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The map label for this gene is aksA

Identifier: 78221912

GI number: 78221912

Start: 759067

End: 760218

Strand: Direct

Name: aksA

Synonym: Gmet_0692

Alternate gene names: 78221912

Gene position: 759067-760218 (Clockwise)

Preceding gene: 78221911

Following gene: 78221913

Centisome position: 18.99

GC content: 65.54

Gene sequence:

>1152_bases
ATGGAAAGCGCGACAAAGAACACAGTCATTATCGACGACACCACTCTGCGCGACGGCGAGCAGACCGCTGGCGTCGTTTT
CAGCAAAAGGGAAAAGCTGGCCATCGCCCGGCTGCTGGACGACATCGGGGTGCAGGAGCTGGAATGCGGCATCCCGGCCA
TGGGGGAGGAGGAGCGGGAGGTGATCCGTGCCCTGGTGGACCTGGGGCTCAACGCCCGACTCATCACCTGGAACCGGGCC
GTGATCCCCGACATCGAGGCATCCCTGGCCTGCGGCGTTGCGGCGGTGGACATCTCCCTCTCGGTCTCTGACCAGATGAT
TGCCCACAAACTCCGCACAAGCCGGGATGGGGTCATGGAGCAGCTGAAACGGGCCCTGGGCTTTGCCAAAGAACATGATC
TCTACGTCTCCGTCGGTGGGGAGGACGCGAGCCGGGCCGACCTCGGGTTCCTGGCCGAGCTCATGGAGATCACCTGCTCC
ATGGGGGGAGACCGCTTCCGCTTCTGCGACACTCTCGGCATTCTCGACCCGTTCACCATGTACGAGAAGGTGAAGGCCCT
GCGGGCTGCAGCCCCCGGCCTTGACATCGAGGTCCACACCCACAATGACCTGGGGATGGCCACAGCCAACGCCCTCGCCG
GCATCAAGGCTGGTGCCCGTTTTGTGAACACCACGGTGAACGGCCTCGGGGAGCGGGCAGGCAACGCGGCTCTGGAAGAG
GTGGTGATGGGGCTCAAGCTTGCCTGCGGCATCGACCCGGGGATCGACACCCACCGCTTCCACGAGATTTCCCGCTTCGT
TGGAAGGGCCTCCCAGCGGCCGGTCCCCCCCTGGAAGGCGGTAGTGGGGGAGCGGGTATTCTCCCATGAATCGGGGCTTC
ACGCCGACGGGGTCATCAAGGATGCCCGCAACTACGAGGGGTTCGATCCGGCCGAGGTGGGGCTTTGCCGACACATCGTG
GTGGGGAAGCATTCGGGGACCAGCGGCCTTGTGGAGCGTTTCCGGCAGATGGGGATTGAGCTCTCCCGGCAGGAGGCAGG
TGCTCTGATGGATGGGGTGCGGGCCATGGCCCAGCGGCTGAAGCGCCCCCTCTCCGACGGGGAGCTTCGAGCCCTCCATG
CCGCGGGGCGGTGCGCCCTCAGGGCGGCATGA

Upstream 100 bases:

>100_bases
GGCACGCCTTGTGCTTTAAAGTCAGGTACAATTCGAGATTAATCGGCAATGGCGCCCCGGAACATCAGTTCCGGGGCTTT
TTTTTCGAAAGGAGCCTGCC

Downstream 100 bases:

>100_bases
GAAGAATGGGGCACTCTGGAAATAATGAGTGCTTATATAATGTGCAAATGGAGGTGGCGTAAGCGATGCGCCCCCCCTTG
TTTTTTCTGTCCGGCACGCC

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MESATKNTVIIDDTTLRDGEQTAGVVFSKREKLAIARLLDDIGVQELECGIPAMGEEEREVIRALVDLGLNARLITWNRA
VIPDIEASLACGVAAVDISLSVSDQMIAHKLRTSRDGVMEQLKRALGFAKEHDLYVSVGGEDASRADLGFLAELMEITCS
MGGDRFRFCDTLGILDPFTMYEKVKALRAAAPGLDIEVHTHNDLGMATANALAGIKAGARFVNTTVNGLGERAGNAALEE
VVMGLKLACGIDPGIDTHRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIKDARNYEGFDPAEVGLCRHIV
VGKHSGTSGLVERFRQMGIELSRQEAGALMDGVRAMAQRLKRPLSDGELRALHAAGRCALRAA

Sequences:

>Translated_383_residues
MESATKNTVIIDDTTLRDGEQTAGVVFSKREKLAIARLLDDIGVQELECGIPAMGEEEREVIRALVDLGLNARLITWNRA
VIPDIEASLACGVAAVDISLSVSDQMIAHKLRTSRDGVMEQLKRALGFAKEHDLYVSVGGEDASRADLGFLAELMEITCS
MGGDRFRFCDTLGILDPFTMYEKVKALRAAAPGLDIEVHTHNDLGMATANALAGIKAGARFVNTTVNGLGERAGNAALEE
VVMGLKLACGIDPGIDTHRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIKDARNYEGFDPAEVGLCRHIV
VGKHSGTSGLVERFRQMGIELSRQEAGALMDGVRAMAQRLKRPLSDGELRALHAAGRCALRAA
>Mature_383_residues
MESATKNTVIIDDTTLRDGEQTAGVVFSKREKLAIARLLDDIGVQELECGIPAMGEEEREVIRALVDLGLNARLITWNRA
VIPDIEASLACGVAAVDISLSVSDQMIAHKLRTSRDGVMEQLKRALGFAKEHDLYVSVGGEDASRADLGFLAELMEITCS
MGGDRFRFCDTLGILDPFTMYEKVKALRAAAPGLDIEVHTHNDLGMATANALAGIKAGARFVNTTVNGLGERAGNAALEE
VVMGLKLACGIDPGIDTHRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIKDARNYEGFDPAEVGLCRHIV
VGKHSGTSGLVERFRQMGIELSRQEAGALMDGVRAMAQRLKRPLSDGELRALHAAGRCALRAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=277, Percent_Identity=25.6317689530686, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1786261, Length=380, Percent_Identity=34.4736842105263, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6320071, Length=379, Percent_Identity=28.2321899736148, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320019, Length=370, Percent_Identity=27.8378378378378, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324682, Length=403, Percent_Identity=23.5732009925558, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24582381, Length=302, Percent_Identity=25.8278145695364, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 41290; Mature: 41290

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESATKNTVIIDDTTLRDGEQTAGVVFSKREKLAIARLLDDIGVQELECGIPAMGEEERE
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHH
VIRALVDLGLNARLITWNRAVIPDIEASLACGVAAVDISLSVSDQMIAHKLRTSRDGVME
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHCCEEEEEEEECCHHHHHHHHHHCCHHHHHH
QLKRALGFAKEHDLYVSVGGEDASRADLGFLAELMEITCSMGGDRFRFCDTLGILDPFTM
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
YEKVKALRAAAPGLDIEVHTHNDLGMATANALAGIKAGARFVNTTVNGLGERAGNAALEE
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVMGLKLACGIDPGIDTHRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
DARNYEGFDPAEVGLCRHIVVGKHSGTSGLVERFRQMGIELSRQEAGALMDGVRAMAQRL
HHCCCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHH
KRPLSDGELRALHAAGRCALRAA
HCCCCCCCCHHHHHCCCEEECCC
>Mature Secondary Structure
MESATKNTVIIDDTTLRDGEQTAGVVFSKREKLAIARLLDDIGVQELECGIPAMGEEERE
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHH
VIRALVDLGLNARLITWNRAVIPDIEASLACGVAAVDISLSVSDQMIAHKLRTSRDGVME
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHCCEEEEEEEECCHHHHHHHHHHCCHHHHHH
QLKRALGFAKEHDLYVSVGGEDASRADLGFLAELMEITCSMGGDRFRFCDTLGILDPFTM
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
YEKVKALRAAAPGLDIEVHTHNDLGMATANALAGIKAGARFVNTTVNGLGERAGNAALEE
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVMGLKLACGIDPGIDTHRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
DARNYEGFDPAEVGLCRHIVVGKHSGTSGLVERFRQMGIELSRQEAGALMDGVRAMAQRL
HHCCCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHH
KRPLSDGELRALHAAGRCALRAA
HCCCCCCCCHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]