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The map label for this gene is rlmI [H]

Identifier: 78222065

GI number: 78222065

Start: 930371

End: 931564

Strand: Reverse

Name: rlmI [H]

Synonym: Gmet_0845

Alternate gene names: 78222065

Gene position: 931564-930371 (Counterclockwise)

Preceding gene: 78222066

Following gene: 78222061

Centisome position: 23.3

GC content: 64.32

Gene sequence:

>1194_bases
ATGACCATGGAAACGACCACTATCCGCGTGACCCTCAACCGTGGCGAAGACCGCCGAATCAAGGGAGGCCACCCCTGGGT
CTTCAGTAACGAGATCCGGGAGGCAAAGGGGGAACGGATCCCCGGCGCCACCGCCGAGGTCTACGATGTGGGGGGCGGGT
TTCTCGGCACCGGCTACTACAACCCCCACTCCCTCATCGCGGTGCGGCTCCTCTCCCGGGAGCGGGAGGAGATCGACTCG
CCGGCGTTTTTCAAGGAGCGAATCAACCAGGCGTTGAAACTGCGTCGCAGCCTCTACCCGGACATGGCCACCTTCCGGGT
CGTCCACGGCGAAGGGGACTTTCTCCCCGGTCTCGTCGTCGACAAGTACGGCGACCGGCTTTCGGTCCAGATACTGACCG
CGGGGATAGAGGCACGCCGGGGAGCCATCGTGGAGGCCCTCGTGGAACTTTTCGCCCCCAAGGGGATCATCGCCCGCAAC
GACGTGGCCGTGCGGTCACTGGAGGGGCTGGCGGAAACCGTGGAAATTTTGTATAGAGAGATCCCGGAGACGGTGGAGGC
GGAGGAGCACGGTCTGCGCTTCCGGGTCGACCTGGCCGGAGGCCAGAAGACTGGCCACTTTCTGGACCAGAAGGAAAACC
ATCTCGTACTGAAGGGAATGGCTGCAGGAAAGAGGGTTCTGGACTGTTTCTCCTACTCGGGGAGTTGGGGAGTCCATGCC
GCGGCCTTTGGTGCCGCGGAAGTGACCTGCCTCGACATCTCGGAGCGGGCCGCGGCCCTTGCCCGGGAGAACGCGGCCCT
GAACGGGCTTGAAGGAGTGGTGAGCGTGGAGGTCTGCGACGCCTTTGACCGGCTCCGGAGCCTGAGGCACGAGGGGCGGC
GCTTCGACGTGGTGGTCCTCGATCCCCCTGCCTTCGTGAAGAACAAGAAGGCGTTGAAGGAGGCGGAGAAGGGATATCTC
ACCATCAATCGGCGTGGGTTGGAACTCCTCGACGCTGGAGGGTACCTGATCACCTGCTCCTGTTCCTACCACATGGGGCG
GGAGTTGTTCAGGGATCTCCTGGCCCAGGCGGCCCGCCAGGCGGGGCGTCAGGTGCGGATCGTGGAGACCCGCTCCCAGG
CCCCGGATCACCCCGCGCTCCTGGCCGTGCCGGAGACCGAGTACCTCAAGTGTTTCGTGCTGCAGTGCGTGTAA

Upstream 100 bases:

>100_bases
CATCCCCTGGCCAAGAAACTCATCGAGGTTCTCGGCTGAACGGCAGCCTGCATACCGACACCACACGAATCCCCTCCCGG
CGAGGGGATTTTTTTTGGAG

Downstream 100 bases:

>100_bases
CGAAGCGAGATGGTGAGATAGCGAGATAGCAAGCAGCAAAACCATCTCGCTACCTCGCTACTTGCGCCCACTCTTCACTC
CCACCCCGTAAATCACCGTG

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTMETTTIRVTLNRGEDRRIKGGHPWVFSNEIREAKGERIPGATAEVYDVGGGFLGTGYYNPHSLIAVRLLSREREEIDS
PAFFKERINQALKLRRSLYPDMATFRVVHGEGDFLPGLVVDKYGDRLSVQILTAGIEARRGAIVEALVELFAPKGIIARN
DVAVRSLEGLAETVEILYREIPETVEAEEHGLRFRVDLAGGQKTGHFLDQKENHLVLKGMAAGKRVLDCFSYSGSWGVHA
AAFGAAEVTCLDISERAAALARENAALNGLEGVVSVEVCDAFDRLRSLRHEGRRFDVVVLDPPAFVKNKKALKEAEKGYL
TINRRGLELLDAGGYLITCSCSYHMGRELFRDLLAQAARQAGRQVRIVETRSQAPDHPALLAVPETEYLKCFVLQCV

Sequences:

