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The map label for this gene is yitJ [H]

Identifier: 78222376

GI number: 78222376

Start: 1286075

End: 1286974

Strand: Reverse

Name: yitJ [H]

Synonym: Gmet_1160

Alternate gene names: 78222376

Gene position: 1286974-1286075 (Counterclockwise)

Preceding gene: 78222377

Following gene: 78222375

Centisome position: 32.2

GC content: 63.44

Gene sequence:

>900_bases
ATGATTACTCCGACCCCCATGTCCTCAACCCTTCGCGAAAAGCTCGAATCAAACCGTTTTGTCATCACGGCCGAGGTCTG
CCCCCCCAAGGGGTGCGACTGCGGCGAGTTCATGGAGAAGGCGCGAAACCTCAAGGGCCTTGTCGACGCCATCAACGTGA
CCGACAACCAGGGGGCGAACGTGCGGATCTCCCCCCTTGCCCCGGCCGCCCTCCTGACCAGGGAAGGGATCGAACCGATC
CTCCAACTCACCTGCCGGGACCGCAACCGCATGGCCCTCCAGAGCGAGCTCCTGGCAGCCGCAGCCCTTGGCGTAACCAA
CATCCTGTCCCTCTCGGGCGATTACATCTCCTTCGGCGATCATGCCGGCGCCAAGCCGGTCTTCGACCTGGACTCGGTGC
AACTTCTCCAGACCATCGCAGCACTGAACGCGGGGAGGGACTTCTCCGGCGCCCCCCTGGAGGGAGCCCCATCGTTCTTC
GCTGGGGCGGCCGCGGCGCCGGAGGCGGACCCCTTCGACCTCACCCTTCCCAAACTGGCCAAAAAAGTATCAGCAGGTGC
GCGCTTCTTCCAGACCCAGGCGGTCTTCTCTCCCGATCGCCTTGCCCGGTTCAGCGAGGCGGTGCGCCCCCTGGGCGTCA
AGATCATCGCCGGCATCATCCTTTTGAAATCGGCCGGCATGGCCCGCTACATCACGAAAAACATCCCCGGACTCAAGGTT
CCCCCCGAGATCGTTGCCGAACTGGAAGCCGCCGGTCGTCCCCTCGAAGTAGGTGTGGCAATCGCCCGGCGGCTTGCGAC
AGCGGCGCGTCCCTTCTGCGACGGTGTCCACATCATGGCCATGGGAAGGGAAGAGCTTGTTCCGAACATCGTGGCCGGAC
TACGAAACGAAGACAAGTAA

Upstream 100 bases:

>100_bases
TCCGCCTTCGGGAGAGGGGCAAGGAGGCCCCGCTCACTGCCATCAAGCCTCCCCGCGACTTCGCCAGGAACCTCAGGCCC
GGAAGCCTGAAACTCGACAA

Downstream 100 bases:

>100_bases
CAAGATCGAGAACGCAAGGAGTGCACCGATGCAGGTAAAAAAGGCAGTCTTCCCCGTGGCGGGCCTCGGCACCCGCTTCC
TTCCGGCCACCAAGGCTTCA

Product: methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MITPTPMSSTLREKLESNRFVITAEVCPPKGCDCGEFMEKARNLKGLVDAINVTDNQGANVRISPLAPAALLTREGIEPI
LQLTCRDRNRMALQSELLAAAALGVTNILSLSGDYISFGDHAGAKPVFDLDSVQLLQTIAALNAGRDFSGAPLEGAPSFF
AGAAAAPEADPFDLTLPKLAKKVSAGARFFQTQAVFSPDRLARFSEAVRPLGVKIIAGIILLKSAGMARYITKNIPGLKV
PPEIVAELEAAGRPLEVGVAIARRLATAARPFCDGVHIMAMGREELVPNIVAGLRNEDK

Sequences:

>Translated_299_residues
MITPTPMSSTLREKLESNRFVITAEVCPPKGCDCGEFMEKARNLKGLVDAINVTDNQGANVRISPLAPAALLTREGIEPI
LQLTCRDRNRMALQSELLAAAALGVTNILSLSGDYISFGDHAGAKPVFDLDSVQLLQTIAALNAGRDFSGAPLEGAPSFF
AGAAAAPEADPFDLTLPKLAKKVSAGARFFQTQAVFSPDRLARFSEAVRPLGVKIIAGIILLKSAGMARYITKNIPGLKV
PPEIVAELEAAGRPLEVGVAIARRLATAARPFCDGVHIMAMGREELVPNIVAGLRNEDK
>Mature_299_residues
MITPTPMSSTLREKLESNRFVITAEVCPPKGCDCGEFMEKARNLKGLVDAINVTDNQGANVRISPLAPAALLTREGIEPI
LQLTCRDRNRMALQSELLAAAALGVTNILSLSGDYISFGDHAGAKPVFDLDSVQLLQTIAALNAGRDFSGAPLEGAPSFF
AGAAAAPEADPFDLTLPKLAKKVSAGARFFQTQAVFSPDRLARFSEAVRPLGVKIIAGIILLKSAGMARYITKNIPGLKV
PPEIVAELEAAGRPLEVGVAIARRLATAARPFCDGVHIMAMGREELVPNIVAGLRNEDK

Specific function: Unknown

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=315, Percent_Identity=26.984126984127, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 31711; Mature: 31711

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITPTPMSSTLREKLESNRFVITAEVCPPKGCDCGEFMEKARNLKGLVDAINVTDNQGAN
CCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
VRISPLAPAALLTREGIEPILQLTCRDRNRMALQSELLAAAALGVTNILSLSGDYISFGD
EEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
HAGAKPVFDLDSVQLLQTIAALNAGRDFSGAPLEGAPSFFAGAAAAPEADPFDLTLPKLA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHH
KKVSAGARFFQTQAVFSPDRLARFSEAVRPLGVKIIAGIILLKSAGMARYITKNIPGLKV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
PPEIVAELEAAGRPLEVGVAIARRLATAARPFCDGVHIMAMGREELVPNIVAGLRNEDK
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHCCEEEEEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MITPTPMSSTLREKLESNRFVITAEVCPPKGCDCGEFMEKARNLKGLVDAINVTDNQGAN
CCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
VRISPLAPAALLTREGIEPILQLTCRDRNRMALQSELLAAAALGVTNILSLSGDYISFGD
EEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
HAGAKPVFDLDSVQLLQTIAALNAGRDFSGAPLEGAPSFFAGAAAAPEADPFDLTLPKLA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHH
KKVSAGARFFQTQAVFSPDRLARFSEAVRPLGVKIIAGIILLKSAGMARYITKNIPGLKV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
PPEIVAELEAAGRPLEVGVAIARRLATAARPFCDGVHIMAMGREELVPNIVAGLRNEDK
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHCCEEEEEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]