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The map label for this gene is pmbA [H]

Identifier: 78222167

GI number: 78222167

Start: 1049893

End: 1051230

Strand: Direct

Name: pmbA [H]

Synonym: Gmet_0947

Alternate gene names: 78222167

Gene position: 1049893-1051230 (Clockwise)

Preceding gene: 78222166

Following gene: 78222168

Centisome position: 26.26

GC content: 65.47

Gene sequence:

>1338_bases
ATGAACACAAATGAAATGATCGAAACCGTCGCCACCCTCCTGAAGGACCGTCCCCTGGACGGGTGGGAGGTTGCCGCCGC
AACGTCGCGCCTACTCTCCATCGAGGTGAAGGAGCAGAAGGTGGACACCTTCAAGTGCTCCGCCCCGGGCGGGGTAAGCA
TCCGGGTCCTGAAGAACGGCGGCATGGGGTTCTCGTACTCCACCAGCATGGAGCCTGCCGACCTTGCCCGCATGGTGGAA
AACGCCCTCGTGGGGGCCGGAGCCCAGACCCCCGATGAGCACCATGCCTTTCCCGAGCCCCGTCCCGGTTCGGAGATCCC
CGGGCTCTACGACGAGGGGCTGGCGGCCGTGCCCGAAGCGGAGAAGGTGGCGCGGGCCATGGAGCTGGAGCGGCTCGTCA
TGGCTGCCGACCCGCGGGTGAAGCGGGTGCGCAAATGCTCCTTCGGCGAGTCGACGGTGGAGACCCGTATCGTCAACTCC
CGTGGCGTCGATGCCACCTGCCGGGGTACGTCGGTGTCGGCCAGCGTCTCGGCTATCGCCGAGGAAAGCGGCAATTCCCA
GATGGGGTGGGACTTCGACTTCGCCAACCATTTCACCGATCTGTCCGTTGACCGGATCGCGACACGGGCCGTGGCCAAGG
CGGTGGGGCTTCTGGGGGCCGCGAAGATCCCGACCATGCGCTGTCCGGCGGTTCTCGACAACTACGTGGCCACCGAAATC
CTGGAGGTGCTCGCCTCCGCCTTCCTGGCCGAGAGCGTCCAGAAGGGGAAGTCGCTCCTGGTGGGGAAAAAGGGAACCCG
GATTGTCAGCCCCCTCCTCCGGATTCGGGATGACGGGACCCTCCCCGGCGGCATGGGGACTGCCCCCTTCGATGCCGAGG
GGGTGCCGAAGCAGAACACAATCCTCGTGGAGGACGGCGTGCTCCGTGGCTACCTCTACGACACTCTCCGGGCCCGAAAA
GACGGTGTCGCTTCCACCGGCAATGCCAGCCGCGGCGGCATCAAGTCCCCTCCCCACATGGGAGTGTCCAACCTCTTCAT
CGAGAACGGCACCGTCCCCTTCGATGACCTCATGGCCGGCATCGGGCGGGGGATCCTTCTCACCGACGTCATGGGGATGC
ACACCGCCAATCCCATCTCCGGCGACTTCTCCGTGGGGGCCGCCGGTTTCCTCATCGAAGAGGGGAAGGTGACGGTTCCG
GTCAAGGGAATCGCCATCTCCGGGAATATTCTGGAACTCTTCCAGGGAGTGGAAGGGGTGGGGAACGACCTTCGGCTTTA
CGGCACCGTGGGGGCGCCGTCGCTGCGGATCGCAGCCCTTGATGTGAGCGGCGAGTAG

Upstream 100 bases:

>100_bases
CCACAAGGTGTGGGGCGATTGCATGAAGTGCGTGAAATTCCCCGACTGCGACGAGATCGCAATGATCCTGGACCTCGAAT
AGTCCTCGACAGGTATGCCC

Downstream 100 bases:

>100_bases
GCTGCCATTACAGACCCGGAGTACCGGGCCGGTCAATGAAACCGCCATGTTCGGATTAATACCGGGTGGCGGTTTCGTGA
TTTCAGGTTGACAAACACCG

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MNTNEMIETVATLLKDRPLDGWEVAAATSRLLSIEVKEQKVDTFKCSAPGGVSIRVLKNGGMGFSYSTSMEPADLARMVE
NALVGAGAQTPDEHHAFPEPRPGSEIPGLYDEGLAAVPEAEKVARAMELERLVMAADPRVKRVRKCSFGESTVETRIVNS
RGVDATCRGTSVSASVSAIAEESGNSQMGWDFDFANHFTDLSVDRIATRAVAKAVGLLGAAKIPTMRCPAVLDNYVATEI
LEVLASAFLAESVQKGKSLLVGKKGTRIVSPLLRIRDDGTLPGGMGTAPFDAEGVPKQNTILVEDGVLRGYLYDTLRARK
DGVASTGNASRGGIKSPPHMGVSNLFIENGTVPFDDLMAGIGRGILLTDVMGMHTANPISGDFSVGAAGFLIEEGKVTVP
VKGIAISGNILELFQGVEGVGNDLRLYGTVGAPSLRIAALDVSGE

Sequences:

>Translated_445_residues
MNTNEMIETVATLLKDRPLDGWEVAAATSRLLSIEVKEQKVDTFKCSAPGGVSIRVLKNGGMGFSYSTSMEPADLARMVE
NALVGAGAQTPDEHHAFPEPRPGSEIPGLYDEGLAAVPEAEKVARAMELERLVMAADPRVKRVRKCSFGESTVETRIVNS
RGVDATCRGTSVSASVSAIAEESGNSQMGWDFDFANHFTDLSVDRIATRAVAKAVGLLGAAKIPTMRCPAVLDNYVATEI
LEVLASAFLAESVQKGKSLLVGKKGTRIVSPLLRIRDDGTLPGGMGTAPFDAEGVPKQNTILVEDGVLRGYLYDTLRARK
DGVASTGNASRGGIKSPPHMGVSNLFIENGTVPFDDLMAGIGRGILLTDVMGMHTANPISGDFSVGAAGFLIEEGKVTVP
VKGIAISGNILELFQGVEGVGNDLRLYGTVGAPSLRIAALDVSGE
>Mature_445_residues
MNTNEMIETVATLLKDRPLDGWEVAAATSRLLSIEVKEQKVDTFKCSAPGGVSIRVLKNGGMGFSYSTSMEPADLARMVE
NALVGAGAQTPDEHHAFPEPRPGSEIPGLYDEGLAAVPEAEKVARAMELERLVMAADPRVKRVRKCSFGESTVETRIVNS
RGVDATCRGTSVSASVSAIAEESGNSQMGWDFDFANHFTDLSVDRIATRAVAKAVGLLGAAKIPTMRCPAVLDNYVATEI
LEVLASAFLAESVQKGKSLLVGKKGTRIVSPLLRIRDDGTLPGGMGTAPFDAEGVPKQNTILVEDGVLRGYLYDTLRARK
DGVASTGNASRGGIKSPPHMGVSNLFIENGTVPFDDLMAGIGRGILLTDVMGMHTANPISGDFSVGAAGFLIEEGKVTVP
VKGIAISGNILELFQGVEGVGNDLRLYGTVGAPSLRIAALDVSGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=435, Percent_Identity=27.5862068965517, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1789640, Length=458, Percent_Identity=25.9825327510917, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46817; Mature: 46817

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNEMIETVATLLKDRPLDGWEVAAATSRLLSIEVKEQKVDTFKCSAPGGVSIRVLKNG
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEECCCCCEEEECCCCCCEEEEEECC
GMGFSYSTSMEPADLARMVENALVGAGAQTPDEHHAFPEPRPGSEIPGLYDEGLAAVPEA
CCCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCHHCCCCCCCCH
EKVARAMELERLVMAADPRVKRVRKCSFGESTVETRIVNSRGVDATCRGTSVSASVSAIA
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHEEEECCCCCCCEECCCCCHHHHHHHH
EESGNSQMGWDFDFANHFTDLSVDRIATRAVAKAVGLLGAAKIPTMRCPAVLDNYVATEI
HHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LEVLASAFLAESVQKGKSLLVGKKGTRIVSPLLRIRDDGTLPGGMGTAPFDAEGVPKQNT
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
ILVEDGVLRGYLYDTLRARKDGVASTGNASRGGIKSPPHMGVSNLFIENGTVPFDDLMAG
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
IGRGILLTDVMGMHTANPISGDFSVGAAGFLIEEGKVTVPVKGIAISGNILELFQGVEGV
HCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEECCHHHHHHHHHHCC
GNDLRLYGTVGAPSLRIAALDVSGE
CCCEEEEEECCCCCEEEEEEECCCC
>Mature Secondary Structure
MNTNEMIETVATLLKDRPLDGWEVAAATSRLLSIEVKEQKVDTFKCSAPGGVSIRVLKNG
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEECCCCCEEEECCCCCCEEEEEECC
GMGFSYSTSMEPADLARMVENALVGAGAQTPDEHHAFPEPRPGSEIPGLYDEGLAAVPEA
CCCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCHHCCCCCCCCH
EKVARAMELERLVMAADPRVKRVRKCSFGESTVETRIVNSRGVDATCRGTSVSASVSAIA
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHEEEECCCCCCCEECCCCCHHHHHHHH
EESGNSQMGWDFDFANHFTDLSVDRIATRAVAKAVGLLGAAKIPTMRCPAVLDNYVATEI
HHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LEVLASAFLAESVQKGKSLLVGKKGTRIVSPLLRIRDDGTLPGGMGTAPFDAEGVPKQNT
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
ILVEDGVLRGYLYDTLRARKDGVASTGNASRGGIKSPPHMGVSNLFIENGTVPFDDLMAG
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
IGRGILLTDVMGMHTANPISGDFSVGAAGFLIEEGKVTVPVKGIAISGNILELFQGVEGV
HCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEECCHHHHHHHHHHCC
GNDLRLYGTVGAPSLRIAALDVSGE
CCCEEEEEECCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]