>Translated_397_residues
MTMETTTIRVTLNRGEDRRIKGGHPWVFSNEIREAKGERIPGATAEVYDVGGGFLGTGYYNPHSLIAVRLLSREREEIDS
PAFFKERINQALKLRRSLYPDMATFRVVHGEGDFLPGLVVDKYGDRLSVQILTAGIEARRGAIVEALVELFAPKGIIARN
DVAVRSLEGLAETVEILYREIPETVEAEEHGLRFRVDLAGGQKTGHFLDQKENHLVLKGMAAGKRVLDCFSYSGSWGVHA
AAFGAAEVTCLDISERAAALARENAALNGLEGVVSVEVCDAFDRLRSLRHEGRRFDVVVLDPPAFVKNKKALKEAEKGYL
TINRRGLELLDAGGYLITCSCSYHMGRELFRDLLAQAARQAGRQVRIVETRSQAPDHPALLAVPETEYLKCFVLQCV
>Mature_396_residues
TMETTTIRVTLNRGEDRRIKGGHPWVFSNEIREAKGERIPGATAEVYDVGGGFLGTGYYNPHSLIAVRLLSREREEIDSP
AFFKERINQALKLRRSLYPDMATFRVVHGEGDFLPGLVVDKYGDRLSVQILTAGIEARRGAIVEALVELFAPKGIIARND
VAVRSLEGLAETVEILYREIPETVEAEEHGLRFRVDLAGGQKTGHFLDQKENHLVLKGMAAGKRVLDCFSYSGSWGVHAA
AFGAAEVTCLDISERAAALARENAALNGLEGVVSVEVCDAFDRLRSLRHEGRRFDVVVLDPPAFVKNKKALKEAEKGYLT
INRRGLELLDAGGYLITCSCSYHMGRELFRDLLAQAARQAGRQVRIVETRSQAPDHPALLAVPETEYLKCFVLQCV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=391, Percent_Identity=39.386189258312, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1787181, Length=234, Percent_Identity=29.4871794871795, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43936; Mature: 43805

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMETTTIRVTLNRGEDRRIKGGHPWVFSNEIREAKGERIPGATAEVYDVGGGFLGTGYY
CCCEEEEEEEEEECCCCCCCCCCCCEEECCHHHHHCCCCCCCCCCEEEECCCCEEECCCC
NPHSLIAVRLLSREREEIDSPAFFKERINQALKLRRSLYPDMATFRVVHGEGDFLPGLVV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCE
DKYGDRLSVQILTAGIEARRGAIVEALVELFAPKGIIARNDVAVRSLEGLAETVEILYRE
ECCCCEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHH
IPETVEAEEHGLRFRVDLAGGQKTGHFLDQKENHLVLKGMAAGKRVLDCFSYSGSWGVHA
CCHHHCHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCHH
AAFGAAEVTCLDISERAAALARENAALNGLEGVVSVEVCDAFDRLRSLRHEGRRFDVVVL
EECCCCEEEEEEHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEE
DPPAFVKNKKALKEAEKGYLTINRRGLELLDAGGYLITCSCSYHMGRELFRDLLAQAARQ
CCCHHHCCHHHHHHHHCCEEEEECCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHH
AGRQVRIVETRSQAPDHPALLAVPETEYLKCFVLQCV
CCCEEEEEECCCCCCCCCEEEECCCHHHHHHEEEECC
>Mature Secondary Structure 
TMETTTIRVTLNRGEDRRIKGGHPWVFSNEIREAKGERIPGATAEVYDVGGGFLGTGYY
CCEEEEEEEEEECCCCCCCCCCCCEEECCHHHHHCCCCCCCCCCEEEECCCCEEECCCC
NPHSLIAVRLLSREREEIDSPAFFKERINQALKLRRSLYPDMATFRVVHGEGDFLPGLVV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCE
DKYGDRLSVQILTAGIEARRGAIVEALVELFAPKGIIARNDVAVRSLEGLAETVEILYRE
ECCCCEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHH
IPETVEAEEHGLRFRVDLAGGQKTGHFLDQKENHLVLKGMAAGKRVLDCFSYSGSWGVHA
CCHHHCHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCHH
AAFGAAEVTCLDISERAAALARENAALNGLEGVVSVEVCDAFDRLRSLRHEGRRFDVVVL
EECCCCEEEEEEHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEE
DPPAFVKNKKALKEAEKGYLTINRRGLELLDAGGYLITCSCSYHMGRELFRDLLAQAARQ
CCCHHHCCHHHHHHHHCCEEEEECCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHH
AGRQVRIVETRSQAPDHPALLAVPETEYLKCFVLQCV
CCCEEEEEECCCCCCCCCEEEECCCHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA