRASAITPVPGGVGPMTITMLLYNTLEAAKKTGEGNR
CHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STIIDGKAIAAKLRAEIATEVKILKEKGIIPGLATVLVGEDPASEVYVRMKGNACQELG
CCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHH
MHSVKHTLPATTTEAELLALVARLNSDPTIHGILVQLPLPKQINSDTILEAISPVKDVDG
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHCCHHHCCC
FHPYNVGRLMVGKPTFQPCTPYGVMVMLREAGVDLAGKEVVVVGRSNIVGKPVALMCLQQ
CCCCCCCEEEECCCCCCCCCCHHHHHEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHC
HATVTICHSKTRDLPSKVKEADVVIAAVGVPEMIKGEWIKEGAVVIDVGVNRVGEKKLVG
CCEEEEECCCCCCHHHHHHHCCEEEEECCCCHHHCCCCCCCCCEEEEECCCCCCCHHHCC
DVEFAAASQRASAITPVPGGVGPMTITMLLYNTLEAAKKTGEGNR
CHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA