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The map label for this gene is 78221930

Identifier: 78221930

GI number: 78221930

Start: 780508

End: 781392

Strand: Reverse

Name: 78221930

Synonym: Gmet_0710

Alternate gene names: NA

Gene position: 781392-780508 (Counterclockwise)

Preceding gene: 78221931

Following gene: 78221929

Centisome position: 19.55

GC content: 63.05

Gene sequence:

>885_bases
ATGTCAAAGCTTACCGTCGGCCTCGTGCAGCAGAGCTGCACCAGCGATAAAGACCTGAATCTGGCTAAAAGCATCGAGAA
CATCCGCAAGGCTAGCGCCCTGGGCGCTAAACTCGTGGTGCTGCAGGAGCTCCACTGCGGCCCCTACTTCTGCCAGAACG
AGGACACCGGCCACTTCGACCTGGCCGAGGAGATCCCTGGCCCCACCACCGAACTACTGGGGGGCGTTGCCAGGGAGTTC
GGCGTGGTGCTTGTCTCTTCCCTCTTCGAGAAACGGGCGTCGGGGATTTACCACAACACCGCCGTGGTCTTCGAGAAAGA
CGGCTCCATCGCCGGGAAATACCGTAAGATGCACATCCCCGACGATCCCGGCTACTACGAGAAGTTCTACTTCACTCCCG
GCGACCTGGGTTTTGAGCCCATACGCACCTCGGTGGGAAAACTCGGCGTCCTCGTCTGCTGGGACCAGTGGTATCCCGAA
GCGGCCCGCCTCATGGCCCTTGCCGGAGCCGACCTCCTCATCTACCCCACGGCCATCGGCTGGGACCCGAGGGACGAGGA
CGAGGAGAAGGTACGCCAGAAAGAGGCCTGGATTACCATCCAGAGGGGGCACGCCGTGGCCAACGGCATACCGGTGGTGA
GCGTGAACCGTGTGGGGCTCGAACCCGACCCTTCCGGCGTGCTGCCGGGGAGCCTCTTCTGGGGTTCCAGCTTCGTGGCC
GGCCCCCAGGGTGAAATCCTCACCCAGGCCTCCAACGACCGGGAGGAACTCCTCTCTGTCGAGCTTGATCTTGACCGCAG
CGAGGCGGTGCGCCGCATCTGGCCGTTCCTGCGGGACCGGCGAATCGACGCCTATCAAGATCTGCTGAAGCGGTACCGGG
ACTGA

Upstream 100 bases:

>100_bases
CCCTCATCCTGCAACATGGCTCGCTCCACTGCGTGACCATGCAGCTGCCGAAGGGGACCCTTGCACCGGAACCACGAAAC
GTCACCAGAGAGGTATGAAA

Downstream 100 bases:

>100_bases
CCGCCATGGCACTTCAGGACAAGGAACTACTCGAAGCCCTCGCCAGCACCAAGATTCTCACCTCCATGATCACGCCGGCG
GTACTCATCTCCGCCGCCGG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSKLTVGLVQQSCTSDKDLNLAKSIENIRKASALGAKLVVLQELHCGPYFCQNEDTGHFDLAEEIPGPTTELLGGVAREF
GVVLVSSLFEKRASGIYHNTAVVFEKDGSIAGKYRKMHIPDDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPRDEDEEKVRQKEAWITIQRGHAVANGIPVVSVNRVGLEPDPSGVLPGSLFWGSSFVA
GPQGEILTQASNDREELLSVELDLDRSEAVRRIWPFLRDRRIDAYQDLLKRYRD

Sequences:

>Translated_294_residues
MSKLTVGLVQQSCTSDKDLNLAKSIENIRKASALGAKLVVLQELHCGPYFCQNEDTGHFDLAEEIPGPTTELLGGVAREF
GVVLVSSLFEKRASGIYHNTAVVFEKDGSIAGKYRKMHIPDDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPRDEDEEKVRQKEAWITIQRGHAVANGIPVVSVNRVGLEPDPSGVLPGSLFWGSSFVA
GPQGEILTQASNDREELLSVELDLDRSEAVRRIWPFLRDRRIDAYQDLLKRYRD
>Mature_293_residues
SKLTVGLVQQSCTSDKDLNLAKSIENIRKASALGAKLVVLQELHCGPYFCQNEDTGHFDLAEEIPGPTTELLGGVAREFG
VVLVSSLFEKRASGIYHNTAVVFEKDGSIAGKYRKMHIPDDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPEA
ARLMALAGADLLIYPTAIGWDPRDEDEEKVRQKEAWITIQRGHAVANGIPVVSVNRVGLEPDPSGVLPGSLFWGSSFVAG
PQGEILTQASNDREELLSVELDLDRSEAVRRIWPFLRDRRIDAYQDLLKRYRD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=297, Percent_Identity=29.2929292929293, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI7706509, Length=302, Percent_Identity=28.1456953642384, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI297632350, Length=293, Percent_Identity=27.6450511945392, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632348, Length=293, Percent_Identity=27.6450511945392, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI5031947, Length=293, Percent_Identity=27.6450511945392, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=30.7692307692308, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=272, Percent_Identity=30.5147058823529, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17556280, Length=288, Percent_Identity=25.3472222222222, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323383, Length=303, Percent_Identity=31.3531353135314, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21358471, Length=280, Percent_Identity=32.8571428571429, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI17933642, Length=286, Percent_Identity=26.2237762237762, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21355835, Length=291, Percent_Identity=26.8041237113402, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32743; Mature: 32612

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLTVGLVQQSCTSDKDLNLAKSIENIRKASALGAKLVVLQELHCGPYFCQNEDTGHFD
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHEEEHHHHCCCCEEECCCCCCCCC
LAEEIPGPTTELLGGVAREFGVVLVSSLFEKRASGIYHNTAVVFEKDGSIAGKYRKMHIP
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEEEECCCCCCCCEEEEECC
DDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPEAARLMALAGADLLIYPTAIG
CCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECC
WDPRDEDEEKVRQKEAWITIQRGHAVANGIPVVSVNRVGLEPDPSGVLPGSLFWGSSFVA
CCCCCCHHHHHHHHHHEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCC
GPQGEILTQASNDREELLSVELDLDRSEAVRRIWPFLRDRRIDAYQDLLKRYRD
CCCCCEEECCCCCHHHHEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKLTVGLVQQSCTSDKDLNLAKSIENIRKASALGAKLVVLQELHCGPYFCQNEDTGHFD
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHEEEHHHHCCCCEEECCCCCCCCC
LAEEIPGPTTELLGGVAREFGVVLVSSLFEKRASGIYHNTAVVFEKDGSIAGKYRKMHIP
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEEEECCCCCCCCEEEEECC
DDPGYYEKFYFTPGDLGFEPIRTSVGKLGVLVCWDQWYPEAARLMALAGADLLIYPTAIG
CCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECC
WDPRDEDEEKVRQKEAWITIQRGHAVANGIPVVSVNRVGLEPDPSGVLPGSLFWGSSFVA
CCCCCCHHHHHHHHHHEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCC
GPQGEILTQASNDREELLSVELDLDRSEAVRRIWPFLRDRRIDAYQDLLKRYRD
CCCCCEEECCCCCHHHHEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]