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The map label for this gene is ruvC

Identifier: 78221964

GI number: 78221964

Start: 818496

End: 818990

Strand: Direct

Name: ruvC

Synonym: Gmet_0744

Alternate gene names: 78221964

Gene position: 818496-818990 (Clockwise)

Preceding gene: 78221963

Following gene: 78221965

Centisome position: 20.48

GC content: 63.23

Gene sequence:

>495_bases
ATGAGAGTTCTCGGCATTGACCCCGGGTCGCGCATCACCGGCTACGGCATCATAGAAAAGGTCGGCAACCGCCTCGTCCA
TGTGGACAACGGCGCCATCCATACCGATAACCACAAGGAGTTCGCGCTCCGCCTCCACAGGATATTCGAAGGACTCTGCG
AGGTTATCGCAGAATACCGTCCCGACGCCATGGCCGTGGAGCAGGTGTTCCTCGCCCACAACGCCCAGAGCGCCCTGAAG
CTGGGGCAGGCCCGGGGGGCCGCCATCGTGGCCGGGGTGAACGCGGGGCTCCCGGTCAGCGAGTACACGGCCATGCAGGT
CAAACAGGCGGTTGTCGGCTACGGCCATGCCCGCAAGGAGCAGGTGCAGCAGATGGTCAAGTCCCTCCTTAACCTTCCTG
AGATCGCCCAGGCCGACGCCTCCGACGCATTGGCCGTGGCGGTCTGTCACGCCAATTCGGCCGGCATGAAAAACATCCTC
CGGAGCATCAGATGA

Upstream 100 bases:

>100_bases
TTACATGCATGCTTATTCAAGCGCGGAAGCCGGAAGCCAGCCTTTCCTTCCGCGTTTTTTCTCTGTGTTCTCTGTGCCCT
CTGTGGCAGAATGCTTCCCC

Downstream 100 bases:

>100_bases
TAGCCCGTCTCACCGGCATGCTCGCCCACAAGTCCCCCGACGCCATCATCATCGACGTGAACGGTGTCGGCTACCGGGTC
CAGATCCCCTTTTCCACCTA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MRVLGIDPGSRITGYGIIEKVGNRLVHVDNGAIHTDNHKEFALRLHRIFEGLCEVIAEYRPDAMAVEQVFLAHNAQSALK
LGQARGAAIVAGVNAGLPVSEYTAMQVKQAVVGYGHARKEQVQQMVKSLLNLPEIAQADASDALAVAVCHANSAGMKNIL
RSIR

Sequences:

>Translated_164_residues
MRVLGIDPGSRITGYGIIEKVGNRLVHVDNGAIHTDNHKEFALRLHRIFEGLCEVIAEYRPDAMAVEQVFLAHNAQSALK
LGQARGAAIVAGVNAGLPVSEYTAMQVKQAVVGYGHARKEQVQQMVKSLLNLPEIAQADASDALAVAVCHANSAGMKNIL
RSIR
>Mature_164_residues
MRVLGIDPGSRITGYGIIEKVGNRLVHVDNGAIHTDNHKEFALRLHRIFEGLCEVIAEYRPDAMAVEQVFLAHNAQSALK
LGQARGAAIVAGVNAGLPVSEYTAMQVKQAVVGYGHARKEQVQQMVKSLLNLPEIAQADASDALAVAVCHANSAGMKNIL
RSIR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=157, Percent_Identity=56.0509554140127, Blast_Score=154, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_GEOMG (Q39XN8)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383711.1
- ProteinModelPortal:   Q39XN8
- SMR:   Q39XN8
- STRING:   Q39XN8
- GeneID:   3740550
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0744
- NMPDR:   fig|269799.3.peg.771
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q39XN8
- ProtClustDB:   PRK00039
- BioCyc:   GMET269799:GMET_0744-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17633; Mature: 17633

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLGIDPGSRITGYGIIEKVGNRLVHVDNGAIHTDNHKEFALRLHRIFEGLCEVIAEYR
CEEECCCCCCCCHHHHHHHHHCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHC
PDAMAVEQVFLAHNAQSALKLGQARGAAIVAGVNAGLPVSEYTAMQVKQAVVGYGHARKE
CCHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHH
QVQQMVKSLLNLPEIAQADASDALAVAVCHANSAGMKNILRSIR
HHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MRVLGIDPGSRITGYGIIEKVGNRLVHVDNGAIHTDNHKEFALRLHRIFEGLCEVIAEYR
CEEECCCCCCCCHHHHHHHHHCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHC
PDAMAVEQVFLAHNAQSALKLGQARGAAIVAGVNAGLPVSEYTAMQVKQAVVGYGHARKE
CCHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHH
QVQQMVKSLLNLPEIAQADASDALAVAVCHANSAGMKNILRSIR
HHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA