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The map label for this gene is bchE [H]

Identifier: 78221818

GI number: 78221818

Start: 663892

End: 665397

Strand: Direct

Name: bchE [H]

Synonym: Gmet_0597

Alternate gene names: 78221818

Gene position: 663892-665397 (Clockwise)

Preceding gene: 78221817

Following gene: 78221819

Centisome position: 16.61

GC content: 60.09

Gene sequence:

>1506_bases
ATGAAAATCAAGAGAATAGCCCTCATTACCCCCCCCTACCACGCGGGCGTTGTGGAGTCGGCCGGCACCTGGCTCAATGT
CGGTTTTGTCTACATCGCCGGGGCACTCAGAGCGGCGGGGTACGAGGTGGATTACTACGATGCCATGTCTCTGTGGCACA
AGTGGCCCGACATCCAGAAGCGGATCGAGGCGTTCCGCCCCGATATGGTTGCCACGACCGCCTATACCGCCTCCATTGTC
GAAGCGGTGAAACTGCTCCGGTTCGCCAAGTCCATCAACCCCCGCATCGTGACGGCGCTCGGCAACGTCCACGCGGCCTT
CTGTTACGACGAGGTTCTTACCCACGACCACGACGCCGTGGACTACATCGTCAGGGGGGAGGGGGAGGAGACGCTCCCCA
TGCTCTGCCACTGCCTCAATGCCGGCGATGACCCGAAGAAGGTTCTCGGAATCGCCTACTGGCGCGACGGCGGGGTGGTG
GTTACCCCCAAGGCCCCGTACATTCATGACCTGGACAATCTTCCCACAGCCTGGGATCTGGTGGAGTGGCCCATCTATAC
CTACCGGGCCAAGAAGAACGCCCGGCTGGCCATCGTCTCCTCTTCCCGCGGCTGCAAATCCCAGTGTTCCTTCTGTTCCC
AGCAGCTCTTCTGGGCCCAGTCATGGCGCGCCCGGAGCGCCGAGAACTTCGTGGCCGAGCTGGAGATGCTCCACAACGTC
CACGGTGTCCAGGTGGCCATGCTCTCCGACGAGCTTCCCACCTTCGACCGGGAGCGGTGGGTCCGGATCCTGGATCTCAT
GGTGGAACGGGAAGTGCCGGTGAAGCTTCTCATGGAGACCCGGGTGGACGACATTCTGCGGGACGCCGATATCATGGACA
AGTACCGGGAAGGTGGCGTTGAGCACATCTATGTGGGGGTCGAGGCGGGAAGCCAGGAAACCCTCGACCTCTTCAAGAAG
GATACGGAAGTTGCCCAGTCCAAGCAGGCCATCGACCTGATTAATGGGGCCGACATCGTTTCGGAAACCTCTTTCGTCCT
CGGCATGCCCGACGACACCCCTGAATCCATTGAGCAGACCATCGAGCTCGCCAAGCACTACAACCCTGACATGGCGTTCT
TTCTTGCCATCGCCCCCTGGCCCTATGCCGAGCTCTACCCCGAGCTCGAGCCCTACGTGGCCACCAAGGATTACCGCAAA
TACAACCTGGTGGAGCCGGTCATCAAGCCCAAAAACATGACCATGGAAGAACTGGAGCGCCAGTTGGGGAAAGCATCCCA
GAAGTTCTTCATGCACAAGTTCCAGAACCTGGACAAGCTCTCCCCCTGGAAGCAGGAGTTCATGCTGTCGGTCCTCGATC
TCCTCATTAACCACTCCTATCTGGCCGGCCAGATGAAGGCGATGCTCAAGGAGGGGAAGGAGATGCCCGCGGAGGTCAAG
GCGCTCCTGAAAACGGTCAGCCAGCACAAGCATGTCCCCCACCAGACGATAGCGCCCATTCCATGA

Upstream 100 bases:

>100_bases
CGTTTTATAAGCAGAATGTGCATAAGCTAATGCTCTTTGGGGGCGGCCCGATTTTTGCTTTTTGTCTGGCTCAGTTCCCA
ACCTCGTCGAGGAGAAGTGA

Downstream 100 bases:

>100_bases
GTTCTTTCCGTTTCACTGTACCGAACCGAACTCTGGCATGTGGAATGCTAAATAGGTATTCAGTTGCAGCAAAATGTTTG
AAATCAGTATGGGATGTTTA

Product: radical SAM family protein

Products: NA

Alternate protein names: Anaerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKIKRIALITPPYHAGVVESAGTWLNVGFVYIAGALRAAGYEVDYYDAMSLWHKWPDIQKRIEAFRPDMVATTAYTASIV
EAVKLLRFAKSINPRIVTALGNVHAAFCYDEVLTHDHDAVDYIVRGEGEETLPMLCHCLNAGDDPKKVLGIAYWRDGGVV
VTPKAPYIHDLDNLPTAWDLVEWPIYTYRAKKNARLAIVSSSRGCKSQCSFCSQQLFWAQSWRARSAENFVAELEMLHNV
HGVQVAMLSDELPTFDRERWVRILDLMVEREVPVKLLMETRVDDILRDADIMDKYREGGVEHIYVGVEAGSQETLDLFKK
DTEVAQSKQAIDLINGADIVSETSFVLGMPDDTPESIEQTIELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRK
YNLVEPVIKPKNMTMEELERQLGKASQKFFMHKFQNLDKLSPWKQEFMLSVLDLLINHSYLAGQMKAMLKEGKEMPAEVK
ALLKTVSQHKHVPHQTIAPIP

Sequences:

>Translated_501_residues
MKIKRIALITPPYHAGVVESAGTWLNVGFVYIAGALRAAGYEVDYYDAMSLWHKWPDIQKRIEAFRPDMVATTAYTASIV
EAVKLLRFAKSINPRIVTALGNVHAAFCYDEVLTHDHDAVDYIVRGEGEETLPMLCHCLNAGDDPKKVLGIAYWRDGGVV
VTPKAPYIHDLDNLPTAWDLVEWPIYTYRAKKNARLAIVSSSRGCKSQCSFCSQQLFWAQSWRARSAENFVAELEMLHNV
HGVQVAMLSDELPTFDRERWVRILDLMVEREVPVKLLMETRVDDILRDADIMDKYREGGVEHIYVGVEAGSQETLDLFKK
DTEVAQSKQAIDLINGADIVSETSFVLGMPDDTPESIEQTIELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRK
YNLVEPVIKPKNMTMEELERQLGKASQKFFMHKFQNLDKLSPWKQEFMLSVLDLLINHSYLAGQMKAMLKEGKEMPAEVK
ALLKTVSQHKHVPHQTIAPIP
>Mature_501_residues
MKIKRIALITPPYHAGVVESAGTWLNVGFVYIAGALRAAGYEVDYYDAMSLWHKWPDIQKRIEAFRPDMVATTAYTASIV
EAVKLLRFAKSINPRIVTALGNVHAAFCYDEVLTHDHDAVDYIVRGEGEETLPMLCHCLNAGDDPKKVLGIAYWRDGGVV
VTPKAPYIHDLDNLPTAWDLVEWPIYTYRAKKNARLAIVSSSRGCKSQCSFCSQQLFWAQSWRARSAENFVAELEMLHNV
HGVQVAMLSDELPTFDRERWVRILDLMVEREVPVKLLMETRVDDILRDADIMDKYREGGVEHIYVGVEAGSQETLDLFKK
DTEVAQSKQAIDLINGADIVSETSFVLGMPDDTPESIEQTIELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRK
YNLVEPVIKPKNMTMEELERQLGKASQKFFMHKFQNLDKLSPWKQEFMLSVLDLLINHSYLAGQMKAMLKEGKEMPAEVK
ALLKTVSQHKHVPHQTIAPIP

Specific function: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis in anaerobic conditions. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-prot

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011772
- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 56978; Mature: 56978

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRIALITPPYHAGVVESAGTWLNVGFVYIAGALRAAGYEVDYYDAMSLWHKWPDIQK
CCEEEEEEECCCCCCCCHHCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHH
RIEAFRPDMVATTAYTASIVEAVKLLRFAKSINPRIVTALGNVHAAFCYDEVLTHDHDAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCE
DYIVRGEGEETLPMLCHCLNAGDDPKKVLGIAYWRDGGVVVTPKAPYIHDLDNLPTAWDL
EEEEECCCCCHHHHHHHHHCCCCCHHHHEEEEEEECCCEEECCCCCCCCCCCCCCCHHHH
VEWPIYTYRAKKNARLAIVSSSRGCKSQCSFCSQQLFWAQSWRARSAENFVAELEMLHNV
HHCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
HGVQVAMLSDELPTFDRERWVRILDLMVEREVPVKLLMETRVDDILRDADIMDKYREGGV
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EHIYVGVEAGSQETLDLFKKDTEVAQSKQAIDLINGADIVSETSFVLGMPDDTPESIEQT
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCHHHHHHH
IELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRKYNLVEPVIKPKNMTMEELER
HHHHHHCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QLGKASQKFFMHKFQNLDKLSPWKQEFMLSVLDLLINHSYLAGQMKAMLKEGKEMPAEVK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
ALLKTVSQHKHVPHQTIAPIP
HHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKIKRIALITPPYHAGVVESAGTWLNVGFVYIAGALRAAGYEVDYYDAMSLWHKWPDIQK
CCEEEEEEECCCCCCCCHHCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHH
RIEAFRPDMVATTAYTASIVEAVKLLRFAKSINPRIVTALGNVHAAFCYDEVLTHDHDAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCE
DYIVRGEGEETLPMLCHCLNAGDDPKKVLGIAYWRDGGVVVTPKAPYIHDLDNLPTAWDL
EEEEECCCCCHHHHHHHHHCCCCCHHHHEEEEEEECCCEEECCCCCCCCCCCCCCCHHHH
VEWPIYTYRAKKNARLAIVSSSRGCKSQCSFCSQQLFWAQSWRARSAENFVAELEMLHNV
HHCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
HGVQVAMLSDELPTFDRERWVRILDLMVEREVPVKLLMETRVDDILRDADIMDKYREGGV
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EHIYVGVEAGSQETLDLFKKDTEVAQSKQAIDLINGADIVSETSFVLGMPDDTPESIEQT
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCHHHHHHH
IELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRKYNLVEPVIKPKNMTMEELER
HHHHHHCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QLGKASQKFFMHKFQNLDKLSPWKQEFMLSVLDLLINHSYLAGQMKAMLKEGKEMPAEVK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
ALLKTVSQHKHVPHQTIAPIP
HHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8385667; 2203738; 10841582 [H]