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The map label for this gene is ycdT [H]

Identifier: 78222060

GI number: 78222060

Start: 925363

End: 926598

Strand: Reverse

Name: ycdT [H]

Synonym: Gmet_0840

Alternate gene names: 78222060

Gene position: 926598-925363 (Counterclockwise)

Preceding gene: 78222061

Following gene: 78222059

Centisome position: 23.18

GC content: 63.92

Gene sequence:

>1236_bases
ATGCATGCTCTTTCAATGAAAAACCTGACCATTTTCCTTATTCCCGCTGCGTTTCTCACAGCCACCATCTGGTTCTTCCC
CCAGGTGGCAACGCTACCGCCGGGGCGGCTGGAGCTTCTCCCCTTCATTCCCTATCTGACCATGGCTTTGGGCATGATGC
TCTCGTTCCATTTTCACCGGGGGAGGGCATTTTTCGTTTTTCTCGTTCTGATTGCCGTTTACTGGAGCTTCCGGGGGCAC
CTGAACGGCGCTCCGACCGGAATCACGGCCACGGTCCTGTTCCAGTCCGTTTGCGTTCTTGTCCCCCTGAACATCGCGCT
CTTCTCCCTCATGCGCGAGCGGGGGATCATGACCGTTGCCGGCCGGCTCCGTTTCGCCTTCCTGGCGCTCCAGGCCCTTT
TCGTGGGATGGGCCATGGAACCGGGGCATGTGGCGGTCCAGCAACTTCTCTCCTGCCGGTTCACCGAGAGCCACCTCCTG
GCTGGGCTCCAGCTTCCCCAGCCGGCACTGCCGGTAATCGTCCTCGGGACGGCCGTGGTAATCGGTCGGGCCGCCAGGGG
CCAGTCTCCCATAGACGCAAGCCTTCTGGGAGTCATGGCGGCCTTTGTGGTGGCCTGCAACGGCATCGGGACAGAGTTCG
TCACGCCGGTTTTCATGGCTGCCGCCGCCGCCATCCTCACCCTCGGGGTTTTGAAGGACTCCTACAACATGGCCTTCAGG
GACGAGCTGACCGGCCTCCCCTCCCGCCGCGCCCTCAACGAGCAGCTCACATGGCTCGGGCGGCGCTACACCGTGGCCAT
GGTGGACGTGGACCATTTCAAGAGGTTCAACGACACCTACGGCCACGACGTGGGGGACCAGGTGCTGCGGATGGTTGCAT
CGAAGCTGCGGGGCGTGGGCGGGGGCGGAAAGGTCTACCGCTACGGCGGTGAAGAGTTCACTATCCTCTTCCACCGCAAG
GAGCGAGAGGAGGTTCTCGCCGACCTGGAAGGGCTGCGCCGCACCATTGCCGACTACCAGATGCGCCTGCGCGGCACCGA
CCGCCCCAGTTCGTCCAAAGAGGGGAAAAAACTCCGCTACGGCACCGGACGCGGCAACGCCACCGTCTCAGTGACCGTCA
GCATCGGCGTTGCCGAGAGCAGCGCCGACCGCCGCCGCCCCGCCGACGTCATCAAGGCGGCCGACCAGGCCCTCTACCGG
GCCAAAGGTGGCGGTCGCAACCGCGTCTGCAGCTGA

Upstream 100 bases:

>100_bases
ACCCTCTCTTTTCAGGAAAATATTGATTAAGCCTTCTAAAGTTTTCTCATTTTTCTCCGATATGTTTGCTTAGGACCCAT
CAACCAGGAGCCTTTGCAGC

Downstream 100 bases:

>100_bases
CGGCACGCCCCCCCTCCATTCACTCCTTCTCATCCTCCCCCCCGTTGCATTCGTTTCCCTCCCCGCTATACTTTTTCCGT
TACTCACCAAACAAGTACGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MHALSMKNLTIFLIPAAFLTATIWFFPQVATLPPGRLELLPFIPYLTMALGMMLSFHFHRGRAFFVFLVLIAVYWSFRGH
LNGAPTGITATVLFQSVCVLVPLNIALFSLMRERGIMTVAGRLRFAFLALQALFVGWAMEPGHVAVQQLLSCRFTESHLL
AGLQLPQPALPVIVLGTAVVIGRAARGQSPIDASLLGVMAAFVVACNGIGTEFVTPVFMAAAAAILTLGVLKDSYNMAFR
DELTGLPSRRALNEQLTWLGRRYTVAMVDVDHFKRFNDTYGHDVGDQVLRMVASKLRGVGGGGKVYRYGGEEFTILFHRK
EREEVLADLEGLRRTIADYQMRLRGTDRPSSSKEGKKLRYGTGRGNATVSVTVSIGVAESSADRRRPADVIKAADQALYR
AKGGGRNRVCS

Sequences:

>Translated_411_residues
MHALSMKNLTIFLIPAAFLTATIWFFPQVATLPPGRLELLPFIPYLTMALGMMLSFHFHRGRAFFVFLVLIAVYWSFRGH
LNGAPTGITATVLFQSVCVLVPLNIALFSLMRERGIMTVAGRLRFAFLALQALFVGWAMEPGHVAVQQLLSCRFTESHLL
AGLQLPQPALPVIVLGTAVVIGRAARGQSPIDASLLGVMAAFVVACNGIGTEFVTPVFMAAAAAILTLGVLKDSYNMAFR
DELTGLPSRRALNEQLTWLGRRYTVAMVDVDHFKRFNDTYGHDVGDQVLRMVASKLRGVGGGGKVYRYGGEEFTILFHRK
EREEVLADLEGLRRTIADYQMRLRGTDRPSSSKEGKKLRYGTGRGNATVSVTVSIGVAESSADRRRPADVIKAADQALYR
AKGGGRNRVCS
>Mature_411_residues
MHALSMKNLTIFLIPAAFLTATIWFFPQVATLPPGRLELLPFIPYLTMALGMMLSFHFHRGRAFFVFLVLIAVYWSFRGH
LNGAPTGITATVLFQSVCVLVPLNIALFSLMRERGIMTVAGRLRFAFLALQALFVGWAMEPGHVAVQQLLSCRFTESHLL
AGLQLPQPALPVIVLGTAVVIGRAARGQSPIDASLLGVMAAFVVACNGIGTEFVTPVFMAAAAAILTLGVLKDSYNMAFR
DELTGLPSRRALNEQLTWLGRRYTVAMVDVDHFKRFNDTYGHDVGDQVLRMVASKLRGVGGGGKVYRYGGEEFTILFHRK
EREEVLADLEGLRRTIADYQMRLRGTDRPSSSKEGKKLRYGTGRGNATVSVTVSIGVAESSADRRRPADVIKAADQALYR
AKGGGRNRVCS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=33.5135135135135, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693134, Length=149, Percent_Identity=36.241610738255, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787816, Length=175, Percent_Identity=34.8571428571429, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787056, Length=180, Percent_Identity=31.6666666666667, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786584, Length=180, Percent_Identity=30, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787802, Length=210, Percent_Identity=31.4285714285714, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=31.7919075144509, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=29.4444444444444, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082007, Length=185, Percent_Identity=29.7297297297297, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45039; Mature: 45039

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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CCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]