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The map label for this gene is speE [H]

Identifier: 78221804

GI number: 78221804

Start: 646169

End: 648682

Strand: Direct

Name: speE [H]

Synonym: Gmet_0583

Alternate gene names: 78221804

Gene position: 646169-648682 (Clockwise)

Preceding gene: 78221803

Following gene: 78221805

Centisome position: 16.16

GC content: 62.41

Gene sequence:

>2514_bases
GTGACGAAGCCTCTACGGCCCGGAGTTCAGCTCCTGCTCTGCCTGATTGCCTATTTCTCCTCCGGTGCGGCGAGCCTCTG
TGCGGAAGTTACCTGGAACCGGATGCTCATCGTGGTGGTGGGCAATTCCCTCGCCGCGACCTCCATGATCGTCGTGGTCT
TCATGGGGGGGCTCGGCCTCGGGGGGTATGCCGGCGGGAAGCTCCTGGCGCGCCGCCGGTGCTCGCTGCTTCCCTACGCG
ATCCTTGAAGTTCTCATTGCCGCCTACATCCTCCTCTCGCCAATCCTCTTCGACCTCCTTGCCGGTGCCTTCTCCTCCCT
GGCCGAGCAGTGGGAACAGCCGGCGCTGCTGAGCCTGCTGCGGTTTGCCGTCACCGTGGTGGCCCTGTTGATCCCGGCGA
CCCTCATGGGGGCGACGTTCCCCGCCATCATGACCGGGGTGCGATCCAGGTATCCCGAAAACAGCCATGCGCTCCTCTAT
GGCGTGAACACCGTGGGGGCGGCGGTGGGCTGTTTCGTGGGGGGGTATCATCTGCTCTTCGAACTGGGGGTTCAGGCAAC
GCTCTGGCTGGCCGCCGGACTTGGCGGCGTATCAGTCCTCTGTGTGGCCGTGGCAGCGGTTCTGTCTCCGTCTCGGGCCT
GTTTCCAGCTCGCTCCGGAGCCGGAGGTGGCCGACGAAGGCACTTCTGCTTCCGGCGAGGGGGGGCTGTTCCGGTTCCTG
GCGGTTTCCTCCTTCCTAGTTGGGTTTGTGGCCCTTGCCTACGAGGTGCTGGTCACCCGGATGGTGATTCTCTTTCTGAA
CAATCTGGCATCCACGTTCGCGCTGGCCCTGACCGGCTTCCTGCTGGGGACCGGCGCCGGGGCGCTGCTGGCCAACGGCG
CCCATGCCGCAAATAACAGGATCGGCGGGAACGGGGTGCGCCTGTTCGGGGCCATTGCCCTGTTGTCGGGCATCCTGCTA
GCTGCGACCCCCTATGCTATCATGGCGGCCAGCTACCCGCTGCAGGCCCTCGGCATCATCGTCATCCCGATGGTTTTTCT
CGGCTCGCTGCTCCCCTTCGCCATCCGGACCCTGCACGCGAGGGATCGCGAAGCCGCGATCCGGGGGACCTCCTTCCTGT
ACGCCGCCAATACGGTCGGCGGGATGCTGGGGGCCGGCCTCATCAATTATCAGCTTCTGCCGAGGATCGGCCTGCAGGGA
TCGGTCGGGCTCCTGTTCGGCATCTGCGCCGCCATTGCCGTCGCCTGCTTCGTCCCGTCCCTGACCCGCGGCTTCAGGTG
GGCGACCCTGCTGCTGGTTCCGGCGCTCATGCCGCTCATCACGCTGAATGCCCTGCCGAACATCATGAACCGCTATGCCC
TGAAGATCGCCGAGTGGACCGGGGCCCCCGAGGTTACGATCAGGCTTATTCACGAAGGGCGCGCCGCCAACGTGACGGTG
CTCGATCAGAACGATCCGAAGCAGGGGGAATTCCGGGACATGTATCTGAACGGCATCGAGGAGGCGTCGACCCGCTTCTA
CCACGCCCAGCTCTTCAAGCTCCTGGGGATGCTTCCGGTTGTCGTCCATGGGGCCGAACGGCCCCAAAACGCCCTCGTCA
TCGCCTTCGGCTCGGGGATTACCGCCGGGTCGGTCCTGGCGTCCGATATGATCTCTTCCCTCGATGTGGTCGATCTGAAC
CCCGATGTGGAGGGGATCAACAATCTGTTCAAGGAATTCAACGGCGACGTGTTCCACGAGCCCCGCTTCCGCTTTCACAA
TGACGATGGCAGAAATTATCTGGTGACCAGCGGGAAAAAATACGATCTGATCATCAGCGACTCGACCCACCCCTGCTCCT
ACGACAGCTGGATCCTGTACACCCGGGAGTTTTATCGCGATGTGAAGCGGCGCCTCCCCCCGGGCGGCGTCTTTGCCCAG
TGGGTCTCCGTCTCCGAGATCATGCGGGGCGAGCTCTTCCCGATCTATCTGAATACCTTCCGGAGCGTCTTTCCCCATGC
GACCTTCTGGTATGTTTACGGGTCGAACCAGGCCCTGATGCTCGCCACGCCGGAACCGTTCAGGCTCGATGCCGGCAAGC
TCCAGAAGCGCCTGGACCGGCTCGCCCCCTGGTTCAGGGCCGGGGAATACCAGCTCGATTCAGTCGCGAAGGTGGCGGGC
TTCTTCTGGCTTGATGAAGAGATGATGGGCCGGATGATCGGCGCCGAGACCCGTGTGAACACGGATATGCGCCATTATTT
CGAGAAGTCCTCCATGCAGGCCCAAATCCCGCCGCAACGGCAGCTTCCCTTTTTCCAGGCCGATGTCACGCGGTATCTCA
CCGGTGGTGATGAACGGCTGCGCAGCGACATCCGAAAGGAGCAGAAGACGGGGTTGCTGCTGACCAGGTATGGTTTCTAC
AACGATATCAGAGACCTTGGCCAAGCTTACTGTTTAAGTCCTGAGAACAATAACGTAAAATACTGGATGAGATTCGCCTA
TTCCGGAAATATCCCCGCGGGACTGTGCCCCTAG

Upstream 100 bases:

>100_bases
GATGTGTCGGCCACAATGGACAGCGGCAAGAACGCGCTGGATATAGCCCGAGCCAGGGGAAATGCCGCCATCGTGGAACT
GCTGGAAAAGGGACGATAAC

Downstream 100 bases:

>100_bases
ACATGCCATTCTTTTTGGGTGAGCCGAAAAAATACGCGAAGCACGCCGCAGGCATTGCCGCAGCATGGGCCGCGCTTATC
TGCCTGGCGGTGTACCTGCG

Product: spermine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 837; Mature: 836

Protein sequence:

>837_residues
MTKPLRPGVQLLLCLIAYFSSGAASLCAEVTWNRMLIVVVGNSLAATSMIVVVFMGGLGLGGYAGGKLLARRRCSLLPYA
ILEVLIAAYILLSPILFDLLAGAFSSLAEQWEQPALLSLLRFAVTVVALLIPATLMGATFPAIMTGVRSRYPENSHALLY
GVNTVGAAVGCFVGGYHLLFELGVQATLWLAAGLGGVSVLCVAVAAVLSPSRACFQLAPEPEVADEGTSASGEGGLFRFL
AVSSFLVGFVALAYEVLVTRMVILFLNNLASTFALALTGFLLGTGAGALLANGAHAANNRIGGNGVRLFGAIALLSGILL
AATPYAIMAASYPLQALGIIVIPMVFLGSLLPFAIRTLHARDREAAIRGTSFLYAANTVGGMLGAGLINYQLLPRIGLQG
SVGLLFGICAAIAVACFVPSLTRGFRWATLLLVPALMPLITLNALPNIMNRYALKIAEWTGAPEVTIRLIHEGRAANVTV
LDQNDPKQGEFRDMYLNGIEEASTRFYHAQLFKLLGMLPVVVHGAERPQNALVIAFGSGITAGSVLASDMISSLDVVDLN
PDVEGINNLFKEFNGDVFHEPRFRFHNDDGRNYLVTSGKKYDLIISDSTHPCSYDSWILYTREFYRDVKRRLPPGGVFAQ
WVSVSEIMRGELFPIYLNTFRSVFPHATFWYVYGSNQALMLATPEPFRLDAGKLQKRLDRLAPWFRAGEYQLDSVAKVAG
FFWLDEEMMGRMIGAETRVNTDMRHYFEKSSMQAQIPPQRQLPFFQADVTRYLTGGDERLRSDIRKEQKTGLLLTRYGFY
NDIRDLGQAYCLSPENNNVKYWMRFAYSGNIPAGLCP

Sequences:

>Translated_837_residues
MTKPLRPGVQLLLCLIAYFSSGAASLCAEVTWNRMLIVVVGNSLAATSMIVVVFMGGLGLGGYAGGKLLARRRCSLLPYA
ILEVLIAAYILLSPILFDLLAGAFSSLAEQWEQPALLSLLRFAVTVVALLIPATLMGATFPAIMTGVRSRYPENSHALLY
GVNTVGAAVGCFVGGYHLLFELGVQATLWLAAGLGGVSVLCVAVAAVLSPSRACFQLAPEPEVADEGTSASGEGGLFRFL
AVSSFLVGFVALAYEVLVTRMVILFLNNLASTFALALTGFLLGTGAGALLANGAHAANNRIGGNGVRLFGAIALLSGILL
AATPYAIMAASYPLQALGIIVIPMVFLGSLLPFAIRTLHARDREAAIRGTSFLYAANTVGGMLGAGLINYQLLPRIGLQG
SVGLLFGICAAIAVACFVPSLTRGFRWATLLLVPALMPLITLNALPNIMNRYALKIAEWTGAPEVTIRLIHEGRAANVTV
LDQNDPKQGEFRDMYLNGIEEASTRFYHAQLFKLLGMLPVVVHGAERPQNALVIAFGSGITAGSVLASDMISSLDVVDLN
PDVEGINNLFKEFNGDVFHEPRFRFHNDDGRNYLVTSGKKYDLIISDSTHPCSYDSWILYTREFYRDVKRRLPPGGVFAQ
WVSVSEIMRGELFPIYLNTFRSVFPHATFWYVYGSNQALMLATPEPFRLDAGKLQKRLDRLAPWFRAGEYQLDSVAKVAG
FFWLDEEMMGRMIGAETRVNTDMRHYFEKSSMQAQIPPQRQLPFFQADVTRYLTGGDERLRSDIRKEQKTGLLLTRYGFY
NDIRDLGQAYCLSPENNNVKYWMRFAYSGNIPAGLCP
>Mature_836_residues
TKPLRPGVQLLLCLIAYFSSGAASLCAEVTWNRMLIVVVGNSLAATSMIVVVFMGGLGLGGYAGGKLLARRRCSLLPYAI
LEVLIAAYILLSPILFDLLAGAFSSLAEQWEQPALLSLLRFAVTVVALLIPATLMGATFPAIMTGVRSRYPENSHALLYG
VNTVGAAVGCFVGGYHLLFELGVQATLWLAAGLGGVSVLCVAVAAVLSPSRACFQLAPEPEVADEGTSASGEGGLFRFLA
VSSFLVGFVALAYEVLVTRMVILFLNNLASTFALALTGFLLGTGAGALLANGAHAANNRIGGNGVRLFGAIALLSGILLA
ATPYAIMAASYPLQALGIIVIPMVFLGSLLPFAIRTLHARDREAAIRGTSFLYAANTVGGMLGAGLINYQLLPRIGLQGS
VGLLFGICAAIAVACFVPSLTRGFRWATLLLVPALMPLITLNALPNIMNRYALKIAEWTGAPEVTIRLIHEGRAANVTVL
DQNDPKQGEFRDMYLNGIEEASTRFYHAQLFKLLGMLPVVVHGAERPQNALVIAFGSGITAGSVLASDMISSLDVVDLNP
DVEGINNLFKEFNGDVFHEPRFRFHNDDGRNYLVTSGKKYDLIISDSTHPCSYDSWILYTREFYRDVKRRLPPGGVFAQW
VSVSEIMRGELFPIYLNTFRSVFPHATFWYVYGSNQALMLATPEPFRLDAGKLQKRLDRLAPWFRAGEYQLDSVAKVAGF
FWLDEEMMGRMIGAETRVNTDMRHYFEKSSMQAQIPPQRQLPFFQADVTRYLTGGDERLRSDIRKEQKTGLLLTRYGFYN
DIRDLGQAYCLSPENNNVKYWMRFAYSGNIPAGLCP

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786312, Length=167, Percent_Identity=27.5449101796407, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 91113; Mature: 90982

