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The map label for this gene is truA

Identifier: 78221826

GI number: 78221826

Start: 676216

End: 676986

Strand: Direct

Name: truA

Synonym: Gmet_0605

Alternate gene names: 78221826

Gene position: 676216-676986 (Clockwise)

Preceding gene: 78221825

Following gene: 78221827

Centisome position: 16.92

GC content: 60.83

Gene sequence:

>771_bases
ATGAGCGGGACGCCCTCCAGGGCGTCCTTGATTTTTATGCATACCATTAAACTTGTTCTCGAGTACGACGGCACAAACTA
CGCGGGCTGGCAGATTCAGCCCAACGGCATCTCTGTCCAGCAGGTGGTCGAAGAGGCGTTGGCGAGGCTGCTGAAGGAAC
CGGTTCGCGTGCATTCCTCTGGCCGCACCGATGCCGGTGTCCACGCCCGGGGCATGGTTGCCGCCTTCACCACTGCCAAA
GGGCTGCCGATGCGGGCCTTCTCCGATGGGCTCAATGCGCTTCTTCCTCCCGATATCGCTGTGATGGAAGCGCTGGAGGC
TCCGGACGGCTTTAATCCGCGCTTTGACGCCGCAGGCAAGCACTACCGCTATACCATACACCGGGGGGAGCGCCGTTCTC
CCCTTTGCCGCCACTATGTCTGGCACGCGAGGGAGACGCTCGATCTGGCAGCCATGGAAAAGGGGGCCAGCCATATGGTC
GGTGAGCATGATTTCGCCTCTTTTCGCACAGCAGGTTGTGCCGCGCGGACCACAGTCCGACGGATAGATGCCGTGGACCT
GGCACTGGAAGGGGATCTCCTCCATATTGACGTAAAGGGTTCCGGATTCCTGCGCAACATGGTCCGGATCATGGTTGGCA
CCCTGGTGGAGGTTGGCCGGGGGAGGCGGAGTGCCGATGACGTGGCGCTTCTGTTTCGGTATCCTGGAGAACGTTTTGCC
GGCCCGACAGCCCCCCCTCAGGGGCTGTGCCTCATGGAAGTCTATTATTGA

Upstream 100 bases:

>100_bases
CCACGAATGCCGTCCAAATCGCCGAGATTCTGATCGAAAAGTATCTCAGGTAGGGCCTGTTCGTATATCCCCCAGCCGGG
ACGCATGGCAACATGTCACC

Downstream 100 bases:

>100_bases
CATATATTTTAGGGTTGACAGCTGCTGATAGCCATAGTATTATCCGTCCTTCGCTGAGAAAAGTGTTTTACTCTTTATTC
CATCCAGTTTGAGAGGGTTC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSGTPSRASLIFMHTIKLVLEYDGTNYAGWQIQPNGISVQQVVEEALARLLKEPVRVHSSGRTDAGVHARGMVAAFTTAK
GLPMRAFSDGLNALLPPDIAVMEALEAPDGFNPRFDAAGKHYRYTIHRGERRSPLCRHYVWHARETLDLAAMEKGASHMV
GEHDFASFRTAGCAARTTVRRIDAVDLALEGDLLHIDVKGSGFLRNMVRIMVGTLVEVGRGRRSADDVALLFRYPGERFA
GPTAPPQGLCLMEVYY

Sequences:

>Translated_256_residues
MSGTPSRASLIFMHTIKLVLEYDGTNYAGWQIQPNGISVQQVVEEALARLLKEPVRVHSSGRTDAGVHARGMVAAFTTAK
GLPMRAFSDGLNALLPPDIAVMEALEAPDGFNPRFDAAGKHYRYTIHRGERRSPLCRHYVWHARETLDLAAMEKGASHMV
GEHDFASFRTAGCAARTTVRRIDAVDLALEGDLLHIDVKGSGFLRNMVRIMVGTLVEVGRGRRSADDVALLFRYPGERFA
GPTAPPQGLCLMEVYY
>Mature_255_residues
SGTPSRASLIFMHTIKLVLEYDGTNYAGWQIQPNGISVQQVVEEALARLLKEPVRVHSSGRTDAGVHARGMVAAFTTAKG
LPMRAFSDGLNALLPPDIAVMEALEAPDGFNPRFDAAGKHYRYTIHRGERRSPLCRHYVWHARETLDLAAMEKGASHMVG
EHDFASFRTAGCAARTTVRRIDAVDLALEGDLLHIDVKGSGFLRNMVRIMVGTLVEVGRGRRSADDVALLFRYPGERFAG
PTAPPQGLCLMEVYY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=286, Percent_Identity=28.6713286713287, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1788657, Length=244, Percent_Identity=38.5245901639344, Blast_Score=167, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321112, Length=231, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28149; Mature: 28017

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTPSRASLIFMHTIKLVLEYDGTNYAGWQIQPNGISVQQVVEEALARLLKEPVRVHSS
CCCCCCHHHHHHHEEEEEHEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GRTDAGVHARGMVAAFTTAKGLPMRAFSDGLNALLPPDIAVMEALEAPDGFNPRFDAAGK
CCCCCCCCCCCCEEEEHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
HYRYTIHRGERRSPLCRHYVWHARETLDLAAMEKGASHMVGEHDFASFRTAGCAARTTVR
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHCCHHHHHHHH
RIDAVDLALEGDLLHIDVKGSGFLRNMVRIMVGTLVEVGRGRRSADDVALLFRYPGERFA
HHHHEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHCC
GPTAPPQGLCLMEVYY
CCCCCCCCEEEEEEEC
>Mature Secondary Structure 
SGTPSRASLIFMHTIKLVLEYDGTNYAGWQIQPNGISVQQVVEEALARLLKEPVRVHSS
CCCCCHHHHHHHEEEEEHEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GRTDAGVHARGMVAAFTTAKGLPMRAFSDGLNALLPPDIAVMEALEAPDGFNPRFDAAGK
CCCCCCCCCCCCEEEEHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
HYRYTIHRGERRSPLCRHYVWHARETLDLAAMEKGASHMVGEHDFASFRTAGCAARTTVR
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHCCHHHHHHHH
RIDAVDLALEGDLLHIDVKGSGFLRNMVRIMVGTLVEVGRGRRSADDVALLFRYPGERFA
HHHHEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHCC
GPTAPPQGLCLMEVYY
CCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA