Click here to switch to the map view.

The map label for this gene is gppA [H]

Identifier: 78222100

GI number: 78222100

Start: 968651

End: 970192

Strand: Direct

Name: gppA [H]

Synonym: Gmet_0880

Alternate gene names: 78222100

Gene position: 968651-970192 (Clockwise)

Preceding gene: 78222099

Following gene: 78222109

Centisome position: 24.23

GC content: 66.8

Gene sequence:

>1542_bases
GTGAAGAAGAACCGGCTTGCAGCCATCGATATCGGGACCAACTCCATCCGCTGCATCGTGGTGGAGGTGACGAAGACCGG
CTCCTTCCGCGTCCTGGACGACGAGAAGGCCACGGTGCGCCTGGGGGAGGGGCTGGCCGCCAGCGGTTCCCTCTCGCAGG
CCGCCTGGGAGCGAGCCGTGACGGCTCTCACGCGGATGAAGAAGATCGTGGACGGCTACGGCGTGAAGGCCATCGAGGCG
GTGGCCACGAGCGCGGTTCGGAAGGCCGCCAACGGCGCGGCGTTCATCGCTGCCATCGCCGAGCAGGTGGGGGTTGCGGT
GGTAGTCATCAGCGGCGAGGAGGAGGCGGAGCTGGCGGCCCTCTCGGTGCGGAACCACTTCGACATGGAAGGCGCCCGTT
ACGCCATGGTCGACATCGGCGGCGGGAGCCTGGAGGTGGTGTCGGCCCTTGGCACCCACATCGAGGAGATCTATTCCCTG
GAGCTGGGGGCGGTCTTTCTCACCGAGAAGTTCATGGCGGGGGATTCCGCGCGGCAGCCCGGTCAGGATAAGCTCCGCAA
ATACGTCCGCTCAAGCCTCAAGGAGGTCTTTGCCGGGGAGCGGTCCCAGTTCCAGTGCCTCGTGGGCTCCGGCGGCACCA
TCACCGCGATCGCAGCCATGGTCATGGCCATGCGGGGGGAGGGGTACGGCTCGGTCCACCGCTACGAGGTGCTCCGTTCC
GAGGTGGTGCACCTCCTCGCCATGCTGGCGCGCAAAACGGTAAAGGAGCGGCGGGAGGTGCCGGGGCTCAACCCGGACCG
GGCCGACATCATCGTGGCCGGGGTCACGGTGGTGGACGAGCTCATGCGCTTTTTCGACGCCAACGTTCTGCGGGTCAACG
AGCGGGGAATACGGGAAGGGCTCATCGTCAAGGCGCTTCGCACCCACGGCCTCATCCCCGGCGCGGCGGAGCCCCGGACC
TGGCGCGACGCGGTGCTGGAGTTCGGCCGCTCCTGTCACATGGACGAGGATCATGCCCTCCACGTGACCAAGCTGGCGCT
CAAACTCTTCGACGCGCTGGAGCCGGCCTACGGGCTGGGGGCCGGGGCGCGTCGGATGCTGGAGGCGGCCGCCATCCTCC
ACGACGTGGGGTACTTCATCAACTATTCCAGCCACCACAAGCATTCCTACCATCTCATCCGTCACGCCGACCTCTTCGGC
TTCACCCCTCGGGAGCGGGAGATCATTGCCAACGCGGCCCGCTATCACCGCAAGGCTCTCCCCAAGAAGAAGCACGACGC
CTACGTGCGCCTCGCCGAGCCCGACCGGCAGCTGGTGAGCCGCCTGGGGGGGGTTCTTCGGCTGGCCGACGGCCTCGACC
GGCGCCGCAGCGGTGTCGTCTCCGGCGTCACCTGCTCCGTTTCCAACGGCACGGTCATCGTCACCATCGCCAGCAGCGAG
GATATCTCGGTGGAGCTCTTCGGCGGGAAGATTAAGGGTGATCTCTTCGAGGAGGCGTTCAGGAAGCGGCTTCTCCTCGT
GGCAGAGCACACCCAGAGTTAA

Upstream 100 bases:

>100_bases
ATGCCATTGCCGAGCGGCGCGGGAAGTCTTTTGCCCGCTTCGGGGAACTGCTCGGAGGGCATCCCTTCGAACTGATCGGA
GAAGAGGTGAGGAGAGCGCT

Downstream 100 bases:

>100_bases
GGCGATCGTAACGGCGCCGCAACATGGACCGGCTATGATGGGTAAAACCCTTGATAGGAGGTCCAACATGAACATCAAGC
TCCTCGGTGACTACTACGTC

Product: Ppx/GppA phosphatase

Products: NA

Alternate protein names: Guanosine pentaphosphate phosphohydrolase; pppGpp-5'-phosphohydrolase [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKKNRLAAIDIGTNSIRCIVVEVTKTGSFRVLDDEKATVRLGEGLAASGSLSQAAWERAVTALTRMKKIVDGYGVKAIEA
VATSAVRKAANGAAFIAAIAEQVGVAVVVISGEEEAELAALSVRNHFDMEGARYAMVDIGGGSLEVVSALGTHIEEIYSL
ELGAVFLTEKFMAGDSARQPGQDKLRKYVRSSLKEVFAGERSQFQCLVGSGGTITAIAAMVMAMRGEGYGSVHRYEVLRS
EVVHLLAMLARKTVKERREVPGLNPDRADIIVAGVTVVDELMRFFDANVLRVNERGIREGLIVKALRTHGLIPGAAEPRT
WRDAVLEFGRSCHMDEDHALHVTKLALKLFDALEPAYGLGAGARRMLEAAAILHDVGYFINYSSHHKHSYHLIRHADLFG
FTPREREIIANAARYHRKALPKKKHDAYVRLAEPDRQLVSRLGGVLRLADGLDRRRSGVVSGVTCSVSNGTVIVTIASSE
DISVELFGGKIKGDLFEEAFRKRLLLVAEHTQS

Sequences:

>Translated_513_residues
MKKNRLAAIDIGTNSIRCIVVEVTKTGSFRVLDDEKATVRLGEGLAASGSLSQAAWERAVTALTRMKKIVDGYGVKAIEA
VATSAVRKAANGAAFIAAIAEQVGVAVVVISGEEEAELAALSVRNHFDMEGARYAMVDIGGGSLEVVSALGTHIEEIYSL
ELGAVFLTEKFMAGDSARQPGQDKLRKYVRSSLKEVFAGERSQFQCLVGSGGTITAIAAMVMAMRGEGYGSVHRYEVLRS
EVVHLLAMLARKTVKERREVPGLNPDRADIIVAGVTVVDELMRFFDANVLRVNERGIREGLIVKALRTHGLIPGAAEPRT
WRDAVLEFGRSCHMDEDHALHVTKLALKLFDALEPAYGLGAGARRMLEAAAILHDVGYFINYSSHHKHSYHLIRHADLFG
FTPREREIIANAARYHRKALPKKKHDAYVRLAEPDRQLVSRLGGVLRLADGLDRRRSGVVSGVTCSVSNGTVIVTIASSE
DISVELFGGKIKGDLFEEAFRKRLLLVAEHTQS
>Mature_513_residues
MKKNRLAAIDIGTNSIRCIVVEVTKTGSFRVLDDEKATVRLGEGLAASGSLSQAAWERAVTALTRMKKIVDGYGVKAIEA
VATSAVRKAANGAAFIAAIAEQVGVAVVVISGEEEAELAALSVRNHFDMEGARYAMVDIGGGSLEVVSALGTHIEEIYSL
ELGAVFLTEKFMAGDSARQPGQDKLRKYVRSSLKEVFAGERSQFQCLVGSGGTITAIAAMVMAMRGEGYGSVHRYEVLRS
EVVHLLAMLARKTVKERREVPGLNPDRADIIVAGVTVVDELMRFFDANVLRVNERGIREGLIVKALRTHGLIPGAAEPRT
WRDAVLEFGRSCHMDEDHALHVTKLALKLFDALEPAYGLGAGARRMLEAAAILHDVGYFINYSSHHKHSYHLIRHADLFG
FTPREREIIANAARYHRKALPKKKHDAYVRLAEPDRQLVSRLGGVLRLADGLDRRRSGVVSGVTCSVSNGTVIVTIASSE
DISVELFGGKIKGDLFEEAFRKRLLLVAEHTQS

Specific function: Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in th

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family. GppA subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994966, Length=473, Percent_Identity=29.5983086680761, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788848, Length=468, Percent_Identity=26.4957264957265, Blast_Score=138, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.40 [H]

Molecular weight: Translated: 55849; Mature: 55849

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRLAAIDIGTNSIRCIVVEVTKTGSFRVLDDEKATVRLGEGLAASGSLSQAAWERAV
CCCCCEEEEEECCCCEEEEEEEEECCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHH
TALTRMKKIVDGYGVKAIEAVATSAVRKAANGAAFIAAIAEQVGVAVVVISGEEEAELAA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEECCCCHHHHH
LSVRNHFDMEGARYAMVDIGGGSLEVVSALGTHIEEIYSLELGAVFLTEKFMAGDSARQP
HHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GQDKLRKYVRSSLKEVFAGERSQFQCLVGSGGTITAIAAMVMAMRGEGYGSVHRYEVLRS
CHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EVVHLLAMLARKTVKERREVPGLNPDRADIIVAGVTVVDELMRFFDANVLRVNERGIREG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCCHHH
LIVKALRTHGLIPGAAEPRTWRDAVLEFGRSCHMDEDHALHVTKLALKLFDALEPAYGLG
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AGARRMLEAAAILHDVGYFINYSSHHKHSYHLIRHADLFGFTPREREIIANAARYHRKAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHCCCCCCHHHHHHHHHHHHHHHC
PKKKHDAYVRLAEPDRQLVSRLGGVLRLADGLDRRRSGVVSGVTCSVSNGTVIVTIASSE
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEEECCCEEEEEEECCC
DISVELFGGKIKGDLFEEAFRKRLLLVAEHTQS
CEEEEEECCCCCHHHHHHHHHHHEEEEECCCCC
>Mature Secondary Structure
MKKNRLAAIDIGTNSIRCIVVEVTKTGSFRVLDDEKATVRLGEGLAASGSLSQAAWERAV
CCCCCEEEEEECCCCEEEEEEEEECCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHH
TALTRMKKIVDGYGVKAIEAVATSAVRKAANGAAFIAAIAEQVGVAVVVISGEEEAELAA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEECCCCHHHHH
LSVRNHFDMEGARYAMVDIGGGSLEVVSALGTHIEEIYSLELGAVFLTEKFMAGDSARQP
HHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GQDKLRKYVRSSLKEVFAGERSQFQCLVGSGGTITAIAAMVMAMRGEGYGSVHRYEVLRS
CHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EVVHLLAMLARKTVKERREVPGLNPDRADIIVAGVTVVDELMRFFDANVLRVNERGIREG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCCHHH
LIVKALRTHGLIPGAAEPRTWRDAVLEFGRSCHMDEDHALHVTKLALKLFDALEPAYGLG
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AGARRMLEAAAILHDVGYFINYSSHHKHSYHLIRHADLFGFTPREREIIANAARYHRKAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHCCCCCCHHHHHHHHHHHHHHHC
PKKKHDAYVRLAEPDRQLVSRLGGVLRLADGLDRRRSGVVSGVTCSVSNGTVIVTIASSE
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEEECCCEEEEEEECCC
DISVELFGGKIKGDLFEEAFRKRLLLVAEHTQS
CEEEEEECCCCCHHHHHHHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA