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The map label for this gene is rpoA

Identifier: 78221873

GI number: 78221873

Start: 713367

End: 714389

Strand: Direct

Name: rpoA

Synonym: Gmet_0653

Alternate gene names: 78221873

Gene position: 713367-714389 (Clockwise)

Preceding gene: 78221872

Following gene: 78221874

Centisome position: 17.85

GC content: 49.07

Gene sequence:

>1023_bases
ATGTACAGAAACTGGCGTGACCTGATCAGCCCCAAGAAGCTTCAGGTTGAGAGTGAATCGCTTACCAATACATACGGAAA
ATTTTTTGCTGAGCCCTTCGAACGTGGATTTGGAACGACACTCGGAAACTCGCTGCGAAGAGTGCTTCTTTCATCACTTC
AGGGTGCCGCGATTTCTTCCGTGAAAATTAAGGGAGTGCTCCACGAGTTTTCATCCATCCCCGGTGTGACTGAGGATGTT
ACGAATATCATACTCAATCTCAAAGGTGTCAGCCTCAAGATGCACGGAAATGAGGCCCGCACAGTACGTATTATTCACAA
AGGTGACGGGATTGTTAAGGCAGGCGATATTGTCACCGATGCAAATGTTGAAATTCTGAACCCAGACCACCATATTGCCA
CCTGTTCGAAGGATGCCAATCTGGAGATGGAGATGGTGGTAAAGCTGGGCAAGGGGTATGTGCCTTCGGATCGTAACCGT
GATGAGAAGGCTCCGGTTGGAACGATGCCGATCGATGCCATATTCTCTCCCATCAAGAAAGTGAATTTCACTGTCTCAAA
TGCTCGTGTAGGTCAAATGACCGACTATGACAAGCTGACTCTTGAAGTCTGGACGAACGGCAGTGTTGTTCCGGAAGATG
CTGTTGCGTTTGCTGCAAAGATTCTTAAGGAGCAACTGAGCATTTTTATCAACTTCGATGAAGAAGCCGAACCTGCTGAG
GAAGCGGAAACCGAGGAGGAGCGTGAACGGGTTAACGAGAACCTTTATCGCTCCGTAGACGAGCTCGAACTGTCCGTACG
CTCGGCAAACTGCCTCAAAAATGCCGGTATCAAGATGATTGGCGAACTTGTTTCGCGTTCCGAGGCTGAGATGCTCAAGA
CACAAAACTTCGGGCGCAAATCCCTGAACGAGATCAAGGATATTCTCGCAGATATGGGACTTACTCTCGGGATGAAGCTG
GATGGCTTCCCTGACCCTGAGGTTATGCGTAGGATCCGTGGGGAGCGGAAGGACGAAGAATAA

Upstream 100 bases:

>100_bases
CCCCGCTCGCGAGGACATTGCAATGCCGATTCAGGAGCAACTGATCGTCGAGCTTTACTCCAAGTAACAAGCGAACAGAC
CCGTAACAGGGAGGATATGC

Downstream 100 bases:

>100_bases
TTGCTAGGTTTCCTGAAGAAAGGATTATAGGTTATGCGTCACAATAAGGCAGGGAGAAGACTTGGAAGAACCACGAGCCA
CCGGATAGCGATGTTTCGTA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MYRNWRDLISPKKLQVESESLTNTYGKFFAEPFERGFGTTLGNSLRRVLLSSLQGAAISSVKIKGVLHEFSSIPGVTEDV
TNIILNLKGVSLKMHGNEARTVRIIHKGDGIVKAGDIVTDANVEILNPDHHIATCSKDANLEMEMVVKLGKGYVPSDRNR
DEKAPVGTMPIDAIFSPIKKVNFTVSNARVGQMTDYDKLTLEVWTNGSVVPEDAVAFAAKILKEQLSIFINFDEEAEPAE
EAETEEERERVNENLYRSVDELELSVRSANCLKNAGIKMIGELVSRSEAEMLKTQNFGRKSLNEIKDILADMGLTLGMKL
DGFPDPEVMRRIRGERKDEE

Sequences:

>Translated_340_residues
MYRNWRDLISPKKLQVESESLTNTYGKFFAEPFERGFGTTLGNSLRRVLLSSLQGAAISSVKIKGVLHEFSSIPGVTEDV
TNIILNLKGVSLKMHGNEARTVRIIHKGDGIVKAGDIVTDANVEILNPDHHIATCSKDANLEMEMVVKLGKGYVPSDRNR
DEKAPVGTMPIDAIFSPIKKVNFTVSNARVGQMTDYDKLTLEVWTNGSVVPEDAVAFAAKILKEQLSIFINFDEEAEPAE
EAETEEERERVNENLYRSVDELELSVRSANCLKNAGIKMIGELVSRSEAEMLKTQNFGRKSLNEIKDILADMGLTLGMKL
DGFPDPEVMRRIRGERKDEE
>Mature_340_residues
MYRNWRDLISPKKLQVESESLTNTYGKFFAEPFERGFGTTLGNSLRRVLLSSLQGAAISSVKIKGVLHEFSSIPGVTEDV
TNIILNLKGVSLKMHGNEARTVRIIHKGDGIVKAGDIVTDANVEILNPDHHIATCSKDANLEMEMVVKLGKGYVPSDRNR
DEKAPVGTMPIDAIFSPIKKVNFTVSNARVGQMTDYDKLTLEVWTNGSVVPEDAVAFAAKILKEQLSIFINFDEEAEPAE
EAETEEERERVNENLYRSVDELELSVRSANCLKNAGIKMIGELVSRSEAEMLKTQNFGRKSLNEIKDILADMGLTLGMKL
DGFPDPEVMRRIRGERKDEE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=313, Percent_Identity=45.6869009584665, Blast_Score=285, Evalue=4e-78,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_GEOMG (Q39XX9)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383620.1
- HSSP:   P20429
- ProteinModelPortal:   Q39XX9
- STRING:   Q39XX9
- GeneID:   3738302
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0653
- NMPDR:   fig|269799.3.peg.680
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q39XX9
- ProtClustDB:   PRK05182
- BioCyc:   GMET269799:GMET_0653-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37874; Mature: 37874

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRNWRDLISPKKLQVESESLTNTYGKFFAEPFERGFGTTLGNSLRRVLLSSLQGAAISS
CCCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
VKIKGVLHEFSSIPGVTEDVTNIILNLKGVSLKMHGNEARTVRIIHKGDGIVKAGDIVTD
HHHHHHHHHHHHCCCCHHHHHHHHHEECCEEEEEECCCCEEEEEEECCCCEEEECCEEEC
ANVEILNPDHHIATCSKDANLEMEMVVKLGKGYVPSDRNRDEKAPVGTMPIDAIFSPIKK
CCEEEECCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VNFTVSNARVGQMTDYDKLTLEVWTNGSVVPEDAVAFAAKILKEQLSIFINFDEEAEPAE
HEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHH
EAETEEERERVNENLYRSVDELELSVRSANCLKNAGIKMIGELVSRSEAEMLKTQNFGRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCHH
SLNEIKDILADMGLTLGMKLDGFPDPEVMRRIRGERKDEE
HHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYRNWRDLISPKKLQVESESLTNTYGKFFAEPFERGFGTTLGNSLRRVLLSSLQGAAISS
CCCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
VKIKGVLHEFSSIPGVTEDVTNIILNLKGVSLKMHGNEARTVRIIHKGDGIVKAGDIVTD
HHHHHHHHHHHHCCCCHHHHHHHHHEECCEEEEEECCCCEEEEEEECCCCEEEECCEEEC
ANVEILNPDHHIATCSKDANLEMEMVVKLGKGYVPSDRNRDEKAPVGTMPIDAIFSPIKK
CCEEEECCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VNFTVSNARVGQMTDYDKLTLEVWTNGSVVPEDAVAFAAKILKEQLSIFINFDEEAEPAE
HEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHH
EAETEEERERVNENLYRSVDELELSVRSANCLKNAGIKMIGELVSRSEAEMLKTQNFGRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCHH
SLNEIKDILADMGLTLGMKLDGFPDPEVMRRIRGERKDEE
HHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA