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The map label for this gene is yugF [H]

Identifier: 78221946

GI number: 78221946

Start: 799236

End: 800129

Strand: Reverse

Name: yugF [H]

Synonym: Gmet_0726

Alternate gene names: 78221946

Gene position: 800129-799236 (Counterclockwise)

Preceding gene: 78221947

Following gene: 78221944

Centisome position: 20.02

GC content: 66.22

Gene sequence:

>894_bases
ATGGGCGACAGGCTCACCTTCGTCTACGGTCCCGGCGTCGACATCAGCTATCGCGTTGTCGGCCACGGCCCGACGCCGGT
CGTCTTTGTCCACGGCTTCGCGGCTGCCCGCACCACGTGGAATGACATCGTCCCCCTTTTCCCGGCAGAGCGTTTCACCC
TCTATCTTATCGACCTGAAAGGGTTCGGCTTTTCCTCCAAGCCCCGCACCGGCTCCTACGCCATCGAGGAGCAGGCCGCG
GTGACCACTGCATTCCTGAAGGCAAAGGGACTTTCGCGTGTCATCCTGACCGGGCATTCCCTCGGCGGCGCCATCGCCCT
CCTCGTCACCCTCCAGGCACGGGACCGGGGAGACACGGGCCTCGTAGCGCGGCTCATCCTCGTCGCCTGTTCCGCCTACC
CCCAGAAGCTCCCGCGGCTCATGGGGTGGCTCCGGATTCCGTTCCTGGCACGTATCGGCATGGCCCTCATCCCGGTGCGC
ACCATTGTCCGCTACACCCTCGCCCGGGTCTTCCACGACACGAGGGCCATCACTCCCGAGCGGATCAGGCGTTACGAGCA
CTGCTTCGGCCGGCGGGGCATGGCAGGGGTCCTGATCCGGAGCGCCCGGGCCATCGACCCGGACAGCTACGGCGCGATCA
CGGCCCGCTACCGGGAGATCGACATACCTACCCTGATTGTCTGGGGAAAGGAGGACCGGATCGTGCGGATCGGCCAGGGG
AAGCGGCTCGCGGAAGAGATGCCGGATGCCCGGCTCGCGGTGATCGATGGCTGCGGCCACAACCCCCACGAAGAGCGTCC
CCGCGAGACCCTTGCGGCGATTATGGAGTTTCTGGGGAACGGAGAGGAGCGGGAGGAAGGGAGTGGCCGGTTCTGGCGTG
AAGCTCGTCGATGA

Upstream 100 bases:

>100_bases
AAGCTCCTGCTTGGCACCTGGCAATCCATCTACCTCTGCGAGTTCGACGGGCCGCGCCAGCGCAAGGTTGCCCTCAAGAT
CGTCTCCTGCTGACGCCGCC

Downstream 100 bases:

>100_bases
AATGCCGGTAGGCCATTTCGCCGGCGATGAACTTCGTGATCATGACCCCCATGCCGCTCTTCTTCACGAGATGGATCATT
TGAGGAGGGACCTTCTTGGT

Product: Alpha/beta hydrolase fold

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MGDRLTFVYGPGVDISYRVVGHGPTPVVFVHGFAAARTTWNDIVPLFPAERFTLYLIDLKGFGFSSKPRTGSYAIEEQAA
VTTAFLKAKGLSRVILTGHSLGGAIALLVTLQARDRGDTGLVARLILVACSAYPQKLPRLMGWLRIPFLARIGMALIPVR
TIVRYTLARVFHDTRAITPERIRRYEHCFGRRGMAGVLIRSARAIDPDSYGAITARYREIDIPTLIVWGKEDRIVRIGQG
KRLAEEMPDARLAVIDGCGHNPHEERPRETLAAIMEFLGNGEEREEGSGRFWREARR

Sequences:

>Translated_297_residues
MGDRLTFVYGPGVDISYRVVGHGPTPVVFVHGFAAARTTWNDIVPLFPAERFTLYLIDLKGFGFSSKPRTGSYAIEEQAA
VTTAFLKAKGLSRVILTGHSLGGAIALLVTLQARDRGDTGLVARLILVACSAYPQKLPRLMGWLRIPFLARIGMALIPVR
TIVRYTLARVFHDTRAITPERIRRYEHCFGRRGMAGVLIRSARAIDPDSYGAITARYREIDIPTLIVWGKEDRIVRIGQG
KRLAEEMPDARLAVIDGCGHNPHEERPRETLAAIMEFLGNGEEREEGSGRFWREARR
>Mature_296_residues
GDRLTFVYGPGVDISYRVVGHGPTPVVFVHGFAAARTTWNDIVPLFPAERFTLYLIDLKGFGFSSKPRTGSYAIEEQAAV
TTAFLKAKGLSRVILTGHSLGGAIALLVTLQARDRGDTGLVARLILVACSAYPQKLPRLMGWLRIPFLARIGMALIPVRT
IVRYTLARVFHDTRAITPERIRRYEHCFGRRGMAGVLIRSARAIDPDSYGAITARYREIDIPTLIVWGKEDRIVRIGQGK
RLAEEMPDARLAVIDGCGHNPHEERPRETLAAIMEFLGNGEEREEGSGRFWREARR

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33126; Mature: 32995

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDRLTFVYGPGVDISYRVVGHGPTPVVFVHGFAAARTTWNDIVPLFPAERFTLYLIDLK
CCCEEEEEECCCCCEEEEEEECCCCCEEEEECCHHHHCCHHCCCCCCCCCCEEEEEEEEE
GFGFSSKPRTGSYAIEEQAAVTTAFLKAKGLSRVILTGHSLGGAIALLVTLQARDRGDTG
CCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCCCHH
LVARLILVACSAYPQKLPRLMGWLRIPFLARIGMALIPVRTIVRYTLARVFHDTRAITPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RIRRYEHCFGRRGMAGVLIRSARAIDPDSYGAITARYREIDIPTLIVWGKEDRIVRIGQG
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEECCCCCEEEECCC
KRLAEEMPDARLAVIDGCGHNPHEERPRETLAAIMEFLGNGEEREEGSGRFWREARR
CHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
GDRLTFVYGPGVDISYRVVGHGPTPVVFVHGFAAARTTWNDIVPLFPAERFTLYLIDLK
CCEEEEEECCCCCEEEEEEECCCCCEEEEECCHHHHCCHHCCCCCCCCCCEEEEEEEEE
GFGFSSKPRTGSYAIEEQAAVTTAFLKAKGLSRVILTGHSLGGAIALLVTLQARDRGDTG
CCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCCCHH
LVARLILVACSAYPQKLPRLMGWLRIPFLARIGMALIPVRTIVRYTLARVFHDTRAITPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RIRRYEHCFGRRGMAGVLIRSARAIDPDSYGAITARYREIDIPTLIVWGKEDRIVRIGQG
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEECCCCCEEEECCC
KRLAEEMPDARLAVIDGCGHNPHEERPRETLAAIMEFLGNGEEREEGSGRFWREARR
CHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]