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The map label for this gene is ispH

Identifier: 78222086

GI number: 78222086

Start: 955556

End: 956404

Strand: Direct

Name: ispH

Synonym: Gmet_0866

Alternate gene names: 78222086

Gene position: 955556-956404 (Clockwise)

Preceding gene: 78222085

Following gene: 78222087

Centisome position: 23.9

GC content: 57.36

Gene sequence:

>849_bases
ATGGAAATAATTCTGGCCAAGCGGGCCGGCTTTTGCTTCGGCGTTAAACGGGCCACCCAGATGGCCTTCGAGGCGGCCGA
AAAAGAGGGGAAAACCTACTCCCTCGGTCCCATAATCCACTCGCCTCAGGTGGTGCACCGGCTTGAGGAGATGGGTGTTA
AGGTTCTCAAGGGGCTCGGCGAAATAACCGACGGGACCATCATAGTCCGTTCCCACGGCGTGACTTCGGAGGAGTTGGAA
GAGGCGGTCCGGAAGGAACTTGAAGTCGTCGATGCCACCTGTCCCTTTGTCAAGAAGGCCCAAGAGAATGTTCAGAATCT
CTCCGAGGCCGGTTACGAAGTGGTTGTTGTGGGGGATGCCGATCATCCCGAGGTCCAGGGGATCGTTTCCTACGCCAAGG
GGAAGGTTTGCGTGGTCGGGTCCGAGGAGGAAGCGGCACGGTTGCCGAAGATGAAGAAGATCGGCATAGTCGCCCAGACC
ACTCAATCTTTCGATAATCTGAAGCATGTGGTCCTCGAATGTCTTCGCAAGGGGGCAGAAATTCGCATCTTCAACACCAT
CTGTGATGCGACGGCCGTGCGCCAGCAGGAGGCGACGGCTTTGGCCCGCACGGTTGACTGCATGATCGTTATCGGTGGCT
ACAACAGCGCCAATACCAAACGCCTCGCCGAGGTCTGCGCTGAACTCCAACCCCGGACCCATCAGATTGAGACGGCCCAG
GAGATCAACCCTGCCTGGTTCGAGGGGGTAGCCAAGGTGGGGGTGACCGCCGGAGCGTCAACTCCCAAGTGGCTCATCGA
TGAGGTGATCGAGCGGATCAGAGAGATTGATAGTGCTAAAACTAGTTGA

Upstream 100 bases:

>100_bases
CGCCATCATGGAGGGGATGGTCCGGGAAAAGGAGCAGGCCCACAGCGTCTGCGCCCCCTGAAAAACCAACGAATAAAGAA
CACTCTGGAGTCTCAATCAT

Downstream 100 bases:

>100_bases
ATTTTCAAATAGATATGTGTTACGTTTGCATATCCTAATCCACAAACGATTCAGGGAGGCCAGCTTTAATGAGTGAAGAC
AAGGACATGAAGAACCGCAA

Product: LytB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MEIILAKRAGFCFGVKRATQMAFEAAEKEGKTYSLGPIIHSPQVVHRLEEMGVKVLKGLGEITDGTIIVRSHGVTSEELE
EAVRKELEVVDATCPFVKKAQENVQNLSEAGYEVVVVGDADHPEVQGIVSYAKGKVCVVGSEEEAARLPKMKKIGIVAQT
TQSFDNLKHVVLECLRKGAEIRIFNTICDATAVRQQEATALARTVDCMIVIGGYNSANTKRLAEVCAELQPRTHQIETAQ
EINPAWFEGVAKVGVTAGASTPKWLIDEVIERIREIDSAKTS

Sequences:

>Translated_282_residues
MEIILAKRAGFCFGVKRATQMAFEAAEKEGKTYSLGPIIHSPQVVHRLEEMGVKVLKGLGEITDGTIIVRSHGVTSEELE
EAVRKELEVVDATCPFVKKAQENVQNLSEAGYEVVVVGDADHPEVQGIVSYAKGKVCVVGSEEEAARLPKMKKIGIVAQT
TQSFDNLKHVVLECLRKGAEIRIFNTICDATAVRQQEATALARTVDCMIVIGGYNSANTKRLAEVCAELQPRTHQIETAQ
EINPAWFEGVAKVGVTAGASTPKWLIDEVIERIREIDSAKTS
>Mature_282_residues
MEIILAKRAGFCFGVKRATQMAFEAAEKEGKTYSLGPIIHSPQVVHRLEEMGVKVLKGLGEITDGTIIVRSHGVTSEELE
EAVRKELEVVDATCPFVKKAQENVQNLSEAGYEVVVVGDADHPEVQGIVSYAKGKVCVVGSEEEAARLPKMKKIGIVAQT
TQSFDNLKHVVLECLRKGAEIRIFNTICDATAVRQQEATALARTVDCMIVIGGYNSANTKRLAEVCAELQPRTHQIETAQ
EINPAWFEGVAKVGVTAGASTPKWLIDEVIERIREIDSAKTS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=286, Percent_Identity=35.3146853146853, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_GEOMG (Q39XB6)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383833.1
- STRING:   Q39XB6
- GeneID:   3739295
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0866
- NMPDR:   fig|269799.3.peg.941
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   Q39XB6
- ProtClustDB:   CLSK790176
- BioCyc:   GMET269799:GMET_0866-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 30731; Mature: 30731

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIILAKRAGFCFGVKRATQMAFEAAEKEGKTYSLGPIIHSPQVVHRLEEMGVKVLKGLG
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCC
EITDGTIIVRSHGVTSEELEEAVRKELEVVDATCPFVKKAQENVQNLSEAGYEVVVVGDA
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCC
DHPEVQGIVSYAKGKVCVVGSEEEAARLPKMKKIGIVAQTTQSFDNLKHVVLECLRKGAE
CCCHHHHHHHHCCCCEEEECCCHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
IRIFNTICDATAVRQQEATALARTVDCMIVIGGYNSANTKRLAEVCAELQPRTHQIETAQ
EEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHH
EINPAWFEGVAKVGVTAGASTPKWLIDEVIERIREIDSAKTS
HCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEIILAKRAGFCFGVKRATQMAFEAAEKEGKTYSLGPIIHSPQVVHRLEEMGVKVLKGLG
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCC
EITDGTIIVRSHGVTSEELEEAVRKELEVVDATCPFVKKAQENVQNLSEAGYEVVVVGDA
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCC
DHPEVQGIVSYAKGKVCVVGSEEEAARLPKMKKIGIVAQTTQSFDNLKHVVLECLRKGAE
CCCHHHHHHHHCCCCEEEECCCHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
IRIFNTICDATAVRQQEATALARTVDCMIVIGGYNSANTKRLAEVCAELQPRTHQIETAQ
EEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHH
EINPAWFEGVAKVGVTAGASTPKWLIDEVIERIREIDSAKTS
HCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA