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The map label for this gene is nrtB [H]

Identifier: 78222218

GI number: 78222218

Start: 1111835

End: 1112617

Strand: Direct

Name: nrtB [H]

Synonym: Gmet_0998

Alternate gene names: 78222218

Gene position: 1111835-1112617 (Clockwise)

Preceding gene: 78222217

Following gene: 78222222

Centisome position: 27.81

GC content: 60.54

Gene sequence:

>783_bases
ATGAAAAGTGAGCAGACCAAAAGGGCAGCCATTGCCGTGGTGCTCTTCTACGTGCTGATTTTCGCCCTCTGGCAACTGCT
GTTCACAGCCGGCATGATTCCGGAATACCTGTTCCCTTCTCCCGTCCAGGTGGCAAAGCGGTTGTGGGAACTGGGGGCGG
ACGGCTATCTGCTTCCCAGTGTCGAGGCCACTCTCGTGCGGATGGGGAAGGGGTTCACCCTTGCGGCGGCAATCGGGCTG
AGCATCGGCCTTGTCATGGGGGTCAGTCCCATAACCAATCTCTGTTTCAAGTCGCTCTTCCTCGGTCTTCAGACCCTGCC
GACAGCGGCGTGGGTTCCGATCTCCCTCCTCATCTACGGATTGAGCGAGCGGGGGATCTATTTCGTAATTATCATGAGCT
CTGTTCCGGCCATGGCCCTTGCCACGTCGAGCGGCATTCTCCAGATTCCCCCCCTCTACCTCCGGGCGGCGAAAACCCTG
GGCACCCCCTGGTACGCAATGCCCGCGCGGGTGATCCTTCCCGCGGCGCTTCCTGCCATCGTTACGGGCCTCAAGCTGGG
GTGGACCCTGGGGTGGCACGGCGGCGTCTCCGCTGAACTGATCAAATCCACCATCGGCCTTGGTTTCCTGCTCTACATGG
GACGCGAACTGAACGACGCGGCCCAGGTCATGGGTATCATGGTGGTGACGATTCTGTTCGGCCTCCTGCTTGACCGGTTT
TTCTTCGGGATCATCGAGCAGCGGATCCGAATCCGCTGGGGGCTTGAGAAGAGGGCGGGGTAG

Upstream 100 bases:

>100_bases
AAGGAAATCCGCGTGAACCTGCCGCGGCCGAGGGATATCAACGATCAGGAAGTGGGTGAATACGCCAGCCGGATCATGGA
AGAACTGGAGAACCCTCCCG

Downstream 100 bases:

>100_bases
GTTTTGGGAGGACCAGTTACTTAGGCGTCAGATGCCGAGCTGGCGGCACGCCCACTCCGCCGTCCGCCGTACGTTCTCGT
TACCGTCATCGCACAGCTTC

Product: binding-protein dependent transport system inner membrane protein

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKSEQTKRAAIAVVLFYVLIFALWQLLFTAGMIPEYLFPSPVQVAKRLWELGADGYLLPSVEATLVRMGKGFTLAAAIGL
SIGLVMGVSPITNLCFKSLFLGLQTLPTAAWVPISLLIYGLSERGIYFVIIMSSVPAMALATSSGILQIPPLYLRAAKTL
GTPWYAMPARVILPAALPAIVTGLKLGWTLGWHGGVSAELIKSTIGLGFLLYMGRELNDAAQVMGIMVVTILFGLLLDRF
FFGIIEQRIRIRWGLEKRAG

Sequences:

>Translated_260_residues
MKSEQTKRAAIAVVLFYVLIFALWQLLFTAGMIPEYLFPSPVQVAKRLWELGADGYLLPSVEATLVRMGKGFTLAAAIGL
SIGLVMGVSPITNLCFKSLFLGLQTLPTAAWVPISLLIYGLSERGIYFVIIMSSVPAMALATSSGILQIPPLYLRAAKTL
GTPWYAMPARVILPAALPAIVTGLKLGWTLGWHGGVSAELIKSTIGLGFLLYMGRELNDAAQVMGIMVVTILFGLLLDRF
FFGIIEQRIRIRWGLEKRAG
>Mature_260_residues
MKSEQTKRAAIAVVLFYVLIFALWQLLFTAGMIPEYLFPSPVQVAKRLWELGADGYLLPSVEATLVRMGKGFTLAAAIGL
SIGLVMGVSPITNLCFKSLFLGLQTLPTAAWVPISLLIYGLSERGIYFVIIMSSVPAMALATSSGILQIPPLYLRAAKTL
GTPWYAMPARVILPAALPAIVTGLKLGWTLGWHGGVSAELIKSTIGLGFLLYMGRELNDAAQVMGIMVVTILFGLLLDRF
FFGIIEQRIRIRWGLEKRAG

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=203, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081802, Length=235, Percent_Identity=27.6595744680851, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28290; Mature: 28290

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEQTKRAAIAVVLFYVLIFALWQLLFTAGMIPEYLFPSPVQVAKRLWELGADGYLLPS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCEECCC
VEATLVRMGKGFTLAAAIGLSIGLVMGVSPITNLCFKSLFLGLQTLPTAAWVPISLLIYG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LSERGIYFVIIMSSVPAMALATSSGILQIPPLYLRAAKTLGTPWYAMPARVILPAALPAI
HCCCCEEEEEEECCCCHHHHHCCCCCEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VTGLKLGWTLGWHGGVSAELIKSTIGLGFLLYMGRELNDAAQVMGIMVVTILFGLLLDRF
HHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
FFGIIEQRIRIRWGLEKRAG
HHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MKSEQTKRAAIAVVLFYVLIFALWQLLFTAGMIPEYLFPSPVQVAKRLWELGADGYLLPS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCEECCC
VEATLVRMGKGFTLAAAIGLSIGLVMGVSPITNLCFKSLFLGLQTLPTAAWVPISLLIYG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LSERGIYFVIIMSSVPAMALATSSGILQIPPLYLRAAKTLGTPWYAMPARVILPAALPAI
HCCCCEEEEEEECCCCHHHHHCCCCCEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VTGLKLGWTLGWHGGVSAELIKSTIGLGFLLYMGRELNDAAQVMGIMVVTILFGLLLDRF
HHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
FFGIIEQRIRIRWGLEKRAG
HHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7647306 [H]