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPLRPGVQLLLCLIAYFSSGAASLCAEVTWNRMLIVVVGNSLAATSMIVVVFMGGLGL
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCC
GGYAGGKLLARRRCSLLPYAILEVLIAAYILLSPILFDLLAGAFSSLAEQWEQPALLSLL
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RFAVTVVALLIPATLMGATFPAIMTGVRSRYPENSHALLYGVNTVGAAVGCFVGGYHLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHH
ELGVQATLWLAAGLGGVSVLCVAVAAVLSPSRACFQLAPEPEVADEGTSASGEGGLFRFL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCCCCCCCHHHHH
AVSSFLVGFVALAYEVLVTRMVILFLNNLASTFALALTGFLLGTGAGALLANGAHAANNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHCCCC
IGGNGVRLFGAIALLSGILLAATPYAIMAASYPLQALGIIVIPMVFLGSLLPFAIRTLHA
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDREAAIRGTSFLYAANTVGGMLGAGLINYQLLPRIGLQGSVGLLFGICAAIAVACFVPS
CCHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LTRGFRWATLLLVPALMPLITLNALPNIMNRYALKIAEWTGAPEVTIRLIHEGRAANVTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEE
LDQNDPKQGEFRDMYLNGIEEASTRFYHAQLFKLLGMLPVVVHGAERPQNALVIAFGSGI
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
TAGSVLASDMISSLDVVDLNPDVEGINNLFKEFNGDVFHEPRFRFHNDDGRNYLVTSGKK
CHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCEECCCCCEECCCCCEEEEECCCE
YDLIISDSTHPCSYDSWILYTREFYRDVKRRLPPGGVFAQWVSVSEIMRGELFPIYLNTF
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
RSVFPHATFWYVYGSNQALMLATPEPFRLDAGKLQKRLDRLAPWFRAGEYQLDSVAKVAG
HHHCCCCEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHCCCCCHHHHHHHHH
FFWLDEEMMGRMIGAETRVNTDMRHYFEKSSMQAQIPPQRQLPFFQADVTRYLTGGDERL
HHEECHHHHHHHHCCCHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
RSDIRKEQKTGLLLTRYGFYNDIRDLGQAYCLSPENNNVKYWMRFAYSGNIPAGLCP
HHHHHHHHHCCEEEEECCCHHHHHHHHHHEECCCCCCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TKPLRPGVQLLLCLIAYFSSGAASLCAEVTWNRMLIVVVGNSLAATSMIVVVFMGGLGL
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCC
GGYAGGKLLARRRCSLLPYAILEVLIAAYILLSPILFDLLAGAFSSLAEQWEQPALLSLL
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RFAVTVVALLIPATLMGATFPAIMTGVRSRYPENSHALLYGVNTVGAAVGCFVGGYHLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHH
ELGVQATLWLAAGLGGVSVLCVAVAAVLSPSRACFQLAPEPEVADEGTSASGEGGLFRFL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCCCCCCCHHHHH
AVSSFLVGFVALAYEVLVTRMVILFLNNLASTFALALTGFLLGTGAGALLANGAHAANNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHCCCC
IGGNGVRLFGAIALLSGILLAATPYAIMAASYPLQALGIIVIPMVFLGSLLPFAIRTLHA
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDREAAIRGTSFLYAANTVGGMLGAGLINYQLLPRIGLQGSVGLLFGICAAIAVACFVPS
CCHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LTRGFRWATLLLVPALMPLITLNALPNIMNRYALKIAEWTGAPEVTIRLIHEGRAANVTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEE
LDQNDPKQGEFRDMYLNGIEEASTRFYHAQLFKLLGMLPVVVHGAERPQNALVIAFGSGI
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
TAGSVLASDMISSLDVVDLNPDVEGINNLFKEFNGDVFHEPRFRFHNDDGRNYLVTSGKK
CHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCEECCCCCEECCCCCEEEEECCCE
YDLIISDSTHPCSYDSWILYTREFYRDVKRRLPPGGVFAQWVSVSEIMRGELFPIYLNTF
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
RSVFPHATFWYVYGSNQALMLATPEPFRLDAGKLQKRLDRLAPWFRAGEYQLDSVAKVAG
HHHCCCCEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHCCCCCHHHHHHHHH
FFWLDEEMMGRMIGAETRVNTDMRHYFEKSSMQAQIPPQRQLPFFQADVTRYLTGGDERL
HHEECHHHHHHHHCCCHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
RSDIRKEQKTGLLLTRYGFYNDIRDLGQAYCLSPENNNVKYWMRFAYSGNIPAGLCP
HHHHHHHHHCCEEEEECCCHHHHHHHHHHEECCCCCCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]